SAKURAI Kaori

Activity information

Name and contact details

• Name

  サクライ　カオリ, 櫻井　香里, SAKURAI Kaori

• E-mail

  sakuraikcc.tuat.ac.jp

• External link

  http://web.tuat.ac.jp/~sakurai/

Affiliation / Position

• Institute of Engineering　Division of Biotechnology and Life Science, Professor

Other affiliation

• Faculty of Engineering　Department of Biotechnology and Life Science
• Graduate School of Engineering　Department of Biotechnology and Life Sciences

Current state of research and teaching activities

• 生物活性分子がどのように働いているかを解明できれば、新しい生物機能の発見や、新しい創薬の実現につながります。私たちは、生物活性分子から化学プローブを精密にデザインし、分子とタンパク質との相互作用を直接解析する手法を開発しています。

Research Areas

• Nanotechnology/Materials, Bio chemistry

Teaching Experience

• 生命工学ビジネス特別講義(3学期集中・集中・工学府博士前期)
  2024, 専門科目等
• 生命分子反応特論(1学期・木2・工学府博士後期)
  2024, 専門科目等
• 先端ブレインストーミング・イン・イングリシュ
  2024, 専門科目等
• Frontiers of BiotechnologyII
  2024, 専門科目等
• 生命工学の最先端IV
  2024, 専門科目等
• 生命工学実験II
  2024, 専門科目等
• ブレインストーミング・イン・イングリシュ(3学期集中・集中・工学府博士前期)
  2024, 専門科目等
• 生命工学入門・医工学入門
  2024, 専門科目等
• ケミカルバイオロジー(1学期・月1・工学部)
  2024, 専門科目等
• 応用生物工学プレゼンテーション特論Ⅰ(通年・集中・工学府博士前期)
  2024, 専門科目等
• 応用生物工学プレゼンテーション特論Ⅰ(年度跨り・集中・工学府博士前期)
  2024, 専門科目等
• 応用生物工学プレゼンテーション特論Ⅱ(通年・集中・工学府博士前期)
  2024, 専門科目等
• 生物有機化学特論(3学期・木1・工学府博士前期)
  2024, 専門科目等
• 生命有機化学Ⅰ(3学期・月2・工学部)
  2024, 専門科目等
• 生命工学実験II
  2023, 専門科目等
• 生命工学入門・医工学入門
  2023, 専門科目等
• 生命工学の最先端IV
  2023, 専門科目等
• 先端ブレインストーミング・イン・イングリシュ
  2023, 専門科目等

Grants-in-Aid for Scientific Research

• 基盤研究（S）
  巨大複雑天然物の高機能化：全合成・大規模ライブラリー構築・機能分子創製の革新
  From 2024, To 2024
• 基盤研究（S）
  巨大複雑天然物の高機能化：全合成・大規模ライブラリー構築・機能分子創製の革新
  From 2023, To 2023
• 基盤研究(S)
  巨大複雑天然物の高機能化：全合成・大規模ライブラリー構築・機能分子創製の革新
  From 2022, To 2022
• 基盤研究(C)
  未知標的タンパク質探索のための新規基盤技術の確立
  From 2021, To 2023
• 基盤研究(S)
  巨大複雑天然物群の網羅的創出による未踏創薬モレキュラースペースの開拓
  From 2021, To 2021
• 基盤研究(S)
  巨大複雑天然物群の網羅的創出による未踏創薬モレキュラースペースの開拓
  From 2020, To 2020
• 基盤研究(S)
  巨大複雑天然物群の網羅的創出による未踏創薬モレキュラースペースの開拓
  From 2019, To 2019
• 基盤研究(C)
  金ナノ粒子を用いた医薬品標的タンパク質の効率的探索法の開発
  From 2018, To 2020
• 基盤研究(S)
  巨大複雑天然物群の網羅的創出による未踏創薬モレキュラースペースの開拓
  From 2018, To 2018
• 基盤研究(S)
  巨大複雑天然物群の網羅的創出による未踏創薬モレキュラースペースの開拓
  From 2017, To 2017
• 基盤研究(S)
  生体モデル膜における脂質分子の動的配座とドメイン構造
  From 2017, To 2017
• 基盤研究（S）
  生体モデル膜における脂質分子の動的配座とドメイン構造
  From 2016, To 2016
• 基盤研究(C)
  低発現量タンパク質の探索を可能とするフォトアフィニティーセレクション法の開発
  From 2015, To 2017
• 挑戦的萌芽研究
  活性型/不活性型2種プローブシステムを用いた天然物結合タンパク質探索法の開発
  From 2013, To 2014
• 若手研究(B)
  生理活性糖脂質分子を特異的に認識する新規合成プローブの創製
  From 2007, To 2008

Papers

• Effect of Linker Length on the Function of Biotinylated OSW-1 Probes
  Khine, M., Isogai N., Takeshita, T. Sakurai, K
  Chembiochem
  02 Jan. 2025, Research paper (scientific journal), DOI(公開)(r-map)
• Analysis of OSW-1-Induced Stress Responses in HT-29 Cells
  Hibiki Nakamura, Yuma Ito, Mahmoud Kandeel, Kaori Sakurai, Kentaro Oh-hashi
  BPB Reports
  15 Jan. 2025, Research paper (scientific journal), joint, DOI(公開)(r-map)
• Anthocyanin glucosylation mediated by a glycoside hydrolase family 3 protein in purple carrot.
  10. Koga, S., Miyahara, T., Nishizaki, Y., Tamura, K., Okamoto, E., Kawagishi, H., Sakurai, K., Kaneko, Y., Kumakubo, R., Tanaka, T., Ozeki, Y., Sasaki, N.
  Plant J.
  20 Jun. 2024, Research paper (scientific journal), DOI(公開)(r-map)
• Dysregulation of PI4P in the trans Golgi regions activates the mammalian Golgi stress response.
  8. Kanae Sasaki, Takuya Adachi, Fumi Morishita, Marika Toide, Yuto Watanabe, Hajime Tajima Sakurai, Sadao Wakabayashi, Satoshi Kusumi, Toshiyuki Yamaji, Kaori Sakurai, Daisuke Koga, Kentaro Hanada, Masafumi Yohda, Hiderou Yoshida,
  J. Biol. Chem.
  13 Dec. 2024, Research paper (scientific journal), joint, DOI(公開)(r-map)
• Dichlorotriazine-based multivalent probe for selective affinity labeling of carbohydrate-binding proteins
  Tsuruno, Ayaka; Kamoshita, Shione; Hosoya, Shoichi; Sakurai, Kaori
  ORGANIC & BIOMOLECULAR CHEMISTRY
  ROYAL SOC CHEMISTRY
  A new series of multivalent gold nanoparticle probes bearing different electrophilic groups were synthesized and their affinity labeling reactivities were evaluated. The dichlorotriazine group was identified as a useful protein-reactive label, allowing selective capture of a target protein at nanomolar probe concentrations. The dichlorotriazine group was identified as a useful protein-reactive label, allowing selective capture of a target protein at nanomolar probe concentrations.
  25 Sep. 2024, Research paper (scientific journal), joint, 22, 37, 1477-0520, DOI(公開)(r-map), 7659, 7663
• Golgi-Targeting Anticancer Natural Products
  Khine, Myat Nyein; Sakurai, Kaori
  CANCERS
  MDPI
  The Golgi apparatus plays an important role in maintaining cell homeostasis by serving as a biosynthetic center for glycans, lipids and post-translationally modified proteins and as a sorting center for vesicular transport of proteins to specific destinations. Moreover, it provides a signaling hub that facilitates not only membrane trafficking processes but also cellular response pathways to various types of stresses. Altered signaling at the Golgi apparatus has emerged as a key regulator of tumor growth and survival. Among the small molecules that can specifically perturb or modulate Golgi proteins and organization, natural products with anticancer property have been identified as powerful chemical probes in deciphering Golgi-related pathways and, in particular, recently described Golgi stress response pathways. In this review, we highlight a set of Golgi-targeting natural products that enabled the characterization of the Golgi-mediated signaling events leading to cancer cell death and discuss the potential for selectively exploiting these pathways for the development of novel chemotherapeutic agents.
  Apr. 2023, Research paper (scientific journal), joint, 15, 7, DOI(公開)(r-map)
• Elucidation of OSW-1-Induced Stress Responses in Neuro2a Cells
  Oh-hashi, Kentaro; Nakamura, Hibiki; Ogawa, Hirotaka; Hirata, Yoko; Sakurai, Kaori
  INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
  MDPI
  OSW-1, a steroidal saponin isolated from the bulbs of Ornithogalum saundersiae, is a promising compound for an anticancer drug; however, its cytotoxic mechanisms have not been fully elucidated. Therefore, we analyzed the stress responses triggered by OSW-1 in the mouse neuroblastoma cell line Neuro2a by comparing it with brefeldin A (BFA), a Golgi apparatus-disrupting reagent. Among the Golgi stress sensors TFE3/TFEB and CREB3, OSW-1 induced dephosphorylation of TFE3/TFEB but not cleavage of CREB3, and induction of the ER stress-inducible genes GADD153 and GADD34 was slight. On the other hand, the induction of LC3-II, an autophagy marker, was more pronounced than the BFA stimulation. To elucidate OSW-1-induced gene expression, we performed a comprehensive gene analysis using a microarray method and observed changes in numerous genes involved in lipid metabolism, such as cholesterol, and in the regulation of the ER-Golgi apparatus. Abnormalities in ER-Golgi transport were also evident in the examination of secretory activity using NanoLuc-tag genes. Finally, we established Neuro2a cells lacking oxysterol-binding protein (OSBP), which were severely reduced by OSW-1, but found OSBP deficiency had little effect on OSW-1-induced cell death and the LC3-II/LC3-I ratio in Neuro2a cells. Future work to elucidate the relationship between OSW-1-induced atypical Golgi stress responses and autophagy induction may lead to the development of new anticancer agents.
  Mar. 2023, Research paper (scientific journal), joint, 24, 6, DOI(公開)(r-map)
• Chemical probe-based approach to discovery of target proteins of natural products
  Kaori Sakurai
  Journal of The Society of Japanese Women Scientists
  The Society of Japanese Women Scientists
  30 Jan. 2023, Research paper (scientific journal), 23, DOI(公開)(r-map), 22, 30
• Deciphering the Biological Activities of Antifungal Agents with Chemical Probes
  Fridman, Micha; Sakurai, Kaori
  ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
  WILEY-V C H VERLAG GMBH
  The growing number of fungal infections caused by pathogens resistant to one or more classes of antifungal drugs emphasizes the threat that these microorganisms pose to animal and human health and global food security. Open questions remain regarding the mechanisms of action of the limited repertoire of antifungal agents, making it challenging to rationally develop more efficacious therapeutics. In recent years, the use of chemical biology approaches has resolved some of these questions and has provided new promising concepts to guide the design of antifungal agents. By focusing on examples from studies carried out in recent years, this minireview describes the key roles that probes based on antifungal agents and their derivatives have played in uncovering details about their activities, in detecting resistance, and in characterizing the interactions between these agents and their targets.
  26 Jan. 2023, Research paper (scientific journal), joint, 1433-7851, DOI(公開)(r-map)
• Reactivity analysis of new multivalent electrophilic probes for affinity labeling of carbohydrate binding proteins
  Kamoshita, S., Suto, N., Sakurai, K.*
  ChemBioChem
  WILEY-V C H VERLAG GMBH
  We have designed and synthesized six different multivalent electrophiles as carbohydrate affinity labeling probes. Evaluation of the reactivity of the electrophiles against peanut agglutinin (PNA) and Ricinus communis agglutinin (RCA) showed that p- and m-aryl sulfonyl fluoride are effective protein reactive groups that label carbohydrate binding lectins in a ligand-dependent fashion at a nanomolar probe concentration. Analysis of the selectivity of affinity labeling in the presence of excess BSA as a nonspecific protein indicated that m-arylsulfonyl fluoride is a more selective protein-reactive group, albeit with attenuated reactivity. Further analysis showed that the labeling efficiency of the multivalent electrophilic probes can be improved by employing reaction conditions involving 25 degrees C instead of typically employed 4 degrees C. Both isomers of arylsulfonyl fluoride groups together represent promising affinity labels for target identification studies that could serve as more efficient alternatives to photoreactive groups.
  16 Feb. 2022, Research paper (scientific journal), joint, 23, 4, 1439-4227, DOI(公開)(r-map)
• Exploration of the Reactivity of Multivalent Electrophiles for Affinity Labeling: Sulfonyl Fluoride as a Highly Efficient and Selective Label
  Suto, Nanako; Kamoshita, Shione; Hosoya, Shoichi; Sakurai, Kaori
  ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
  WILEY-V C H VERLAG GMBH
  Here we explored the reactivity of a set of multivalent electrophiles cofunctionalized with a carbohydrate ligand on gold nanoparticles to achieve efficient affinity labeling for target protein analysis. Evaluation of the reactivity and selectivity of the electrophiles against three different cognate binding proteins identified arylsulfonyl fluoride as the most efficient protein-reactive group in this study. We demonstrated that multivalent arylsulfonyl fluoride probe 4 at 50 nm concentration achieved selective affinity labeling and enrichment of a model protein PNA in cell lysate, which was more effective than photoaffinity probe 1 with arylazide group. Labeling site analysis by LC-MS/MS revealed that the nano-particle-immobilized arylsulfonyl fluoride group can target multiple amino acid residues around the ligand binding site of the target proteins. Our study highlights the utility of arylsulfonyl fluoride as a highly effective multivalent affinity label suitable for covalently capturing unknown target proteins.
  26 Jul. 2021, Research paper (scientific journal), joint, 60, 31, 1433-7851, DOI(公開)(r-map), 17080, 17087
• Clickable gold-nanoparticles as generic probe precursors for facile photoaffinity labeling application
  Mori, Kanna; Sakurai, Kaori
  ORGANIC & BIOMOLECULAR CHEMISTRY
  ROYAL SOC CHEMISTRY
  Rapid access to appropriately functionalized probes is crucial in chemical labeling approaches to target identification studies. We designed and synthesized clickable gold-nanoparticles as generic probe precursors that enable (1) one-step ligand derivatization by click chemistry, and (2) facile photoaffinity labeling application. Using cholesterol as a model ligand, we successfully demonstrated the utility of the ligand-clicked probe in photoaffinity labeling of endogenously expressed oxysterol-binding protein (OSBP) in cell lysate.
  14 Feb. 2021, Research paper (scientific journal), joint, 19, 6, 1477-0520, DOI(公開)(r-map), 1268, 1273
• Interactions of OSW-1 with Lipid Bilayers in Comparison with Digitonin and Soyasaponin
  Malabed, Raymond; Hanashima, Shinya; Murata, Michio; Sakurai, Kaori
  LANGMUIR
  AMER CHEMICAL SOC
  OSW-1, a unique steroidal saponin isolated from the bulbs of Ornithogalum saundersiae, has potent cell-growth inhibition activity. In this study, we conducted fluorescence measurements and microscopic observations using palmitoyloleoylphosphatidylcholine (POPC)-cholesterol (Chol) bilayers to evaluate the membrane-binding affinity of OSW-1 in comparison with another steroidal saponin, digitonin, and the triterpenoid saponin, soyasaponin Bb(I). The membrane activities of these saponins were evaluated using calcein leakage assays and fitted to the binding isotherm by changing the ratios of saponin-lipids. Digitonin showed the highest binding affinity for the POPC-Chol membrane (K-app = 0.38 mu M-1) and the strongest membrane disruptivity in the bound saponin-lipid ratio at the point of 50% calcein leakage (r(50) = 0.47) occurrence. OSW-1 showed slightly lower activity (Kapp = 0.31 mu M-1; r(50) = 0.78), and the soyasaponin was the lowest in the membrane affinity and the calcein leakage activity (K-app = 0.017 mu M-1; r(50) = 1.66). The effect of OSW-1 was further assessed using confocal microscopy in an experiment utilizing DiI and rhodamine 6G as the fluorescence probes. The addition of 30 mu M OSW-1 induced inward membrane curvature in some giant unilamellar vesicles (GUVs). At the higher OSW-1 concentration (58 mu M, r(50) = 0.78) where the 50% calcein leakage was observed, the morphology of some GUVs became elongated. With digitonin at the corresponding concentration (35 mu M, r(50) = 0.47), membrane disruption and formation of large aggregates in aqueous solution were observed, probably due to a detergent-type mechanism. These saponins, including OSW-1, required Chol to exhibit their potent membrane activity although their mechanisms are thought to be different. At the effective concentration, OSW-1 preferably binds to the bilayers without prominent disruption of vesicles and exerts its activity through the formation of saponin-Chol complexes, probably resulting in membrane permeabilization.
  07 Apr. 2020, Research paper (scientific journal), joint, 36, 13, 0743-7463, DOI(公開)(r-map), 3600, 3610
• Phospholipid-Dependent Functions of a Macrocyclic Analogue of the Ion-Channel-Forming Antibiotic Gramicidin A
  Mao, Ji; Itoh, Hiroaki; Sakurai, Kaori; Inoue, Masayuki
  CHEMICAL & PHARMACEUTICAL BULLETIN
  PHARMACEUTICAL SOC JAPAN
  An ion-channel-forming natural peptide, gramicidin A (1), exhibits potent antimicrobial activity against Gram-positive bacteria, although medical applications are limited to topical use due to its mammalian cytotoxicity. We recently reported that the artificial macrocyclic analogue 2 provides a promising starting point for developing new ion-channel-based systemic antibacterial agents because of its low mammalian cytotoxicity compared to that of the parent 1. To dissect the molecular factors involved in the species selectivity of 2, we evaluated the ion transport activities, phospholipid affinities, and conformational properties of 1 and 2 using various compositions of phospholipids. A combination of lipid dot blot assays and circular dichroism (CD) analysis with H+/Na+ exchange assays revealed that the higher H+/Na+ exchange activity of 2 than that of 1 in liposomes containing 1-palmitoy1-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) or 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG) is attributable to its higher affinity towards the phospholipids than that of 1. Notably, we also discovered that 2 showed weaker H+/Na+ exchange activity in liposomes containing 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylethanolamine (POPE). CD analysis of 2 in liposomes indicated that the weak H+/Na+ exchange activity is induced by disturbance of the ion-conducting beta(6.3)-helical conformation in the POPE-containing lipid bilayer. These results suggest that the POPE-induced attenuation of the ion -conducting activity of 2 contributes to the alleviation of undesirable mammalian cytotoxicity of 2 compared to that of 1.
  Feb. 2020, Research paper (scientific journal), joint, 68, 2, 0009-2363, DOI(公開)(r-map), 173, 178
• Computational and Experimental Analysis on the Conformational Preferences of Anticancer Saponin OSW-1
  Fukaya, Keisuke; Urabe, Daisuke; Hiraizumi, Masato; Noguchi, Keiichi; Matsumoto, Takashi; Sakurai, Kaori
  JOURNAL OF ORGANIC CHEMISTRY
  AMER CHEMICAL SOC
  The conformational properties of anticancer saponin OSW-1 were investigated by X-ray crystallography and by an integrated approach combining a conformational search and the evaluation of the computed conformational distribution by comparing the experimental and simulated spectroscopic data. Our results suggested that OSW-1 adopts two preferred conformations in solution at an approximately 2:1 ratio, of which the crystal structure is consistent with the major conformation. In the solution models, the arabinose residue of OSW-1 appears to serve as a molecular hinge by flipping from the standard C-4(1) form in the major conformer to the unusual C-1(4) form in the minor conformer. This results in different orientations of the biologically essential p-methoxybenzoyl group, thereby inducing a dramatic alteration of the three-dimensional shape and polarity of OSW-1.
  17 Jan. 2020, Research paper (scientific journal), joint, 85, 2, 0022-3263, DOI(公開)(r-map), 339, 344
• Clickable gold nanoparticles for streamlining capture, enrichment and release of alkyne-labelled proteins.
  Narita, S., Kobayashi, N., Mori, K. Sakurai, K.*
  Bioorg. Med. Chem. Lett.
  Elsevier
  15 Dec. 2019, Research paper (scientific journal), joint, 29, 24, DOI(公開)(r-map), 126768
• Development of Chemical Probes for Functional Analysis of Anticancer Saponin OSW-1
  Komatsu, Rina; Sakurai, Kaori
  CHEMICAL RECORD
  WILEY-V C H VERLAG GMBH
  Chemical probe-based approaches have proven powerful in recent years in the target identification studies of natural products. OSW-1 is a saponin class of natural products with highly potent and selective cytotoxicity against various cancer cell lines. Understanding its mechanism of action is important for the development of anticancer drugs with potentially novel target pathways. This account reviews recent progress in the development of OSW-1 derived probes for exploring the mechanism of its action. The key to the probe development is a judicious choice of functionalization sites and a selective functionalization strategy. The types of probes include fluorescent probes for cellular imaging analysis and affinity probes for target identification analysis.
  11 Dec. 2019, Research paper (scientific journal), joint, 19, 12, 1527-8999, DOI(公開)(r-map), 2362, 2369
• Anticancer saponin OSW-1 is a novel class of selective Golgi stress inducer
  Kimura, Mayu; Sasaki, Kanae; Fukutani, Yosuke; Yoshida, Hiderou; Ohsawa, Ikuroh; Yohda, Masafumi; Sakurai, Kaori
  BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
  PERGAMON-ELSEVIER SCIENCE LTD
  OSW-1 is a plant-derived natural product proposed to selectively kill cancer cells by binding to members of the oxysterol binding protein family, thereby disrupting lipid/sterol homeostasis. However, how these protein-ligand interactions mediate cell death signaling has remained elusive. Here, we discovered that OSW-1 selectively activates the Golgi stress response leading to apoptosis, providing a mechanistic basis for the anticancer activity of OSW-1.
  15 Jul. 2019, Research paper (scientific journal), joint, 29, 14, 0960-894X, DOI(公開)(r-map), 1732, 1736
• Design and synthesis of small molecule-conjugated photoaffinity nanoprobes for a streamlined analysis of binding proteins.
  Sakurai, K.*, Kato, A., Adachi, K.
  Bioorg. Med. Chem. Lett.
  Elsevier
  15 Oct. 2018, Research paper (scientific journal), joint, 28, DOI(公開)(r-map), 3227-3230
• Target identification of Yaku’amide B and its two distinct activities against mitochondrial FoF1-ATP synthase.
  Kitamura, K., Itoh, H., Sakurai, K., Dan, S., Inoue, M.*
  J. Am. Chem. Soc.
  American Chemical Society
  26 Sep. 2018, Research paper (scientific journal), joint, 140, DOI(公開)(r-map), 12189-12199
• Synthesis of alkyne-tagged and biotin-tagged Sortin1 as novel photoaffinity probes.
  Komatsu, R. Yamaguchi, T. Kobayashi, N. Ozeki, Y. Sakurai, K.
  Bioorg. Med. Chem. Lett.
  Elsevier
  15 May 2018, Research paper (scientific journal), joint, 28, DOI(公開)(r-map), 1562, 1565
• Sterol-recognition ability and membrane-disrupting activity of Ornithogalum saponin OSW-1 and usual 3-O-glycosyl saponins
  Malabed, Raymond; Hanashima, Shinya; Murata, Michio; Sakurai, Kaori
  BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
  ELSEVIER SCIENCE BV
  OSW-1 is a structurally unique steroidal saponin isolated from the bulbs of Ornithogalum saundersiae, and has exhibited highly potent and selective cytotoxicity in tumor cell lines. This study aimed to investigate the molecular mechanism for the membrane-permeabilizing activity of OSW-1 in comparison with those of other saponins by using various spectroscopic approaches. The membrane effects and hemolytic activity of OSW-1 were markedly enhanced in the presence of membrane cholesterol. Binding affinity measurements using fluorescent cholestatrienol and solid-state NMR spectroscopy of a 3-d-cholesterol probe suggested that OSW-1 interacts with membrane cholesterol without forming large aggregates while 3-O-glycosyl saponin, digitonin, forms cholesterol-containing aggregates. The results suggest that OSW-1/cholesterol interaction is likely to cause membrane permeabilization and pore formation without destroying the whole membrane integrity, which could partly be responsible for its highly potent cell toxicity.
  Dec. 2017, Research paper (scientific journal), joint, 1859, 12, 0005-2736, DOI(公開)(r-map), 2516, 2525
• A peptide-glycolipid interaction probed by retroinverso peptide analogues
  Sakurai, K.
  Chem. Pharm. Bull.
  Dec. 2017, Research paper (scientific journal), only, 66, 45, 50
• Dissecting the structural basis for the intracellular delivery of OSW-1 by fluorescent probes
  Hiraizumi, M.; Komatsu, R.; Shibata, T.; Ohta, Y.; Sakurai, K.
  ORGANIC & BIOMOLECULAR CHEMISTRY
  ROYAL SOC CHEMISTRY
  The structural basis for the intracellular delivery of OSW-1 is investigated using fluorescent derivatives of OSW-1 and its closely related congeners. Despite the large differences in activity, all the fluorescent probes are found to translocate across the plasma membrane to the ER and Golgi apparatus. This observation suggests that the glycosylated cholestane moiety plays an important role in the cell internalization and intracellular localization property of OSW-1.
  May 2017, Research paper (scientific journal), joint, 15, 17, 1477-0520, DOI(公開)(r-map), 3568, 3570
• Synthesis of a fluorescent photoaffinity probe of OSW-1 by site-selective acylation of an inactive congener and biological evaluation
  Sakurai, K.; Hiraizumi, M.; Isogai, N.; Komatsu, R.; Shibata, T.; Ohta, Y.
  CHEMICAL COMMUNICATIONS
  ROYAL SOC CHEMISTRY
  A novel fluorescent photoaffinity probe of OSW-1 was prepared in two steps from a naturally occurring inactive congener by a sequential site-selective acylation strategy using Me2SnCl2. It displayed highly potent anticancer activity and a similar intracellular localization property to that of a fluorescently-tagged OSW-1, thereby demonstrating its potential utility in live cell studies.
  Jan. 2017, Research paper (scientific journal), joint, 53, 3, 1359-7345, DOI(公開)(r-map), 517, 520
• Design and Synthesis of Fluorescent Glycolipid Photoaffinity Probes and Their Photoreactivity.
  Sakurai, K., Yamaguchi, T., Mizuno, S.
  Bioorganic and Medicinal Chemistry Letters
  Sep. 2016, Research paper (scientific journal), joint, 26, 5110, 5115
• Two-Step Synthesis of a Clickable Photoaffinity Probe From an Anticancer Saponin OSW-1 and Its Photochemical Reactivity
  Yamada, R., Hiraizumi, M., Narita, S., Sakurai, K.
  Asian J. Org. Chem.
  Jan. 2016, Research paper (scientific journal), joint, 5, 330, 334
• Gold Nanoparticle-Based Multivalent Carbohydrate Probes: Selective Photoaffinity Labeling Studies of the Carbohydrate-Binding Proteins with Different Affinities
  Sakurai, K. Hatai, Y., Okada, A.
  Chem. Sci.
  Jan. 2016, Research paper (scientific journal), joint, 7, 702, 706.
• Photoaffinity Labeling Studies of the Carbohydrate Binding Proteins with Different Affinities
  Sakurai, K. Ozawa, S. Yamaguchi, T.
  Bioorg. Med. Chem.
  Jul. 2015, Research paper (scientific journal), joint, 23, 5319, 5325
• Comparative Analysis of Diazirine-Based Photoaffinity Probes on Their Reactivity Toward a Carbohydrate-Binding Protein
  Sakurai, K., Yasui, T., Mizuno, S.
  Asian J. Org. Chem.
  May 2015, Research paper (scientific journal), joint, 4, 724, 728
• Photoaffinity Probes for identificaiton of Carbohydrate-Binding Proteins
  Sakurai, K.
  Asian. J. org. Chem.
  Jan. 2015, Research paper (scientific journal), only, 4, 116, 126
• Photoaffinity Labeling Approaches Toward Identification of Carbohydrate-Lectin Interactions
  Sakurai, K
  Trends Glycosci. Glycotech.
  Jan. 2015, Research paper (scientific journal), only, 27, 1, 12
• Synthesis of OSW-1 Derivatives by Site-Selective Acylation and Their Biological Evaluation
  Sakurai, K., Takeshita, T., Hiraizumi, M., Yamada, R.
  Organic Letters
  Dec. 2014, Research paper (scientific journal), joint, 16, 6318, 6321
• Comparison of the Reactivity of Carbohydrate Photoaffinity Probes with Different Photoreactive Groups
  Sakurai, K., Ozawa, M., Yamada, R., Yasui, T., Mizuno, S.
  ChemBioChem
  May 2014, Research paper (scientific journal), joint, 15, 1399, 1403
• Fluorescent Analog of OSW-1 and its Cellular Localization
  Yamada, R., Takeshita, T., Hiraizumi, M., Shinohe, D., Ohta, Y., M.Sakurai, K.,
  Bioorganic and Medicinal Chemistry Letters
  Feb. 2014, Research paper (scientific journal), joint, 24, 1839, 1842
• フォトアフィニティープローブシステムを用いた生物活性分子― タンパク質相互作用解析へのアプローチ
  櫻井香里
  生命化学研究レター
  Feb. 2013, only, 41, 9, 13
• Selective Fluorescence Detection of Small-Molecule-Binding Proteins by Using a Dual Photoaffinity Labeling System
  Sakurai, K., Yamada, R., Okada, A., Tawa M., S. Ozawa, M., Inoue, M.
  ChemBioChem
  Feb. 2013, Research paper (scientific journal), joint, 14, 421, 425
• Active/Inactive Dual-Probe Approach to Selective Photoaffinity Labeling of Small-Molecule Binding Proteins
  Sakurai, K. Tawa M., Okada, A., Yamada, R., Sato, N., Inahara, M., Inoue, M.
  Chemistry-Asian J.
  Jun. 2012, Research paper (scientific journal), joint, 7, 7, 1567–1571
• Three-Dimensional Structures of OSW-1 and Its Active Congener
  Kaori Sakurai,* Takuro Fukumoto, Keiichi Noguchi, Noriyuki Sato, Hiroki Asaka, Naomi Moriyama and Masafumi Yohda
  Organic Letters
  Nov. 2010, Research paper (scientific journal), joint, 12, 5732–5735
• In Vitro Selection of a DNA-Templated Small-Molecule Library Reveals a Class of Macrocyclic Kinase Inhibitors
  Kleiner, R., Dumelin, C., Tiu, G., Sakurai, K., Liu, D. R.
  J. Am. Chem. Soc.
  Aug. 2010, Research paper (scientific journal), joint, 132, 11779–11791
• Design and Synthesis of Functionalized Trisaccharides as p53-Peptide Mimics
  Kaori Sakurai, Daniel Kahne
  Tetrahedron Lett.
  May 2010, Research paper (scientific journal), joint, 51, 3724, 3727
• 1,5-Di-(4-methoxyphenyl)-3-hexahydrocyclopentaquinolylbiuret
  Kaori Sakurai, Keiichi Noguchi, Koichiro Nishibe
  Acta Crystallographica Section E
  Feb. 2010, Research paper (scientific journal), joint, E66, o655
• DNA-Templated Organic Synthesis (DTS): A Novel Approach to the Discovery of Functional Small Molecules Based on Nature’s Evolutionary Principles
  Kaori Sakurai David R. Liu
  Journal of Synthetic Organic Chemistry Japan
  Jun. 2008, joint, 66, 590, 604
• Crystallographic Analysis of an 8-mer p53 Peptide Analogue Complexed with MDM2
  Sakurai, K., Schubert, C., Kahne, D.
  The Journal of the American Chemical Society
  Aug. 2006, Research paper (scientific journal), joint, 128, 34, DOI(公開)(r-map), 11000, 11001
• DNA-templated functional group transformations enable sequence-programmed synthesis using small molecule reagents
  Sakurai, K., Snyder, T. M., Liu, D. R.
  J. Am. Chem. Soc.
  22 Jan. 2005, Research paper (scientific journal), joint, 127, DOI(公開)(r-map), 1660, 1661
• Use of a Retroinverso p53 Peptide as an Inhibitor of MDM2
  Sakurai, K., Chung, H. S., Kahne, D.
  J. Am. Chem. Soc.
  22 Dec. 2004, Research paper (scientific journal), joint, 126, DOI(公開)(r-map), 16288, 16289
• Reaction discovery enabled by DNA-templated synthesis and in vitro selection
  30Kanan, M.W., Rozenman, M. M., Sakurai, K., Snyder, T. M., Liu. D. R.
  Nature
  04 Sep. 2004, Research paper (scientific journal), joint, 431, DOI(公開)(r-map), 545, 549
• Scavenging byproducts in the sulfoxide glycosylation reaction: Application to the synthesis of ciclamycin 0
  Gildersleeve, J., Smith, A., Sakurai, K., Raghavan, S., Kahne, D.
  J. Am. Chem. Soc.
  16 Jun. 1999, Research paper (scientific journal), joint, 121, DOI(公開)(r-map), 6176, 6182

Books and Other Publications

• ケミカルバイオロジー化合物周ー研究展開のヒントー
  日本学術振興会　ケミカルバイオロジー第189委員会編
  4章 4.10 抗癌剤を志向したステロイドサポニン系化合物
  オーム社
  23 Oct. 2018, 978-4-274-50709-0

Presentations

• Analysis of binding proteins of a peptidic natural product yaku’amide B,
  日本薬学会大145年会
  27 Mar. 2025, Oral presentation(general)
• Functional analysis of anticancer saponin OSW-1 using biotinylated probe.
  日本化学会第105春季年会
  29 Mar. 2025, Oral presentation(general)
• Photoaffinity labeling with natural product-functionalized gold-nanoparticle probes.
  日本化学会第105春季年会
  29 Mar. 2025, Oral presentation(general)
• 金ナノ粒子プローブを用いた標的タンパク質ラベリングシステムの開発.
  日本化学会第104春季年会
  21 Mar. 2024, Oral presentation(general)
• Development of gold-nanoparticle photoaffinity probes for target identification of natural products.
  日本化学会第104春季年会
  20 Mar. 2024, Poster presentation
• Design and synthesis of hydrophobically functionalized gold-nanoparticle probes for target validation.
  The 3rd International Symposium of Biofunctional Chemistry (ISBC2024)
  26 Apr. 2024, Poster presentation
• Protein binding activities of anticancer saponin OSW-1,
  The 3rd International Symposium of Biofunctional Chemistry (ISBC2024)
  26 Apr. 2024, Poster presentation
• Development of natural product-functionalized gold-nanoparticle photoprobes
  The 3rd International Symposium of Biofunctional Chemistry (ISBC2024)
  26 Apr. 2024, Poster presentation
• Synthesis of hydrophobically functionalized gold-nanoparticle probes for target validation.
  日本ケミカルバイオロジー学会第18回年会,
  27 May 2024, Poster presentation
• Protein abundance and binding interaction analysis of the oxysterol-binding Protein (OSBP)-Related Protein (ORP) family members.
  日本ケミカルバイオロジー学会第18回年会
  27 May 2024, Poster presentation
• Development of natural product-functionalized gold-nanoparticle photoprobes. .
  第11回バイオ関連化学シンポジウム若手フォーラム,
  11 Sep. 2024, Poster presentation
• Functional analysis of anticancer saponin OSW-1 using biotinylated probe.
  第43回日本糖質学会年会,
  14 Sep. 2024, Poster presentation
• 標的同定に向けた天然物修飾金ナノ粒子フォトプローブの開ポスター発表.
  学術変革領域研究「天然物が織りなす化合物潜在空間が拓く生物活性分子デザイン」第1回リトリート
  18 Nov. 2024, Poster presentation
• マルチバレントプローブによる標的タンパク質の効率的検出技術の開発.
  学術変革領域研究（A） 潜在空間分子設計「天然物が織りなす化合物潜在空間が拓く生物活性分子デザイン」第1回リトリート
  17 Nov. 2024, Poster presentation
• 天然物の作用機構解明に向けた化学プローブの開発.
  東京大学大学院講新領域創成科学研究科先端生命科学専攻　Departmental Seminar, 大学院講義「生命科学概論I/II」,
  19 Jan. 2024, Public discourse, seminar, tutorial, course, lecture and others
• マルチバレントプローブによる標的タンパク質の効率的検出技術の開発.
  第28回埼玉大学先端産業国際ラボラトリーメディカルイノベーション研究ユニット（MiU）ワークショップ
  12 Jul. 2024, Oral presentation(invited, special)
• マルチバレントプローブによる標的タンパク質の効率的検出技術の開発
  学術変革領域研究（A） 潜在空間分子設計第２回公開シンポジウム
  12 Jun. 2024, Poster presentation
• Multivalent probes for exploring carbohydrate-binding proteins.
  Departmental Seminar, Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden,
  26 Feb. 2025, Oral presentation(invited, special)
• Multivalent probes for exploring carbohydrate-binding proteins.
  Chemical Biology Symposium (1st ISSCB) in Chuncheon
  07 Feb. 2025, Oral presentation(invited, special)
• Multivalent affinity labeling probes for exploring carbohydrate-binding proteins.
  Asian Chemical Biology Initiative Hanoi Meeting,
  17 Jan. 2025, Oral presentation(invited, special)
• Multivalent affinity labeling probes for exploring carbohydrate-binding proteins.
  CCEB Chemistry Seminar Series,
  14 Aug. 2024, Oral presentation(invited, special)
• Multivalent Affinity Labeling Probes for Exploration of Carbohydrate-Binding Proteins.
  The 3rd Winter In-Person Organic Symposium (WIPOS) 2023
  21 Dec. 2023, Oral presentation(invited, special)
• Multivalent Affinity Labeling Probes for Exploration of Carbohydrate-Binding Proteins
  IUPAC the 13th International Symposium on Bioorganic Chemistry (ISBOC-13)
  18 Dec. 2023, Oral presentation(invited, special)
• 糖鎖結合タンパク質探索のための疎水性タグ修飾金ナノ粒子プローブの合成.
  2023 GlycoTOKYO　
  09 Dec. 2023, Poster presentation
• マルチバレント金ナノ粒子を活用した糖鎖結合タンパク質のアフィニティーラベリング法の開発.
  2023 GlycoTOKYO
  09 Dec. 2023, Oral presentation(invited, special)
• 抗がん活性天然物OSW-1の作用におけるオキシステロール結合タンパク質の役割.
  第46回日本分子生物学会年会　公募シンポジウム“脂質から迫るオルガネラダイナミクス”
  07 Dec. 2023, Oral presentation(invited, special)
• Target protein analysis of anticancer saponin OSW-1.
  The 28th French-Japanese Symposium on Medicinal and Fine Chemistry (FJS2023)
  02 Oct. 2023, Poster presentation
• The Role of Oxysterol-Binding Proteins in the Anticancer Activity of Steroidal Saponin OSW-1. Poster presentation
  The 28th French-Japanese Symposium on Medicinal and Fine Chemistry (FJS2023)
  02 Oct. 2023, Poster presentation
• Multivalent Affinity Labeling Probes for Exploration of Carbohydrate-binding Proteins
  The 28th French-Japanese Symposium on Medicinal and Fine Chemistry (FJS2023)
  02 Oct. 2023, Poster presentation
• Multivalent Affinity Labeling Probes for Exploration of Carbohydrate-binding Proteins.
  University of Rouen Normandy (COBRA) Departmental seminar
  29 Sep. 2023, Oral presentation(invited, special)
• Exploring Carbohydrate-Binding Proteins by Multivalent Affinity Labeling Probes
  6th Asian Chemical Biology Conference 2023 (ACBC2023)
  22 Aug. 2023, Oral presentation(invited, special)
• Analysis of binding proteins of yaku'amide B
  東京大学生命科学技術国際卓越大学院(WINGS-LST)全体会議
  24 Jun. 2023, Oral presentation(general)
• 異種の光反応基を修飾した金ナノ粒子プローブの反応解析.
  第84回有機合成化学協会関東支部シンポジウム
  13 May 2023, Poster presentation
• Investigation of Target Proteins of Anticancer Saponin OSW-1
  日本化学会第103 春季年会
  23 Mar. 2023, Oral presentation(general)
• タンパク質分解に向けた金ナノ粒子修飾マルチバレント疎水性タグの合成
  日本化学会第103 春季年会
  22 Mar. 2023, Poster presentation
• Binding interaction analysis of anticancer saponin OSW-1.
  Future Drug Discovery Empowered by Chemical Biology,
  22 Feb. 2023, Poster presentation
• Development of Multivalent Affinity Labeling Probes for Exploration of Carbohydrate-binding Proteins.
  Future Drug Discovery Empowered by Chemical Biology,
  22 Feb. 2023, Oral presentation(invited, special)
• The role of oxysterol-binding protein-related family proteins in the anticancer activity of steroidal saponin OSW-1
  第19回日本女性科学者の会学術大会
  04 Dec. 2022, Poster presentation
• Development of gold nanoparticle photoaffinity probes with different photoreactive groups
  第39回メディシナルケミストリーシンポジウム
  23 Nov. 2022, Poster presentation
• 糖鎖結合タンパク質探索に向けた金ナノ粒子ラベリングプローブの開発
  第19回日本糖鎖科学コンソーシアムシンポジウム
  22 Nov. 2022, Oral presentation(invited, special)
• 抗がん活性天然物OSW-1の作用におけるオキシステロール結合タンパク質の役割
  第16回 日本臨床ストレス応答学会大会
  05 Nov. 2022, Oral presentation(general)
• ジクロロトリアジン基をタンパク質反応性基とした金ナノ粒子プローブの開発
  第16 回バイオ関連化学シンポジウム
  12 Sep. 2022, Poster presentation
• 糖鎖結合タンパク質のアフィニティーラベリングに向けた金ナノ粒子プローブのラベル基探索
  第55回天然物化学談話会
  24 Jun. 2022, Poster presentation
• 金ナノ粒子フォトアフィニティープローブにおける異なる光反応基の反応性解析
  第55回天然物化学談話会
  24 Jun. 2022, Poster presentation
• Development of new electrophilic gold-nanoparticle probes for affinity labeling of target proteins.
  日本ケミカルバイオロジー学会第16回年会
  30 May 2022, Poster presentation
• 天然生物活性分子の標的タンパク質探索技術の開発と作用機構解明研究
  日本女性科学者の会　第27回 奨励賞・功労賞贈呈式
  22 May 2022, Oral presentation(invited, special)
• Design and synthesis of gold nanoparticle-based probes for affinity labeling carbohydrate-binding proteins
  PACIFICHEM2021, Symposium
  21 Dec. 2021, Poster presentation
• The Use of Affinity Probe for Binding Interaction Analysis of Anticancer Saponin OSW-1 and ORP Family Proteins,
  PACIFICHEM2021, Symposium
  20 Dec. 2021, Poster presentation
• Chemical probe-based approach for functional analysis of anticancer saponin OSW-1
  PACIFICHEM2021, Symposium
  19 Dec. 2021, Oral presentation(general)
• Multivalent approach to streamline affinity labeling of carbohydrate-binding proteins
  PACIFICHEM2021, Symposium
  18 Dec. 2021, Oral presentation(general)
• 糖鎖結合タンパク質のアフィニティーラベリングを目指した求電子性金ナノ粒子マルチバレントプローブの開発
  GlycoTOKYO2021シンポジウム
  08 Oct. 2021, Poster presentation
• 糖鎖結合タンパク質のアフィニティーラベリングに向けた求電子性金ナノ粒子マルチバレントプローブの開発
  第15 回バイオ関連化学シンポジウム
  08 Sep. 2021, Poster presentation
• Development of Gold Nanoparticle-Based Multivalent Photoaffinity Probes toward Exploration of Carbohydrate-Protein Interaction
  The 3rd Australasian Glycoscience Symposium
  03 Jun. 2021, Oral presentation(invited, special)
• Functional analysis of azide-functionalized gold nanoparticles　toward affinity labeling.
  日本化学会第101 回春季年会
  21 Mar. 2021, Poster presentation
• Evaluation of gold nanoparticle-based affinity labeling probes bearing electrophilic groups
  日本化学会第101 回春季年会
  21 Mar. 2021, Poster presentation
• Comparative study of multivalent electrophiles for affinity labeling of carbohydrate binding proteins.
  JSPS A3 Foresight Program, Asian Chemical Probe Research Hub, A3 Young Scientists’ Meeting
  22 Feb. 2021, Oral presentation(invited, special)
• Multivalent affinity labeling probes for target protein analysis
  Departmental seminar
  04 Dec. 2020, Oral presentation(invited, special)
• Evaluation of electrophilic groups for gold nanoparticle-based affinity labeling probes
  日本化学会第100回春季年会
  24 Mar. 2020, Poster presentation
• 糖鎖結合タンパク質探索に向けた金ナノ粒子アフィニティーラベリングプローブの開発
  日本化学会第100回春季年会
  23 Mar. 2020, Oral presentation(general)
• 金ナノ粒子を基盤としたclickableフォトアフィニティープローブの開発
  日本化学会第100回春季年会 2020年
  23 Mar. 2020, Oral presentation(general)
• Target Identificaiton
  Asian Chemical Biology Initiative Jakarta Meeting
  07 Dec. 2019, Public discourse, seminar, tutorial, course, lecture and others
• Development of gold nanoparticle-based clickable photoaffinity probes for target protein identification
  2nd Australasian Glycoscience Symposium-18th World HUPO Congress
  17 Sep. 2019, Poster presentation
• Development of gold nanoparticle-based affinity labeling probes for analyzing carbohydrate-binding proteins
  2nd Australasian Glycoscience Symposium-18th World HUPO Congress
  17 Sep. 2019, Poster presentation
• Development of gold nanoparticle-based multivalent photoaffinity probes toward exploration of carbohydrate-protein interaction
  2nd Australasian Glycoscience Symposium-18th World HUPO Congress
  16 Sep. 2019, Oral presentation(general)
• 金ナノ粒子アフィニティーラベリングプローブの開発
  H30年度 TIAナノバイオスクール(糖鎖・レクチン）
  04 Sep. 2019, Symposium workshop panel(public)
• 糖鎖結合タンパク質探索のための金ナノ粒子プローブ開発
  H30年度 TIAナノバイオスクール(糖鎖・レクチン）
  04 Sep. 2019, Symposium workshop panel(public)
• Development of Gold Nanoparticle-Based Affinity Labeling Probes for Target Protein Analysis
  第14回ケミカルバイオロジー学会年会
  12 Jun. 2019, Poster presentation
• Development of gold nanoparticle-based clickable photoaffinity labeling agent for target protein identification
  第14回ケミカルバイオロジー学会年会
  11 Jun. 2019, Poster presentation
• 金ナノ粒子を基盤としたアフィニティ-ラベリングプローブの開発
  第18回新規素材探索研究会セミナー
  07 Jun. 2019, Poster presentation
• Development of Small Molecule-Conjugated Gold Nanoparticle Probes for Target Protein Analysis
  Departmental Seminar
  19 Apr. 2019, Oral presentation(invited, special)
• Development of Small Molecule-Conjugated Gold Nanoparticle Probes for Target Protein Analysis
  Departmental Seminar
  17 Apr. 2019, Oral presentation(invited, special)
• Development of Gold-Nanoparticle Based Probes for Target Identification and Analysis of Bioactive Small-Molecules
  Departmental Seminar
  20 Mar. 2019, Oral presentation(invited, special)
• Development of Gold Nanoparticle-Based　Affinity Labeling Probes for　Target Protein Analysis
  第99回日本化学会春季年会
  18 Mar. 2019, Poster presentation
• Development of Gold Nanoparticle-Based Click-Photocapture Agents for Target Protein Identification
  会第99回日本化学会春季年会
  16 Mar. 2019, Poster presentation
• Development of Gold-Nanoparticle Based Probes for Target Identification and Analysis of Bioactive Small Molecules
  Asian Chemical Biology Initiative Yangong Meeting
  20 Jan. 2019, Oral presentation(invited, special)
• Development of gold-nanoparticle based probes for target identification and analysis of bioactive small molecules
  Singapore International Chemistry Conference (SICC-10)
  18 Dec. 2018, Oral presentation(invited, special)
• 抗癌活性サポニンOSW-1の化学プローブを用いたOSBPパラログ選択的結合活性の解析
  GlycoTOKYO2018シンポジウム
  10 Dec. 2018, Poster presentation
• Development of Gold-Nanoparticle Based Probes for Target Identification and Analysis of Bioactive Small Molecules
  A3 Foresight Program Round Table Meeting on Chemical Probe Research Hub
  02 Nov. 2018, Oral presentation(general)
• Chemical-Probe Based Approach to Binding Interaction Analysis of Anticancer Natural Product OSW-1
  The 2nd A3 Young Scientist Meeting
  20 Jun. 2018, Oral presentation(invited, special)
• 生物活性分子の作用機構解明に向けた標的タンパク質探索法
  日本化学会 第98春季年会
  20 Mar. 2018, Oral presentation(invited, special)
• 生物活性分子の作用機構解明に向けた標的タンパク質探索法
  Asian Chemical Probe Research Hub Symposium天然物有機化学・天然物合成とケミカルバイオロジー
  13 Feb. 2018, Oral presentation(invited, special)
• Chemical Probe-Based Approach to Study Bioactivity of Natural Products
  Departmental Seminar
  24 Jan. 2018, Oral presentation(invited, special)
• Chemical Probe-Based Approach to Study Bioactivity of Natural Products
  Departmental Seminar
  22 Jan. 2018, Oral presentation(invited, special)
• 活性部位に光反応基を導入したOSW-1フォトアフィニティープローブの開発
  SJWS2018新春学術シンポジウム
  07 Jan. 2018, Poster presentation
• タンパク質探索に向けたa-Tomatineフォトアフィニティープローブの開発
  第７回CSJ化学フェスタ
  19 Oct. 2017, Poster presentation
• 金ナノ粒子プローブを用いた低分子結合タンパク質の新規ラベル化技術の開発
  第７回CSJ化学フェスタ
  19 Oct. 2017, Poster presentation
• OSW-1結合たんぱく質探索に向けたactive/inactive 二種photoaffinity probeの開発
  第７回CSJ化学フェスタ
  19 Oct. 2017, Poster presentation
• Development of OSW-1 photoaffinity probes by sequential site-selective acylation
  26th French-Japanese Symposium on Medicinal & Fine Chemistry
  18 Sep. 2017, Poster presentation
• Chemical Probe-Based Approach to Study Bioactivity of Natural Product” Sakurai. K., Asian Chemical Biology Initiative
  Asian Chemical Biology Initiative Ulaanbaataar Meeting
  02 Sep. 2017, Oral presentation(invited, special)
• 活性部位に光反応基を導入したOSW-1フォトアフィニティープローブの合成
  第51回天然物化学談話会
  06 Jul. 2017, Poster presentation
• リンカー長が異なるビオチン化OSW-1プローブを用いた結合タンパク質探索
  第51回天然物化学談話会
  06 Jul. 2017, Poster presentation
• 生物活性分子の作用機序解明に向けた標的タンパク質探索法の開発
  新規素材探索研究会第16回セミナー
  09 Jun. 2017, Oral presentation(invited, special)
• Development of novel OSW-1 photoaffinity probes bearing a photoreactive group at its putative protein binding site
  第11回ケミカルバイオロジー学会年会
  07 Jun. 2017, Poster presentation
• Synthesis of biotinylated OSW-1 with varied linker length and their functional analysis
  第11回ケミカルバイオロジー学会年会
  07 Jun. 2017, Poster presentation
• Development of Chemical Probes for Studying the Anticancer Activity of　OSW-1
  Spanish-Japanese Symposium on Modern Synthetic Methodology
  24 Apr. 2017, Oral presentation(invited, special)
• Development of a biotinylated photoaffinity probe based on an antitumor saponin OSW-1.
  日本化学会 第96春季年会
  17 Mar. 2017, Poster presentation
• Development of gold nanoparticle-based photoaffinity probes displaying bioactive small molecule ligands.
  日本化学会 第96春季年会
  16 Mar. 2017, Other
• Use of chemical probes of an antitumor saponin OSW-1 for analysis of its binding proteins.
  日本化学会 第96春季年会
  16 Mar. 2017, Other
• Development of Chemical Probes for Studying the Anticancer Activity of OSW-1
  Asian Chemical Biology Initiative Ho Chi Minh Meeting
  21 Jan. 2017, Oral presentation(invited, special)
• 結合タンパク質探索に向けた糖脂質フォトアフィニティープローブの開発
  GlycoTOKYO2016
  19 Nov. 2016, Poster presentation
• Development of chemical probes for studying the antitumor activity of OSW-1
  Departmental Seminar
  26 Sep. 2016, Oral presentation(invited, special)
• リンカー長が異なるビオチン化OSW-1プローブの開発
  第50回天然物化学談話会
  06 Jul. 2016, Poster presentation
• 天然物標的タンパク質の効率的探索に向けた金ナノ粒子フォトアフィニティープローブの開発
  第50回天然物化学談話会
  06 Jul. 2016, Poster presentation
• 抗癌活性サポニンOSW-1の作用機序解明に向けた標的タンパク質探索」
  日本学術振興会第189委員会「日本におけるケミカルバイオロジーの新展開」　平成28年度第1回定例会、
  10 Jun. 2016, Oral presentation(general)
• Development of chemical probes for studying the antitumor activity of OSW-1
  Departmental Seminar
  25 May 2016, Oral presentation(invited, special)
• Development of chemical probes for studying the antitumor activity of OSW-1
  Departmental Seminar
  24 May 2016, Oral presentation(invited, special)
• Development of photoaffinity probe based on an inactive analog of an antitumor saponin OSW-1
  日本化学会 第95春季年会
  26 Mar. 2016, Poster presentation
• Development of photoaffinity probes based on gold nanoparticles for exploration of small molecule binding proteins
  日本化学会 第95春季年会
  26 Mar. 2016, Poster presentation
• 抗癌活性サポニンOSW-1の三次元構造と化学プローブを用いた作用解析
  第27回ERATOタンパク質関連情報交換会
  20 Jan. 2016, Oral presentation(invited, special)
• Development of chemical probes for studying mechanism of action of an antitumor saponin OSW-1
  PACIFICHEM 2015
  16 Dec. 2015, Oral presentation(general)
• Design and synthesis of a photoreactive OSW-1 analog and its application to a photoaffinity labeling study
  PACIFICHEM 2015
  16 Dec. 2015, Poster presentation
• Synthesis of a biotinylated OSW-1 probe and its application to the investigation of binding proteins
  第33回メディシナルケミストリーシンポジウム
  25 Nov. 2015, Poster presentation
• Development of dual-probe photoaffinity labeling for searching carbohydrate binding proteins.
  第33回メディシナルケミストリーシンポジウム
  25 Nov. 2015, Poster presentation
• Synthesis of OSW-1 Photoaffinity Probe and Its Biological Evaluation
  第57回天然有機化合物討論会
  09 Sep. 2015, Poster presentation
• Development of　multivalent　carbohydrate photoaffinity　probes based on gold nanoparticles
  第34回日本糖質学会年会
  28 Jun. 2015, Other
• Development of Sterol-based Affinity Probes for Exploration of Target Proteins
  第9回ケミカルバイオロジー学会年会
  12 Jun. 2015, Poster presentation
• Development of a photoaffinity probe of anti-cancer natural product OSW-1
  第9回ケミカルバイオロジー学会年会
  12 Jun. 2015, Poster presentation
• OSW-1類縁体2”位に光反応基を導入した蛍光フォトアフィニティプローブの合成
  第71回有機合成化学協会関東支部シンポジウム
  14 May 2015, Other
• Development of diazirine-based carbohydrate probes for dual-probe photoaffinity labeling
  日本化学会 第94春季年会
  27 Mar. 2015, Poster presentation
• Development of a photoaffinity probe based on an anticancer saponin OSW-1
  日本化学会 第94春季年会
  27 Mar. 2015, Other
• Development of gold nanoparticle-based multivalent carbohydrate probes for selective photoaffinity labeling and enrichment of carbohydrate-binding proteins
  日本化学会 第94春季年会
  26 Mar. 2015, Poster presentation
• Application of dual-probe photoaffinity labeling toward exploration of carbohydrate binding proteins.
  日本化学会 第94春季年会
  26 Mar. 2015, Other
• Synthesis and Biological Evaluation of OSW-1 Analogs as Chemical Probes
  8th Singapore International Chemistry Conference
  15 Dec. 2014, Oral presentation(invited, special)
• The synthesis and functional analysis of glycolipid photoaffinity probes
  第４回　CSJ化学フェスタ
  16 Oct. 2014, Poster presentation
• 位置選択的なモノアシル化反応を用いたOSW-1のビオチンプローブの合成と 生物活性評価
  第４回　CSJ化学フェスタ
  15 Oct. 2014, Poster presentation
• 糖脂質結合タンパク質探索にむけた フォトアフィニティープローブの合成と機能解析
  第49回天然物化学談話会
  03 Jul. 2014, Poster presentation
• 位置選択的なモノアシル化反応を用いたOSW-1の分子プローブの合成と 生物活性評価
  第67回有機合成化学協会関東支部シンポジウム
  17 May 2014, Other
• Synthesis of Fluorescently Labeled Analog of Antitumor Saponin OSW-1
  日本化学会 第94春季年会
  27 Mar. 2014, Poster presentation
• Development of glycolipid nanoprobes for efficient enrichment of carbohydrate binding proteins
  日本化学会 第94春季年会
  27 Mar. 2014, Poster presentation
• Dual-probe photoaffinity labeling strategy for selective detection of small-molecule binding proteins
  10th Japanese-German Frontiers of Science Symposium
  01 Nov. 2013, Poster presentation
• フォトアフィニティーラベリングを用いた低分子―タンパク質相互作用解析
  第１３回有機合成化学協会関東支部談話会
  27 Sep. 2013, Oral presentation(invited, special)
• Fluorescently Labeled OSW-1 and Its Biological Evaluation
  第48回天然物化学談話会
  05 Jul. 2013, Poster presentation
• Development of a Solid-Phase Photoaffinity Labeling Method Based on Glycolipid Gold Nanoparticles
  第48回天然物化学談話会
  05 Jul. 2013, Poster presentation
• Fluorescently Labeled OSW-1 and Its Biological Evaluation
  第8回ケミカルバイオロジー学会年会
  19 Jun. 2013, Poster presentation
• Dual-Photoaffinity Labeling System for Exploring Glycolipid Binding Proteins
  日本化学会 第93春季年会
  22 Mar. 2013, Poster presentation
• Development of a Dual Affinity Probe System for the Detection of the Carbohydrate Binding Proteins Using Glycolipid-Gold Nanoparticles
  日本化学会 第93春季年会
  22 Mar. 2013, Poster presentation
• Comparative Analysis on the Reactivity of Photoreactive Groups for Photoaffinity Labeling of the Carbohydrate Binding Proteins
  日本化学会 第93春季年会
  22 Mar. 2013, Poster presentation
• Development of a Solid-Phase Photoaffinity Labeling Method Based on Glycolipid-goldnanoparticles
  日本化学会 第93春季年会
  22 Mar. 2013, Other
• 抗癌活性サポニンOSW-1の3次元構造と分子認識
  新学術領域研究「天然物ケミカルバイオロジー～分子標的と活性制御～」地区ミニシンポジウム
  23 Feb. 2013, Oral presentation(invited, special)
• Development of Dual-Probe Photoaffinity Labeling System for Selective Detection of Small-Molecule Binding Proteins
  第2回CSJ化学フェスタ2012
  16 Oct. 2012, Poster presentation
• 低分子-タンパク質間相互作用を探る Chemical Biology研究
  早稲田大学先進理工学部　化学・生命化学科 実践的化学知セミナー
  13 Jul. 2012, Oral presentation(invited, special)
• Development of a Selective Photoaffinity Labelling Approach for Probing Protein-Ligand Interaction
  The 12th HFSP Awardees Meeting
  03 Jul. 2012, Poster presentation
• Development of a Selective Photoaffinity Labelling Approach for Probing Protein-Ligand Interaction
  天然物ケミカルバイオロジー　～分子標的と活性制御～　第2回シンポジウム
  17 Jun. 2012, Poster presentation
• Development of a new photoaffinity labeling method using two kinds of probe.
  日本化学会第92春季年会
  25 Mar. 2012, Poster presentation
• Development of Photoaffinity Labeling Method for Analysis of Small Molecule – Protein Interactions
  日本化学会関東支部大会
  31 Aug. 2011, Poster presentation
• Dual-Probe Approach to Photoaffinity Labeling of Small-Molecule Binding Proteins
  The 6th Korea-Japan Young Scientists Meeting on Bioorganic and Natural Products Chemistry
  23 Jun. 2011, Poster presentation
• “Three-Dimensional Structure of Antitumor Glycosylsterols from Ornithogalum saundersiae”
  The 6th Korea-Japan Young Scientists Meeting on Bioorganic and Natural Products Chemistry
  23 Jun. 2011, Oral presentation(invited, special)
• Development of Photoaffinity Probes aimed at Establishment of a Target Protein Labeling Method
  日本ケミカルバイオロジー学会第6会年会
  25 May 2011, Poster presentation
• Development of a target protein labeling method using a photoaffinity probe
  日本化学会第91回春季年会
  26 Mar. 2011, Poster presentation
• Synthesis of Fluorescently Labelled Analogs of Antitumor Saponin OSW-1
  The International Chemical Congress of Pacific Basin Societeis
  18 Dec. 2010, Poster presentation
• Structure Analysis of A Steroidal Glycoside from Ornithogalum Saundersiae
  The International Chemical Congress of Pacific Basin Societeis
  18 Dec. 2010, Poster presentation
• Conformational Studies of Highly Potent Antitumor Steroidal Glycosides from Ornithogalum saundersiae.
  The International Chemical Congress of Pacific Basin Societeis
  17 Dec. 2010, Oral presentation(general)
• On the molecular rational for the biological activity of a steroidal glycoside from Ornithogalum saundersiae
  日本化学会第90春季年会
  28 Mar. 2010, Other
• Development of Metabolically Stable a-Helical Peptide Inhibitors for Targeting p53-MDM2 Interface
  The Mona Symposium, Natural Products and Medicinal Chemistry, the 22nd Meeting,
  07 Jan. 2008, Other
• Discovering Functional Small Molecules through DNA-Templated Organic Synthesis (DTS) and In Vitro Selection Methods
  日本ケミカルバイオロジー研究会　第2回年会
  May 2007, Other
• Two Chemical Approaches Ｔoward Targeting Protein Surfaces
  理化学研究所 ケミカルバイオロジー研究セミナー
  Apr. 2007, Oral presentation(invited, special)
• Application of DNA-Templated Synthesis and In Vitro Selection for Discovery of Functional Small Molecules
  第48回天然有機化合物討論会
  11 Oct. 2006, Oral presentation(general)
• Two Chemical Approaches Toward Targeting Protein Surfaces
  第４１回天然物化学談話会
  04 Jul. 2006, Oral presentation(invited, special)

Committee Memberships

• 有機合成化学協会
  「ニューモダリティと有機合成化学」研究部会幹事委員
  From 20240101, To 20250331
• 日本化学会
  日本化学会 産学交流委員会CIP企画小委員会委員
  From 20210501, To 20260430
• 日本ケミカルバイオロジー学会
  産学連携委員会（ケミカルバイオロジーの技術革新）幹事委員(会計監査)
  From 20201001, To 20250331
• 日本化学会
  日本化学会 産学交流委員会ATP企画小委員会委員
  From 20150522, To 20210430

Media Coverage

• Dysregulation of PI4P in the trans Golgi regions activates the mammalian Golgi stress response.
  Nature Index Article
  From 13 Dec. 2024, To 13 Dec. 2024
• 東京農工大、抗がん活性化合物OSW-1のビオチン化プローブではリンカーの長さが標的タンパク質を見つける鍵であることを解明
  農工大　生命機能科学部門の櫻井香里教授らの研究グループは、強力な抗がん作用をもつOSW-1の標的タンパク質を探索するために最適化されたビオチン化プローブを開発した、と掲載される。
  日経バイオテク
  From 08 Jan. 2024, To 08 Jan. 2024
• 奨励賞に東京農工大の櫻井准教授ら　日本女性科学者の会
  「日本女性科学者の会」が、第２７回奨励賞に東京農工大大学院の櫻井香里准教授ら３人を選出したことが紹介される。
  産経新聞／大学ジャーナル
  From 24 Apr. 2022, To 25 Apr. 2022
• 東京農工大、求電子性金ナノ粒子アフィニティーラベリングプローブを開発
  日経新聞電子版
  From 24 Nov. 2021
• Researchers use multivalent gold nanoparticles to develop efficient molecular probe
  Kaori Sakurai, associate professor at Tokyo University Agriculture and Technology use multivalent gold nanoparticles to develop efficient molecular probe.
  PHYS ORG
  From 07 Jul. 2021, To 07 Jul. 2021
• New gold-nanoparticle probes for target identification of bioactive small molecules
  Researchers at Tokyo University of Agriculture and Technology (TUAT) have developed a new, streamlined method to better understand the molecular mechanisms underpinning these interactions.
  NEWS Medical Life Sciences
  From 01 Feb. 2021, To 01 Feb. 2021
• Easily assembled gold nanoparticle scaffolding serves as molecular probe
  Researchers at Tokyo University of Agriculture and Technology (TUAT) have developed a new, streamlined method to better understand the molecular mechanisms underpinning these interactions.
  Phys.org
  From 01 Feb. 2021, To 01 Feb. 2021

Professional Memberships

• Japanese Chemical Biology Society
  From 2007
• 新規素材探索研究会
• 東京糖鎖研究会
• The Human Proteome Organization
• 有機合成化学協会
• 日本薬学会
• 日本糖質学会
• 日本化学会
• American Chemical Society

Awards

• 日本女性科学者の会
  第27回（2022年度）日本女性科学者の会奨励賞
  天然生物活性分子の標的タンパク質探索技術の開発と作用機構解明研究
  広く理系の分野において研究業績をあげ、その将来性を期待できる方で、かつ日本女性科学者の会の趣旨に賛同し、その達成のために努力していると認められる日本女性科学者の会正会員および学生会員を対象とする。　日本女性科学者の会について：一般社団法人日本女性科学者の会は、1996年に設立され、女性科学者の友好を深め、研究分野の知識の交換を図り、その地位向上を目指すとともに、女性と男性がともに個性と能力を発揮できる環境とネットワークの構築を推進することで、社会と世界の平和に貢献することを目的として活動している。
  15 Apr. 2022
• Southern University of Science and Technology (SUSTech)
  The 146th SUSTECH Chemical Sciences Lectureship Award
  Development of Small Molecule-Conjugated Gold Nanoparticle Probes for Target Protein Analysis
  Apr. 2019
• International Human Frontier Science Program Organization
  Human Frontier Science Program Young Investigator Grant Award
  Probing the role of lipids in cell division
  Aurelien Roux, Ulrike Eggertらと共同受賞
  2009
• 長瀬科学技術振興財団
  平成19年度長瀬科学技術振興財団研究振興賞
  Apr. 2007
• 天然物化学談話会
  第五回天然物化学談話会奨励賞受賞
  Two Chemical Approaches Toward Targeting MDM2
  Aug. 2005