Researcher Database
HARIMA Hayato
| ■Faculty | Institute of Agriculture Division of Animal Life Science | |||
| ■Position | Assistant Professor | |||
Last Updated :2026/07/29
Activity information
Name and contact details
Name
ハリマ ハヤト, 播磨 勇人, HARIMA Hayato
Affiliation / Position
Institute of Agriculture Division of Animal Life Science, Assistant Professor
Other affiliation
Research History
- 東京農工大学
農学研究院
助教
From 01 Apr. 2022 - Hokakido University
International Institute for Zoonosis Control
Post-Doctoral Fellow
From 01 Apr. 2017, To 31 Mar. 2022
Education
- 東京大学大学院
医学系研究科
病因・病理学専攻
To 31 Mar. 2017, completed, doctor course - Azabu University
School of Veterinary Medicine
Department of Veterinary Medicine
To 31 Mar. 2013, graduated
Degree
Licenses and qualifications
Current state of research and teaching activities
Research Areas
Research Interests
Grants-in-Aid for Scientific Research
- 若手研究
コウモリ由来オルソレオウイルスの野生株に基づく抗原性比較解析
From 2024, To 2025 - 若手研究
野生株間比較によるコウモリ由来オルソレオウイルスの病原性発現機構の解明
From 2021, To 2023
Papers
- Mosquito-borne alphaviruses in Zambia: Isolation and characterization of Eilat and Sindbis viruses.
Virus Res
Aug. 2025, Research paper (scientific journal), DOI(公開)(r-map) - Impact of the changes in substrate specificity of herpes simplex virus 1 protein kinase Us3 on viral infection in vitro and in vivo.
J Virol
22 Jul. 2025, Research paper (scientific journal), DOI(公開)(r-map) - Herpes simplex virus 1 evades APOBEC1-mediated immunity via its uracil-DNA glycosylase in mice.
Nat Microbiol
Jul. 2025, Research paper (scientific journal), DOI(公開)(r-map) - Determination of Antimicrobial Resistance Megaplasmid-Like pESI Structures Contributing to the Spread of Salmonella Schwarzengrund in Japan.
Ishihara, Kanako; Someno, Suzuka; Matsui, Kaoru; Nakazawa, Chisato; Abe, Takahiro; Harima, Hayato; Omatsu, Tsutomu; Ozawa, Manao; Iwabuchi, Eriko; Asai, Tetsuo
Antibiotics (Basel)
MDPI
Background/Objectives: The acquisition of antimicrobial resistance by foodborne pathogens is a serious human health concern. In Japan, combinations of antimicrobial resistance genes in Salmonella from chicken meat were common among several serovars. Therefore, we hypothesized that different S. enterica serovars share a common antimicrobial resistance plasmid. Methods: Antimicrobial resistance transfer was tested in S. Infantis and S. Schwarzengrund, the major serovars used as donors. The plasmid structure was determined by subjecting S. Infantis Sal_238 and S. Schwarzengrund Sal_249 to short- and long-read sequencing. Results: The high homology between pSal_249Sch and pSal_238Inf suggests they have a common ancestor. Because the sequences of pSal_238Inf and pSal_249Sch were highly homologous to pESI (a plasmid for emerging S. Infantis), pSal_238Inf and pSal_249Sch were identified as pESI-like plasmids. S. Schwarzengrund is the third Salmonella serovar to expand its distribution related to pESI-like plasmid acquisition. Core-genome multilocus sequence-type analysis revealed that S. Schwarzengrund isolates with pESI-like plasmids from Japan (core-genome sequence-type [cgST] 167363 and cgST287831), the UK (cgST167363), and the USA (cgST167363, cgST196045, and cgST287831) were closely related; they are also suggested to share a common ancestor. The transfer of antimicrobial resistance was observed in combinations of both serovars. Specifically, the tentative plasmid sequence obtained via short-read sequencing, PCR, and conjugation experiments identified deletions of antimicrobial resistance genes (aadA, sul1, and tetA), class 1 integron, mercury resistance operon, and/or plasmid transfer region in the pESI-like plasmid. Conclusion: These data on the structural diversity of pESI-like plasmids suggest that some time has passed since S. Schwarzengrund acquired them.
10 Mar. 2025, Research paper (scientific journal), joint, 14, 3, 2079-6382, DOI(公開)(r-map) - First identification and whole genome characterization of rotavirus C in pigs in Zambia
Virology
Feb. 2025, Research paper (scientific journal), DOI(公開)(r-map) - A first report of rotavirus B from Zambian pigs leading to the discovery of a novel VP4 genotype P[9]
Virol J
24 Oct. 2024, Research paper (scientific journal), DOI(公開)(r-map) - Characterization of a mammalian orthoreovirus isolated from the large flying fox, Pteropus vampyrus, in Indonesia.
J Gen Virol
2024, Research paper (scientific journal), DOI(公開)(r-map) - Meningoencephalitis with malacia caused by Sarcocystis calchasi in a rock pigeon in Japan
J Vet Med Sci
Aug. 2024, Research paper (scientific journal), DOI(公開)(r-map) - Expanding diversity of bunyaviruses identified in mosquitoes
Sci Rep
24 Oct. 2023, Research paper (scientific journal), DOI(公開)(r-map) - Prevalence and Genomic Characterization of Rotavirus A from Domestic Pigs in Zambia: Evidence for Possible Porcine-Human Interspecies Transmission
Ndebe, Joseph; Harima, Hayato; Chambaro, Herman Moses; Sasaki, Michihito; Yamagishi, Junya; Kalonda, Annie; Shawa, Misheck; Qiu, Yongjin; Kajihara, Masahiro; Takada, Ayato; Sawa, Hirofumi; Saasa, Ngonda; Simulundu, Edgar; Bastos, Armanda; Tchouassi, David P.
PATHOGENS
MDPI
Rotavirus is a major cause of diarrhea globally in animals and young children under 5 years old. Here, molecular detection and genetic characterization of porcine rotavirus in smallholder and commercial pig farms in the Lusaka Province of Zambia were conducted. Screening of 148 stool samples by RT-PCR targeting the VP6 gene revealed a prevalence of 22.9% (34/148). Further testing of VP6-positive samples with VP7-specific primers produced 12 positives, which were then Sanger-sequenced. BLASTn of the VP7 positives showed sequence similarity to porcine and human rotavirus strains with identities ranging from 87.5% to 97.1%. By next-generation sequencing, the full-length genetic constellation of the representative strains RVA/pig-wt/ZMB/LSK0137 and RVA/pig-wt/ZMB/LSK0147 were determined. Genotyping of these strains revealed a known Wa-like genetic backbone, and their genetic constellations were G4-P[6]-I5-R1-C1-M1-A8-N1-T1-E1-H1 and G9-P[13]-I5-R1-C1-M1-A8-N1-T1-E1-H1, respectively. Phylogenetic analysis revealed that these two viruses might have their ancestral origin from pigs, though some of their gene segments were related to human strains. The study shows evidence of reassortment and possible interspecies transmission between pigs and humans in Zambia. Therefore, the One Health surveillance approach for rotavirus A in animals and humans is recommended to inform the design of effective control measures.
Oct. 2023, Research paper (scientific journal), joint, 12, 10, DOI(公開)(r-map) - Role of multidrug resistance and co-resistance on a high percentage of streptomycin resistance in Escherichia coli isolated from chicken meats in Japan
Lin, Thein; Nomura, Shizuka; Someno, Suzuka; Abe, Takahiro; Nishiyama, Miyuki; Shiki, Shunya; Harima, Hayato; Ishihara, Kanako
JOURNAL OF VETERINARY MEDICAL SCIENCE
JAPAN SOC VET SCI
We focused on streptomycin resistance because of the high percentage of streptomycin-resistant Escherichia coli concerning the amount used of streptomycin. Antimicrobial resistance and horizontal transfer were identified in 117 isolates of coliform bacteria from chicken meat to identify the factors that increase streptomycin resistance. Escherichia (45 isolates) was the predominant genus. Most streptomycin-resistant Escherichia isolates were resistant to other antimicrobials (17/18), suggesting that using various antimicrobials could select streptomycin -resistant Escherichia isolates. Resistance was transferred from 7 out of the 18 streptomycin-resistant isolates. The transconjugants acquired strA/strB (7/7), blaTEM (5/7), aphA1 (5/7), tetB (3/7), dfrA14 (1/7) and/or dfrA17 (1/7). The co-resistance of streptomycin resistance with other resistances would also increase streptomycin resistance.
Aug. 2023, Research paper (scientific journal), joint, 85, 8, 0916-7250, DOI(公開)(r-map), 832, 836 - Surveillance, Isolation, and Genetic Characterization of Bat Herpesviruses in Zambia
Harima, Hayato; Qiu, Yongjin; Yamagishi, Junya; Kajihara, Masahiro; Changula, Katendi; Okuya, Kosuke; Isono, Mao; Yamaguchi, Tomoyuki; Ogawa, Hirohito; Nao, Naganori; Sasaki, Michihito; Simulundu, Edgar; Mweene, Aaron S.; Sawa, Hirofumi; Ishihara, Kanako; Hang'ombe, Bernard M.; Takada, Ayato
VIRUSES-BASEL
MDPI
Bats are of significant interest as reservoirs for various zoonotic viruses with high diversity. During the past two decades, many herpesviruses have been identified in various bats worldwide by genetic approaches, whereas there have been few reports on the isolation of infectious herpesviruses. Herein, we report the prevalence of herpesvirus infection of bats captured in Zambia and genetic characterization of novel gammaherpesviruses isolated from striped leaf-nosed bats (Macronycteris vittatus). By our PCR screening, herpesvirus DNA polymerase (DPOL) genes were detected in 29.2% (7/24) of Egyptian fruit bats (Rousettus aegyptiacus), 78.1% (82/105) of Macronycteris vittatus, and one Sundevall's roundleaf bat (Hipposideros caffer) in Zambia. Phylogenetic analyses of the detected partial DPOL genes revealed that the Zambian bat herpesviruses were divided into seven betaherpesvirus groups and five gammaherpesvirus groups. Two infectious strains of a novel gammaherpesvirus, tentatively named Macronycteris gammaherpesvirus 1 (MaGHV1), were successfully isolated from Macronycteris vittatus bats, and their complete genomes were sequenced. The genome of MaGHV1 encoded 79 open reading frames, and phylogenic analyses of the DNA polymerase and glycoprotein B demonstrated that MaGHV1 formed an independent lineage sharing a common origin with other bat-derived gammaherpesviruses. Our findings provide new information regarding the genetic diversity of herpesviruses maintained in African bats.
Jun. 2023, Research paper (scientific journal), joint, 15, 6, DOI(公開)(r-map) - Isolation and Characterization of Distinct Rotavirus A in Bat and Rodent Hosts
Kishimoto, Mai; Kajihara, Masahiro; Tabata, Koshiro; Itakura, Yukari; Toba, Shinsuke; Ozono, Seiya; Sato, Yuko; Suzuki, Tadaki; Ito, Naoto; Changula, Katendi; Qiu, Yongjin; Mori-Kajihara, Akina; Eto, Yoshiki; Harima, Hayato; Mwizabi, Daniel; Hang'ombe, Bernard M. M.; Hall, William W. W.; Takada, Ayato; Orba, Yasuko; Sawa, Hirofumi; Sasaki, Michihito
JOURNAL OF VIROLOGY
AMER SOC MICROBIOLOGY
Recent advances in nucleotide sequence detection methods have enabled the detection of RVA genomes from various animals. These studies have discovered multiple divergent RVAs and have resulted in proposals for the genetic classification of novel genotypes. Rotavirus A (RVA) causes diarrheal disease in humans and various animals. Recent studies have identified bat and rodent RVAs with evidence of zoonotic transmission and genome reassortment. However, the virological properties of bat and rodent RVAs with currently identified genotypes still need to be better clarified. Here, we performed virus isolation-based screening for RVA in animal specimens and isolated RVAs (representative strains: 16-06 and MpR12) from Egyptian fruit bat and Natal multimammate mouse collected in Zambia. Whole-genome sequencing and phylogenetic analysis revealed that the genotypes of bat RVA 16-06 were identical to that of RVA BATp39 strain from the Kenyan fruit bat, which has not yet been characterized. Moreover, all segments of rodent RVA MpR12 were highly divergent and assigned to novel genotypes, but RVA MpR12 was phylogenetically closer to bat RVAs than to other rodent RVAs, indicating a unique evolutionary history. We further investigated the virological properties of the isolated RVAs. In brief, we found that 16-06 entered cells by binding to sialic acids on the cell surface, while MpR12 entered in a sialic acid-independent manner. Experimental inoculation of suckling mice with 16-06 and MpR12 revealed that these RVAs are causative agents of diarrhea. Moreover, 16-06 and MpR12 demonstrated an ability to infect and replicate in a 3D-reconstructed primary human intestinal epithelium with comparable efficiency to the human RVA. Taken together, our results detail the unique genetic and virological features of bat and rodent RVAs and demonstrate the need for further investigation of their zoonotic potential.IMPORTANCE Recent advances in nucleotide sequence detection methods have enabled the detection of RVA genomes from various animals. These studies have discovered multiple divergent RVAs and have resulted in proposals for the genetic classification of novel genotypes. However, most of these RVAs have been identified via dsRNA viral genomes and not from infectious viruses, and their virological properties, such as cell/host tropisms, transmissibility, and pathogenicity, are unclear and remain to be clarified. Here, we successfully isolated RVAs with novel genome constellations from three bats and one rodent in Zambia. In addition to whole-genome sequencing, the isolated RVAs were characterized by glycan-binding affinity, pathogenicity in mice, and infectivity to the human gut using a 3D culture of primary intestinal epithelium. Our study reveals the first virological properties of bat and rodent RVAs with high genetic diversity and unique evolutional history and provides basic knowledge to begin estimating the potential of zoonotic transmission.
Jan. 2023, Research paper (scientific journal), joint, 97, 1, 0022-538X, DOI(公開)(r-map)