Researcher Database

MATSUSHITA Yasuhiko

FacultyResearch Center for Science and Technology Gene Research Center
PositionAssociate Professor
Last Updated :2024/01/26

Activity information

Name and contact details

  • Name

    マツシタ ヤスヒコ, 松下 保彦, MATSUSHITA Yasuhiko
  • E-mail

    ymatsucc.tuat.ac.jp

Affiliation / Position

  • Research Center for Science and Technology Gene Research Center, Associate Professor

Other affiliation

  • Research Center for Science and Technology Gene Research Center
  • Graduate School of Agriculture Department of Applied Biological Chemistry
  • Faculty of Agriculture Department of Applied Biological Science

Research History

  •  
    日本学術振興会特別研究員(神戸大学)
    From Apr. 1991, To Mar. 1993
  •  
    英国ロンドン大学キングスカレッジ博士研究員
    From Apr. 1993, To Mar. 1996
  •  
    東京農工大学助手遺伝子実験施設
    From Apr. 1996, To Mar. 2002
  •  
    東京農工大学助教授遺伝子実験施設
    From Apr. 2002
  •  
    東京農工大学准教授遺伝子実験施設
    From 01 Apr. 2007
  •  
    東京農工大学准教授学術研究支援総合センター
    From 01 Apr. 2008

Education

  • Kobe University
    Faculty of Science
    生物学科
    To 1986, graduated
  • Kobe University
    Graduate School, Division of Natural Science
    生物学専攻
    To 1988, completed
  • Kobe University
    Graduate School, Division of National Science and Technology (except Gakushuin University, Konan University)
    資源生物科学専攻
    To 1991, completed

Degree

  • 学術博士

Current state of research and teaching activities

  • 生物は、自身がもつ多くの遺伝情報の中から、必要な時に、必要な場所で、必要な遺伝子を発現することにより、生命活動を営んでいます。このような遺伝情報発現のしくみがどのようになっているのかということは大変興味深いと思います。例えば、植物において、植物ホルモンのバランスを操作することにより、植物切片からカルスという細胞の塊を誘導したり、そこから、完全な植物体を再生したりできることは、私たちに感動を与えます。私たちの研究室では、様々な興味深い生命現象のうち、植物ウイルスが宿主植物に感染し、増殖するという現象に着目しています。ウイルスが自分自身の増殖・拡散を有利にするために宿主の遺伝子産物を利用しているしくみや、逆に、植物側が(抵抗性遺伝子をもつ場合)防御反応としてウイルスを認識し自ら細胞死を起こすことにより、ウイルスを局所に封じ込めるしくみについて分子レベルで研究を行っています。また、得られた知見を利用して、ウイルス抵抗性植物を作出するというような応用面の研究にも取り組んでいます。  私は、遺伝子実験施設の専任教員ですが、応用生物科学科の協力教員、大学院修士・博士課程の担当教員でもありますので、希望する学生は、私たちの研究室において一緒に研究を行うことができます。

Research Areas

  • Environmental Science/Agriculture Science, Plant protection science
  • Life Science, Molecular biology
  • Life Science, Plant molecular biology and physiology

Research Interests

  • plant molecular biology, plant viruses, plant immunity, cell death

Subject of research

  • 未利用バイオマスの有効活用を目指した細胞死誘導遺伝子の利用に関する研究
    From 2008
  • 細胞死誘導遺伝子を利用したウイルス封じ込め戦略に関する研究
    From 2005
  • Studies on the plant cell death induced by of viral infections.
    From 2005
  • Studies on the mocromolecular transport in plants: utilizing the cell-to-cell movement of tobacco mosaic virus as a model system.
    From 1996

Proposed theme of joint or funded research

  • Studies on the plant cell death induced by viral infections.
    タバコモザイクウイルスに対する抵抗性遺伝子Nをもつタバコにタバコモザイクウイルスが感染した時に誘導される過敏感細胞死の情報伝達経路について分子レベルで研究する。ウイルス感染に伴って誘導される細胞死とウイルスの局所への封じ込めとの関係について分子レベルで解析する。細胞死誘導遺伝子を利用して広範囲のウイルスに有効な抵抗性植物を作出するためのバイオ技術を開発する。
    wish to undertake joint research with industry and other organizations including private sector.

Grants-in-Aid for Scientific Research

  • 基盤研究(C)一般
    植物の細胞死によるウイルス封じ込め戦略に関する研究
    From 2007, To 2008
  • 基盤研究(C)(2)一般
    トマトモザイクウィルスの移行蛋白質による宿主遺伝子発現調節に関する研究
    From 2003, To 2004
  • 基盤研究(C)一般
    トマトモザイクウイルスの移行蛋白質による宿主遺伝子発現調節に関する研究
    From 2003, To 2004
  • 若手研究(B)
    植物自身の遺伝子を利用した環境にやさしいウイルス抵抗性植物の作出
    From 2001, To 2002
  • 若手研究(B)
    トマトモザイクウイルスの移行蛋白質と結合する宿主側蛋白質遺伝子の単離と機能解析
    From 1999, To 2000

Papers

  • Overexpression of NtERF5, belonging to the ethylene response factor gene family, inhibits potato virus X infection and enhances expression of jasmonic acid/ethylene signaling marker genes in tobacco
    Hiyori Suzuki · Tomoki Ito· Takuya Ogata · Yuki Tsukahara · Richard S. Nelson4 · Nobumitsu Sasaki · Yasuhiko Matsushita
    Journal of General Plant Pathology
    Springer
    24 Jan. 2024, Research paper (scientific journal), joint, DOI(公開)(r-map)
  • Overexpression of BBF3 encoding a tobacco Dof transcription factor alters gene expression and cell death induction during N‑mediated hypersensitivity to tobacco mosaic virus
    Taiga Suzuki · Mayu Fujita· Mio Suzuki· Kaori Shigeta· Richard S. Nelson· Yasuhiko Matsushita· Nobumitsu Sasaki
    Journal of General Plant Pathology
    Springer
    08 Nov. 2023, Research paper (scientific journal), joint, 90, DOI(公開)(r-map), 2, 13
  • Cell death signaling is competitively but coordinately regulated by repressor-type and activator-type ethylene response factors in tobacco (Nicotiana tabacum) plants
    Ogata T, Tsukahara Y, Ito T, Iimura M, Yamazaki K, Sasaki N, Matsushita Y
    Plant Biology
    WILEY
    Ethylene response factors (ERFs) comprise one of the largest transcription factor families in many plant species. Tobacco (Nicotiana tabacum) ERF3 (NtERF3) and other ERF-associated amphiphilic repression (EAR) motif-containing ERFs are known to function as transcriptional repressors. NtERF3 and several repressor-type ERFs induce cell death in tobacco leaves and are also associated with a defence response against tobacco mosaic virus (TMV). We investigated whether transcriptional activator-type NtERFs function together with NtERF3 in the defence response against TMV infection by performing transient ectopic expression, together with gene expression, chromatin immunoprecipitation (ChIP) and promoter analyses. Transient overexpression of NtERF2 and NtERF4 induced cell death in tobacco leaves, albeit later than that induced by NtERF3. Fusion of the EAR motif to the C-terminal end of NtERF2 and NtERF4 abolished their cell death-inducing ability. The expression of NtERF2 and NtERF4 was upregulated at the early phase of N gene-triggered hypersensitive response (HR) against TMV infection. The cell death phenotype induced by overexpression of wild-type NtERF2 and NtERF4 was suppressed by co-expression of an EAR motif-deficient form of NtERF3. Furthermore, ChIP and promoter analyses suggested that NtERF2, NtERF3 and NtERF4 positively or negatively regulate the expression of NtERF3 by binding to its promoter region. Overall, our results revealed the cell death-inducing abilities of genes encoding activator-type NtERFs, including NtERF2 and NtERF4, suggesting that the HR-cell death signalling via the repressor-type NtERF3 is competitively but coordinately regulated by these NtERFs.
    Aug. 2022, Research paper (scientific journal), joint, 24, 5, 1435-8603, DOI(公開)(r-map), 897, 909
  • Cooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses
    Ikeda, Chihiro; Taku, Kazuo; Miyazaki, Tsumugi; Shirai, Rikako; Nelson, Richard S.; Nyunoya, Hiroshi; Matsushita, Yasuhiko; Sasaki, Nobumitsu
    SCIENTIFIC REPORTS
    NATURE PORTFOLIO
    The tobacco virus resistance gene N contains four introns. Transient expression of transcripts from an N transgene containing these introns and driven by the native promoter in the presence of the elicitor of tobacco mosaic virus resulted in its increased expression. The requirement of the native promoter, the elicitor, or the individual introns for enhanced expression of N has not been fully studied. Here, we determined that 35S promoter-driven N transcript expression could be enhanced in the presence of the four introns regardless of the co-expression of the virus elicitor in tobacco. Function analyses using a series of N transgenes with different combination of introns revealed that the presence of intron 1 more so than intron 2 allowed higher accumulation of premature and mature N transcripts; however, both introns were important for not only enhanced gene expression but also for induction of cell death in tobacco and induced local resistance to spread of virus in Nicotiana benthamiana. Our findings indicate that introns 1 and 2 cooperatively contribute to N expression and virus resistance.
    29 Jul. 2021, Research paper (scientific journal), joint, 11, 1, 2045-2322, DOI(公開)(r-map), 15424
  • Fungal Carbonyl Sulfide Hydrolase of Trichoderma harzianum Strain THIF08 and Its Relationship with Clade D beta-Carbonic Anhydrases
    Masaki, Yoshihito; Iizuka, Ryuka; Kato, Hiromi; Kojima, Yuka; Ogawa, Takahiro; Yoshida, Makoto; Matsushita, Yasuhiko; Katayama, Yoko
    MICROBES AND ENVIRONMENTS
    JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
    Carbonyl sulfide (COS) is the most abundant and long-lived sulfur-containing gas in the atmosphere. Soil is the main sink of COS in the atmosphere and uptake is dominated by soil microorganisms; however, biochemical research has not yet been conducted on fungal COS degradation. COS hydrolase (COSase) was purified from Trichoderma harzianum strain THIF08, which degrades COS at concentrations higher than 10,000 parts per million by volume from atmospheric concentrations, and its gene cos (492 bp) was cloned. The recombinant protein purified from Escherichia coli expressing the cos gene converted COS to H2S. The deduced amino acid sequence of COSase (163 amino acids) was assigned to clade D in the phylogenetic tree of the beta-carbonic anhydrase (beta-CA) family, to which prokaryotic COSase and its structurally related enzymes belong. However, the COSase of strain THIF08 differed from the previously known prokaryotic COSase and its related enzymes due to its low reactivity to CO2 and inability to hydrolyze CS2. Sequence comparisons of the active site amino acids of clade D beta-CA family enzymes suggested that various Ascomycota. particularly Sordariomycetes and Eurotiomycetes. possess similar enzymes to the COSase of strain THIF08 with >80% identity. These fungal COSase were phylogenetically distant to prokaryotic clade D beta-CA family enzymes. These results suggest that various ascomycetes containing COSase contribute to the uptake of COS by soil.
    22 May 2021, Research paper (scientific journal), joint, 36, 2, 1342-6311, DOI(公開)(r-map)
  • Cell-death-independent antiviral response mediated by N resistance factor in Nicotiana benthamiana involves inhibited localization of tobamovirus movement protein to plasmodesmata
    Sasaki, Nobumitsu; Murakami, Tomoya; Yoshimoto, Nanae; Komatsu, Ken; Matsushita, Yasuhiko; Nyunoya, Hiroshi
    JOURNAL OF GENERAL PLANT PATHOLOGY
    SPRINGER JAPAN KK
    The tobacco N gene encodes the N resistance factor that recognizes a tobacco mosaic virus elicitor called p50 to induce a cell-death-associated hypersensitive reaction, which confines virus infections to local areas. Coexpression of N and p50 cDNAs in Nicotiana benthamiana induces resistance against virus infection without visible cell death. In this study, we examined how this N-mediated resistance in the heterologous plant inhibits virus infection. After inoculation with GFP-encoding tomato mosaic virus (ToMV), plants coexpressing N and p50 had fewer and smaller infection sites as the levels of coexpressed proteins of N and p50 increased. This antiviral response was associated with production of reactive oxygen species but not ion leakage or expression of PR1a (encoding a salicylic acid signaling marker). In addition, it was likely that GFP-tagged movement protein (MP) of ToMV seemed to have failed to localize at plasmodesmata during virus infection in leaves coexpressing N and p50. These findings indicate that the cell death-independent, N-mediated antiviral response in N. benthamiana may involve interference with the intracellular transport function of MP.
    May 2021, Research paper (scientific journal), joint, 87, 3, 1345-2630, DOI(公開)(r-map), 170, 177
  • Plant Protein-Mediated Inhibition of Virus Cell-to-Cell Movement: Far-Western Screening and Biological Analysis of a Plant Protein Interacting with a Viral Movement Protein
    Sasaki N, Matsushita Y, Nyunoya H
    Methods in Molecular Biology
    Human Press
    22 Jun. 2019, Research paper (scientific journal), joint, 2028, 978-1-4939-9634-6, DOI(公開)(r-map), 123, 144
  • Involvement of NtERF3 in the cell death signalling pathway mediated by SIPK/WIPK and WRKY1 in tobacco plants
    Ogata T, Okada H, Kawaide H, Takahashi H, Seo S, Mitsuhara I, Matsushita Y
    Plant Biology
    Jun. 2015, Research paper (scientific journal), joint, 17, DOI(公開)(r-map), 962, 972
  • Cloning and expression of a gene encoding a novel thermostable thiocyanate-degrading enzyme from a mesophilic alphaproteobacteria strain THI201.
    Hussain A, Ogawa T, Saito M, Sekine T, Nameki M, Matsushita Y, Hayashi T, Katayama Y
    Microbiology
    Sep. 2013, Research paper (scientific journal), joint, 159, 11, DOI(公開)(r-map), 2294, 2302
  • Analysis of the Cell Death-Inducing Ability of the Ethylene Response Factors in Group VIII of the AP2/ERF Family
    Ogata T, Kida Y, Tochigi M, Matsushita Y
    Plant Science
    Apr. 2013, Research paper (scientific journal), joint, 209, DOI(公開)(r-map), 12, 23
  • Carbonyl sulfide hydrolase from Thiobacillus thioparus strain THI115 is one of the β-carbonic anhydrase family enzymes.
    Ogawa T, Noguchi K, Saito M, Nagahata Y, Kato H, Ohtaki A, Nakayama H, Dohmae N, Matsushita Y, Odaka M, Yohda M, Nyunoya H, Katayama Y
    J Am Chem Soc.
    Mar. 2013, Research paper (scientific journal), joint, 135, 10, DOI(公開)(r-map), 3818, 3825
  • Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris.
    Nobumitsu Sasaki, Tatsuro Odawara, Shoko Nagai, Kazuma Yoshimura, Yasuhiko Matsushita, Hiroshi Nyunoya
    Physiological and Molecular Plant Pathology
    Feb. 2012, Research paper (scientific journal), joint, 78, DOI(公開)(r-map), 38, 44
  • Overexpression of tobacco ethylene response factor NtERF3 gene and its homologues from tobacco and rice induces hypersensitive response-like cell death in tobacco.
    Takuya Ogata, Yuma Kida, Tomoyuki Arai, Yasuyuki Kishi, Yuki Manago, Masato Murai, Yasuhiko Matsushita
    Journal of General Plant Pathology
    Dec. 2011, Research paper (scientific journal), joint, 78, DOI(公開)(r-map), 8, 17
  • Extracellular histone induces plasma hyaluronan-binding protein (factor VII activating protease) activation in vivo.
    Yamamichi S, Fujiwara Y, Kikuchi T, Nishitani M, Matsushita Y, Hasumi K
    Biochem Biophys Res Commun.
    Jun. 2011, Research paper (scientific journal), joint, 409, 3, DOI(公開)(r-map), 483, 488
  • Interference with Tomato mosaic virus cell-to-cell movement by transient overexpression of Arabidopsis thaliana KELP homologs from different plant species
    Sasaki N, Odawara T, Deguchi M, Matsushita Y, Nyunoya H
    J. Gen. Plant Pathol.
    Feb. 2010, Research paper (scientific journal), joint, 76, DOI(公開)(r-map), 69, 73
  • Polyamine-promoted autoactivation of plasma hyaluronan-binding protein.
    Yamamichi S, Nishitani M, Nishimura N, Matsushita Y, Hasumi K
    J. Thromb. Haemost.
    Oct. 2009, Research paper (scientific journal), joint, 8, DOI(公開)(r-map), 559, 566
  • Overexpression of putative transcriptional coactivator KELP interferes with Tomato mosaic virus cell-to-cell movement.
    N. Sasaki, T. Ogata, M. Deguchi, S. Nagai, A. Tamai, T. Meshi, S. Kawakami, Y. Watanabe, Y. Matsushita, H. Nyunoya
    Molecular Plant Pathology
    Nov. 2008, Research paper (scientific journal), joint, 10, 2, DOI(公開)(r-map), 161, 173
  • The TIR–NBS but not LRR domains of two novel N-like proteins are functionally competent to induce the elicitor p50-dependent hypersensitive response
    Jun-Shan Gao, Nobumitsu Sasaki, Hiromi Kanegae, Ken-ichi Konagaya, Kaori Takizawa, Naomi Hayashi, Yosuke Okano, Masahiro Kasahara, Yasuhiko Matsushita, Hiroshi Nyunoya
    Physiological and Molecular Plant Pathology
    Dec. 2007, Research paper (scientific journal), joint, 71, DOI(公開)(r-map), 78, 87
  • Activation of prothrombin by two subtilisin-like serine proteases from Acremonium sp.
    Chunli Liu; Yasuhiko Matsushita, Kosuke Shimizu, Koichi Makimura, Keiji Hasumi
    Biochemical and Biophysical Research Communications
    May 2007, Research paper (scientific journal), joint, 358, DOI(公開)(r-map), 356, 362
  • Functional expression of thiocyanate hydrolase is promoted by its activator protein, P15K.
    Kataoka S, Arakawa T, Hori S, Katayama Y, Hara Y, Matsushita Y, Nakayama H, Yohda M, Nyunoya H, Dohmae N, Maeda M, Odaka M.
    FEBS Letters
    Aug. 2006, Research paper (scientific journal), joint, 580, DOI(公開)(r-map), 4667, 4672
  • Brassinosteroid Deficiency Due to Truncated Steroid 5a-Reductase Causes Dwarfism in the lk Mutant of Pea
    Nomura T1, Jager CE, Kitasaka Y, Takeuchi K, Fukami M, Yoneyama K, Matsushita Y, Nyunoya H, Takatsuto S, Fujioka S, Smith JJ, Kerckhoffs LH, Reid JB, Yokota T.
    Plant Physiology
    Aug. 2004, Research paper (scientific journal), joint, 135, 2220, 2229
  • Interaction of tomato mosaic virus movement protein with tobacco RIO kinase.
    K. Yoshioka, Y. Matsushita, M. Kasahara, K. Konagaya and H. Nyunoya
    Molecules and Cells
    Apr. 2004, Research paper (scientific journal), joint, 17, 223, 229
  • Members of 14-3-3 protein isoforms interacting with both Tobacco mosaic virus resistance gene product N and the viral elicitor.
    K. Konagaya, Y. Matsushita, M. Kasahara and H. Nyunoya
    Journal of General Plant Pathology
    2004, Research paper (scientific journal), joint, 70, 221, 231
  • Two proton pump interactors identified from a direct phosphorylation screening of a rice cDNA library by using a recombinant BRI1 receptor kinase.
    S. Hirabayashi, Y. Matsushita, M. Sato, R. Oh-i, M. Kasahara, H. Abe and H. Nyunoya
    Plant Biotechnology
    2004, Research paper (scientific journal), joint, 21, 35, 45
  • The catalytic subunit of protein kinase CK2 phosphorylates in vitro the movement protein of Tomato mosaic virus.
    Y. Matsushita, M. Ohshima, K. Yoshioka, M. Nishiguchi and H. Nyunoya.
    J. Gen. Virol.
    Feb. 2003, Research paper (scientific journal), joint, 84, DOI(公開)(r-map), 497, 505
  • Cloning of a tobacco cDNA coding for a putative transcriptional coactivator MBF1 that interacts with the Tomato mosaic virus movement protein.
    Y. Matsushita, O. Miyakawa, M. Deguchi, M. Nishiguchi and H. Nyunoya.
    J. Exp. Bot.
    Jun. 2002, Research paper (scientific journal), joint, 53, DOI(公開)(r-map), 1531, 1532
  • Phosphorylation of the movement protein of Cucumber mosaic virus in transgenic tobacco plants.
    Y. Matsushita, K. Yoshioka, T. Shigyo, H. Takahashi and H. Nyunoya
    Virus Genes
    Jun. 2002, Research paper (scientific journal), joint, 24, 231, 234
  • Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility factor SRK of Brassica campestris.
    Y. Matsushita, T. Suzuki, R. Kubota, M. Mori, H. Shimosato, M. Watanabe, T. Kayano, T. Nishio and H. Nyunoya
    J. Exp. Bot.
    Apr. 2002, Research paper (scientific journal), joint, 53, DOI(公開)(r-map), 765, 767
  • Genetic and immunochemical characterization of thiocyanate-degrading bacteria in lake water.
    M. Yamasaki, Y. Matsushita, M. Namura, H. Nyunoya and Y. Katayama
    Appl. Environ. Microbiol.
    Feb. 2002, Research paper (scientific journal), joint, 68, DOI(公開)(r-map), 942, 946
  • The tomato mosaic tobamovirus movement protein interacts with a putative transcriptional coactivator KELP.
    Y. Matsushita, M. Deguchi, M. Youda, M. Nishiguchi and H. Nyunoya
    Molecules and Cells
    Aug. 2001, Research paper (scientific journal), joint, 12, 57, 66
  • The molecular characterization and in situ expression pattern of pea SCARECROW gene
    N. Sassa, Y. Matsushita, T. Nakamura, and H. Nyunoya
    Plant Cell Physiol.
    Apr. 2001, Research paper (scientific journal), joint, 42, DOI(公開)(r-map), 385, 394
  • In vitro phosphorylation of the movement protein of tomato mosaic tobamovirus by a cellular kinase
    Y. Matsushita, K. Hanazawa, K. Yoshioka, T. Oguchi, S. Kawakami, Y. Watanabe, M. Nishiguchi and H. Nyunoya
    J. Gen. Virol.
    Aug. 2000, Research paper (scientific journal), joint, 81, 2095, 2102
  • Cloning of genes coding for the three subunits of thiocyanate hydrolase of Thiobacillus thioparus THI 115 and their evolutionary relationships to nitrile hydratase.
    Y. Katayama, Y. Matsushita, M. Kaneko, M. Kondo, T. Mizuno and H. Nyunoya
    J. Bacteriol.
    May 1998, Research paper (scientific journal), joint, 180, 2583, 2589
  • Cloning and analysis of a cDNA encoding farnesyl diphosphate synthase from Artemisia annua.
    Y. Matsushita, W.-K. Kang and B. V. Charlwood
    Gene
    Jun. 1996, Research paper (scientific journal), joint, 172, DOI(公開)(r-map), 207, 209
  • Transport of mitoribosomal proteins, YmL13 and MRP7, into isolated mitochondria of Saccharomyces cerevisiae.
    Y. Matsushita and K. Isono
    Biochem. Mol. Biol. Int.
    Aug. 1993, Research paper (scientific journal), joint, 30, 911, 919
  • Mitochondrial transport of mitoribosomal proteins, YmL8 and YmL20, in Saccharomyces cerevisiae.
    Y. Matsushita and K. Isono
    Eur. J. Biochem.
    Jun. 1993, Research paper (scientific journal), joint, 214, DOI(公開)(r-map), 577, 585
  • Cloning and analysis of the nuclear gene or YmL33, a protein of the large subunit of the mitochondrial ribosome in Saccharomyces cerevisiae.
    W.-K. Kang, Y. Matsushita, L. Grohmann, H.-R. Graack, M. Kitakawa and K. Isono
    J. Bacteriol.
    Jul. 1991, Research paper (scientific journal), joint, 173, 4013, 4020
  • Cloning and analysis of YMR26, the nuclear gene for a mitochondrial ribosomal protein in Saccharomyces cerevisiae.
    W.-K. Kang, Y. Matsushita and K. Isono
    Mol. Gen. Genet.
    Mar. 1991, Research paper (scientific journal), joint, 225, DOI(公開)(r-map), 474, 482
  • Cloning and analysis of the nuclear genes for two mitochondrial ribosomal proteins in yeast.
    Y. Matsushita, M. Kitakawa and K. Isono
    Mol. Gen. Genet.
    Oct. 1989, Research paper (scientific journal), joint, 219, DOI(公開)(r-map), 119, 124
  • Cloning and molecular characterization of the gene rimL which encodes an enzyme acetylating ribosomal protein L12 of Escherichia coli K12.
    S. Tanaka, Y. Matsushita, A. Yoshikawa and K. Isono
    Mol. Gen. Genet.
    Jun. 1989, Research paper (scientific journal), joint, 217, DOI(公開)(r-map), 289, 293

Books and Other Publications

  • 植物RNAウイルスの移行蛋白質と相互作用する宿主因子のcDNAクローニング
    松下保彦, 丹生谷博
    日本植物病理学会
    01 Aug. 2000, 1345-8086
  • Structure and expression of Pea Scr gene
    Sassa N, Nakamura T, Yamada M, Matsushita Y and Nyunoya H
    Japanese Society for Biological Sciences in Space
    01 Oct. 2000, 0914-9201

Presentations

  • CRISPR/Cas9ゲノム編集によるBBF遺伝子ノックアウトタバコの作製
    令和2年度日本植物病理学会関東部会
    28 Sep. 2020, Oral presentation(general)
  • 青枯病菌への抵抗性におけるタバコDOF型転写因子BBF3の役割
    令和元年度日本植物病理学会関東部会
    20 Sep. 2019, Oral presentation(general)
  • タバコのMyb domain protein 92はN遺伝子を介したウイルス抵抗性の負の制御に関与する
    令和元年度日本植物病理学会関東部会
    19 Sep. 2019, Oral presentation(general)
  • トマトモザイクウイルスの移行タンパク質とタバコのレモリン膜タンパク質の相互作用解析
    令和元年度日本植物病理学会関東部会
    19 Sep. 2019, Oral presentation(general)
  • タバコのDof型転写因子遺伝子BBF2の過剰発現は青枯病菌への抵抗性誘導を抑制する
    令和元年度日本植物病理学会関東部会
    19 Sep. 2019, Oral presentation(general)
  • ウイルス抵抗性遺伝子 N のイントロン 4 に由来する small RNA の推定標的遺伝子の解析
    平成31年度日本植物病理学会大会
    19 Mar. 2019, Oral presentation(general)
  • 過剰発現タバコを用いた Dof 型転写因子 BBF2 の病害抵抗性における機能の解析
    平成31年度日本植物病理学会大会
    19 Mar. 2019, Oral presentation(general)
  • Production of genes BBF2 and BBF3 knockout tabacco by genome editing technique
    MBSJ2018
    30 Nov. 2018, Poster presentation
  • Functional analysis of alternative polyadenylation signal of virus resistance gene N
    MBSJ2018
    29 Nov. 2018, Poster presentation
  • Analysis of viral growth inhibitory effect of ethylene responsive factor NtERF5
    MBSJ2018
    28 Nov. 2018, Poster presentation
  • Analysis of cell death induction by ethylene response factor NtERF#208
    MBSJ2018
    28 Nov. 2018, Poster presentation
  • Analysis of transcriptional regulatory mechanism of resistance gene N by intron
    MBSJ2018
    28 Nov. 2018, Poster presentation
  • ウイルス抵抗性遺伝子Nのイントロンに由来するsmall RNAの標的候補遺伝子の解析
    MBSJ2018
    28 Nov. 2018, Poster presentation
  • Involvement of a membrane-associated protein remorin of Nicotiana tabacum in propagation of ToMV
    MBSJ2017(ConBio2017)
    08 Dec. 2017, Other
  • Cell death induction by expression of human caspase-3 in plants
    MBSJ2017(ConBio2017)
    07 Dec. 2017, Poster presentation
  • NtERF3の封じ込め効果に関する研究
    平成29年度植物感染生理談話会
    28 Jul. 2017, Poster presentation
  • 病害抵抗性誘導におけるBBF3転写因子の誘導機構の解析
    平成29年度植物感染生理談話会
    28 Jul. 2017, Poster presentation
  • 転写物の蓄積とウイルス感染抑制における抵抗性遺伝子N のイントロンの役割
    平成29年度植物感染生理談話会
    28 Jul. 2017, Poster presentation
  • 植物細胞内でのウイルスの増殖におけるレモリン膜タンパク質(Remorin)の機能解析
    平成29年度植物感染生理談話会
    28 Jul. 2017, Poster presentation
  • BBF2転写因子の抵抗性誘導制御機構の解析
    平成29年度植物感染生理談話会
    28 Jul. 2017, Poster presentation
  • Site-specific containment of a recombinant virus expressing a gene for an ethylene responsive-transcriptional repressor NtERF3b
    MBSJ2016
    30 Nov. 2016, Poster presentation
  • Interaction analysis of co-repressor TOPLESS and ethylene response factors of Arabidopsis thaliana
    The 100th Anniversary Meeting of the Phytopathological Society of Japan
    31 Mar. 2015, Other
  • The role of EAR motifs for the cell death inducibility of the ethylene response factor AtERF#078
    MBSJ2014
    26 Nov. 2014, Poster presentation
  • グループVIIIに属するエチレン応答性因子の細胞死誘導能と過敏感反応との関係の研究
    平成24年度 植物感染生理談話会
    31 Aug. 2012, Poster presentation
  • Several EAR motif-containing ERFs in group VIII are involved in HR cell death.
    IS-MPMI 2012 XV International Congress
    01 Aug. 2012, Poster presentation
  • Tobacco NtERF3 and several repressor-type ERF genes are involved in the HR induced by N gene.
    平成24年度日本植物病理学会大会
    29 Mar. 2012, Other
  • Overexpression of NtERF3 and several EAR-containing ERFs induces HR-like cell death in tobacco leaves.
    The 2nd Korea-Japan Joint Symposium
    27 Mar. 2012, Poster presentation
  • Transient Overexpression of Repressor-Type of ERF Genes Induces HR-Like Cell Death in Tobacco.
    第53回度日本植物生理学会年会
    16 Mar. 2012, Poster presentation
  • Site-specific containment of viruses with cell death-inducing genes NtERF3 or NtSIPK.
    MBSJ2011
    16 Dec. 2011, Poster presentation
  • Gene expression analysis of the group VIII ERFs during the hypersensitive reaction in tobacco plants.
    MBSJ2011
    16 Dec. 2011, Poster presentation
  • Transient overexpression of the tobacco transcriptional repressor NtERF3 gene and its homologues induces hypersensitive response-like cell death in tobacco.
    IUMS2011 (XV International Congress of Virology)
    13 Sep. 2011, Oral presentation(general)
  • GENE CLONING OF ENZYME THIOCYANATE HYDROLASE OF STRAIN THI201
    IUMS2011 (XIII International Congress of Bacteriology and Applied Microbiology)
    06 Sep. 2011, Poster presentation
  • Transient Overexpression of NtERF3 Homologues Coding for the EAR Motif-Containing Proteins Induces HR-Like Cell Death in Tobacco.
    平成23年度日本植物病理学会大会
    28 Mar. 2011, Oral presentation(general)
  • Cell death Induction by the transient overexpression of tobacco ERF family genes.
    平成23年度日本植物病理学会大会
    27 Mar. 2011, Poster presentation
  • A Strategy for the Local Containment of Viruses by Site-Specific Cell Death Induction.
    平成23年度日本植物病理学会大会
    27 Mar. 2011, Poster presentation
  • Transient Introduction of the Silencing-Construct for an Autophagy-Related Gene NtBECLIN1 by Agrobacterium Induces Cell Death-Like Phenotype in Tobacco.
    平成23年度日本植物病理学会大会
    27 Mar. 2011, Poster presentation
  • Transient overexpression of tobacco NtERF3 and its homologous genes induces HR-like cell death in tobacco.
    第52回度日本植物生理学会年会
    20 Mar. 2011, Oral presentation(general)
  • Transient overexpression of group VIIIa genes of AP2/ERF family induces cell death in tobacco.
    BMB2010
    08 Dec. 2010, Poster presentation
  • Transient Overexpression of the Transcription Repressor NtERF3 and Its Homologues Induces Hypersensitive Cell Death in Tobacco
    平成22年度日本植物病理学会大会
    19 Apr. 2010, Poster presentation
  • Local containment of viruses with cell death inducing genes NtERF3 or NtSIPK.
    MBSJ2009
    09 Dec. 2009, Poster presentation
  • Cell death induction by transient overexpression of NtERF3 homologues
    MBSJ2009
    09 Dec. 2009, Poster presentation
  • Containment of recombinant viruses harboring cell death-inducing genes in plants with no corresponding-resistance genes.
    平成21年度日本植物病理学会大会
    27 Mar. 2009, Poster presentation
  • Induction of hypersensitive-like cell death and containment of recombinant viruses harboring tobacco NtERF3 in plants with no corresponding-resistance gene.
    BMB2008
    10 Dec. 2008, Poster presentation
  • Overexpression of putative transcriptional coactivator KELP interferes with virus movement.
    9th International Congress of Plant Pathology
    27 Aug. 2008, Poster presentation
  • Two novel N-like genes encoding functionally competent domains to induce hypersensitive response.
    9th International Congress of Plant Pathology
    27 Aug. 2008, Poster presentation
  • Overexpression of the tobacco NtERF3 gene induces hypersensitive response-like cell death in plants.
    XIV. International Congress of Virology
    13 Aug. 2008, Poster presentation
  • Gene Expression Analysis of the NtERF3 gene in Tobacco mosaic virus-Infected Tobacco and Induction of the Hypersensitive Cell Death by Transient Overexpression.
    平成20年度日本植物病理学会大会
    27 Apr. 2008, Other
  • Alteration of Subcellular Localization of the Tomato mosaic virus Movement Protein by Transient Overexpression of KELP.
    平成20年度日本植物病理学会大会
    27 Apr. 2008, Other
  • Transient overexpression of tobacco NtERF3 gene induces hypersensitive cell death in plants
    BMB2007
    12 Dec. 2007, Other
  • Construction of chimeric cDNAs with Tobacco mosaic virus resistance gene (N) and N-like genes, and their functional assays for hypersensitive response induction in Nicotiana tabacum
    BMB2007
    12 Dec. 2007, Other
  • ウイルス抵抗性遺伝子N類似遺伝子の過敏感応答誘導性に関する解析
    平成19年度植物感染生理談話会
    Aug. 2007, Poster presentation
  • Analysis of Functional Domains of Tobacco mosaic virus (TMV) Resistance Gene Product N and N-Like Proteins
    平成19年度日本植物病理学会大会
    Mar. 2007, Oral presentation(general)
  • Overexpression of AtKELP, an Interactor of the Movement Protein of Tomato mosaic virus, Holds Virus Movement.
    平成19年度日本植物病理学会大会
    Mar. 2007, Oral presentation(general)
  • Isolation of cDNA clones similar to the virus resistance gene N from Nicotiana tabacum.
    International Symposium for Plant Immunity: From MAMPs/PAMPs Recognition to Gene-for-Gene Resistance
    Mar. 2007, Poster presentation
  • TOMATO MOSAIC VIRUS MOVEMENT PROTEIN INTERACTS WITH TOBACCO RIO KINASE THAT IS PHOSPHORYLATED BY PROTEIN KINASE CK2
    8th International congress of plant molecular biology
    Aug. 2006, Poster presentation

External funds

  • 次世代シーケンサーを利用した新規アプリケーションの開発
    cooperative research, From 01 Apr. 2011, To 31 Mar. 2012
  • 次世代シーケンサーを利用した新規アプリケーションの開発
    cooperative research, From 20 Jul. 2010, To 31 Mar. 2011
  • 抵抗性遺伝子下流の転写関連因子を活用した新規ウイルス抵抗性野菜の開発
    commissioned research, From 2003, To 2004
  • 抗ウイルス蛋白質の設計と抵抗性植物の作出
    commissioned research, From 1999, To 2001
  • 組換え型抗ウイルスタンパク質の生産と抵抗性植物の作出
    commissioned research, From 1997, To 1998

Professional Memberships

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  • International Society for Molecular Plant-Microbe Interactions
  • American Society of Plant Biologists


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