研究者データベース
井上 真紀INOUE Makiイノウエ マキ
| ■所属部署名 | 農学研究院 生物制御科学部門 | |||
| ■職名 | 教授 | |||
Last Updated :2023/05/01
業績情報
氏名・連絡先
氏名
イノウエ マキ, 井上 真紀, INOUE Maki
主たる所属・職名
農学研究院 生物制御科学部門, 教授
その他の所属
経歴
- 株式会社環境指標生物
自 2001年03月01日, 至 2003年05月15日 - 東京大学大学院農学生命科学研究科
非常勤職員
自 2003年07月01日, 至 2004年10月31日 - 国立環境研究所
特別研究員
自 2008年04月01日, 至 2013年03月31日 - 東京農工大学女性未来育成機構・大学院農学研究院
講師
自 2013年04月01日, 至 2020年01月31日 - 東京農工大学大学院農学研究院
准教授
自 2020年02月01日
学歴
学位
教育・研究活動状況
- 1. 森林害虫マイマイガと核多角体病ウイルスとの共進化：森林害虫マイマイガは約10年周期で大発生し、病気の蔓延によって終息する。マイマイガに高い特異性をもつマイマイガ核多角体病ウイルスにおける適応戦略を解明する。大発生メカニズム：種内・種間相互作用や個体群構造や発生の時系列データから、個体群動態の不安定化に関与する要因と、密度上昇に伴った広域分散を可能にする生理的・生態的形質の変化を解明する。 2. 外来種の侵入に伴う寄生者群集構造変動：受粉用昆虫として導入されたセイヨウオオマルハナバチの導入に伴った寄生者群集構造変動と、送粉者ネットワークを介した植物病原体の伝播動態を解明する。 3．ハナバチ病原性微胞子虫の生活環における隠された媒介者：社会性ハナバチ類の重要病原体Nosema属の宿主交代を含む生活環と巣寄生性ガとの共進化関係を解明する。 4．共生微生物による宿主操作：茶の重要害虫であるチャハマキには、早期オス殺しを引き起こすSpiroplasmaやWolbachiaに加えて、後期オス殺しRNAウイルスが感染しており、オス特異的致死メカニズムを解明する。
研究分野
研究キーワード
担当授業科目
- 応用生物科学専門実験
2021年, 専門科目等 - 応用生物科学共通実験
2021年, 専門科目等 - 植物保護学
2021年, 専門科目等 - バイオロジカルコントロール
2020年 - 生物的制御科学II
2020年 - 基礎ゼミ
2020年 - バイオロジカルコントロール
2019年 - 生物的制御科学II
2019年 - 生態学
2019年 - バイオロジカルコントロール
2018年 - 生物的制御学特論
2018年 - 生態学
2018年 - 植物保護学
2018年 - 応用生物科学共通実験
2018年 - 応用生物科学専門実験
2018年 - 生物制御科学論文演習Ⅰ
2017年 - 生物制御科学論文演習Ⅲ
2017年 - 科学英語論文演習Ⅱ
2017年 - 生物制御科学特別実験Ⅰ
2017年 - 生物制御科学特別実験Ⅲ
2017年 - 卒業論文
2017年 - 生物制御科学特論Ⅱ
2017年 - バイオロジカルコントロール
2017年 - バイオロジカルコントロール
2017年 - 生物制御科学特別実験Ⅱ
2017年 - 生物制御科学特別実験Ⅳ
2017年 - 生物制御科学論文演習Ⅱ
2017年 - 生物制御科学論文演習Ⅳ
2017年 - 実践的英語プレゼンテーションⅢ
2017年 - 生態学
2017年 - 三菱UFJ財団寄付講座：農学部特別講義Ⅲ
2017年 - 植物保護学
2017年 - 生物制御科学特論Ⅱ
2016年 - バイオロジカルコントロール
2016年 - 生物的制御学特論
2016年 - 応用昆虫学特論
2016年 - 三菱UFJ財団寄付講座：農学部特別講義Ⅲ
2016年 - 応用生物科学専門実験
2016年 - 植物保護学
2016年 - 応用生物科学共通実験
2016年 - バイオロジカルコントロール
2015年 - 生態学
2015年 - 融合科目２（生命農学分野における最新バイオサイエンス概論）
2015年 - 東京外国語大学連携講義
2015年 - 三菱UFJ財団寄付講座：農学部特別講義Ⅲ
2015年 - 植物保護学
2015年 - 応用生物科学共通実験
2015年 - 応用生物科学専門実験
2015年 - 融合科目（生命農学分野における最新バイオサイエンス概論）
2014年 - 生態学
2014年 - 生物的制御学特論
2014年 - バイオロジカルコントロール
2014年 - 生態学
2013年
科学研究費助成事業
- 国際共同研究加速基金（国際共同研究強化(B)）
ハマキガのオスを殺す共生細菌ボルバキアの進化プロセスの解明
自 2021年, 至 2024年 - 基盤研究(B)
生理・生態情報および時空間解析に基づくマイマイガ大発生メカニズムの解明
自 2019年, 至 2022年 - 基盤研究(C)
外来種アルゼンチンアリのパーソナリティ特性とスーパーコロニーの進化機構の解明
自 2013年, 至 2015年
論文
- A simple method to disperse eggs from lepidopteran scalelike egg masses and to observe embryogenesis
2021年01月, 研究論文（学術雑誌）, DOI(公開)(r-map) - Coexistence of Two Male-Killers and Their Impact on the Development of Oriental Tea TortrixHomona magnanima
Takamatsu, Takumi; Arai, Hiroshi; Abe, Nobuhiko; Nakai, Madoka; Kunimi, Yasuhisa; Inoue, Maki N.
MICROBIAL ECOLOGY
SPRINGER
Male-killing, the death of male offspring induced by maternally transmitted microbes, is classified as early, or late, male-killing. The primary advantage afforded by early male-killing, which typically occurs during embryogenesis, is the reallocation of resources to females, that would have otherwise been consumed by males. Meanwhile, the key advantage of late male-killing, which typically occurs during late larval development, is the maximized potential for horizontal transmission. To date, no studies have reported on the associated developmental and physiological effects of host coinfection with early and late male-killers, which may have a significant impact on the population dynamics of the male-killers. Here we used a lepidopteran tea pestHomona magnanimaas a model, which is a unique system wherein an early male-killer (aSpiroplasmabacterium) and a late male-killer (an RNA virus) can coexist in nature. An artificially established matriline, coinfected with bothSpiroplasmaand RNA virus, exhibited embryonic death (early male-killing) as seen in the host line singly infected withSpiroplasma. Moreover, the coinfected line also exhibited developmental retardation and low pupal weight similar to the host line singly infected with the RNA virus. A series of field surveys revealed thatSpiroplasma-RNA virus coinfection occurs in nature at a low frequency. Hence, although the two male-killers are capable of coexisting within theH. magnanimapopulation independently, high associated fitness cost appears to limit the prevalence of male-killer coinfection in the field host population.
2021年01月, 研究論文（学術雑誌）, 共同, 81, 1, 0095-3628, DOI(公開)(r-map), 193, 202 - The effects of Nosema ceranae (Microspora: Nosematidae) isolated from wild Apis cerana japonica (Hymenoptera: Apidae) on Apis mellifera
Hosaka, Yuki; Kato, Yuto; Hayashi, Shinya; Nakai, Madoka; Barribeau, Seth M.; Inoue, Maki N.
APPLIED ENTOMOLOGY AND ZOOLOGY
SPRINGER JAPAN KK
The microsporidian Nosema ceranae commonly infects the Asian honey bee Apis cerana Fabricius (Hymenoptera: Apidae) and poses a high potential risk to co-occurring A. mellifera. Here, we demonstrated the effect of N. ceranae isolated from a wild colony of A. cerana japonica in Tokyo on A. mellifera workers. Sequencing analyses of the Tokyo N. ceranae isolate using the SSU rRNA gene showed a high probability match to the European N. ceranae isolates. Experimental exposure of A. mellifera workers with 10(2)-10(4) spores of N. ceranae resulted in 100% infection but did not significantly affect mortality, despite spore load doubling over the duration of infection. Nosema ceranae populations are geographically mixed, likely by the worldwide commercial trade of honeybees for commercial pollination. More than 100 years of A. mellifera beekeeping in Japan with repeated importations may promote N. ceranae transmission among A. mellifera and A. cerana japonica populations. Our results did not detect the lethal effects of the N. ceranae infection on A. mellifera workers. However, N. ceranae infections sometimes cause the sublethal effects, such as digestive disruption and nutritional stress, potentially resulting in reduced colony-level productivity. Therefore, we need to monitor the N. ceranae infections on A. mellifera.
2021年08月, 研究論文（学術雑誌）, 共同, 56, 3, 0003-6862, DOI(公開)(r-map), 311, 317 - Confirmation of Oryctes rhinoceros nudivirus infections in G-haplotype coconut rhinoceros beetles (Oryctes rhinoceros) from Palauan PCR-positive populations
Tanaka, Shunsuke; Harrison, Robert L.; Arai, Hiroshi; Katayama, Yukie; Mizutani, Tetsuya; Inoue, Maki N.; Miles, Joel; Marshall, Sean D. G.; Kitalong, Christopher; Nakai, Madoka
SCIENTIFIC REPORTS
NATURE PORTFOLIO
Coconut rhinoceros beetle (CRB), Oryctes rhinoceros, is a pest of palm trees in the Pacific. Recently, a remarkable degree of palm damage reported in Guam, Hawaii, Papua New Guinea and Solomon Islands has been associated with a particular haplotype (clade I), known as CRB-G. In the Palau Archipelago, both CRB-G and another haplotype (clade IV) belonging to the CRB-S cluster coexist in the field. In this study, more than 75% of pheromone trap-captured adults of both haplotypes were Oryctes rhinoceros nudivirus (OrNV)-positive by PCR. No significant difference in OrNV prevalence between the haplotypes was detected. In PCR-positive CRB-G tissue specimens from Palau, viral particles were observed by electron microscopy. Hemocoel injection of CRB larvae with crude virus homogenates from these tissues resulted in viral infection and mortality. OrNV isolated from Palauan-sourced CRB was designated as OrNV-Palau1. Both OrNV-Palau1 and OrNV-X2B, a CRB biological control isolate released in the Pacific, were propagated using the FRI-AnCu-35 cell line for production of inoculum. However, the OrNV-Palau1 isolate exhibited lower viral production levels and longer larval survival times compared to OrNV-X2B in O. rhinoceros larvae. Full genome sequences of the OrNV-Palau1 and -X2B isolates were determined and found to be closely related to each other. Altogether these results suggest CRB adults in Palau are infected with a less virulent virus, which may affect the nature and extent of OrNV-induced pathology in Palauan populations of CRB.
2021年09月20日, 研究論文（学術雑誌）, 共同, 11, 1, 2045-2322, DOI(公開)(r-map) - Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae
Fujita, Ryosuke; Inoue, Maki N.; Takamatsu, Takumi; Arai, Hiroshi; Nishino, Mayu; Abe, Nobuhiko; Itokawa, Kentaro; Nakai, Madoka; Urayama, Syun-ichi; Chiba, Yuto; Amoa-Bosompem, Michael; Kunimi, Yasuhisa
FRONTIERS IN MICROBIOLOGY
FRONTIERS MEDIA SA
Late male-killing, a male-specific death after hatching, is a unique phenotype found in Homona magnanima, oriental tea tortrix. The male-killing agent was suspected to be an RNA virus, but details were unknown. We herein successfully isolated and identified the putative male-killing virus as Osugoroshi viruses (OGVs). The three RNA-dependent RNA polymerase genes detected were phylogenetically related to Partitiviridae, a group of segmented double-stranded RNA viruses. Purified dsRNA from a late male-killing strain of H. magnanima revealed 24 segments, in addition to the RdRps, with consensus terminal sequences. These segments included the previously found male-killing agents MK1068 (herein OGV-related RNA16) and MK1241 (OGV-related RNA7) RNAs. Ultramicroscopic observation of purified virions, which induced late male-killing in the progeny of injected moths, showed sizes typical of Partitiviridae. Mathematical modeling showed the importance of late male-killing in facilitating horizontal transmission of OGVs in an H. magnanima population. This study is the first report on the isolation of partiti-like virus from insects, and one thought to be associated with late male-killing, although the viral genomic contents and combinations in each virus are still unknown.
2021年01月20日, 研究論文（学術雑誌）, 共同, 11, DOI(公開)(r-map) - Susceptibility of the Gypsy Moth Lymantria dispar (Lepidoptera: Erebidae) to Nosema pyrausta (Microsporidia: Nosematidae)
Kononchuk, Anastasia G.; Martemyanov, Vyacheslav V.; Ignatieva, Anastasia N.; Belousova, Irina A.; Inoue, Maki N.; Tokarev, Yuri S.
INSECTS
MDPI
Simple Summary Microsporidia are widespread insect pathogens and a single species may infect different hosts. Nosema pyrausta from the corn borer was tested against the gypsy moth. Thirty days after larvae were fed with spores, infection was established in the salivary glands and fat body of pupae and prepupae. Up to 10% of insects became infected. The gypsy moth can be referred to as a resistant host of N. pyrausta. The gypsy moth, Lymantria dispar, is a notorious forest defoliator, and various pathogens are known to act as natural regulators of its population density. As a widespread herbivore with a broad range of inhabited areas and host plants, it is potentially exposed to parasitic microorganisms from other insect hosts. In the present paper, we determined the susceptibility of gypsy moth larvae to the microsporidium Nosema pyrausta from the European corn borer, Ostrinia nubilalis. Gypsy moth samples from two localities of Western Siberia were used. N. pyrausta developed infections in the salivary gland and adipose tissue of gypsy moth prepupae and pupae, forming spore masses after 30 days of alimentary exposure to the second instar larvae. Among the experimental groups, the infection levels ranged from 0 to 9.5%. Effects of a covert baculovirus infection, phenylthiourea pretreatment and feeding insects on an artificial diet versus natural foliage were not significant in terms of microsporidia prevalence levels. Thus, L. dispar showed a low level of susceptibility to a non-specific microsporidium. It can be referred to as a resistant model host and not an appropriate substitute host for laboratory propagation of the microsporidium.
2021年05月, 研究論文（学術雑誌）, 共同, 12, 5, DOI(公開)(r-map) - 日本産マイマイガの卵塊 から得られたカツオブシムシ科昆虫
澤畠拓夫, 瀬口翔太, 藤本 将徳, 松井 啓祐, 井上真紀
近畿大学農学部紀要
2021年, 研究論文（大学，研究機関紀要）, 共同, 54, 24, 27 - マイマイガの低密度期における木造建築物への産卵個体数の照明の有無による違い
澤畠拓夫, 井上真紀
近畿大学農学部紀要
2021年, 研究論文（大学，研究機関紀要）, 共同, 54, 18, 23 - Coexistence of two male-killers and their impacts on the development of oriental tea tortrix Homona magnanima
Takamatsu T, Arai T, Abe N, Nakai M, Kunimi Y, Inoue MN
Microbial Ecology
2020年, 研究論文（学術雑誌）, 共同, 81, DOI(公開)(r-map), 193, 202 - Closely Related Male-Killing and Nonmale-Killing Wolbachia Strains in the Oriental Tea Tortrix Homona magnanima
Arai, Hiroshi; Lin, Shiou Ruei; Nakai, Madoka; Kunimi, Yasuhisa; Inoue, Maki N.
MICROBIAL ECOLOGY
SPRINGER
Wolbachia are inherited intracellular bacteria that cause male-specific death in some arthropods, called male-killing. To date, three Wolbachia strains have been identified in the oriental tea tortrix Homona magnanima (Tortricidae, Lepidoptera); however, none of these caused male-killing in the Japanese population. Here, we describe a male-killing Wolbachia strain in Taiwanese H. magnanima. From field-collected H. magnanima, two female-biased host lines were established, and antibiotic treatments revealed Wolbachia (wHm-t) as the causative agent of male-killing. The wsp and MLST genes in wHm-t are identical to corresponding genes in the nonmale-killing strain wHm-c from the Japanese population, implying a close relationship of the two strains. Crossing the Japanese and Taiwanese H. magnanima revealed that Wolbachia genotype rather than the host genetic background was responsible for the presence of the male-killing phenotype. Quantitative PCR analyses revealed that the density of wHm-t was higher than that of other Wolbachia strains in H. magnanima, including wHm-c. The densities of wHm-t were also heterogeneous between host lines. Notably, wHm-t in the low-density and high-density lines carried identical wsp and MLST genes but had distinct lethal patterns. Furthermore, over 90% of field-collected lines of H. magnanima in Taiwan were infected with wHm-t, although not all host lines harboring wHm-t showed male-killing. The host lines that showed male-killing harbored a high density of Wolbachia compared to the host lines that did not show male-killing. Thus, the differences in the phenotypes appear to be dependent on biological and genetic characteristics of closely related Wolbachia strains.
2020年05月, 研究論文（学術雑誌）, 共同, 79, 4, 0095-3628, DOI(公開)(r-map), 1011, 1020 - Reassessment of the status of Lymantria albescens and Lymantria postalba (Lepidoptera: Erebidae: Lymantriinae) as distinct 'Asian gypsy moth' species, using both mitochondrial and nuclear sequence data
Djoumad, Abdelmadjid; Nisole, Audrey; Stewart, Don; Holden, Dave; Zahiri, Reza; Inoue, Maki N.; Martemyanov, Viatcheslav V.; Levesque, Roger C.; Hamelin, Richard C.; Cusson, Michel
SYSTEMATIC ENTOMOLOGY
WILEY
For regulatory purposes, the name 'Asian gypsy moth' refers to a group of closely related Asian Lymantria species and subspecies whose female moths display flight capability, a trait believed to confer enhanced invasiveness relative to the European gypsy moth, Lymantria dispar dispar, whose females are flightless. Lymantria albescens and Lymantria postalba are Asian gypsy moths occurring in the southern Ryukyu Islands and in the northern Ryukyu and adjacent Kyushu and Shikoku Islands of Japan, respectively. Although once considered subspecies of L. dispar, their status as distinct species, relative to the latter, is now well established. While postalba was subsequently considered a subspecies of L. albescens, largely on the basis of differences in forewing ground colour in males, both taxa were later given distinct species status by Pogue & Schaefer (2007) following their revision of the genus Lymantria. Here, we re-examined the validity of this revised status through the sequencing of a large portion of the mitochondrial genome (c. 60%) and multiple nuclear marker genes [elongation factor 1-alpha (Ef-1 alpha), wingless (Wgl), internal transcribed spacer 2 (ITS-2), ribosomal protein S5 (RpS5)] in representative specimens of both taxa and other Lymantria species, including L. monacha, L. xylina, L. mathura and members of the L. dispar + L. umbrosa clade. A comparison of the number of substitutions in these genomic regions among the taxa we considered showed lower or equivalent variation between L. albescens and L. postalba compared with subspecies of L. dispar, for mitochondrial and nuclear sequences, respectively. This finding was reflected in the maximum likelihood trees generated independently for mitochondrial and nuclear data, where L. albescens and L. postalba formed, in both analyses, a short-branch sister clade basal to the L. dispar + L. umbrosa clade. We further sequenced three markers [cytochrome c oxydase 1 (COI), EF-1 alpha, Wgl] in multiple L. albescens-L. postalba specimens collected along a south-to-north transect across the Ryukyu Arc and observed no clear distinction among the sampled specimens as a function of taxonomic designation. We conclude that L. albescens and L. postalba form a single species, with postalba representing a darker-winged morph along an apparent south-to-north wing colour cline. Accordingly, L. postalba is relegated to synonymy under L. albescens (syn.n.).
2020年04月, 研究論文（学術雑誌）, 共同, 45, 2, 0307-6970, DOI(公開)(r-map), 493, 504 - Direct and sensitive detection of a microsporidian parasite of bumblebees using loop-mediated isothermal amplification (LAMP)
Kato, Yuto; Yanagisawa, Takahiro; Nakai, Madoka; Komatsu, Ken; Inoue, Maki N.
SCIENTIFIC REPORTS
NATURE PUBLISHING GROUP
The reduction of bumblebee populations has been reported in the last decades, and the microsporidian parasite Nosema bombi is considered as one of the factors contributing to such reduction. Although the decline of bee populations affects both wild plants and human food supply, the effects of Nosema spp. infections are not known because it is difficult to obtain infective spores from wild bees due to their low prevalence. Microscopical observation of fecal samples or midgut homogenates and/or PCR are generally used for N. bombi detection. However, the germination rate of microsporidian spore declines if they are kept at 4 degrees C for a long time or frozen. It is therefore crucial to minimize the diagnosis and isolation time of infective spores from field-collected samples. Therefore, we performed a loop-mediated isothermal amplification (LAMP) assay for the direct detection of N. bombi in bumblebee midgut homogenates. Using this method, we could detect N. bombi from individuals from which it was visible under the microscope and directly from wild individuals.
2020年01月24日, 研究論文（学術雑誌）, 共同, 10, 1, 2045-2322, DOI(公開)(r-map), 1118 - マイマイガの産卵場所としての広葉樹
澤畠拓夫, 石倉明莉, 井上真紀
近畿大学農学部紀要
2019年, 研究論文（大学，研究機関紀要）, 共同, 52, 27, 32 - Effects of Fipronil on Non-target Ants and Other Invertebrates in a Program for Eradication of the Argentine Ant, Linepithema humile
Sakamoto, Y.; Hayashi, T., I; Inoue, M. N.; Ohnishi, H.; Kishimoto, T.; Goka, K.
SOCIOBIOLOGY
UNIV ESTADUAL FEIRA SANTANA
Pesticides are frequently used to eradicate invasive ant species, but pose ecological harm. Previous studies assessed non-target effects only in terms of the increase or decrease of abundance or species richness after pesticide applications. Positive effects of the release from pressure caused by invasive ant species have not been considered so far. To more accurately assess pesticide effects in the field, the nontarget effects of pesticides should be considered separately from the positive effects of such releases. Here, we used monitoring data of ants and other invertebrates collected in a program for the eradication of the Argentine ant, Linepithema humile (Mayr), using fipronil. First, we separately assessed the effects of L. humile abundance and fipronil exposure on non-target ants and other invertebrates using generalized linear models. The abundance of L. humile and the number of pesticide treatments were negatively associated with the total number of non-target individuals and taxonomic richness. We also noted negative relationships between the number of individuals of some ant species and other invertebrate taxonomic groups. The L. humile x pesticide interaction was significant, suggesting that the abundance of L. humile affected the level of impact of pesticide treatment on non-target fauna. Second, we evaluated the dynamics of non-target ant communities for 3 years using principal response curve analyses. Non-target ant communities treated with fipronil continuously for 3 years recovered little, whereas those treated for 1 year recovered to the level of the untreated and non-invaded environment.
2019年06月, 研究論文（学術雑誌）, 共同, 66, 2, 0361-6525, DOI(公開)(r-map), 227, 238 - Population dynamics and geographical distribution of the gypsy moth, Lymantria dispar, in Japan
Inoue, Maki N.; Suzuki-Ohno, Yukari; Haga, Yuri; Aarai, Hiroshi; Sano, Tomoya; Martemyanov, Vyacheslav V.; Kunimi, Yasuhisa
FOREST ECOLOGY AND MANAGEMENT
ELSEVIER SCIENCE BV
The gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), is distributed throughout most of the northern hemisphere and known as one of the most significant insect pest both in its native and introduced regions. The aim of this study was to examine whether there are differences in the outbreak pattern of L dispar, and these differences can be explained by different subspecies or divided populations across Japan for appropriate management of L. dispar. We reviewed the records of outbreaks of L. dispar in Japan using available literature and internet sources. We also examined phylogenetic relationships between L. dispar populations in Japan and other global populations to clarify current debated classification of the Japanese L. dispar. We then estimated the distribution of Japanese L. dispar with a species distribution model (Maxent). Phylogenetic mtDNA analysis revealed that Lymantria species consisted of four clusters: the first cluster contained three L. dispar subspecies from Europe and continental Asia (classified into L. dispar dispar), the populations of Honshu and western Hokkaido (L. dispar asiatica), and the central and eastern Hokkaido populations of L. dispar hokkaidoensis, while the second and third clusters comprised L. albescens and L. postalba, respectively. According to historical records, L. dispar outbreaks occur in approximately 11 cast(year as numeric)-cycles in Hokkaido, regardless of its classification at the subspecies level. Within northeastern Japan, L. dispar outbreaks occurred simultaneously from 2014 to 2015, whereas there were few reports of outbreaks in southwestern Japan. The distribution ranges of L. dispar were determined mainly by the maximum depth of snow cover (100-250 cm), forest patch area (0-0.6 km(2)), and elevation (600-1400 m); clearly divided at the species level but not at the subspecies or divided population level. On the other hand, the forest types are likely to contribute to the population dynamics and geographical distribution of L. dispar.
2019年02月28日, 研究論文（学術雑誌）, 共同, 434, 0378-1127, DOI(公開)(r-map), 154, 164 - Multiple Infection and Reproductive Manipulations of Wolbachia in Homona magnanima (Lepidoptera: Tortricidae)
Arai, Hiroshi; Hirano, Tatsuya; Akizuki, Naoya; Abe, Akane; Nakai, Madoka; Kunimi, Yasuhisa; Inoue, Maki N.
MICROBIAL ECOLOGY
SPRINGER
Endosymbiotic bacterium Wolbachia interacts with host in either a mutualistic or parasitic manner. Wolbachia is frequently identified in various arthropod species, and to date, Wolbachia infections have been detected in different insects. Here, we found a triple Wolbachia infection in Homona magnanima, a serious tea pest, and investigated the effects of three infecting Wolbachia strains (wHm-a, -b, and -c) on the host. Starting with the triple-infected host line (W-abc), which was collected in western Tokyo in 1999 and maintained in laboratory, we established an uninfected line (W-) and three singly infected lines (W-a, W-b, and W-c) using antibiotics. Mating experiments with the host lines revealed that only wHm-b induced cytoplasmic incompatibility (CI) in H. magnanima, with the intensities of CI different between the W-b and W-abc lines. Regarding mutualistic effects, wHm-c shortened larval development time and increased pupal weight in both the W-c and W-abc lines to the same extent, whereas no distinct phenotype was observed in lines singly infected with wHm-a. Based on quantitative PCR analysis, Wolbachia density in the W-a line was higher than in the other host lines (p<0.01, n=10). Wolbachia density in the W-b line was also higher than in the W-c and W-abc lines, while no difference was observed between the W-c and W-abc lines. These results indicate that the difference in the CI intensity between a single or multiple infection may be attributed to the difference in wHm-b density. However, no correlation was observed between mutualistic effects and Wolbachia density.
2019年01月, 研究論文（学術雑誌）, 共同, 77, 1, 0095-3628, DOI(公開)(r-map), 257, 266 - マイマイガの卵塊の色と産卵環境の地域性
澤畠拓夫, 井上真紀
虫と自然
2018年, （MISC）総説・解説（商業誌）, 共同, 51, 20, 23 - An ascovirus isolated from Spodoptera litura (Noctuidae: Lepidoptera) transmitted by the generalist endoparasitoid Meteorus pulchricornis (Braconidae: Hymenoptera)
Arai, Eiko; Ishii, Kazuo; Ishii, Hiroki; Sagawa, Shiori; Makiyama, Nao; Mizutani, Tetsuya; Omatsu, Tsutomu; Katayama, Yukie; Kunimi, Yasuhisa; Inoue, Maki N.; Nakai, Madoka
JOURNAL OF GENERAL VIROLOGY
MICROBIOLOGY SOC
The family Ascoviridae is a recently described virus family whose members are transmitted by parasitoids and cause chronic and lethal infections in lepidopteran insects. Little is known about the biology and ecology of ascoviruses, and few isolates have been found outside the United States. We report here the isolation of a new ascovirus variant from Spodoptera litura in Japan. Full genome sequence and phylogenetic analyses showed that this virus was closely related to variants in Heliothis virescens ascovirus-3a, and it was named HvAV-3j. HvAV-3j has a DNA genome of 191 718 bp, with 189 putative ORFs and a GC content of 45.6 %, and is highly similar to HvAV-3h, which was isolated in China. In a field survey, the endoparasitoid Meteorus pulchricornis caused a high percentage of parasitization in populations of S. litura larvae, and under laboratory conditions M. pulchricornis was able to transmit HvAV-3j from infected to uninfected larvae by oviposition. Meteorus pulchricornis is thus likely to be a major vector for HvAV-3j transmission in Japan. This species is recognized here for the first time as a vector of ascoviruses that parasitizes a range of host species that extends across families.
2018年, 研究論文（学術雑誌）, 共同, 99, 4, 0022-1317, DOI(公開)(r-map), 574, 584 - Differential expression of a fructose receptor gene in honey bee workers according to age and behavioral role
Takada, Tomoyuki; Sasaki, Taiyo; Sato, Ryoichi; Kikuta, Shingo; Inoue, Maki N.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
WILEY
Honey bee (Apis mellifera) workers contribute to the maintenance of colonies in various ways. The primary functions of workers are divided into two types depending on age: young workers (nurses) primarily engage in such behaviors as cleaning and food handling within the hive, whereas older workers (foragers) acquire floral nutrients beyond the colony. Concomitant with this age-dependent change in activity, physiological changes occur in the tissues and organs of workers. Nurses supply younger larvae with honey containing high levels of glucose and supply older larvae with honey containing high levels of fructose. Given that nurses must determine both the concentration and type of sugar used in honey, gustatory receptors (Gr) expressed in the chemosensory organs likely play a role in distinguishing between sugars. Glucose is recognized by Gr1 in honey bees (AmGr1); however, it remains unclear which Gr are responsible for fructose recognition. This study aimed to identify fructose receptors in honey bees and reported that AmGr3, when transiently expressed in Xenopus oocytes, responded only to fructose, and to no other sugars. We analyzed expression levels of AmGr3 to identify which tissues and organs of workers are involved in fructose recognition and determined that expression of AmGr3 was particularly high in the antennae and legs of nurses. Our results suggest that nurses use their antennae and legs to recognize fructose, and that AmGr3 functions as an accurate nutrient sensor used to maintain food quality in honey bee hives.
2018年, 研究論文（学術雑誌）, 共同, 97, 2, 0739-4462, DOI(公開)(r-map) - Morphological properties of the occlusion body of Adoxophyes orana granulovirus
Tsuruta, Keiko; Wennmann, Jorg T.; Kunimi, Yasuhisa; Inoue, Maki N.; Nakai, Madoka
JOURNAL OF INVERTEBRATE PATHOLOGY
ACADEMIC PRESS INC ELSEVIER SCIENCE
A granulovirus (GV) that produces occlusion bodies (OBs) having an unusual morphology was found in an Adoxophyes sp. (Lepidoptera: Tortricidae) larva in a tea field in Miyazaki Prefecture, Japan. This isolate is considered to be a mutant of Adoxophyes orana granulovirus, designated AdorGV-M, because the nucleotide sequence of its genome is 99.7% identical to that of an English isolate of AdorGV, AdorGV-E. AdorGV-E produces typical ovocylindrical OBs that contain one occlusion-derived virus (ODV) per OB. On the other hand, AdorGV-M produces large cuboidal OBs, but the number of ODVs per OB was unknown. In this study, we quantified viral DNA in OBs of both AdorGV-E and -M, and determined the number of ODVs occluded in an OB of AdorGV-M. The two isolates had the same quantity of viral DNA in each OB, and we thus confirmed that one OB of AdorGV-M contains one ODV. To investigate the process of OB formation, fat body tissue of A. honmai larvae inoculated with each isolate was observed in a time course by transmission electron microscopy, and OB sizes were measured from micrographs. The main difference in OB formation was that AdorGV-M required more time to mature than AdorGV-E. In AdorGV-E, ODVs began to be covered from one end with an ovocylindrical OB at 96 h post inoculation (hpi), and most of them were completely occluded at 120 hpi. Occlusion of AdorGV-M ODVs also began at 96 hpi, but the OB shape was cuboidal. Moreover, the OB size of AdorGV-M was similar to that of AdorGV-E at 120 hpi, but continued to grow until 192 hpi. AdorGV-M thus took more time to complete OB formation. Consequently, AdorGV-E has mature OBs with a diameter 0.22 mu m and length 0.39 mu m, but those of AdorGV-M are 1.34 x 1.23 mu m.
2018年, 研究論文（学術雑誌）, 共同, 154, 0022-2011, DOI(公開)(r-map), 58, 64 - 近畿大学奈良キャンパスにおけるマイマイガの大発生5年後の産卵状況
澤畠拓夫・井上真紀
近畿大学農学部紀要
2018年, 研究論文（大学，研究機関紀要）, 共同, 51, 76, 78 - Identification of an Early Male-Killing Agent in the Oriental Tea Tortrix, Homona magnanima
Tsugeno Y, Koyama H, Takamatsu T, Nakai M, Kunimi Y, Inoue MN
Journal of Heredity
OXFORD UNIV PRESS INC
Arthropods are frequently infected with inherited symbionts, which sometimes confer fitness benefits on female hosts or manipulate host reproduction. Early male killing, in which infected males die during embryogenesis, is induced by some bacteria, such as Wolbachia and Spiroplasma. A female-biased sex ratio has been found in Homona magnanima, collected from a tea plantation in Japan. Here, we examined the male-killing trait in H. magnanima and identified the agent that induces early male killing. The sex ratio distortion (SR) strain produced only females and no males, and its egg hatch rate was significantly lower than that of the normal (N) strain. The N strain was infected with only Wolbachia, whereas the SR strain was infected with both Wolbachia and Spiroplasma. Antibiotic treatment with 0.10% tetracycline restored the 1:1 sex ratio in the SR strain. Females treated with 0.05% tetracycline were positive for Spiroplasma but not for Wolbachia and showed a female-biased sex ratio, whereas Wolbachia-positive females did not revert to male killing. When inoculated with a homogenate of the SR strain female, females infected with only Spiroplasma produced female-biased offspring. Sequence analysis of the 16S rRNA gene revealed that Spiroplasma sp. of H. magnanima belonged to the ixodetis clade. These results indicate that Spiroplasma was responsible for male killing in H. magnanima. Late male killing is induced in H. magnanima by an RNA-like virus, and therefore this is the first case in which different male-killing agents expressed at different times in the life cycle have been found within one host species.
2017年, 研究論文（学術雑誌）, 共同, 108, 5, 0022-1503, DOI(公開)(r-map), 553, 560 - Midgut-based resistance to oral infection by a nucleopolyhedrovirus in the laboratory-selected strain of the smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae)
Iwata, Kento; Haas-Stapleton, Eric; Kunimi, Yasuhisa; Inoue, Maki N.; Nakai, Madoka
Journal of General Virology
MICROBIOLOGY SOC
A strain of Adoxophyes honmai resistant to Adoxophyes honmai nucleopolyhedrovirus (AdhoNPV) was established from a field-collected colony by repeated selection. Fifth-instar larvae of this resistant strain (R-strain) had over 66 666-fold greater resistance in terms of 50% lethal concentration values to oral infection of AdhoNPV than non-selected strain larvae (susceptible for AdhoNPV; S2-strain). In this study, the mechanism of resistance to AdhoNPV was determined in R- strain larvae. An assessment of viral genome replication in AdhoNPV-infected S2- and R-strain larvae by quantitative PCR showed no viral genome replication occurring in R-strain larvae. Transcription of AdhoNPV ie-1, vp39 and polyhedrin genes was also not detected in R-strain midgut cells. Besides, a fluorescent brightener had no effect on AdhoNPV infection in either S2- or R-strain. However, binding and fusion of occlusion-derived virus with R-strain were significantly lower than those of S2strain. These findings suggest that R-strain Adoxophyes honmai larvae possess a midgut-based resistance to oral infection by AdhoNPV in which midgut epithelial cells are infected less efficiently.
2017年, 研究論文（学術雑誌）, 共同, 98, 2, 0022-1317, DOI(公開)(r-map), 296, 304 - Detection of bacterial symbionts (Wolbachia, Spiroplasma) and eukaryotic pathogen (Microsporidia) in Japanese populations of gypsy moth species (Lymantria spp.)
Ilinsky YY, Tokarev YS, Bykov RA, Yudina MA, Pavlushin SV, Inoue MN, Martemyanov VV
Euroasian Entomological Journal
2017年, 研究論文（学術雑誌）, 共同, 16, 1, 1, 5 - Genetic structure and potential environmental determinants of local genetic diversity in Japanese honeybees (Apis cerana japonica)
Nagamitsu T, Yasuda M, Saito-Morooka F, Inoue MN, Nishiyama M, Goka K, Sugiura S, Maeto K, Okabe K, Taki H
PLOS ONE
2016年, 研究論文（学術雑誌）, 共同, DOI(公開)(r-map), 10.1371/journal.pone.0167233 - Effect of granulovirus infection of Spodoptera litura (Lepidoptera: Noctuidae) larvae on development of the endoparasitoid Chelonus inanitus (Hymenoptera: Braconidae)
Azam A, Kunimi Y, Inoue MN, Nakai M
Applied Entomology and Zoology
2016年, 研究論文（学術雑誌）, 共同, 50, 3, 479, 488 - 寄生蜂に対する毒素タンパク質を産生する昆虫ウイルス
仲井まどか・国見裕久・井上真紀
昆虫と自然
2016年, （MISC）総説・解説（商業誌）, 共同, 51, 3, 20, 23 - 昆虫の性を操る微生物：オス殺し細菌とウイルス
井上真紀・仲井まどか・国見裕久
昆虫と自然
2016年, （MISC）総説・解説（商業誌）, 共同, 51, 3, 24, 27 - Queen execution increases relatedness among workers of the invasive Argentine ant, Linepithema humile
Inoue MN, Ito F, Goka K
Ecology and Evolution
2015年, 研究論文（学術雑誌）, 共同, 5, 18, 4098, 4107 - Ecological impacts on native ant and ground-dwelling animal communities through the Argentine ant (Linepithema humile) management in Japan
Inoue MN, Saito-Morooka F, Suzuki K, Nomura T, Hayasaka D, Kishimoto T, Sugimaru K, Sugiyama T, Goka K
Applied Entomology and Zoology
2015年, 研究論文（学術雑誌）, 共同, 50, 3, 331, 339 - Juvenile hormone (JH) esterase activity but not JH epoxide hydrolase activity is downregulated in larval Adoxophyes honmai following nucleopolyhedroviruses infection
Saito Y, Kamita SG, Hammock BD, Kunimi Y, Inoue MN, Nakai M
Journal of Insect Physiology
2015年, 研究論文（学術雑誌）, 共同, 80, 71, 80 - Different acute toxicity of fipronil baits on invasive Linepithema humile supercolonies and some non-target ground arthropods
Hayasaka D, Kuwayama N, Takeo A, Ishida T, Mano H, Inoue MN, Nagai T, Sa´nchez-Bayo F, Goka K, Sawahata T
Ecotoxicology
2015年, 研究論文（学術雑誌）, 共同, 24, 6, 1221, 1228 - Estimating colonization and invasion risk maps for Linepithema humile, in Japan
Moriguchi S, Inoue MN, Kishimoto T, Kameyama T, Ito F, Goka K
Journal of Asia-Pacific Entomology
2015年, 研究論文（学術雑誌）, 共同, 18, 2, 343–350 - Recent range expansion of the Argentine ant in Japan
Inoue MN, Sunamura E, Suhr EL, Ito F, Tatsuki F, Goka K
Diversity and Distribution
2013年, 研究論文（学術雑誌）, 共同, 19, 1, 29, 37 - 外来アリの世界侵略史と社会構造
井上真紀
地球環境
2012年, 単独, 17, 2, 119, 126 - Potential increase in mating frequency of queens in feral colonies of Bombus terrestris introduced into Japan
Inoue MN, Saito F, Tsuchida K, Goka K
Naturwissenschaften
2012年, 研究論文（学術雑誌）, 共同, 99, 10, 853, 861 - Size-dependent selection against small queens of the invasive bumblebee Bombus terrestris (L.) in Japan
Inoue MN
Entomologia Experimentalis et Applicata
2011年, 研究論文（学術雑誌）, 単独, 138, 65, 70 - Competition for flower resources and nest sites between Bombus terrestris (L.) and the Japanese native bumblebees
Inoue MN, Yokoyama J
Applied Entomology and Zoology
2010年, 研究論文（学術雑誌）, 共同, 45, 29, 35 - Status of the invasion and range expansion of an introduced bumblebee, Bombus terrestris (L.) in Japan
Yokoyama J, Inoue MN
Applied Entomology and Zoology
2010年, 研究論文（学術雑誌）, 共同, 45, 21, 27 - Reproductive disturbance risks to indigenous Japanese bumblebees from introduced Bombus terrestris
Tsuchida K., Kondo NI., Inoue MN, Goka K
Applied Entomology and Zoology
2010年, 研究論文（学術雑誌）, 共同, 45, 49, 58 - Colony growth and reproductive ability of feral nests of the introduced bumblebee Bombus terrestris in northern Japan
Inoue MN, Yokoyama J & Tsuchida K
Insectes Sociaux
2010年, 研究論文（学術雑誌）, 単独, 57, 29, 38 - Is Bombus terrestris (Hymenoptera: Apidae) a stronger competitor against native Japanese species? A comparison of foraging efficiency
Inoue MN, Makino TT, Yokoyama J, Sakai S
Applied Entomology and Zoology
2010年, 研究論文（学術雑誌）, 共同, 45, 1, 71, 75 - Applying geographic profiling used in the field of criminology for predicting the nest locations of bumble bees
Suzuki-Ohno Y, Inoue MN, Ohno K
Journal of Theoretical Biology
2010年, 研究論文（学術雑誌）, 共同, 265, 211, 217 - 小さな侵略者 アルゼンチンアリ
岸本年郎・井上真紀
地理
2009年, 共同, 54, 4, 68, 71 - The invasion of alien ant across continents with special reference to Argentine Ants and Red Imported Fire Ants
Inoue MN, Goka K
Biodiversity
2009年, 共同, 10, 67, 71 - Conservation ecological study of invasion of Bombus terrestris (Hymenoptera: Apidae) into a preserved area of the Notsuke Peninsula of eastern Hokkaido, Japan
Inoue MN, Ishikawa S, Inoue T, Washitani I
Applied Entomology and Zoology
2009年, 研究論文（学術雑誌）, 共同, 44, 3, 337, 342 - Displacement of Japanese native bumblebees by the recently introduced Bombus terrestris (L.) (Hymenoptera: Apidae)
Inoue MN, Yokoyama J, Washitani I
Journal of Insect Conservation
2008年, 研究論文（学術雑誌）, 共同, 12, 135, 146 - 根室半島におけるノサップマルハナバチの訪花植物に関する予察的研究
横山潤・井上真紀・伊藤誠夫・鷲谷いづみ
根室市歴史と自然の資料館紀要
2007年, 研究論文（大学，研究機関紀要）, 共同, 21, 59, 63 - 野付半島におけるセイヨウオオマルハナバチの定着状況と在来マルハナバチ相
井上真紀・菊池玲奈・石川聖江・横山潤・鷲谷いづみ
保全生態学研究
2007年, 研究論文（大学，研究機関紀要）, 共同, 12, 172, 175 - Effect of Weigela hortensis (Caprifoliaceae) floral morphology on pollinator behavior
Inoue MN, Yokoyama J
Plant Species Biology
2007年, 研究論文（学術雑誌）, 共同, 22, 77, 86 - Morphological variation in relation to flower use in bumblebees
Inoue MN, Yokoyama
Entomological Science
2006年, 研究論文（学術雑誌）, 共同, 9, 147, 159
著書
- バイオロジカルコントロール
2022年04月 - 外来アリのはなし（橋本佳明偏）：第8章 アルゼンチンアリ
朝倉書店
2020年05月 - 生態系の暮らし方 アジア視点の環境リスク－マネジメント（池文人・金子信博・松田裕之・茂岡忠義編著）：第13章遺伝子で見た野生生物の人為流動－外来アリ類を例に
井上真紀
東海大学出版会
2012年03月
研究発表、招待講演等
- 社会性昆虫マルハナバチのリスク評価手法の確立
日本農薬学会第46 回大会
2021年03月, 口頭発表（招待・特別） - 農業生態系における昆虫と共生
第3回日本生物防除協議会シンポジウム
2019年, 口頭発表（招待・特別） - チャハマキにおける共生微生物による性操作
第13回 昆虫病理研究会シンポジウム
2018年, 口頭発表（招待・特別） - チャハマキで後期オス殺しを誘導するOsugoroshi virusに関する研究
第13回 昆虫病理研究会シンポジウム
2018年, 口頭発表（招待・特別） - Identification of Osugroshi virus, a late male-killing virus in Homona magnanima
The 2018 International Congress of Invertebrate Pathology
2018年, 口頭発表（一般） - Late male killing caused by novel RNA viruses Partitiviridae in a tea pest, Homona magnanima
Wolbachia Conference 2018
2018年, 口頭発表（一般） - The third Male Killer: Characterization of Male-killing Wolbachia in oriental tea tortrix Homona magnanima
Wolbachia Conference 2018
2018年, 口頭発表（一般） - 社会性昆虫マルハナバチのリスク評価手法の確立
日本農薬学会第46 回大会
2021年, シンポジウム・ワークショップ パネル（公募） - 日本産マイマイガLymantria disparの発生動態と地理的分布パターン
第62回日本応用動物昆虫学会
2018年, 口頭発表（一般） - Sex ratio distortion in tea pest Homona magnanima: a complicated association of host and endosymbiotic microbes
Society for invertebrate pathology
2017年, ポスター発表 - Multiple infections and reproductive manipulations of Wolbachia in Homona magnanima
9th International Wolbachia Conference
2016年, ポスター発表 - The prevalence of two male-killing agents and ecological characteristic in Homona magnanima
9th International Wolbachia Conference
2016年, ポスター発表 - 有用昆虫における微胞子虫の感染とその影響
第12回 昆虫病理研究会シンポジウム
2016年09月16日, 口頭発表（招待・特別） - Ecological impacts on native ant and ground-dwelling animal communities through the invasive Argentine ant management in Japan
XVIII. International Plant Protection Congress
2015年08月26日, 口頭発表（招待・特別） - 侵略的外来生物の脅威とその防除対策
第19回農薬相模セミナー
2015年01月08日, 口頭発表（招待・特別） - 気づけば苦てが仕事になる
学生のための研究キャリアビジョンセミナー～女性研究者編～
2014年12月03日, 口頭発表（招待・特別） - 生態学的女性研究者論
日本昆虫学会第74回大会広島大会
2014年09月, 口頭発表（招待・特別） - Decline of native bumblebees (Bombus) and Nosema (Microspordia: Nosematidae) infections associated with introduction of the European bumblebee in Northern Japan
International Congress on Invertebrate Pathology
2014年09月, ポスター発表 - Recent range expansion and population genetics of the Argentine ant
International Union for the Study of Social Insects International Congress
2014年07月, ポスター発表
外部研究資金等
委員歴
メディア報道
- かっこいい農業
東京農工大学の井上真紀准教授が、農業と昆虫に関する本を紹介する。
ソトコト
自 2021年12月06日, 至 2021年12月06日 - 東京農工大学大学院農学研究院准教授｜井上真紀さんが選ぶ、「農度」を高める本5冊
東京農工大学の井上真紀准教授が、農業と昆虫に関する本を紹介する。
BIGLOBE／ソトコト
自 2022年01月13日, 至 2022年01月13日