半　智史NAKABA Satoshiナカバ　サトシ

業績情報

氏名・連絡先

• 氏名

  ナカバ　サトシ, 半　智史, NAKABA Satoshi

• Fax番号

  042-334-5700

• eメールアドレス

  nakabacc.tuat.ac.jp

• 個人ホームページ

  http://web.tuat.ac.jp/~tokusei/

主たる所属・職名

• 農学研究院　環境資源物質科学部門, 教授

その他の所属

• 農学部　環境資源科学科
• グローバルイノベーション研究院
• 連合農学研究科

経歴

• -
  東京農工大学大学院農学研究院　准教授
  自 2016年12月
• -
  東京農工大学大学院農学研究院　助教
  自 2011年12月, 至 2016年11月
• -
  東京農工大学農学府•農学部 産学官連携研究院
  自 2011年04月, 至 2011年11月
• -
  東京農工大学農学府•農学部　特別研究員
  自 2009年07月, 至 2011年03月
• -
  東京農工大学農学府•農学部　科研費等研究支援研究員
  自 2009年04月, 至 2009年06月
• -
  日本学術振興会特別研究員（DC2）
  自 2007年04月, 至 2009年03月

学歴

• 東京農工大学
  連合農学研究科
  資源・環境学専攻
  至 2009年03月, 修了, 博士
• 東京農工大学
  農学教育部
  環境資源物質科学専攻
  至 2006年03月, 修了, 修士
• 北海道大学
  農学部
  森林科学科
  至 2004年03月, 卒業

学位

• 博士（農学）

教育・研究活動状況

• 木質バイオマスにおける組織構造特性および材料特性の発現機構解明に向けた細胞生物学的解析

研究分野

• A189　ライフサイエンス, A40020　木質科学

研究キーワード

• 木材解剖学、細胞生物学、樹木生理学

研究テーマ

• 木質バイオマス形成に関わる細胞分化制御機構の解明
  自 9999
• イメージング技術を用いた木質バイオマスの特性評価手法の開発
  自 9999
• 生育環境が木質バイオマスの形成に与える影響メカニズムの解明
  自 9999

担当授業科目

• 環境資源科学実習A
  2023年, 専門科目等
• 生物学実験
  2023年, 教養科目等
• 環境資源科学実験Ⅲ（生物学応用）
  2023年, 専門科目等
• 森林実習
  2023年, 専門科目等
• 環境資源科学実験Ⅲ（生物学応用）
  2022年, 専門科目等
• 環境資源科学実習A
  2022年, 専門科目等
• 生物学実験
  2022年, 教養科目等

科学研究費助成事業

• 基盤研究(B)
  樹木の細胞壁三次元構造の多様性発現機構のイメージング解析
  自 2023年, 至 2023年
• 基盤研究（B）
  イチョウの材形成及び木材組織構造の網羅的解析から迫る木本植物の進化と環境適応性
  自 2023年, 至 2023年
• 基盤研究（B）
  樹木の土壌病害の早期発見手法の開発
  自 2023年, 至 2023年
• 基盤研究（B）
  ときに何十年も生存する樹木木部柔細胞の生活史―幹放射方向を区分する新基準の提案
  自 2023年, 至 2023年
• 基盤研究（B）
  13CO2パルスラベリングによる樹幹内炭素配分過程の解明と肥大成長モデルの構築
  自 2023年, 至 2023年
• 基盤研究(B)
  樹木の心材形成において見出された独自の細胞死プロセスの解明
  自 2023年, 至 2025年
• 基盤研究(B)
  樹木の細胞壁三次元構造の多様性発現機構のイメージング解析
  自 2022年, 至 2022年
• 基盤研究(B)
  樹木の土壌病害の早期発見手法の開発
  自 2022年, 至 2022年
• 基盤研究(B)
  ときに何十年も生存する樹木木部柔細胞の生活史―幹放射方向を区分する新基準の提案
  自 2022年, 至 2022年
• 基盤研究(B)
  イチョウの材形成及び木材組織構造の網羅的解析から迫る木本植物の進化と環境適応性
  自 2022年, 至 2022年
• 基盤研究(B)
  13CO2パルスラベリングによる樹幹内炭素配分過程の解明と肥大成長モデルの構築
  自 2022年, 至 2022年
• 基盤研究(B)
  樹木の細胞壁三次元構造の多様性発現機構のイメージング解析
  自 2021年, 至 2021年
• 基盤研究(B)
  イチョウの材形成及び木材組織構造の網羅的解析から迫る木本植物の進化と環境適応性
  自 2021年, 至 2021年
• 基盤研究(B)
  樹木の土壌病害の早期発見手法の開発
  自 2021年, 至 2021年
• 基盤研究(B)
  ときに何十年も生存する樹木木部柔細胞の生活史―幹放射方向を区分する新基準の提案
  自 2021年, 至 2021年
• 基盤研究(B)
  13CO2パルスラベリングによる樹幹内炭素配分過程の解明と肥大成長モデルの構築
  自 2021年, 至 2021年
• 挑戦的研究（萌芽）
  細胞老化の視点から切り拓く樹木の心材形成研究の新展開
  自 2020年, 至 2023年
• 挑戦的研究（萌芽）
  樹木肥大成長期間の検証：年輪は１年のうちに完成しているのか？
  自 2020年, 至 2020年
• 基盤研究(B)
  13CO2パルスラベリングによる樹幹内炭素配分過程の解明と肥大成長モデルの構築
  自 2020年, 至 2020年
• 基盤研究(B)
  立木の幹内部を可視化する手法を用いた樹幹師部―木部の放射方向の物質移動機構の解明
  自 2020年, 至 2020年
• 基盤研究(B)
  樹木細胞壁の階層構造構築機構の直接生体イメージング解析
  自 2020年, 至 2020年
• 基盤研究(B)
  心材形成機構に迫る新規アプローチ：細胞死を制御する自己分解酵素の時空間動態の解明
  自 2019年, 至 2021年
• 挑戦的研究（萌芽）
  樹木肥大成長期間の検証：年輪は１年のうちに完成しているのか？
  自 2019年, 至 2019年
• 基盤研究(B)
  立木の幹内部を可視化する手法を用いた樹幹師部―木部の放射方向の物質移動機構の解明
  自 2019年, 至 2019年
• 基盤研究(B)
  樹木細胞壁の階層構造構築機構の直接生体イメージング解析
  自 2019年, 至 2019年
• 基盤研究(B)
  立木の幹内部を可視化する手法を用いた樹幹師部―木部の放射方向の物質移動機構の解明
  自 2018年, 至 2018年
• 挑戦的研究（萌芽）
  樹木肥大成長期間の検証：年輪は１年のうちに完成しているのか？
  自 2018年, 至 2018年
• 基盤研究(B)
  樹木細胞壁の階層構造構築機構の直接生体イメージング解析
  自 2018年, 至 2018年
• 基盤研究(B)
  樹木の細胞壁高次構造形成機構のリアルタイムイメージング解析
  自 2017年, 至 2017年
• 基盤研究(B)
  樹木の細胞壁高次構造形成機構のリアルタイムイメージング解析
  自 2016年, 至 2016年
• 基盤研究(C)
  樹木の心材形成を制御する自己分解酵素の挙動に関する細胞生物学的研究
  自 2015年, 至 2017年
• 基盤研究(B)
  樹木の細胞壁高次構造形成機構のリアルタイムイメージング解析
  自 2015年, 至 2015年
• 基盤研究（Ｂ）
  樹木の二次木部細胞の分化および細胞死制御機構の直接イメージング解析
  自 2014年, 至 2014年
• 若手研究(B)
  心材形成誘導系を用いた樹木独自の細胞死発現機構の解明
  自 2013年, 至 2014年
• 基盤研究(B)
  組織化学と樹幹の振動現象による心材形成過程のモニタリング
  自 2013年, 至 2013年
• 基盤研究(B)
  樹木の二次木部細胞の分化および細胞死制御機構の直接イメージング解析
  自 2013年, 至 2013年
• 研究活動スタート支援
  樹木の長命細胞をモデルとした新規細胞死発現機構の解明
  自 2012年, 至 2013年
• 基盤研究(B)
  組織化学と樹幹の振動現象による心材形成過程のモニタリング
  自 2012年, 至 2012年

論文

• Relationship between the amount of black carbon particles deposited on the leaf surface and leaf surface traits in nine urban greening tree species
  Ohta, Akari; Takahashi, Kei; Sase, Hiroyuki; Murao, Naoto; Takada, Keiichi; Yamaguchi, Masahiro; Murakami, Hisashi; Nakaba, Satoshi; Watanabe, Makoto; Mizukawa, Kaoruko; Takada, Hideshige; Izuta, Takeshi
  INTERNATIONAL JOURNAL OF PHYTOREMEDIATION
  TAYLOR & FRANCIS INC
  To select urban greening tree species suitable for the purification of the atmosphere polluted by black carbon (BC) particles, it is necessary to clarify the determinants of the amount of BC particles deposited on the tree leaves. In the present study, we investigated the relationship between the amount of BC particles that were deposited from the atmosphere and firmly adhered to the leaf epicuticular wax, and leaf surface traits in seedlings of nine tree species grown for two years under natural conditions (Fuchu, Tokyo, Japan). There was a significant interspecific difference in the maximum amount of BC particles deposited on the leaf surface, and the order was as follows: Ilex rotunda > Cornus florida > Osmanthus fragrans > Cornus kousa > Quercus glauca approximately equal to or the image of Quercus myrsinifolia > Magnolia kobus approximately equal to or the image of Zelkova serrata approximately equal to or the image of Styrax japonicus. In the nine tree species, significant highly positive correlations were observed between the amount of BC particles deposited on the leaf surface, and the hydrophobicity of leaf epicuticular wax determined by its chemical composition. Therefore, we concluded that the hydrophobicity of leaf epicuticular wax is an important determinant of the amount of BC particles deposited on the leaf surface of urban greening tree species. NOVELTY STATEMENTThis is the first paper that shows that the hydrophobicity of leaf epicuticular wax is an important determinant of the amount of BC particles deposited on the leaf surface of urban greening tree species. This study will provide the basic and novel information for the selection of urban greening tree species suitable for the purification of the air polluted by BC particles.
  2023年08月24日, 研究論文（学術雑誌）, 共同, 25, 10, 1522-6514, DOI(公開)(r-map), 1384, 1396
• Anatomical and compressive characterization of Cryptomeria japonica hydrolyzed with phosphoric acid for lignin residue utilization
  Kurei, Tatsuki; Hirano, Seiya; Nakaba, Satoshi; Funada, Ryo; Horikawa, Yoshiki
  JOURNAL OF MATERIALS SCIENCE
  SPRINGER
  The handling of lignin residues is a concern for the use of wood in biorefineries. This study proposes a new pathway for lignin residues that uses the structure of wood. Optimal phosphoric acid hydrolysis to remove polysaccharides was selected from the principal component analysis based on infrared spectra. Acid-hydrolyzed wood, the lignin residue, exhibited anisotropic shrinkage, but its anatomical structure was not fractured. The strength and modulus of the lignin residue varied with the compression direction, reflecting the anisotropic structure of the wood that was not fractured by acid hydrolysis. The density of the lignin residue initially decreased and then slightly increased during ten cycles of 5 wt% phosphoric acid hydrolysis at 180 degrees C for 1 h, resulting in minor recovery of the modulus and strength. Therefore, practical durability can be achieved by increasing the density of the lignin residue through densification or combination with other functional polymers. Thus, simpler top-down strategies are expected compared with the currently proposed bottom-up approach for lignin. [GRAPHICS] .
  2023年07月15日, 研究論文（学術雑誌）, 共同, 0022-2461, DOI(公開)(r-map)
• Elucidation of alcoholysis for the preparation of lignin-free wood sections from Cryptomeria japonica
  Hirano, Seiya; Kurei, Tatsuki; Nakaba, Satoshi; Funada, Ryo; Horikawa, Yoshiki
  CELLULOSE
  SPRINGER
  This study reports on the preparation of lignin-free wood sections maintaining the anatomical structure of Cryptomeria japonica by means of alcoholysis combined with sodium chlorite treatment. To determine the optimal alcoholysis conditions, Fourier transform infrared spectroscopy was combined with multivariate analysis, and the obtained results indicated that the alcoholysis reaction proceeded over three stages. In the first stage, lignin and hemicellulose are partially removed. In the second stage, hemicellulose and amorphous cellulose are degraded and humin is formed. In the third stage, crystalline cellulose is degraded, further promoting the formation of humin. Since it is difficult to completely remove lignin by alcoholysis alone, the wood sections were subsequently subjected to sodium chlorite treatment. As a result, lignin-free sections were produced without degradation of the woody anatomical structure. Furthermore, alcoholysis at 140 degrees C for 1 h coupled with sodium chlorite treatment for 1 h succeeded in producing sections composed of essentially hemicellulose-free cellulose. These achievements make this protocol potentially applicable as a substrate for artificial cell walls and for the development of novel functional filters.
  2023年07月, 研究論文（学術雑誌）, 共同, 30, 10, 0969-0239, DOI(公開)(r-map), 6589, 6600
• Seasonal variations in the amount of black carbon particles deposited on the leaf surfaces of nine Japanese urban greening tree species and their related factors
  Takahashi, Kei; Ohta, Akari; Sase, Hiroyuki; Murao, Naoto; Takada, Keiichi; Yamaguchi, Masahiro; Nakaba, Satoshi; Watanabe, Makoto; Izuta, Takeshi
  INTERNATIONAL JOURNAL OF PHYTOREMEDIATION
  TAYLOR & FRANCIS INC
  NOVELTY STATEMENT This is the first paper that reports the seasonal variations in the amount of BC particles deposited on the leaves of Japanese urban greening tree species and their related factors such as environmental conditions and leaf surface characteristics. This study will provide the basic and novel information for the phytoremediation of urban air pollution induced by BC particles in Asia. As black carbon (BC) particles can be deposited on the leaf surfaces, urban greening is considered to be effective in purifying urban air. However, little information on the seasonal variations in the amount of BC particles deposited on the leaf surfaces (BC amount on the leaves) is available in Japanese urban greening tree species. Therefore, we investigated seasonal variations in the BC amount on the leaves of evergreen (Quercus glauca, Quercus myrsinaefolia, Osmanthus fragrans and Ilex rotunda) and deciduous (Zelkova serrata, Styrax japonica, Magnolia kobus, Cornus kousa and Cornus florida) broad-leaved tree species. The BC amount on the leaves tended to increase from April for different periods, and then reached a saturated state in the tree species, excluding M. kobus. In the 4 evergreen broad-leaved trees, the seasonal variation was positively correlated with the atmospheric concentration of BC particle. In the 5 deciduous broad-leaved trees, the seasonal variation was negatively and positively correlated with the water-repellence (water droplet contact angle) and the amount of epicuticular wax on the leaf surface, respectively. Therefore, the BC amounts on the leaves of evergreen and deciduous broad-leaved urban tree species are considered to be mainly regulated by environmental factors and leaf surface characteristics, respectively.
  2023年01月28日, 研究論文（学術雑誌）, 共同, 25, 2, 1522-6514, DOI(公開)(r-map), 252, 262
• Sequent periderm formation and changes in the cellular contents of phloem parenchyma during rhytidome development in Cryptomeria japonica
  Ohse, Megumi; Irohara, Rika; Iizuka, Etsushi; Arakawa, Izumi; Kitin, Peter; Funada, Ryo; Nakaba, Satoshi
  JOURNAL OF WOOD SCIENCE
  SPRINGER JAPAN KK
  The outer bark that includes sequent periderms is referred to as rhytidome. The defense and physiological functions of rhytidome are maintained by the continuous formation of sequent periderms. To understand the mechanisms of rhytidome growth, we examined the development of sequent periderms and the corresponding changes in the cellular contents of phloem parenchyma cells in Cryptomeria japonica. New layers of rhytidome were formed in the studied trees during the two-year course of the study. Our records showed that a new layer of periderm forms annually, and therefore, rhytidome development in C. japonica can be studied by sequential sample collection in any given year. Formation of new periderm and initiation of nuclei disappearance in phloem parenchyma in the outer layers of the developing outer bark occurred simultaneously. The early disappearance of nuclei indicates that some parenchyma cells might have been in a stage of preparation for cell death before the formation of new periderm. Four developmental stages of annual rhytidome growth were identified by structural and physiological changes of the outer layers of phloem parenchyma and the growth of the new periderm.
  2022年12月, 研究論文（学術雑誌）, 共同, 68, 1, 1435-0211, DOI(公開)(r-map)
• The effects of watering on cambial activity in the stems of evergreen hardwood (Samanea saman) during the pre-monsoon season in subtropical Bangladesh
  Rahman, Md Hasnat; Begum, Shahanara; Nugroho, Widyanto Dwi; Nakaba, Satoshi; Funada, Ryo
  JOURNAL OF WOOD SCIENCE
  SPRINGER JAPAN KK
  Water stress has a significant impact on tree growth. However, the effects of watering on cambial activity and its influence on tree growth in subtropical climates is poorly understood. The present study analyzed the cambial activity on the stem of evergreen hardwood Samanea saman in response to either high frequency or low frequency watering during the pre-monsoon season in subtropical Bangladesh. We used two groups of seedlings: one group of seedlings was watered daily (high frequency watering), while the second group of seedlings was watered at 4-5-day intervals (low frequency watering). Samples for sequential observations of cambial activity by microscopy were collected from the main stems of seedlings of both groups. At the start of the experiment on March 25, 2015, during the pre-monsoon season, the cambium was inactive with no evidence of cell division. After 10 days of high frequency watering, cambial cell division and xylem differentiation were initiated. New cell plates were formed in the phloem side of the cambium. However, the cambium was inactive when low frequency watering was supplied. Supplying water in high frequency reactivated the cambium with forming small to large vessels. In contrast, the cambium remained inactive when low frequency watering was supplied throughout the experiment. These results suggest that continuous supply of water to the soil is one of the most important factors for cambial reactivation during pre-monsoon season in subtropical trees. Furthermore, our findings of artificial watering treatments might help to better understand the response of cambium to changes in precipitation patterns under natural conditions, allowing us to learn more about how cambium of subtropical trees responds to climate change.
  2022年12月, 研究論文（学術雑誌）, 共同, 68, 1, 1435-0211, DOI(公開)(r-map)
• A combination of scanning electron microscopy and broad argon ion beam milling provides intact structure of secondary tissues in woody plants
  Hatano, Tomohiro; Nakaba, Satoshi; Horikawa, Yoshiki; Funada, Ryo
  SCIENTIFIC REPORTS
  NATURE PORTFOLIO
  The secondary tissues of woody plants consist of fragile cells and rigid cell walls. However, the structures are easily damaged during mechanical cross-sectioning for electron microscopy analysis. Broad argon ion beam (BIB) milling is commonly employed for scanning electron microscopy (SEM) of hard materials to generate a large and distortion-free cross-section. However, BIB milling has rarely been used in plant science. In the present study, SEM combined with BIB milling was validated as an accurate tool for structural observation of secondary woody tissues of two samples, living pine (Pinus densiflora) and high-density oak wood (Quercus phillyraeoides), and compared with classical microtome cross-sectioning. The BIB milling method does not require epoxy resin embedding because of prior chemical fixation and critical point drying of the sample, thus producing a three-dimensional image. The results showed that xylem structures were well-preserved in their natural state in the BIB-milled cross-section compared with the microtome cross-section. The observations using SEM combined with BIB milling were useful for wide-area imaging of both hard and soft plant tissues, which are difficult to observe with transmitted electron microscopy because it is difficult to obtain sections of such tissues, particularly those of fragile reaction woods.
  2022年06月01日, 研究論文（学術雑誌）, 共同, 12, 1, 2045-2322, DOI(公開)(r-map)
• Effects of orientation and degree of polymerization on tensile properties in the cellulose sheets using hierarchical structure of wood
  Kurei, Tatsuki; Hioki, Yuto; Kose, Ryota; Nakaba, Satoshi; Funada, Ryo; Horikawa, Yoshiki
  CELLULOSE
  SPRINGER
  Structural control is essential for further development of cellulosic materials. Here, we demonstrated the significance of the orientation and degree of polymerization in the integrated structure of cellulose microfibrils using sheets based on the hierarchical structure of wood. Non-cellulosic components were removed from wood blocks of a conifer by applying a two-step chemical treatment. Partially changing the treatment conditions generated cellulose blocks with varying degrees of polymerization while maintaining the anatomical structure. Cellulose sheets with oriented microfibrils were subsequently prepared by heat-pressing the blocks. Sheets with randomized appearance of orientation were also produced by disassembling tracheid alignment. These sheets were then subjected to structural evaluation and tensile tests. Comparison of both sheets showed that microfibril orientation mainly determined the modulus. As long as the orientation was maintained, the specific modulus was independent of the degree of polymerization. In contrast, the tensile strength of the oriented sheet varied with the degree of polymerization, indicating that it notably reflected the single fiber strength compared to the randomly oriented sheet. Hence, a highly oriented structure with unfragmented microfibrils is the determinant of a superior material. Consequently, the sheets in which these parameters are readily controlled can advance cellulose applications.
  2022年03月, 研究論文（学術雑誌）, 共同, 29, 5, 0969-0239, DOI(公開)(r-map), 2885, 2898
• オオヤマレンゲの未成熟種子を用いた組織培養による植物体再生に関する研究
  Mori, Junya; Tsukada, Kentaro; Inoue, Ryohei; Nozawa, Yoko; Yamagishi, Yusuke; Yasue, Koh; Nakaba, Satoshi; Funada, Ryo
  MOKUZAI GAKKAISHI
  JAPAN WOOD RES SOC
  Magnolia sieboldii subsp. japonica(Oyama renge)is a deciduous tree that is native to Japan. This tree has value as a rarity because it is designated as an endangered species in some prefectures and, therefore, its conservation and reproduction are very important. We initially cultured immature seeds on Lepoivre medium(LP), half of nitrogen source of Murashige and Skoog medium(1/2N MS)and Woody Plant Medium(WPM) containing a-naphthaleneacetic acid (NAA) as an auxin and 6-benzylaminopurine(BAP)as a cytokinin. Proembryogenic masses(PEMs)were induced directly from immature seeds and somatic embryos were induced from PEMs. Immature seeds one to three weeks after flowering might be suitable to induce PEMs. Some somatic embryos formed roots and hypocotyls under condition of lighting. Finally, we transferred the somatic embryo-derived plantlets to soil in pots and the plantlets continued to grow. Thus, we developed a tissue culture system for plant regeneration for the propagation of M. sieboldii subsp. japonica.
  2022年, 研究論文（学術雑誌）, 共同, 68, 4, 0021-4795, DOI(公開)(r-map), 145, 153
• Influence of soil properties on the heartwood colour of Juglans mandshurica var. sachalinensis in a cool temperate forest
  Makoto, Kobayashi; Susloparova, Elizaveta; Tsuyama, Ikutaro; Shimase, Takuya; Nakaba, Satoshi; Takahashi, Naoki; Yoshida, Toshiya
  JOURNAL OF WOOD SCIENCE
  SPRINGER JAPAN KK
  Heartwood colour is often an important factor in determining timber prices. However, the determinants of intraspecific variation in heartwood colour, which is useful information for sustainable wood marketing, are little understood, especially at the local scale in cool temperate forests. Because heartwood is produced as a secondary compound and photosynthesis is regulated by nitrogen (N) in cool temperate forests, we hypothesized that (1) soil conditions determine heartwood colour even at a local scale within a tree species and (2) N, specifically, can be an important driver of the intraspecific variation in heartwood colour in the trees of cool temperate forests. To test these hypotheses, we investigated the relationship between the colour values (luminescence, redness, and yellowness) of heartwood from Juglans mandshurica var. sachalinensis and the soil parameters in a cool temperate forest. Among the soil properties, not soil N but soil magnesium (Mg) contents alone had a significant influence on the redness and yellowness of the heartwood. Higher soil Mg contents resulted in increased redness and yellowness of the heartwood in our study, probably due to the increase in phenolics and the colouring of the tannins in the heartwood with Mg. Our results indicate that even at a local scale, soil condition can determine the intraspecific variation in heartwood colour and that forest managers can utilize edaphic information to predict heartwood colour for timber marketing.
  2021年12月, 研究論文（学術雑誌）, 共同, 67, 1, 1435-0211, DOI(公開)(r-map)
• Spatial and temporal patterns of wound periderm development in Cryptomeria japonica bark
  Iizuka, Etsushi; Ohse, Megumi; Arakawa, Izumi; Kitin, Peter; Funada, Ryo; Nakaba, Satoshi
  IAWA JOURNAL
  BRILL
  Limited investigations have been carried out on the physiological and growth responses of bark to wounding, even though wound periderms play crucial roles in tree defenses. To understand the mechanisms of wound periderm formation, we studied the growth responses and structural changes of wounded bark of three Cryptomeria japonica individuals. We observed the developmental time frame and morphology of wound periderms around mechanically induced wounds in summer. The wound responses included discoloration, lignification, and suberization in tissues present at the time of wounding, followed by wound periderm formation and secondary metabolite deposition. The trees had developed wound periderms approximately 4 weeks after wounding. The wound periderms were within 3 mm in the axial directions and within 1 mm in the lateral directions from the wound surfaces. The distinct patterns of wound periderm formation in the axial and lateral regions resulted from the arrangement and anatomical features of the cells adjacent to the wounds. The wound phellem cells were tangentially narrower and axially shorter in the side and upper/lower regions, respectively, of the wounds. Therefore, the cell division frequencies in the planes parallel to the wound surface may be greater than those in the other directions. Wound reactions in bark might initially be triggered by microenvironmental changes, such as the spread of desiccation, which depends directly on the morphology of phloem cell complexes.
  2021年11月, 研究論文（学術雑誌）, 共同, 42, 4, 0928-1541, DOI(公開)(r-map), 486, 496
• Light microscopy of wood using sanded surface instead of slides
  Kitin, Peter; Hermanson, John C.; Abe, Hisashi; Nakaba, Satoshi; Funada, Ryo
  IAWA JOURNAL
  BRILL
  Xylem anatomy is fundamental in studies of the evolution of terrestrial plants, tree ecophysiology, forestry, and wood science. Traditional xylem anatomical studies by light microscopy utilize wood sections. However, the procedures are laborious, and high-quality histological sections have been particularly challenging to achieve from recalcitrant wood species and dry wood material. Modern microscopy offers opportunities for speeding up the xylem anatomical preparations. In this regard, the merits of using a sanded surface for wood anatomical research have been largely overlooked. Sanding of wood surfaces is practiced in dendrochronology and wood identification studies exclusively for the investigation of macro features, such as tree rings, wood porosity, or parenchyma patterns. We conducted microscopic level investigations of sanded surfaces of difficult-to-section high-density woods such as Dalbergia and Quercus species by reflected white light and epifluorescence microscopy. Reflected white light or combinations of reflected light and fluorescence could clearly show xylem micro-features in sanded wood surfaces. The resolution of cell types after sanding with 1000-grit was similar to the resolution obtained by transmitted light microscopy in histological slides. The advantages of sanded wood surfaces compared to traditional wood sections can be summarized as cost-and time-effective sample preparation, large sample area, intact cell walls and tissue structure, preservation of chemical content and extractives, and even focus of the field of view. A simple procedure of wood sanding instead of microscopic slides can be used for xylem microscopy and automatic image analysis of xylem structure.
  2021年07月, 研究論文（学術雑誌）, 共同, 42, 3, 0928-1541, DOI(公開)(r-map), 322, 335
• Tracheary elements from calli of Japanese horse chestnut (Aesculus turbinata) form perforation-like structures
  Yamagishi, Yusuke; Kudo, Kayo; Yoshimoto, Joto; Nakaba, Satoshi; Nabeshima, Eri; Watanabe, Ugai; Funada, Ryo
  PLANTA
  SPRINGER
  Main conclusion Calli derived from young leaves of Aesculus turbinata contained tracheary elements with large pores that resembled perforations of vessel elements. The differentiation of tracheary elements in vitro provides a useful system for detailed analysis of xylem cell differentiation. To examine the mechanism of formation of cell wall structures, new differentiation systems are required that allows us to induce highly organized structures, such as perforations. In this study, we developed such a system in which we were able to induce formation of tracheary elements with perforations, using calli of a hardwood, Aesculus turbinata. Young leaves of A. turbinata were placed on modified MS medium that contained 5 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) and 5 mu M benzyladenine (BA). Tracheary elements were induced in calli derived from young leaves of A. turbinata. Some tracheary elements formed broad areas of secondary wall with typical features of secondary xylem. Other tracheary elements formed spiral thickenings, which are typical features of vessel elements in secondary xylem of A. turbinata. Approximately 10% of tracheary elements formed large pores that resembled perforations of vessel elements and various types of the perforation plate were observed. Addition of NAA and brassinolide to the induction medium enhanced the differentiation of tracheary elements in calli of A. turbinata. Newly induced tracheary elements also formed typical features of secondary xylem such as perforations of the vessel elements. Our model system might be useful in efforts to understand the mechanisms of formation of highly organized structures in tracheary elements in secondary xylem.
  2021年05月, 研究論文（学術雑誌）, 共同, 253, 5, 0032-0935, DOI(公開)(r-map)
• Creation and structural evaluation of the three-dimensional cellulosic material White-Colored Bamboo
  Kurei, Tatsuki; Tsushima, Rino; Okahisa, Yoko; Nakaba, Satoshi; Funada, Ryo; Horikawa, Yoshiki
  HOLZFORSCHUNG
  WALTER DE GRUYTER GMBH
  This study reports a new cellulosic material that maintains the original three-dimensional structure of bamboo and is produced by optimization of chemical treatments. Bamboo blocks were prepared from the current year culms of moso bamboo (Phyllostachys edulis) and subjected to three chemical treatments alone or in combination. Based on the color changes and Fourier transform infrared spectra, the combination of alkaline treatment with alcoholysis followed by Wise method was found to be an optimal treatment method. This serial treatment caused the blocks to become completely white and removed non-cellulosic components such as hemicellulose and lignin from the cell walls of the parenchyma as well as those of vascular bundles. This sample was named as White-Colored Bamboo. Extensive structural evaluations from anatomical- to nano- level were performed using X-ray computed tomography, X-ray diffraction, and transmission electron microscopy incorporated with the 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation technique. These multiple observations showed that the white-colored bamboo maintained its inherent hierarchical structure, thus encouraging to produce functional biomaterials.
  2021年02月, 研究論文（学術雑誌）, 共同, 75, 2, 0018-3830, DOI(公開)(r-map), 180, 186
• Winter-spring temperature pattern is closely related to the onset of cambial reactivation in stems of the evergreen conifer Chamaecyparis pisifera
  Rahman, Md Hasnat; Kudo, Kayo; Yamagishi, Yusuke; Nakamura, Yusuke; Nakaba, Satoshi; Begum, Shahanara; Nugroho, Widyanto Dwi; Arakawa, Izumi; Kitin, Peter; Funada, Ryo
  SCIENTIFIC REPORTS
  NATURE PUBLISHING GROUP
  Temperature is an important factor for the cambial growth in temperate trees. We investigated the way daily temperatures patterns (maximum, average and minimum) from late winter to early spring affected the timing of cambial reactivation and xylem differentiation in stems of the conifer Chamaecyparis pisifera. When the daily temperatures started to increase earlier from late winter to early spring, cambial reactivation occurred earlier. Cambium became active when it achieves the desired accumulated temperature above the threshold (cambial reactivation index; CRI) of 13 degrees C in 11 days in 2013 whereas 18 days in 2014. This difference in duration required for achieving accumulated temperature can be explained with the variations in the daily temperature patterns in 2013 and 2014. Our formula for calculation of CRI predicted the cambial reactivation in 2015. A hypothetical increase of 1-4 degrees C to the actual daily maximum temperatures of 2013 and 2014 shifted the timing of cambial reactivation and had different effects on cambial reactivation in the two consecutive years because of variations in the actual daily temperatures patterns. Thus, the specific annual pattern of accumulation of temperature from late winter to early spring is a critical factor in determining the timing of cambial reactivation in trees.
  2020年08月31日, 研究論文（学術雑誌）, 共同, 10, 1, 2045-2322, DOI(公開)(r-map)
• Pathways of extra- and intercellular diffusion of colored substances in the blackened xylem ofDiospyros kaki
  Iwami, Kana; Noma, Satoshi; Ban, Takuya; Matsushita, Yasuyuki; Arakawa, Izumi; Kitin, Peter; Funada, Ryo; Nakaba, Satoshi
  JOURNAL OF WOOD SCIENCE
  SPRINGER JAPAN KK
  Some species ofDiospyrosform the black wood that is known as ebony. Infrequently,D.kakiforms wood with black patterning that is called kurogaki in Japan and is highly valued. To understand the mechanism of formation of the blackened xylem, we attempted to clarify the pathways of diffusion of colored substances from the site of their biosynthesis to their deposition in secondary xylem. We observed colored substances in pits and intercellular spaces and we recorded the deposition of colored substances in the cell walls of wood fibers. In gray and black regions, we found colored substances in the pits between xylem cells, in blind pits and in intercellular spaces. At the boundary between whitish and black regions, areas of coloration in the cell walls of wood fibers gradually increased in size from the whitish to the black regions. Heavy deposition of colored substances was observed in the inner region of the secondary walls of wood fibers. Furthermore, coloration of the outer region of the secondary walls and compound middle lamella (CML) of wood fibers gradually became stronger toward the black regions. Our observations suggest that pit-pairs and intercellular spaces might provide the pathways for inter- and extracellular diffusion of colored substances, respectively. In addition, colored substances might penetrate the cell walls of wood fibers, moving from the lumen into the cell wall.
  2020年07月03日, 研究論文（学術雑誌）, 共同, 66, 1, 1435-0211, DOI(公開)(r-map)
• Direct fluorescence imaging of lignocellulosic and suberized cell walls in roots and stems
  Kitin, Peter; Nakaba, Satoshi; Hunt, Christopher G.; Lim, Sierin; Funada, Ryo
  AOB PLANTS
  OXFORD UNIV PRESS
  Investigating plant structure is fundamental in botanical science and provides crucial knowledge for the theories of plant evolution, ecophysiology and for the biotechnological practices. Modern plant anatomy often targets the formation, localization and characterization of cellulosic, lignified or suberized cell walls. While classical methods developed in the 1960s are still popular, recent innovations in tissue preparation, fluorescence staining and microscopy equipment offer advantages to the traditional practices for investigation of the complex lignocellulosic walls. Our goal is to enhance the productivity and quality of microscopy work by focusing on quick and cost-effective preparation of thick sections or plant specimen surfaces and efficient use of direct fluorescent stains. We discuss popular histochemical microscopy techniques for visualization of cell walls, such as autofluorescence or staining with calcofluor, Congo red (CR), fluorol yellow (FY) and safranin, and provide detailed descriptions of our own approaches and protocols. Autofluorescence of lignin in combination with CR and FY staining can clearly differentiate between lignified, suberized and unlignified cell walls in root and stem tissues. Glycerol can serve as an effective clearing medium as well as the carrier of FY for staining of suberin and lipids allowing for observation of thick histological preparations. Three-dimensional (3D) imaging of all cell types together with chemical information by wide-field fluorescence or confocal laser scanning microscopy (CLSM) was achieved.
  2020年06月29日, 研究論文（学術雑誌）, 共同, 12, 4, 2041-2851, DOI(公開)(r-map)
• Development of colorless wood via two-step delignification involving alcoholysis and bleaching with maintaining natural hierarchical structure
  Horikawa, Yoshiki; Tsushima, Rino; Noguchi, Keiichi; Nakaba, Satoshi; Funada, Ryo
  JOURNAL OF WOOD SCIENCE
  SPRINGER JAPAN KK
  Lignin-free wood has been successfully developed via a two-step chemical treatment while maintaining its inherent hierarchical structure. The first step was alcoholysis which was conducted using ethylene glycol, and whose condition was optimized by monitoring the removal of lignin using infrared spectroscopy. The second step was bleaching wherein the delignification proceeded from the surface to the core of the wood block, and finally resulted in complete decolorization. Although the wood block was free from lignin and hemicellulose as approximately confirmed by the chemical composition analysis, the 3-dimensional colorless wood block was almost unaltered, even after freeze-drying. Then, multidirectional observation was performed to investigate whether the natural hierarchical structure from anatomical- to nano-level was maintained. Optical microscopy, X-ray microcomputed tomography, X-ray diffractometry, and transmission electron microscopy demonstrated that all the stages of hierarchical structure were maintained. The lignin-free wood block has great potential for novel materials that are supported by a 3-dimensional wooden architecture. The derived lignin-free wood is also a suitable specimen that can be used to understand the formation and functionality of the anatomical structure and lignified cell wall.
  2020年05月19日, 研究論文（学術雑誌）, 共同, 66, 1, 1435-0211, DOI(公開)(r-map)
• Artificially lignified cell wall catalyzed by peroxidase selectively localized on a network of microfibrils from cultured cells
  Hirano, Seiya; Yamagishi, Yusuke; Nakaba, Satoshi; Kajita, Shinya; Funada, Ryo; Horikawa, Yoshiki
  PLANTA
  SPRINGER
  Main conclusion An artificial lignified cell wall was synthesized in three steps: (1) isolation of microfibrillar network; (2) localization of peroxidase through immunoreaction; and (3) polymerization of DHP to lignify the cell wall. Artificial woody cell wall synthesis was performed following the three steps along with the actual formation in nature using cellulose microfibrils extracted from callus derived from Cryptomeria japonica. First, we constructed a polysaccharide network on a transmission electron microscopy (TEM) grid. The preparation method was optimized by chemical treatment, followed by mechanical fibrillation to create a microfibrillated network. Morphology was examined by TEM, and chemical characterization was by Fourier transform infrared (FTIR) spectroscopy. Second, we optimized the process to place peroxidase on the microfibrils via an immunoreaction technique. Using a xyloglucan antibody, we could ensure that gold particles attached to the secondary antibodies were widely and uniformly localized along with the microfibril network. Third, we applied the peroxidase attached to secondary antibodies and started to polymerize the lignin on the grid by simultaneously adding coniferyl alcohol and hydrogen peroxide. After 30 min of artificial lignification, TEM observation showed that lignin-like substances were deposited on the polysaccharide network. In addition, FTIR spectra revealed that the bands specific for lignin had increased, demonstrating the successful artificial formation of woody cell walls. This approach may be useful for studying woody cell wall formation and for producing made-to-order biomaterials.
  2020年05月07日, 研究論文（学術雑誌）, 共同, 251, 6, 0032-0935, DOI(公開)(r-map)
• Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein
  Murakami, Tomoaki; Noguchi, Keiichi; Hachiya, Naomi; Kametani, Fuyuki; Tasaki, Masayoshi; Nakaba, Satoshi; Sassa, Yukiko; Yamashita, Taro; Obayashi, Konen; Ando, Yukio; Hamamura, Masao; Kanno, Takeshi; Kawasako, Kazufumi
  AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
  TAYLOR & FRANCIS LTD
  Amyloidosis is an extremely rare event in rats. In this study, we report that lipopolysaccharide binding protein (LBP) is the most likely amyloidogenic protein in rat mammary amyloidosis. Histologically, corpora amylacea (CA) and stromal amyloid (SA) were observed in rat mammary glands, and needle-shaped amyloid (NA) was also observed on the surface or gap of CA and SA. Following surveillance in aged rats, NA was observed in 62% of mammary tumours, 25% of male mammary glands and 83% of female mammary glands. Proteomic analysis showed that lactadherin was a major constitutive protein of CA and SA, and both were positive following immunohistochemistry with anti-lactadherin antibodies. In the same analysis, LBP was detected as a prime candidate protein in NA, and NA was positive following immunohistochemistry and immunoelectron microscopy with anti-LBP antibody. Furthermore, synthetic peptides derived from rat LBP formed amyloid fibrils in vitro. Overall, these results provide evidence that LBP is an amyloid precursor protein of NA in rat mammary glands.
  2020年01月02日, 研究論文（学術雑誌）, 共同, 27, 1, 1350-6129, DOI(公開)(r-map), 25, 35
• Visualization and Localization of Submicron-Sized Ammonium Sulfate Particles on Needles of Japanese Larch (Larix kaempferi) and Japanese Cedar (Cryptomeria japonica) and Leaves of Japanese Beech (Fagus crenata) and Japanese Chinquapin (Castanopsis sieboldii) after Artificial Exposure
  Yamane, Kenichi; Nakaba, Satoshi; Yamaguchi, Masahiro; Kuroda, Katsushi; Sano, Yuzou; Lenggoro, I. Wuled; Izuta, Takeshi; Funada, Ryo
  FORESTS
  MDPI
  We applied a method combining field-emission scanning electron microscopy (FE-SEM) with energy dispersive X-ray spectrometry (EDX) to visualize the deposition and localization of the submicron-sized ammonium sulfate (AS) particles. The AS particles emitted from an aerosol generator in the laboratory were spherical in shape and individually deposited without aggregation on the surface of a silicon substrate. We determined the AS particles on the surfaces of the needles of Japanese larch (Larix kaempferi) and Japanese cedar (Cryptomeria japonica), and the leaves of Japanese beech (Fagus crenata) and Japanese chinquapin (Castanopsis sieboldii), using EDX. The particles were deposited on either the adaxial or abaxial side of the leaves and needles. The AS particles deposited on the surfaces of the leaves and needles did not aggregate, and they were deposited on the surfaces of the leaves and needles in the same manner, regardless of leaf structure. These results, using a new method, highlight the early stages of the deposition and localization of submicron-sized AS particles on the surfaces of the leaves and needles of forest trees.
  2019年12月, 研究論文（学術雑誌）, 共同, 10, 12, DOI(公開)(r-map)
• Localization of actin filaments and cortical microtubules in wood-forming tissues of conifers
  Begum, Shahanara; Furusawa, Osamu; Shibagaki, Masaki; Nakaba, Satoshi; Yamagishi, Yusuke; Yoshimoto, Joto; Rahman, Md Hasnat; Sano, Yuzou; Funada, Ryo
  IAWA JOURNAL
  BRILL ACADEMIC PUBLISHERS
  The aim of the present study was to investigate the orientation and localization of actin filaments and cortical microtubules in wood-forming tissues in conifers to understand wood formation. Small blocks were collected from the main stems of Abies firma, Pinus densiflora, and Taxus cuspidata during active seasons of the cambium. Bundles of actin filaments were oriented axially or longitudinally relative to the cell axis in fusiform and ray cambial cells. In differentiating tracheids, actin filaments were oriented longitudinally relative to the cell axis during primary and secondary wall formation. In contrast, the orientation of well-ordered cortical microtubules in tracheids changed from transverse to longitudinal during secondary wall formation. There was no clear relationship between the orientation of actin filaments and cortical microtubules in cambial cells and cambial derivatives. Aggregates of actin filaments and a circular band of cortical microtubules were localized around bordered pits and cross-field pits in differentiating tracheids. In addition, rope-like bands of actin filaments were observed during the formation of helical thickenings at the final stage of formation of secondary walls in tracheids. Actin filaments might not play a major role in changes in the orientation of cortical microtubules in woodforming tissues. However, since actin filaments were co-localized with cortical microtubules during the formation of bordered pits, cross-field pits and helical thickenings at the final stage of formation of the secondary wall in tracheids, it seems plausible that actin filaments might be closely related to the localization of cortical microtubules during the development of these modifications of wood structure.
  2019年11月, 研究論文（学術雑誌）, 共同, 40, 4, 0928-1541, DOI(公開)(r-map), 703, 720
• ウルシの未成熟胚を用いた組織培養による植物体再生に関する研究
  塚田健太郎、山岸祐介、鍋嶋絵里、保坂路人、岡田健汰、Md Hasnat Rahman、半　智史、田端雅進、船田　良
  木材学会誌
  JAPAN WOOD RES SOC
  Resin and sap of lacquer trees (Toxicodendron vernicifluum) are called ”Urushi”. Urushi has been used for paints or adhesives since the Jomon period in Japan. Since it is prospective that demand for domestic Urushi for preservation of cultural property will increase, the mass production of seedlings of T. vernicifluum of high quality is important. We initially cultured immature zygotic embryos from immature fruits on Woody Plant Medium (WPM) containing 2,4-dichlorophenoxyacetic acid (2,4-D), alpha-naphthaleneacetic acid (NAA) or indole-3-acetic acid (IAA) as an auxin supplemented with polyvinylpyrrolidone (PVP). Somatic embryos were induced directly from immature zygotic embryos and secondary somatic embryos were induced from somatic embryos. Some secondary somatic embryos formed roots and hypocotyls. Finally, we transferred the secondary somatic embryo-derived plantlets to soil in pots and the plantlets continued to grow. Thus, we developed a tissue culture system for plant regeneration for the propagation of T. vernicifluum.
  2019年07月, 研究論文（学術雑誌）, 共同, 65, 3, 0021-4795, DOI(公開)(r-map), 125, 130
• Changes in cambial activity are related to precipitation patterns in four tropical hardwood species grown in Indonesia
  Rahman, Md Hasnat; Nugroho, Widyanto Dwi; Nakaba, Satoshi; Kitin, Peter; Kudo, Kayo; Yamagishi, Yusuke; Begum, Shahanara; Marsoem, Sri Nugroho; Funada, Ryo
  AMERICAN JOURNAL OF BOTANY
  WILEY
  Premise Cambial activity in some tropical trees varies intra-annually, with the formation of xylem rings. Identification of the climatic factors that regulate cambial activity is important for understanding the growth of such species. We analyzed the relationship between climatic factors and cambial activity in four tropical hardwoods, Acacia mangium, Tectona grandis, Eucalyptus urophylla, and Neolamarckia cadamba in Yogyakarta, Java Island, Indonesia, which has a rainy season (November-June) and a dry season (July-October). Methods Small blocks containing phloem, cambium, and xylem were collected from main stems in January 2014, October 2015 and October 2016, and examined with light microscopy for cambial cell division, fusiform cambial cells, and expanding xylem cells as evidence of cambial activity. Results During the rainy season, when precipitation was high, cambium was active. By contrast, during the dry season in 2015, when there was no precipitation, cambium was dormant. However, in October 2016, during the so-called dry season, cambium was active, cell division was conspicuous, and a new xylem ring formation was initiated. The difference in cambial activity appeared to be related to an unusual pattern of precipitation during the typically dry months, from July to October, in 2016. Conclusions Our results indicate that low or absent precipitation for 3 to 4 months induces cessation of cambial activity and temporal periodicity of wood formation in the four species studied. By contrast, in the event of continuing precipitation, cambial activity in the same trees may continue throughout the year. The frequency pattern of precipitation appears to be an important determinant of wood formation in tropical trees.
  2019年06月, 研究論文（学術雑誌）, 共同, 106, 6, 0002-9122, DOI(公開)(r-map), 760, 771
• The relationship between individual root anatomy and fine root system development in blueberry seedlings: dominance of diarch roots in initial root systems
  Takashi Baba, Satoshi Nakaba, Satoshi Noma, Ryo Funada, Takuya Ban
  Plant Root
  2019年05月, 研究論文（学術雑誌）, 共同, 13, DOI(公開)(r-map), 1, 8
• Mesophyll conductance to CO2 in leaves of Siebold's beech (Fagus crenata) seedlings under elevated ozone
  Watanabe, Makoto; Kamimaki, Yu; Mori, Marino; Okabe, Shigeaki; Arakawa, Izumi; Kinose, Yoshiyuki; Nakaba, Satoshi; Izuta, Takeshi
  JOURNAL OF PLANT RESEARCH
  SPRINGER JAPAN KK
  Ozone is an air pollutant that negatively affects photosynthesis in woody plants. Previous studies suggested that ozone-induced reduction in photosynthetic rates is mainly attributable to a decrease of maximum carboxylation rate (V-cmax) and/or maximum electron transport rate (J(max)) estimated from response of net photosynthetic rate (A) to intercellular CO2 concentration (C-i) (A/C-i curve) assuming that mesophyll conductance for CO2 diffusion (g(m)) is infinite. Although it is known that C-i-based V-cmax and J(max) are potentially influenced by g(m), its contribution to ozone responses in C-i-based V-cmax and J(max) is still unclear. In the present study, therefore, we analysed photosynthetic processes including g(m) in leaves of Siebold's beech (Fagus crenata) seedlings grown under three levels of ozone (charcoal-filtered air or ozone at 1.0- or 1.5-times ambient concentration) for two growing seasons in 2016-2017. Leaf gas exchange and chlorophyll fluorescence were simultaneously measured in July and September of the second growing season. We determined the A, stomatal conductance to water vapor and g(m,) and analysed A/C-i curve and A/C-c curve (C-c: chloroplast CO2 concentration). We also determined the Rubisco and chlorophyll contents in leaves. In September, ozone significantly decreased C-i-based V-cmax. At the same time, ozone decreased g(m), whereas there was no significant effect of ozone on C-c-based V-cmax or the contents of Rubisco and chlorophyll in leaves. These results suggest that ozone-induced reduction in C-i-based V-cmax is a result of the decrease in g(m) rather than in carboxylation capacity. The decrease in g(m) by elevated ozone was offset by an increase in C-i, and C-c did not differ depending on ozone treatment. Since C-c-based V-cmax was also similar, A was not changed by elevated ozone. We conclude that g(m) is an important factor for reduction in C-i-based V-cmax of Siebold's beech under elevated ozone.
  2018年11月, 研究論文（学術雑誌）, 共同, 131, 6, 0918-9440, DOI(公開)(r-map), 907, 914
• Stem gravitropism and tension wood formation in Acacia mangium seedlings inclined at various angles
  Nugroho, Widyanto Dwi; Nakaba, Satoshi; Yamagishi, Yusuke; Begum, Shahanara; Rahman, Md Hasnat; Kudo, Kayo; Marsoem, Sri Nugroho; Funada, Ryo
  ANNALS OF BOTANY
  OXFORD UNIV PRESS
  Background and Aims In response to a gravitational stimulus, angiosperm trees generally form tension wood on the upper sides of leaning stems in order to reorientate the stems in the vertical direction. It is unclear whether the angle of inclination from the vertical affects tension wood formation. This study was designed to investigate negative gravitropism, tension wood formation and growth eccentricity in Acacia mangium seedlings inclined at different angles. Methods Uniform seedlings of A. mangium were artificially inclined at 30 degrees, 45 degrees. 60 degrees and 90 degrees from the vertical and harvested, with non-inclined controls, 3 months later. We analysed the effects of the angle of inclination on the stem recovery angle. the anatomical features of tension wood and radial growth. Key Results Smaller inclination angles were associated with earlier stem recovery while stems subjected to greater inclination returned to the vertical direction after a longer delay. However, in terms of the speed of negative gravitopism towards the vertical, stems subjected to greater inclination moved more rapidly toward the vertical. There was no significant difference in terms of growth eccentricity among seedlings inclined at different angles. The 30 degrees-inclined seedlings formed the narrowest region of tension wood but there were no significant differences among seedlings inclined at 45 degrees. 60 degrees and 90 degrees. The 90 degrees-inclined seedlings formed thicker gelatinous layers than those in 30 degrees-, 45 degrees- and 60 degrees-inclined seedlings. Conclusion Our results suggest that the angle of inclination of the stem influences negative gravitropism, the width of the tension wood region and the thickness of gelatinous layers. Larger amounts of gelatinous fibres and thicker gelatinous layers might generate the higher tensile stress required for the higher speed of stem-recovery movement in A. mangium seedlings.
  2018年07月01日, 研究論文（学術雑誌）, 共同, 122, 1, 0305-7364, DOI(公開)(r-map), 87, 94
• Formation of new networks of earlywood vessels in seedlings of the deciduous ring-porous hardwood Quercus serrata in springtime
  Kudo, Kayo; Utsumi, Yasuhiro; Kuroda, Katsushi; Yamagishi, Yusuke; Nabeshima, Eri; Nakaba, Satoshi; Yasue, Koh; Takata, Katsuhiko; Funada, Ryo
  TREES-STRUCTURE AND FUNCTION
  SPRINGER HEIDELBERG
  In deciduous ring-porous hardwoods, the timing of the onset of conduction of water via the networks of the current year's earlywood vessels is very important for the growth of buds and shoots because the main pathways for conduction of water are the networks of the current year's earlywood vessels. The purpose of this study was to visualize the formation of the networks of first earlywood vessels in the current year's xylem of seedlings of the deciduous ring-porous hardwood Quercus serrata. We monitored the distribution of water in the current and the previous year's secondary xylem at the cellular level in upper, middle and lower regions of stems during the formation of earlywood vessels by cryo-scanning electron microscopy after freeze-etching. We also examined how changes in water distribution were correlated with leaf phenology. The contents of the first vessel elements in the upper region of the stem changed from cytoplasm-rich to water earlier than those in middle and lower regions of the stem when buds were increasing in size. At bud break, vessel elements were filled with water throughout the entire stem. When the cambium was dormant and during formation of earlywood vessels, the previous year's latewood vessels were filled with water. Our results showed that complete differentiation of vessel elements occurred earlier in upper regions of stems than in middle and lower regions. Moreover, the functional networks of the previous year's latewood vessels appeared to be involved in supplying water to new networks of earlywood vessels in the current year's xylem.
  2018年06月, 研究論文（学術雑誌）, 共同, 32, 3, 0931-1890, DOI(公開)(r-map), 725, 734
• Changes in the morphology and functions of vacuoles during the death of ray parenchyma cells in Cryptomeria japonica
  Arakawa, Izumi; Funada, Ryo; Nakaba, Satoshi
  JOURNAL OF WOOD SCIENCE
  SPRINGER JAPAN KK
  Changes in the morphology and functions of vacuoles provide useful information about the mechanism of cell death. In the present study, we monitored the morphology and contents of vacuoles during the death of ray parenchyma cells in the conifer Cryptomeria japonica. In differentiating xylem, ray parenchyma cells had large central vacuoles. In sapwood, vacuoles in ray parenchyma cells contained proteins, an indication that one of the main functions of these vacuoles might be protein storage. A dramatic decrease in the protein content of some vacuoles was detected in the intermediate wood before the initiation of vacuole rupture. Although vacuole rupture was detected from the intermediate wood to the outermost heartwood, some vacuoles were obviously enlarged in the inner intermediate wood. Condensed nuclei were first observed after the rupture of these large vacuoles in ray parenchyma cells. It seems plausible that the autolysis of the contents of ray parenchyma cells might be caused by the rupture of the enlarged vacuoles in the inner intermediate wood.
  2018年06月, 研究論文（学術雑誌）, 共同, 64, 3, 1435-0211, DOI(公開)(r-map), 177, 185
• Heterorhizy and fine root architecture of rabbiteye blueberry (Vaccinium virgatum) softwood-cuttings
  Baba, Takashi; Nakaba, Satoshi; Noma, Satoshi; Funada, Ryo; Ban, Takuya
  JOURNAL OF PLANT RESEARCH
  SPRINGER JAPAN KK
  All fine root systems consist of individual fine roots. Individual roots have morphological, anatomical, and functional heterogeneity (heterorhizy). Heterorhizy plays crucial roles in plant ecosystems. However, in many species, the heterorhizy and fine root system architecture based on individual root units are unclear. This study investigated heterorhizy along the root system architecture of Vaccinium virgatum Ait (rabbiteye blueberry) softwood-cuttings (propagated from annual shoots in growing season) using protoxylem groups (PGs), a classification according to the number of protoxylem poles, as an indicator of individual root traits. Individual roots of rabbiteye blueberry varied from monarch to heptarch. The frequency of roots with larger number of PGs decreased but those with smaller number of PGs increased from adventitious roots toward lateral roots with different branching levels. This architecture were stable among cultivars, collecting position of the cuttings, or indole acetic acids treatment. Individual root sizes and secondary growth were positively correlated with the PGs. These results indicate that branching itself strongly and broadly controls individual root traits. The individual roots were classified into two types: monarch and diarch roots with small size and lacking secondary growth (thought to be hair roots in core Ericaceae) and triarch or more PG roots with large size and showing secondary growth. These heterogeneous individual roots responded differently to the experimental factors. In particular, elongation of the large roots significantly contributed to increased total root length. These results mean that heterorhizic plasticity is a determinant of root system development and heterorhizic variation exists even under practical cutting condition. In conclusion, we demonstrated heterorhizy of rabbieye blueberry cuttings based on the strong relationships of PG, individual root morphology and growth potential, and root system architecture. This study also supports strong connection between root morphology and functional roles intermediated by the PG.
  2018年03月, 研究論文（学術雑誌）, 共同, 131, 2, 0918-9440, DOI(公開)(r-map), 271, 284
• Climate change and the regulation of wood formation in trees by temperature
  Begum, Shahanara; Kudo, Kayo; Rahman, Md Hasnat; Nakaba, Satoshi; Yamagishi, Yusuke; Nabeshima, Eri; Nugroho, Widyanto Dwi; Oribe, Yuichiro; Kitin, Peter; Jin, Hyun-O; Funada, Ryo
  TREES-STRUCTURE AND FUNCTION
  SPRINGER HEIDELBERG
  A better understanding of the influence of environmental conditions on wood formation should help to improve the radial growth of trees and to prepare for climate change. The cambial activity of trees is associated with seasonal cycles of activity and dormancy in temperate zones. The timing of cambial reactivation in early spring and dormancy in autumn plays an important role in determination of the cambial growth and the environmental adaptivity of temperate trees. This review focuses on the temperature regulation of the timing of cambial reactivation and xylem differentiation and highlights recent advances of bud growth in relation to cambial activity of temperate trees. In addition, we discuss relationships between the timing of cambial reactivation, start of xylem differentiation and changes in levels of storage materials to identify the source of the energy required for cell division and differentiation. We also present a summary of current understanding of the effects of rapid increases and decreases in temperature on cambial activity, by localized heating and cooling, respectively. Increases in temperature from late winter to early spring influence the physiological processes that are involved in the initiation of cambial reactivation and xylem differentiation both in localized heated stems and under natural conditions. Localized cooling has a direct effect on cell expansion, the thickening of walls of differentiating tracheids, and the rate of division of cambial cells. A rapid decrease in temperature of the stem might be the critical factor in the control of latewood formation and the cessation of cambial activity. Therefore, temperature is the main driver of cambial activity in temperate trees and trees are able to feel changes in temperature through the stem. The climate change might affect wood formation in trees.
  2018年02月, 研究論文（学術雑誌）, 共同, 32, 1, 0931-1890, DOI(公開)(r-map), 3, 15
• Effects of auxin-transport-inhibitor and defoliation on wood formation in locally-heated Abies homolepis
  Rahman, Md Hasnat; Kudo, Kayo; Begum, Shahanara; Yamagishi, Yusuke; Muraishi, Takahiro; Nakaba, Satoshi; Oribe, Yuichiro; Lee, Chanhui; Jin, Hyun-O; Funada, Ryo
  IAWA JOURNAL
  BRILL ACADEMIC PUBLISHERS
  To understand the precise process of wood formation, it is necessary to identify the factors that regulate cambial activity and development of cambial derivatives. Here, we investigated the combined effects of localized-heating and auxin on cambial reactivation and the formation of earlywood tracheids in seedlings of the evergreen conifer Abies homolepis in winter. Three treatments were applied, namely heating (artificial increase in temperature 20-22 degrees C), heating-plus-auxin transport inhibitor N-(1-naphthyl) phthalamic acid (NPA) and heating-plus-defoliation (removal of needles and buds), with an approximate control, for investigations of cambial activity by light microscopy. After one week of heating, cambial reactivation occurred in the heating, heating-plus-NPA and heating-plus-defoliation treatments. In untreated controls, cambial reactivation occurred later than in heated stems. Earlywood tracheids were formed after three and six weeks of heating in the heating and heating-plus-NPA treatments, respectively. No tracheids were formed after eight weeks of heating in heated-defoliated seedlings. Numbers of new tracheids were reduced in heated stems by NPA. Our results suggest that an increase in the temperature of the stem is one of the most important limiting factors in cambial reactivation, which is independent of needles and buds and of the polar transport of auxin from apical sources. However, after cambial reactivation, initiation and continuous formation of earlywood tracheids require basipetally transported auxin and other endogenous factors originating in mature needles and buds.
  2018年, 研究論文（学術雑誌）, 共同, 39, 4, 0928-1541, DOI(公開)(r-map), 353, 371
• Effects of submicron ammonium sulfate particles on the growth and yield of komatsuna (Brassica rapa L. var. perviridis)
  Motai, Akira; Nakaba, Satoshi; Lenggoro, I. Wuled; Watanabe, Makoto; Wada, Yoshiharu; Izuta, Takeshi
  ATMOSPHERIC ENVIRONMENT
  PERGAMON-ELSEVIER SCIENCE LTD
  The aim of this study was to determine the effects of submicron ammonium sulfate (AS) particles on komatsuna (Brassica rapa L. cv. Hakkei) plants. First, we optimized a leaf-washing method to measure the amount of AS particles deposited on the leaf surface of the plants. Then, we used this method to determine the retention time of particles deposited on the leaf surface of the plants. We also investigated the effects of AS particles on the growth and yield of the plants. Almost all the AS particles deposited on the leaf surface were removed within 1 min washing time with ultrapure water, and ion leaching from the leaf was relatively slow but continuous during the leaf-washing procedure. On the basis of these results, we determined that 1 min was a suitable washing time to remove most of the AS particles while minimizing the influence of ion leaching from the leaf. The amount of particulate SO42- deposited on the leaf surface decreased over time, probably because AS particles deposited on the leaf surface deliquesced, allowing ions such as SO42- in the deliquescence solution to be absorbed into the leaf. The plants were grown and exposed to AS particles for 16 days in naturally lit phytotrons. The daily mean increase in the concentration of SO42- in PM2.5 by the exposure to AS particles was 22.5 mu g m(-3) in the phytotrons. The growth and yield of the plants were significantly reduced by the exposure to AS particles. The exposure to AS particles did not affect the leaf concentrations of nitrogen and chlorophyll, but significantly reduced stomatal conductance. Therefore, stomatal closure is one of the reasons for the AS particle-induced reductions in the growth and yield of komatsuna plants. (C) 2017 Elsevier Ltd. All rights reserved.
  2017年11月, 研究論文（学術雑誌）, 共同, 169, 1352-2310, DOI(公開)(r-map), 278, 286
• Partial desiccation enhances induction of secondary xylem-like tracheary elements from calli of hybrid poplar (Populus sieboldii x P-grandidentata)
  Yamagishi, Yusuke; Yoshimoto, Joto; Ide, Suzuka; Nakaba, Satoshi; Nabeshima, Eri; Watanabe, Ugai; Funada, Ryo
  TREES-STRUCTURE AND FUNCTION
  SPRINGER HEIDELBERG
  Cells of hybrid poplar (Populus sieboldii x P. grandidentata) in culture can be induced to differentiate into secondary xylem-like tracheary elements that form the highly developed bordered pits and broad regions of cell walls in contrast to helical or reticulate wall thickenings in primary xylem elements. We attempted to increase the rate of differentiation of tracheary elements from calli using a combination of hormonal stimulation and partial desiccation. Calli that had been exposed to desiccation in air in a clean hood for 90 min before transfer to the induction medium differentiated into tracheary elements at higher rates than calli without air desiccation. The partial desiccation treatment had no effects on the features of the induced tracheary elements and the frequencies with which they appeared. Our results show that partial desiccation can increase, approximately threefold the rate of differentiation of secondary xylem-like tracheary elements from calli of hybrid poplar. This improvement in the rate of differentiation tracheary elements in vitro should facilitate detailed future analysis of the differentiation of secondary xylem.
  2017年06月, 研究論文（学術雑誌）, 共同, 31, 3, 0931-1890, DOI(公開)(r-map), 1083, 1089
• Cell wall formation of wood: The roles of cortical microtubles
  Funada, Ryo; Yamagishi, Yusuke; Yoshimoto, Joto; Begum, Shahanara; Nugroho, Widyanto Dwi; Kudo, Kayo; Rahman, Md Hasnat; Watanabe, Ugai; Nakaba, Satoshi
  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
  AMER CHEMICAL SOC
  2017年04月, 研究論文（学術雑誌）, 共同, 253, 0065-7727
• Responses of ray parenchyma cells to wounding differ between earlywood and latewood in the sapwood of Cryptomeria japonica
  Nakaba, Satoshi; Morimoto, Hikaru; Arakawa, Izumi; Yamagishi, Yusuke; Nakada, Ryogo; Funada, Ryo
  TREES-STRUCTURE AND FUNCTION
  SPRINGER HEIDELBERG
  Changes in cellular contents of ray parenchyma cells during the formation of reaction zone differ between earlywood and latewood in the sapwood of Cryptomeria japonica. Changes over time in the cellular contents of xylem parenchyma cells provide important clues to the mechanism of the early events in the wound reaction of trees. In this study, we monitored the events that occur during the death of ray parenchyma cells after wounding. We examined nuclei, starch grains, and colored substances in ray parenchyma cells by light microscopy and the autofluorescence of cell walls of tracheids by confocal laser-scanning microscopy in Cryptomeria japonica after artificial wounding. In addition, we compared cytological changes in ray parenchyma cells in the longitudinal and radial directions. Finally, we analyzed the differences between earlywood and latewood in terms of the responses of ray parenchyma cells to wounding. Behind the wound, changes in cellular contents were visible first in latewood regions in the second annual ring behind the wound. The progression of changes in cellular contents of ray parenchyma cells stopped near the growth-ring boundary. These results indicate that the growth-ring boundary might prevent the spread of some factor(s) that induces cytological changes in ray parenchyma cells. Above the wound, most colored substances were localized in ray parenchyma cells that were located near wounds in latewood regions. Thus, even at an equal distance from the wound, the amount of secondary metabolites in ray parenchyma cells differed between earlywood and latewood. Our observations suggest that differences in the anatomical features of neighboring tracheids between earlywood and latewood might influence changes in cellular contents of ray parenchyma cells during reactions to wounding in Cryptomeria japonica.
  2017年02月, 研究論文（学術雑誌）, 共同, 31, 1, 0931-1890, DOI(公開)(r-map), 27, 39
• Relationship between the earlywood-to-latewood transition and changes in levels of stored starch around the cambium in locally heated stems of the evergreen conifer Chamaecyparis pisifera
  Md Hasnat Rahman, Shahanara Begum, Satoshi Nakaba, Yusuke Yamagishi, Kayo Kudo, Eri Nabeshima, Widyanto Dwi Nugroho, Yuichiro Oribe, Ryo Funada
  Trees – Structure and Function
  2016年10月, 研究論文（学術雑誌）, 共同, 30, 1619, 1631
• Response in tree growth and wood structure of Larix kaempferi, Kalopanax septemlobus and Betula platyphylla saplings to elevated CO2 concentration for 5 years exposure in a FACE system
  Yoko Watanabe, Keita Wakabayashi, Satoshi Kitaoka, Takami Satomura, Norikazu Eguchi, Makoto Watanabe, Satoshi Nakaba, Kentaro Takagi, Yuzou Sano, Ryo Funada, Takayoshi Koike
  Trees – Structure and Function
  2016年10月, 研究論文（学術雑誌）, 共同, 30, 1569, 1579
• Effect of epicuticular wax crystals on the localization of artificially deposited sub-micron carbon-based aerosols on needles of Cryptomeria japonica
  Satoshi Nakaba, Kenichi Yamane, Mie Fukahori, Widyanto Dwi Nugroho, Masahiro Yamaguchi, Katsushi Kuroda, Yuzou Sano, I. Wuled Lenggoro, Takeshi Izuta, Ryo Funada
  Journal of Plant Research
  2016年09月, 研究論文（学術雑誌）, 共同, 129, 873, 881
• Agatharesinol biosynthesis-related changes of ray parenchyma in sapwood sticks of Cryptomeria japonica during cell death
  Satoshi Nakaba, Hikaru Morimoto, Izumi Arakawa, Nobumasa Bito, Takanori Imai, Ryogo Nakada, Ryo Funada
  Planta
  2016年05月, 研究論文（学術雑誌）, 共同, 243, 1225, 1236
• ブルーベリー果実の重量, 形態および吸水特性が裂果感受性の品種間差に及ぼす影響
  馬場 隆士，Fraidoon Karimi，半 智史，船田 良，伴 琢也
  園芸学研究
  2016年05月, 研究論文（学術雑誌）, 共同, 15, 179, 187
• Cavitation of intercellular spaces is critical to establishment of hydraulic properties of compression wood of Chamaecyparis obtusa seedlings
  Satoshi Nakaba, Asami Hirai, Kayo Kudo, Yusuke Yamagishi, Kenichi Yamane, Katsushi Kuroda, Widyanto Dwi Nugroho, Peter Kitin, Ryo Funada
  Annals of Botany
  2016年03月, 研究論文（学術雑誌）, 共同, 117, 457, 463
• Localized cooling of stems induces latewood formation and cambial dormancy during seasons of active cambium in conifers
  Shahanara Begum, Kayo Kudo, Yugo Matsuoka, Satoshi Nakaba, Yusuke Yamagishi, Eri Nabeshima, Md. Hasnat Rahman, Widyanto Dwi Nugroho, Yuichiro Oribe, Hyun-O Jin, Ryo Funada
  Annals of Botany
  2016年03月, 研究論文（学術雑誌）, 共同, 117, 465, 477
• 吸水によるブルーベリー果実の裂果発生要因の解析と吸水経路の特定
  馬場 隆士，長谷川 莉代，半 智史，船田 良，伴 琢也
  園芸学研究
  2015年09月, 研究論文（学術雑誌）, 共同, 14, 231, 239
• Continuous expression of genes for xylem cysteine peptidases in long-lived ray parenchyma cells in Populus.
  Satoshi Nakaba, Naoki Takata, Maokoto Yoshida, Ryo Funada
  Plant Biotechnology
  2015年04月, 研究論文（学術雑誌）, 共同, 32, 21, 29
• Structure and development of male pheromone gland of longicorn beetles and its phylogenetic relationships within the tribe Clytini.
  Keita Hoshino, Satoshi Nakaba, Hiroki Inoue, Kikuo Iwabuchi
  Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
  2015年04月, 研究論文（学術雑誌）, 共同, 324, 68, 76
• In vitro induction of the formation of tracheary elements from suspension-cultured cells of the conifer Cryptomeria japonica.
  Yusuke Yamagishi, Hiromu Uchiyama, Takenao Sato, Kei Kitamura, Joto Yoshimoto, Satoshi Nakaba, Ugai Watanabe, Ryo Funada
  Trees – Structure and Function
  2015年01月, 研究論文（学術雑誌）, 共同, 29, 1283, 1289
• Increased lodging resistance in long-culm, low-lignin gh2 rice for improved feed and bioenergy production.
  Taiichiro Ookawa, Kazuya Inoue, Makoto Matsuoka, Takeshi Ebitani, Takeshi Takarada, Toshio Yamamoto, Tadamasa Ueda, Tadashi Yokoyama, Chisato Sugiyama, Satoshi Nakaba, Ryo Funada, Hiroshi Kato, Motoki Kanekatsu, Koki Toyoda, Takashi Motobayashi, Mehran Vazirzanjani, Seisyu Tojo, Tadashi Hirasawa
  Scientific Reports
  2014年09月, 研究論文（学術雑誌）, 共同, 4, 6567
• The effects of localized heating and disbudding on cambial reactivation and formation of earlywood vessels in seedlings of the deciduous ring-porous hardwood, Quercus serrata.
  Kayo Kudo, Eri Nabeshima, Shahanara Begum, Yusuke Yamagishi, Satoshi Nakaba, Yuichiro Oribe, Ko Yasue, Ryo Funada
  Annals of Botany
  2014年05月, 研究論文（学術雑誌）, 共同, 113, 1021, 1027
• Effects of long-term exposure to ammonium sulfate particles on growth and gas exchange rates of Fagus crenata, Castanopsis sieboldii, Larix kaempferi and Cryptomeria japonica seedlings.
  Masahiro Yamaguchi, Yoko Otani, Peiran Li, Hiroshi Nagao, I. Wuled Lenggoro, Atsushi Ishida, Kenichi Yazaki, Kyotaro Noguchi, Satoshi Nakaba, Kenichi Yamane, Katsushi Kuroda, Yuzou Sano, Ryo Funada, Takeshi Izuta
  Atmospheric Environment
  2014年01月, 研究論文（学術雑誌）, 共同, 97, 493, 500
• エアロゾルの樹木への吸収・吸着機構の解明
  山根健一、半　智史、山口真弘、山岸祐介、Widyanto Dwi Nugroho、黒田克史、佐野雄三、Wuled Lenggoro、船田　良
  エアロゾル研究
  2013年10月, 共同, 特別号
• 森林樹木に対するブラックカーボン粒子の影響に関する実験的研究
  伊豆田猛、山口真弘、山根健一、半　智史、石田　厚、矢崎健一、野口享太郎、Wuled Lenggoro
  エアロゾル研究
  2013年10月, 共同, 特別号
• Gibberellin mediates the development of gelatinous fibers in the tension wood of inclined Acacia mangium seedlings
  Widyanto Dwi Nugroho, Satoshi Nakaba, Yusuke Yamagishi, Shahanara Begum, Sri Nugroho Marsoem, Jae-Heung Ko, Hyun-O Jin, Ryo Funada
  Annals of Botany
  2013年07月, 研究論文（学術雑誌）, 共同, 112, 1321, 1329
• Disappearance of microtubules, nuclei and starch during cell death of ray parenchyma in Abies sachalinensis
  Satoshi Nakaba, Yuzo Sano, Ryo Funada
  IAWA Journal
  2013年02月, 研究論文（学術雑誌）, 共同, 34, 135, 146
• In vitro induction of secondary xylem-like tracheary elements in calli of hybrid poplar (Populus sieboldii x P. grandidentata)
  Yusuke Yamagishi, Joto Yoshimoto, Hiromu Uchiyama, Eri Nabeshima, Satoshi Nakaba, Ugai Watanabe, Ryo Funada
  Planta
  2013年01月, 研究論文（学術雑誌）, 共同, 237, 1179, 1185
• Regulation of cambial activity in relation to environmental conditions: understanding the role of temperature in wood formation of trees
  Begum S, Nakaba S, Yamagishi Y, Oribe Y, Funada R
  Physiologia Plantarum
  2013年01月, 共同, 147, 46, 54
• Visualization of the mycelia of wood rotting fungi by fluorescence in situ hybridization using a peptide nucleic acid probe
  Nakada Y, Nakaba S, Matsunaga H, Funada R, Yoshida M
  Bioscience, Biotechnology and Biochemistry
  2012年12月, 研究論文（学術雑誌）, 共同, 77, 405, 408
• Visualization of artificially deposited submicron-sized aerosol particles on the surfaces of leaves and needles in trees
  Yamane K, Nakaba S, Yamaguchi M, Kuroda K, Sano Y, Lenggoro IW, Izuta T, Funada R
  Asian Journal of Atmospheric Environment
  2012年12月, 研究論文（学術雑誌）, 共同, 6, 275, 280
• Effects of long-term exposure of black carbon particles on growth and gas exchange rates of Fagus crenata, Castanopsis sieboldii, Larix kaempferi and Cryptomeria japonica seedlings
  Yamaguchi M, Otani Y, Takeda K, Lenggoro W, Ishida A, Yazaki K, Noguchi K, Sase H, Murao N, Nakaba S, Yamane K, Kuroda K, Sano Y, Funada R, Izuta T
  Asian Journal of Atmospheric Environment
  2012年12月, 研究論文（学術雑誌）, 共同, 6, 259, 267
• Tracheary elements that resemble secondary xylem in calli derived from the conifers, Torreya nucifera and Cryptomeria japonica
  Yamagishi Y, Sato T, Uchiyama H, Yoshimoto J, Nakagawa R, Nakaba S, Kubo T, Funada R
  Journal of Wood Science
  2012年12月, 研究論文（学術雑誌）, 共同, 58, 557, 562
• A rapid decrease in temperature induces latewood formation in artificially reactivated cambium of conifer stems
  Begum S, Nakaba S, Yamagishi Y, Yamane K, Islam AM, Oribe Y, Ko JH, Jin HO, Funada R
  Annals of Botany
  2012年08月, 研究論文（学術雑誌）, 共同, 110, 875, 885
• Gibberellin is required for the formation of tension wood and stem gravitropism in Acacia mangium seedlings
  Nugroho WD, Yamagishi Y, Nakaba S, Fukuhara S, Begum S, Marsoem SN, Ko JH, Jin HO, Funada R
  Annals of Botany
  2012年08月, 研究論文（学術雑誌）, 共同, 110, 887, 895
• DWARF50 (D50), a rice (Oryza sativa L.) gene encoding inositol polyphosphate 5-phosphatase, is required for proper development of intercalary meristem
  Sato-Izawa K, Nakaba S, Tamura K, Yamagishi Y, Nakano Y, Nishikubo N, Kawai S, Kajita S, Ashikari M, Funada R, Katayama Y, Kitano H
  Plant Cell and Environment
  2012年06月, 研究論文（学術雑誌）, 共同, 35, 2031, 2044
• Radial variations in the anatomical characteristics and density of the wood of Acacia mangium of five different provenances in Indonesia
  Nugroho WD, Marsoem SN, Yasue K, Fujiwara T, Nakajima T, Hayakawa M, Nakaba S, Yamagishi Y, Jin HO, Kubo T, Funada R
  Journal of Wood Science
  2012年06月, 研究論文（学術雑誌）, 共同, 58, 69, 76
• Differences in the timing of cell death, differentiation and function among three different types of ray parenchyma cells in the hardwood Populus sieboldii x P. grandidentata
  Satoshi Nakaba, Shahanara Begum, Yusuke Yamagishi, Hyun-O Jin, Takafumi Kubo, Ryo Funada
  Trees - Structure and Function
  2012年06月, 研究論文（学術雑誌）, 共同, 26, 743, 750
• Temporally and spatially controlled death of parenchyma cells is involved in heartwood formation in pith regions of branches of Robinia pseudoacacia var. inermis
  Satoshi Nakaba, Yusuke Yamagishi, Yuzou Sano, Ryo Funada
  Journal of Wood Science
  2012年02月, 研究論文（学術雑誌）, 共同, 58, 69, 76
• Cold stability of microtubules in wood-forming tissues of conifers during seasons of active and dormant cambium
  Shahanara Begum, Masaki Shibagaki, Osamu Furusawa, Satoshi Nakaba, Yusuke Yamagishi, Joto Yoshimoto, Hyun-O Jin, Yuzou Sano, Ryo Funada
  Planta
  2012年01月, 研究論文（学術雑誌）, 共同, 235, 165, 179
• Nuclear DNA fragmentation during cell death of short-lived ray tracheids in the conifer Pinus densiflora
  Satoshi Nakaba, Takafumi Kubo, Ryo Funada
  Journal of Plant Research
  2011年05月, 研究論文（学術雑誌）, 共同, 124, 379, 384
• Changes in the localization and levels of starch and lipids in cambium and phloem during cambial reactivation by artificial heating of main stems of Cryptomeria japonica trees
  Shahanara Begum, Satoshi Nakaba, Yuichiro Oribe, Takafumi Kubo, Ryo Funada
  Annals of Botany
  2010年12月, 研究論文（学術雑誌）, 共同, 106, 885, 895
• Overexpression of a fungal laccase gene induces non-dehiscent anthers and morphological changes in flowers of transgenic tobacco
  Zannatul Nasrin, Misato Yoshikawa, Yuki Nakamura, Shahanara Begum, Satoshi Nakaba, Mikiko Uesugi, Yuriko Osakabe, Tomonori Sonoki, Kanna Sato, Ryo Funada, Yosuke Iimura, Yoshihiro Katayama, Shinya Kajita
  Journal of Wood Science
  2010年12月, 研究論文（学術雑誌）, 共同, 56, 460, 469
• The carbohydrate-binding module (CBM)-like sequence is a crucial for rice CWA1/BC1 function in proper assembly of secondary cell wall materials
  Kanna Sato, Sachiko Ito, Takeo Fujii, Ryu Suzuki, Sachi Takenouchi, Satoshi Nakaba, Ryo Funada, Yuzou Sano, Shinya Kajita, Hidemi Kitano, Yoshihiro Katayama
  Plant Signaling and Behavior
  2010年11月, 研究論文（学術雑誌）, 共同, 5, 1433, 1436
• Isolation of a novel cell wall architecture mutant of rice with defective Arabidopsis COBL4 ortholog BC1 required for regulated deposition of secondary cell wall components
  Kanna Sato, Ryu Suzuki, Nobuyuki Nishikubo, Sachi Takenouchi, Sachiko Ito, Yoshimi Nakano, Satoshi Nakaba, Yuzou Sano, Ryo Funada, Shinya Kajita, Hidemi Kitano, Yoshihiro Katayama
  Planta
  2010年05月, 研究論文（学術雑誌）, 共同, 232, 257, 270
• 変性剤濃度勾配ゲル電気泳動法を利用した腐朽木材中に存在する真菌類の菌叢解析
  中田 裕治，久住 朝子，半 智史，片山 葉子，船田 良，福田 清春，吉田 誠
  木材保存
  2010年02月, 研究論文（学術雑誌）, 共同, 36, 100, 110
• Cambial sensitivity to rising temperatures by natural condition and artificial heating from late winter to early spring in the evergreen conifer Cryptomeria japonica
  Shahanara Begum, Satoshi Nakaba, Yuichiro Oribe, Takafumi Kubo, Ryo Funada
  Trees - Structure and Function
  2010年01月, 研究論文（学術雑誌）, 共同, 24, 43, 52
• 樹木の二次木部細胞におけるセルロースミクロフィブリルの配向制御
  船田　良、半　智史
  Cellulose Communications（セルロース学会誌）
  2009年07月, 共同, 16, 95, 101
• Temperature responses of cambial reactivation and xylem differentiation in hybrid poplar (Populus sieboldii x P. grandidentata) under natural conditions
  Shahanara Begum, Satoshi Nakaba, Vilma Bayramzadeh, Yuichiro Oribe, Takafumi Kubo, Ryo Funada
  Tree Physiology
  2008年12月, 研究論文（学術雑誌）, 共同, 28, 1813, 1819
• Morphological changes in the cytoskeleton, nuclei and vacuoles during the cell death of short-lived ray tracheids in the conifer Pinus densiflora
  Satoshi Nakaba, Joto Yoshimoto, Takafumi Kubo, Ryo Funada
  Journal of Wood Science
  2008年12月, 研究論文（学術雑誌）, 共同, 54, 509, 514
• Differences in patterns of cell death between ray parenchyma cells and ray tracheids in the conifers Pinus densiflora and Pinus rigida
  Satoshi Nakaba, Takafumi Kubo, Ryo Funada
  Trees - Structure and Function
  2008年08月, 研究論文（学術雑誌）, 共同, 22, 623, 630
• Gibberellin-induced formation of tension wood in angiosperm trees
  Ryo Funada, Tatsuhiko Miura, Yousuke Shimizu, Takanori Kinase, Satoshi Nakaba, Takafumi Kubo, Yuzou Sano
  Planta
  2008年06月, 研究論文（学術雑誌）, 共同, 227, 1409, 1414
• Induction of cambial reactivation by localized heating in a deciduous hardwood hybrid poplar (Populus sieboldii x P. grandidentata)
  Shahanara Begum, Satoshi Nakaba, Yuichiro Oribe, Takafumi Kubo, Ryo Funada
  Annals of Botany
  2007年09月, 研究論文（学術雑誌）, 共同, 100, 439, 447
• Influence of overexpression of a gibberellin 20-oxidase gene on the kinetics of xylem cell development in hybrid poplar (Populus tremura L. and P. tremuloides Michx.)
  Oliver Dünisch, Matthias Fladung, Satoshi Nakaba, Yoko Watanabe, Ryo Funada
  Holzforschung
  2006年09月, 研究論文（学術雑誌）, 共同, 60, 608, 617
• The positional distribution of cell death of ray parenchyma cells in a conifer, Abies sachalinensis
  Satoshi Nakaba, Yuzou Sano, Takafumi Kubo, Ryo Funada
  Plant Cell Reports
  2006年09月, 研究論文（学術雑誌）, 共同, 25, 1143, 1148

著書

• 木質の形成（第3版）
  海青社
  2024年03月
• 森林・林業実務必携　第2版
  東京農工大学農学部　森林・林業実務必携編集委員会 編
  朝倉書店
  2021年04月
• 樹皮の識別 IAWAによる光学顕微鏡的特徴リスト
  佐野雄三、吉永　新、半　智史
  海青社
  2021年03月
• 木材科学講座4 木材の化学
  川田俊成・伊藤和貴編
  海青社
  2021年03月
• 木本植物の生理生態
  小池 孝良・北尾 光俊・市栄 智明・渡辺 誠編
  共立出版
  2020年11月
• Xylogenesis in trees: from cambial cell division to cell death.「Secondary xylem biology」
  Funada, R., Yamagishi, Y., Begum, S., Kudo, K., Nabeshima, E., Nugroho,W.D., Oribe, Y., Nakaba, S. (Kim, Y.S., Funada, R., Singh, A. 編著)
  Elsevier
  2016年01月
• Three-dimensional imaging of cambium and secondary xylem cells by confocal laser scanning microscopy.「Plant Microtechniques and Protocols」
  Nakaba, S., Kitin, P., Yamagishi, Y., Begum, S., Kudo, K., Nugroho,W.D., Funada, R. (Yeung, E.C.T., Stasolla, C., Summer, M.J., Huang, B.Q. 編著)
  Springer
  2015年10月

研究発表、招待講演等

• カキノキ木部の黒色化過程における自家蛍光の変化
  第74回日本木材学会大会　京都
  2024年03月, ポスター発表
• スギ師部放射組織の細胞間隙の発達過程
  第74回日本木材学会大会　京都
  2024年03月, ポスター発表
• ウコギ科広葉樹の細胞間道の構造と内容物に関する解剖学的および組織化学的研究
  第74回日本木材学会大会　京都
  2024年03月, ポスター発表
• Eucalyptus nitens における引張あて材の量と屈曲角度の関係
  第74回日本木材学会大会　京都
  2024年03月, ポスター発表
• ユーカリ3樹種の傾斜地における苗木の成長特性の樹種間比較
  第74回日本木材学会大会　京都
  2024年03月, ポスター発表
• 生育速度の異なるスギにおける放射柔細胞の生存率およびデンプン貯蔵量の放射方向での推移
  第74回日本木材学会大会　京都
  2024年03月, ポスター発表
• ヒメバラモミ成熟種子から不定胚形成細胞を経由した植物体再生
  第74回日本木材学会大会　京都
  2024年03月, ポスター発表
• 交雑ポプラ管状要素誘導系に与えるオーキシンの種類の影響
  第74回日本木材学会大会　京都
  2024年03月, ポスター発表
• 赤色/青色光下で育成したユーカリ苗木の光屈性及び木材組織観察
  第74回日本木材学会大会　京都
  2024年03月, 口頭発表（一般）
• 電界放出形走査電子顕微鏡（FE-SEM）によるスギ放射柔細胞の辺材中における細胞内容物の変化の解析
  第74回日本木材学会大会　京都
  2024年03月, 口頭発表（一般）
• ドロノキ放射柔細胞におけるプロテアーゼRD21の組織および細胞内局在の季節変動の免疫標識法による解析
  第74回日本木材学会大会　京都
  2024年03月, 口頭発表（一般）
• 植物組織培養によるヒノキ成熟胚から誘導したカルスからの不定芽形成
  第74回日本木材学会大会　京都
  2024年03月, 口頭発表（一般）
• The effects of localized cooling and precipitation on cambial activity of deciduous hardwood Quercus serrata in the summer and autumn seasons
  第74回日本木材学会大会　京都
  2024年03月, 口頭発表（一般）
• フェノール性成分がオニグルミの心材色発現に与える影響
  第73回日本木材学会大会　福岡
  2023年03月, ポスター発表
• ニセアカシア樹幹放射方向における細胞レベルの水分布の解析
  第73回日本木材学会大会　福岡
  2023年03月, ポスター発表
• 重力方向の変化に伴う交雑ポプラのデンプン鞘細胞におけるデンプン粒の挙動
  第73回日本木材学会大会　福岡
  2023年03月, ポスター発表
• ジャスモン酸メチルおよびエテホン処理による広葉樹の傷害細胞間道形成と師部の形態変化に関する解剖学的研究
  第73回日本木材学会大会　福岡
  2023年03月, ポスター発表
• 組織培養によるヒノキ植物体再生に関する研究
  第73回日本木材学会大会　福岡
  2023年03月, ポスター発表
• FE-SEMとBIB法による木本植物の組織構造のインタクト観察
  第73回日本木材学会大会　福岡
  2023年03月, 口頭発表（一般）
• 赤色光下で育成したユーカリ苗木の形態および木材組織観察
  第73回日本木材学会大会　福岡
  2023年03月, 口頭発表（一般）
• カラマツにおける樹幹木部肥大成長への光合成産物配分の季節変動
  第73回日本木材学会大会　福岡
  2023年03月, 口頭発表（一般）
• Cambial response on the stem of deciduous Ginkgo biloba to increasing ambient air temperatures from late winter to spring
  第73回日本木材学会大会　福岡
  2023年03月, 口頭発表（一般）
• カラマツとスギの樹幹における非構造性炭水化物量の放射方向および季節変動に関する研究
  第73回日本木材学会大会　福岡
  2023年03月, 口頭発表（一般）
• ジャスモン酸メチルおよびエテホン処理によるカキノキ木部の黒色化誘導に関する研究
  第73回日本木材学会大会　福岡
  2023年03月, 口頭発表（一般）
• 人為的に黒色化を誘導したカキノキ木部に含まれる抽出成分の分析
  第73回日本木材学会大会　福岡
  2023年03月, 口頭発表（一般）
• ドロノキ放射柔細胞におけるプロテアーゼRD21の組織内局在の季節変動
  第73回日本木材学会大会 福岡
  2023年03月, 口頭発表（一般）
• Cell death of long-lived xylem ray parenchyma cells during heartwood formation
  The First International Mini-symposium on Wood Science – Towards a Brilliant Future with Wood Science –
  2022年11月, 口頭発表（招待・特別）
• エゾマツの成熟種子胚を用いた組織培養による植物体再生に関する研究
  第72回日本木材学会大会　名古屋・岐阜
  2022年03月, ポスター発表
• ドロノキの培養細胞を用いた管状要素誘導に関する研究
  第72回日本木材学会大会　名古屋・岐阜
  2022年03月, ポスター発表
• ヒノキ成熟胚からの組織培養に関する研究
  第72回日本木材学会大会　名古屋・岐阜
  2022年03月, ポスター発表
• 赤外分光法によるカキノキの木部の黒色部における着色物質の成分分析
  第72回日本木材学会大会　名古屋・岐阜
  2022年03月, ポスター発表
• カキノキ木部の黒色化における着色物質の拡散経路の Resin cast replica 法による観察
  第72回日本木材学会大会　名古屋・岐阜
  2022年03月, ポスター発表
• カラマツ樹幹の放射方向における非構造性炭水化物含有量の季節変動
  第72回日本木材学会大会　名古屋・岐阜
  2022年03月, ポスター発表
• スギの樹皮での傷害周皮形成の期間と分布の季節変化
  第72回日本木材学会大会　名古屋・岐阜
  2022年03月, ポスター発表
• スギ内樹皮放射組織の細胞間隙における結晶と多糖類に関する解析
  第72回日本木材学会大会　名古屋・岐阜
  2022年03月, 口頭発表（一般）
• ニセアカシア心材成分の沈着過程に関する蛍光スペクトル解析
  第72回日本木材学会大会　名古屋・岐阜
  2022年03月, 口頭発表（一般）
• Temperature responses of cambial activity cessation and leaf color changes in Ginkgo biloba trees during the autumn and winter
  第72回日本木材学会大会　名古屋・岐阜
  2022年03月, 口頭発表（一般）
• オオヤマレンゲの未成熟種子由来の PEMs を経由した組織培養による植物体再生に関する研究
  第72回日本木材学会大会　名古屋・岐阜
  2022年03月, 口頭発表（一般）
• ESM(Embryogenic suspensor masses)を経由したトドマツの植物体再生
  第72回日本木材学会大会　名古屋・岐阜
  2022年03月, 口頭発表（一般）
• Effects of localized heating on the cambial reactivation and resin canal formation of Toxicodendron vernicifluum
  第72回日本木材学会大会　名古屋・岐阜
  2022年03月, 口頭発表（一般）
• スギの傷害コルク形成層形成過程における師部柔細胞の形態変化
  第71回日本木材学会大会　東京
  2021年03月, 口頭発表（一般）
• ロビネチン滴下実験を組み合わせたニセアカシアの心材成分沈着過程の解析
  第71回日本木材学会大会　東京
  2021年03月, ポスター発表
• スギ木部放射組織における物質移動経路の可視化手法の検討
  第71回日本木材学会大会　東京
  2021年03月, ポスター発表
• スギ内樹皮放射組織の細胞間隙における結晶と多糖類の分布
  第71回日本木材学会大会　東京
  2021年03月, ポスター発表
• 交雑ポプラ放射柔細胞の細胞死過程における内容物の変化
  第71回日本木材学会大会　東京
  2021年03月, ポスター発表
• カキノキ木部の黒色化における着色物質の蓄積および拡散過程
  第71回日本木材学会大会　東京
  2021年03月, ポスター発表
• カラマツ樹幹の放射方向におけるデンプン量の変動
  第71回日本木材学会大会　東京
  2021年03月, ポスター発表
• ニセアカシアの心材形成過程における放射柔細胞の細胞死および心材成分の沈着の観察
  第71回日本木材学会大会　東京
  2021年03月, ポスター発表
• エゾマツの成熟種子胚を用いた組織培養による植物体再生に関する研究
  第71回日本木材学会大会　東京
  2021年03月, ポスター発表
• オオヤマレンゲの未成熟種子を用いた組織培養による植物体再生および順化に関する 研究
  第71回日本木材学会大会　東京
  2021年03月, ポスター発表
• 交雑ポプラ管状要素誘導における誘導率と細胞形態に与える植物成長調節物質の影響
  第71回日本木材学会大会　東京
  2021年03月, ポスター発表
• トドマツ成熟種子からの不定胚形成細胞を経由した植物体再生
  第71回日本木材学会大会　東京
  2021年03月, ポスター発表
• ジャスモン酸メチル塗布処理によって誘導されるトドマツの傷害樹脂道形成に局所的加温処理が与える影響
  第71回日本木材学会大会　東京
  2021年03月, ポスター発表
• インドネシア産樹木 4 種の外樹皮の組織構造
  日本植物学会　第84回大会　名古屋
  2020年09月21日, ポスター発表
• 樹木の心材形成過程の解析への蛍光スペクトルイメージングの応用
  日本植物学会　第84回大会　名古屋
  2020年09月21日, ポスター発表
• 落葉広葉樹環孔材コナラの休眠期樹幹に対する局所的加温処理が木部形成に与える影響
  第70回日本木材学会大会　鳥取
  2020年03月18日, 口頭発表（一般）
• 自家蛍光を用いた蛍光スペクトルイメージングによる放射柔細胞の細胞内容物の可視化条件の検討
  第70回日本木材学会大会　鳥取
  2020年03月18日, 口頭発表（一般）
• 白い木材の細胞壁層観察による高配向性木質材料への展望
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• グルコマンナン存在下における木質細胞壁の人工合成
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• オオヤマレンゲの未成熟種子を用いた組織培養による不定胚形成および植物体再生に関する研究
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• 植物組織培養によるイラモミ成熟種子からの植物体再生
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• カキノキ木部の黒色部位における着色物質の移動経路および木部繊維細胞壁への沈着
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• 蛍光トレーサーを用いたスギ放射組織における物質移動経路の解析
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• ドリアン属におけるタイルセルの解剖学的特徴と形態学的特徴
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• ドロノキ放射柔細胞の細胞死過程における細胞内容物の変化
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• 交雑ポプラ培養細胞の管状要素誘導系における細胞壁構造の観察
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• Sanded surface instead of slides of difficult-to-section woods―epifluorescence reveals micro-features of Dalbergia―
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• スギ放射組織の細胞間隙に関する解剖学的研究
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• Effect of gibberellin inhibitor on the wood anatomical characteristics of Wisteria floribunda
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• ジャスモン酸メチル塗布処理がウラジロモミ苗木の木部形成に与える影響の季節変化
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• 自家蛍光を用いたニセアカシアの心材成分沈着過程の解析
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• インドネシアに生育する樹木4種における樹皮の組織構造
  第70回日本木材学会大会　鳥取
  2020年03月17日, ポスター発表
• 天然のアクチュエーター"松かさ"を構成するマトリックス成分の機能解析
  第70回日本木材学会大会　鳥取
  2020年03月16日, 口頭発表（一般）
• リグニンフリーな広葉樹材の創出とその特性評価
  第70回日本木材学会大会　鳥取
  2020年03月16日, 口頭発表（一般）
• Effects of inclination on tension wood formation in locally heated stem of Quercus serrata
  第70回日本木材学会大会　鳥取
  2020年03月16日, 口頭発表（一般）
• 交雑ポプラ培養細胞への GFP-TUA6およびLifeact-mCherryの導入による細胞骨格の可視化
  第70回日本木材学会大会　鳥取
  2020年03月16日, 口頭発表（一般）
• スギ樹幹に誘導した傷害周皮の形態および空間分布の解剖学的解析
  日本植物学会　第83回大会　仙台
  2019年09月17日, ポスター発表
• カキノキ木部の黒色部位における着色物質の局在解析
  日本植物学会　第83回大会　仙台
  2019年09月17日, ポスター発表
• 樹木の木部放射組織における物質移動の蛍光トレーサーを用いた解析
  日本植物学会　第83回大会　仙台
  2019年09月15日, 口頭発表（一般）
• Effects of heating and inclination on tension wood formation in ring-porous hardwood Quercus serrata
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• スギの樹幹に対する局所的冷却処理が形成層分裂活動に与える影響
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• The effects of methyl jasmonate treatment on wood formation of Gyrinops versteegii seedlings
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• オーキシンの移動制御を目的とした剥皮およびNPA塗布処理によるヒノキの木部および形成層細胞の変化
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• ClearSee処理による樹木形成層の深部観察
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• 蛍光トレーサーを用いたケヤキの放射組織を経由する物質移動の観察
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• ニセアカシアの心材形成過程における心材成分と細胞内容物の蛍光スペクトルイメージングによる同時観察
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• カキノキ辺材部の黒色化に伴う木部柔細胞の内容物の変化
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• スギの傷害周皮形成過程における傷害からの方向の違いにより生じる解剖学的差異
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• 白いタケの創製およびそのキャラクタリゼーション
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• ブコウマメザクラの植物組織培養を用いた植物体の再生
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• 不定胚を経由したヤツガタケトウヒの植物体再生
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• トドマツの培養細胞を用いた管状要素分化誘導に関する研究
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• トドマツ成熟種子由来の不定胚形成細胞（ESM）経由の植物体再生系の確立
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• 様々な天然セルロースミクロフィブリルの長さ方向における高次構造評価
  第69回日本木材学会大会　函館
  2019年03月15日, ポスター発表
• オニグルミ樹幹に含有するフェノール成分の放射方向での変動と構成成分の化学特性
  第69回日本木材学会大会　函館
  2019年03月14日, 口頭発表（一般）
• 13CO2標識法を用いたスギ柔細胞におけるデンプン粒として貯蔵された光合成同化産物の分布の解析
  第69回日本木材学会大会　函館
  2019年03月14日, 口頭発表（一般）
• スギにおけるリチドームの形成過程と周皮の3次元的な形態
  第69回日本木材学会大会　函館
  2019年03月14日, 口頭発表（一般）
• 落葉広葉樹における形成層活動と木部形成の季節性
  第69回日本木材学会大会　函館
  2019年03月14日, 口頭発表（一般）
• 蛍光トレーサーを用いた木部放射組織における物質移動の可視化
  第69回日本木材学会大会　函館
  2019年03月14日, 口頭発表（一般）
• Cytological Changes in Ray Parenchyma Cells during Artificially Induced Cell Death in Cryptomeria japonica
  2018 SWST/JWRS International Convention, Nagoya Japan
  2018年11月, 口頭発表（一般）
• The role of microtubules on cell wall formation of wood
  2018 SWST/JWRS International Convention, Nagoya Japan
  2018年11月, 口頭発表（一般）
• Climatic Regulation of Cambial Activity on the Stem of Temperate and Tropical trees
  2018 SWST/JWRS International Convention, Nagoya Japan
  2018年11月, 口頭発表（一般）
• Comparative FT-IR analysis of wood blocks decayed by six species of brown-rot fungi
  2018 SWST/JWRS International Convention, Nagoya Japan
  2018年11月, ポスター発表
• Artificial lignified cell wall synthesis based on polysaccharide extracted from cultured cells
  2018 SWST/JWRS International Convention, Nagoya Japan
  2018年11月, ポスター発表
• Formation of networks of earlywood vessels in a deciduous ring-porous hardwood, Quercus serrata
  2018 SWST/JWRS International Convention, Nagoya Japan
  2018年11月, ポスター発表
• Development of a lignin-free hardwood block from Zelkova serrata and its structural evaluation
  2018 SWST/JWRS International Convention, Nagoya Japan
  2018年11月, ポスター発表
• 種々の褐色腐朽菌で腐朽させた木材の化学的特性
  第63回リグニン討論会
  2018年11月, ポスター発表
• 心材成分の自家蛍光を用いたスペクトルイメージング解析
  樹木抽出成分討論会　秋田
  2018年09月, 口頭発表（一般）
• カキノキ辺材部における黒色心材様組織の誘導
  樹木抽出成分討論会　秋田
  2018年09月, ポスター発表
• 異なる分岐群に属する褐色腐朽菌における木材腐朽様式の比較解析
  第34回日本木材保存協会年次大会
  2018年05月, ポスター発表
• 休眠期コナラ苗木樹幹に対する局所的加温とオーキシン塗布の複合処理が木部形成に与える影響
  第68回日本木材学会　京都
  2018年03月, 口頭発表（一般）
• トチノキ培養細胞を用いたせん孔をもつ管状要素の分化誘導
  第68回日本木材学会　京都
  2018年03月, 口頭発表（一般）
• Wood formation in tropical trees: understanding the changes in cambial activity in relation to the rainfall pattern in hardwood trees grown in Yogyakarta, Java Island, Indonesia
  第68回日本木材学会　京都　
  2018年03月, 口頭発表（一般）
• 心材形成過程の解析への自家蛍光を用いたスペクトルイメージングの応用
  第68回日本木材学会　京都
  2018年03月, 口頭発表（一般）
• 進化系統的に異なる分岐群に属する褐色腐朽菌間での木材分解様式の比較
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• リグニンフリーなケヤキ材の調製とその構造評価
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• ウルシの種子からのカルス誘導と有用物質の解析
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• ゲッケイジュのカルス培養およびオイゲノールの分析に関する研究
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• カキノキにおける黒色心材様組織の誘導
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• ウルシの種子を用いた組織培養による植物体再生に関する研究
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• トドマツ不定胚形成細胞（ESM）からの不定胚形成および植物体再生に関する研究
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• オニグルミ樹幹におけるフェノール含有量の放射方向での変動
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• オーキシンの移動の制御を目的とした剥皮およびNPA塗布処理による形成層細胞の変化
  第68回日本木材学会大会　京都　
  2018年03月, ポスター発表
• 枝打ちがヒノキの早晩材付近の仮道管形態に与える影響
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• ドリアン属のタイルセルの解剖学的特徴とその分化過程
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• 蛍光プローブによるポプラ培養細胞の管状要素誘導系におけるリグニン沈着の可視化
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• カラマツ放射柔細胞の分化過程とデンプンおよび脂質の貯蔵開始のタイミングとの関連性
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• イチョウの二次木部における水分通道の季節変化
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• スギにおける周皮の分布と形成過程
  第68回日本木材学会大会　京都　
  2018年03月, ポスター発表
• チークの引張あて材における組織学的特徴の解析
  第68回日本木材学会大会　京都
  2018年03月, ポスター発表
• Changes over time in ray parenchyma cells after wounding in Cryptomeria japonica
  IUFRO Tokyo 2017, Tokyo, Japan
  2017年10月, ポスター発表
• 露地栽培したブルーベリーの異形根性と菌根形成
  第47回根研究集会　堺
  2017年10月, 口頭発表（一般）
• Sequential analysis of rhytidome formation in Cryptomeria japonica, Quercus serrata and Cercidiphyllum japonicum
  The 9th Pacific Regional Wood Anatomy Conference（PRWAC）, Bali, Indonesia
  2017年09月, ポスター発表
• Regulation of cambial activity in trees: the role of temperature and precipitation
  The 9th Pacific Regional Wood Anatomy Conference（PRWAC）. Bali, Indonesia
  2017年09月, 口頭発表（一般）
• Tracheary elements in calli of Japanese horse chestnut (Aesculus turbinata) form perforation like structures
  The 9th Pacific Regional Wood Anatomy Conference（PRWAC）. Bali, Indonesia
  2017年09月, 口頭発表（一般）
• The pattern of location of the first earlywood vessels in the current year’s xylem in a ring-porous hardwood, Quercus serrata
  The 9th Pacific Regional Wood Anatomy Conference（PRWAC）. Bali, Indonesia
  2017年09月, 口頭発表（一般）
• Initiation of starch accumulation in ray parenchyma cells in Larix kaempferi
  The 9th Pacific Regional Wood Anatomy Conference（PRWAC）, Bali, Indonesia
  2017年09月, 口頭発表（一般）
• ショットガンプロテオミクスを用いたドロノキ放射柔細胞の放射方向におけるタンパク質変動の解析
  第81回日本植物学会大会
  2017年09月, ポスター発表
• Relationship between vacuole rupture and morphological change in nuclei in ray parenchyma cells during heartwood formation in Cryptomeria japonica
  IUFRO Conference Division 5 Forest Products
  2017年06月, ポスター発表
• Relationship between formation of earlywood vessels and growth of buds and shoots in a deciduous ring-porous hardwood, Quercus serrata
  IUFRO Conference Division 5 Forest Products
  2017年06月, その他
• Relationship between cytological changes and biosynthesis of agatharesinol in ray parenchyma in sapwood sticks of Cryptomeria japonica during cell death
  IUFRO Conference Division 5 Forest Products
  2017年06月, その他
• ブルーベリー挿し木苗の異形根性-骨格細根の発達が根系拡大に関与する
  第46回根研究集会
  2017年06月, ポスター発表
• スギから誘導したカルスのセルロースミクロフィブリルにおける長さ方向の高次構造
  第67回日本木材学会大会
  2017年03月, ポスター発表
• 交雑ポプラ培養細胞の管状要素誘導系におけるリグニン沈着過程の解析
  第67回日本木材学会大会
  2017年03月, ポスター発表
• ウルシとオオヤマレンゲの種子を用いた組織培養によるPEMs誘導に関する報告
  第67回日本木材学会大会
  2017年03月, ポスター発表
• トドマツ成熟種子由来の不定胚形成細胞を経由した植物体再生に関する研究
  第67回日本木材学会大会
  2017年03月, ポスター発表
• イチョウの二次木部における水分通道経路
  第67回日本木材学会大会
  2017年03月, ポスター発表
• サワラ樹幹に対する局所的な冷却処理やヒノキ苗木に対する水分制限処理が形成層細胞の分裂活動や木部分化過程に対して与える影響
  第67回日本木材学会大会
  2017年03月, ポスター発表
• 未成熟材形成期の枝打ちによるヒノキの仮道管長への影響
  第67回日本木材学会大会
  2017年03月, ポスター発表
• 枝打ちがヒノキの仮道管形態に与える影響の経時的観察
  第67回日本木材学会大会
  2017年03月, ポスター発表
• オニグルミの木部柔細胞における心材形成に伴う細胞内容物の変化
  第67回日本木材学会大会
  2017年03月, ポスター発表
• スギ、カツラ、コナラにおける外樹皮形成過程の経時的解析
  第67回日本木材学会大会
  2017年03月, ポスター発表
• ジベレリンおよびブラシノステロイド処理によるヤチダモおよびヤマザクラの引張あて材様組織の誘導に関する研究
  第67回日本木材学会大会
  2017年03月, ポスター発表
• スギ辺材部小片の含水率の低下が放射柔細胞の細胞死過程に与える影響
  第67回日本木材学会大会
  2017年03月, ポスター発表
• 交雑ポプラ培養細胞を用いたin vitro管状要素誘導に合成オーキシンNAAおよび2,4-Dが及ぼす影響
  第67回日本木材学会
  2017年03月, その他
• 広葉樹環孔材および散孔材における形成層活動および二次木部形成の季節性
  第67回日本木材学会
  2017年03月, その他
• カラマツ放射柔細胞の分化過程とデンプン貯蔵機能発現の関連性
  第67回日本木材学会
  2017年03月, その他
• ドロノキ放射柔細胞の細胞死に関連するプロテアーゼの遺伝子発現解析
  第67回日本木材学会
  2017年03月, その他
• ブルーベリーの細根系における個根の異質性と菌根形成
  第45回根研究集会
  2016年09月, ポスター発表
• 交雑ポプラ培養細胞を用いたin vitro管状要素誘導へのオーキシン輸送阻害剤処理の影響
  第66回日本木材学会大会
  2016年03月, その他
• The effects of defoliation and auxin transport inhibitor, N-(1-Naphthyl)phthalamic acid (NPA) on cambial reactivation and formation of earlywood tracheids in locally heated stems of Abies homolepis seedlings
  第66回日本木材学会大会
  2016年03月, その他
• エスレルおよびジャスモン酸メチル処理がウルシ樹皮における樹脂道形成に与える影響
  第66回日本木材学会大会
  2016年03月, その他
• ドロノキ木部放射柔細胞の放射方向におけるタンパク質変動のショットガンプロテオーム解析
  第66回日本木材学会大会
  2016年03月, その他
• ウルシとオオヤマレンゲの種子を用いた組織培養による植物体再生
  第66回日本木材学会大会
  2016年03月, ポスター発表
• コブシおよびシデコブシの種子由来の不定胚形成細胞を経由した植物体再生における受粉およびオーキシン濃度の影響
  第66回日本木材学会大会
  2016年03月, ポスター発表
• ポプラ培養細胞の管状要素分化誘導系を用いたリグニン沈着過程に関する研究
  第66回日本木材学会大会
  2016年03月, ポスター発表
• ハリエンジュ樹幹に対する植物ホルモン塗布処理が道管配列パターン形成に与える影響
  第66回日本木材学会大会
  2016年03月, ポスター発表
• 間伐および枝打ちがヒノキの肥大成長と仮道管の形態に与える影響
  第66回日本木材学会大会
  2016年03月, ポスター発表
• ヒノキの樹幹に対する局所的冷却処理および加温処理が形成層活動に与える影響
  第66回日本木材学会大会
  2016年03月, ポスター発表
• 木部構造の異なる広葉樹の傷害組織における着色物質の局在
  第66回日本木材学会大会
  2016年03月, ポスター発表
• ブラシノステロイド生合成阻害剤がヤマザクラの引張あて材形成に与える影響
  第66回日本木材学会大会
  2016年03月, ポスター発表
• スギ辺材部小片を用いた異なる温度条件における放射柔細胞の細胞死過程の解析
  第66回日本木材学会大会
  2016年03月, ポスター発表
• スギ放射柔細胞の細胞死過程における液胞の崩壊と核の形態変化
  第66回日本木材学会大会
  2016年03月, ポスター発表
• Analysis of heartwood formation by fluorescence microspectroscopy
  International Microscopy Workshop on Plant Sciences 2015
  2015年10月, 口頭発表（招待・特別）
• Analysis of localization of heartwood substances in the pith region of a hardwood, Robinia pseudoacacia var. inermis, by fluorescence microspectroscopy
  The second East-Asia Microscopy Conference
  2015年10月, ポスター発表
• Local expression of genes encoding tubulin isotypes in the cambium of Cryptomeria japonica
  International Symposium on Wood Science and Technology 2015 (IAWPS 2015)
  2015年03月16日, その他
• Cambial reactivation and xylem differentiation induced by localized heating of stems in conifer sawara (Chamaecyparis pisifera) trees
  International Symposium on Wood Science and Technology 2015 (IAWPS 2015)
  2015年03月16日, その他
• Effect of partial desiccation on induction of secondary xylem like tracheary element from cultured cells of hybrid poplar
  International Symposium on Wood Science and Technology 2015 (IAWPS 2015)
  2015年03月16日, その他
• Cell biological analysis of the death of long-lived ray parenchyma cells
  International Symposium on Wood Science and Technology 2015 (IAWPS 2015)
  2015年03月16日, 口頭発表（一般）
• The effects of localized heating to dormant stems on formation of the earlywood vessels in deciduous ring-porous hardwood, Quercus serrata
  International Symposium on Wood Science and Technology 2015 (IAWPS 2015)
  2015年03月16日, ポスター発表
• Morphological changes and disappearance of nuclei in ray parenchyma cells during heartwood formation in Cryptomeria japonica
  International Symposium on Wood Science and Technology 2015 (IAWPS 2015)
  2015年03月16日, ポスター発表
• カラマツ心材成分の堆積に関する組織化学的研究
  第65回日本木材学会大会
  2015年03月, その他
• トチノキ培養細胞中のせん孔様の構造をもつ管状要素の解析
  第65回日本木材学会大会
  2015年03月, その他
• コナラ苗木樹幹に対する摘芽処理およびオーキシン輸送阻害剤塗布処理が道管形成に与える影響
  第65回日本木材学会大会
  2015年03月, その他
• スギ木部放射柔細胞を用いた二次代謝を伴う細胞死誘導系における細胞内容物の経時変化
  第65回日本木材学会大会
  2015年03月, その他
• 担子菌 Coprinopsis cinerea におけるセシウムの吸収-蓄積にカリウムおよびカルシウムが与える影響
  第65回日本木材学会大会
  2015年03月, ポスター発表
• ポプラの培養細胞由来の管状要素誘導系を用いたリグニンの沈着過程に関する研究
  第65回日本木材学会大会
  2015年03月, ポスター発表
• Relationship of cambial reactivation and xylem differentiation with temperature in conifer sawara (Chamaecyparis pisifera) trees under natural condition
  第65回日本木材学会大会
  2015年03月, ポスター発表
• チャンチンモドキの不定胚形成細胞を経由した増殖系の確立
  第65回日本木材学会大会
  2015年03月, ポスター発表
• タイサンボクおよびオオヤマレンゲの組織培養による植物体再生に関する研究
  第65回日本木材学会大会
  2015年03月, ポスター発表
• シデコブシおよび人工授粉を施したホオノキの未成熟種子由来の不定胚形成細胞を経由した植物体再生に関する研究
  第65回日本木材学会大会
  2015年03月, ポスター発表
• ホオノキの器官培養およびmagnolol生産に関する研究
  第65回日本木材学会大会
  2015年03月, ポスター発表
• 樹脂生産量の異なるウルシ樹皮の組織構造の観察
  第65回日本木材学会大会
  2015年03月, ポスター発表
• ジベレリン生合成阻害剤がヤマザクラおよびホオノキの引張あて材形成に与える影響
  第65回日本木材学会大会
  2015年03月, ポスター発表
• アカマツおよびモミ放射柔細胞の放射方向における細胞内容物の変化
  第65回日本木材学会大会
  2015年03月, ポスター発表
• アカマツとモミの放射柔細胞の二次壁形成および細胞死過程における微小管挙動に関する研究
  第65回日本木材学会大会
  2015年03月, ポスター発表
• スギおよびヒノキ放射柔細胞における液胞の生体染色条件の検討
  第65回日本木材学会大会
  2015年03月, ポスター発表
• 傷害部における仮道管の脱水がスギの放射柔細胞の内容物の変化に与える影響
  第65回日本木材学会大会
  2015年03月, ポスター発表
• Cell biological studies on cell death in secondary xylem
  Eco-materials research in Japan - Applications for nanocellulose, new fungal enzymes and physiological control
  2015年01月12日, 口頭発表（招待・特別）
• Changes in cellular contents of ray parenchyma cells during heartwood formation in Cryptomeria japonica
  The 6th International Symposium of Indonesian Wood Research Society (IWoRS)
  2014年11月13日, ポスター発表
• Differences in the timing of disappearance among cellular contents during cell death of ray parenchyma in Abies sachalinensis
  The 6th International Symposium of Indonesian Wood Research Society (IWoRS)
  2014年11月12日, その他
• Property of woody biomass -the role of cell death in xylem-
  Invited seminar at Faculty of forestry, Gadjah Mada University
  2014年11月, 口頭発表（招待・特別）
• ポプラ放射柔細胞におけるxylem cysteine peptidaseの遺伝子発現解析
  第78回日本植物学会大会
  2014年09月14日, ポスター発表
• 広葉樹環孔材コナラにおける当年最初の孔圏道管形成位置
  第64回日本木材学会大会
  2014年03月, その他
• Earlier changes in cambial cells prior to cambial reactivation and xylem differentiation induced by localized heating of stems in sawara (Chamaecyparis obtusa)
  第64回日本木材学会大会
  2014年03月, その他
• ポプラXylem Cysteine Peptidaseの細胞内局在および放射柔細胞における遺伝子発現解析
  第64回日本木材学会大会
  2014年03月, その他
• スギの傷害心材形成時の脱水範囲および放射柔細胞の内容物の変化に関する研究
  第64回日本木材学会大会
  2014年03月, ポスター発表
• ヒノキにおける圧縮あて材細胞間隙の形成過程および水分通道機能に関する研究
  第64回日本木材学会大会
  2014年03月, ポスター発表
• コナラ苗木における孔圏道管の水分通道開始過程
  第64回日本木材学会大会
  2014年03月, ポスター発表
• スギの樹幹に対する局所的冷却処理が形成層分裂活動と師部および木部細胞の分化に与える影響
  第64回日本木材学会大会
  2014年03月, ポスター発表
• サワラとアカマツの樹幹に対する局所的冷却処理および遮光処理が形成層活動と木部分化過程に与える影響
  第64回日本木材学会大会
  2014年03月, ポスター発表
• 交雑ポプラ培養細胞からの管状要素分化に対する乾燥処理の影響
  第64回日本木材学会大会
  2014年03月, ポスター発表
• トガサワラ培養細胞を用いた管状要素誘導に関する研究
  第64回日本木材学会大会
  2014年03月, ポスター発表
• モクレン科樹木の培養細胞におけるmagnolol生産に関する研究
  第64回日本木材学会大会
  2014年03月, ポスター発表
• マンサク（Hamamelis japonica）の異なる植物器官からのカルス誘導および増殖条件の検討
  第64回日本木材学会大会
  2014年03月, その他
• コブシおよびシデコブシの種子由来の不定胚形成細胞を経由した植物体再生に関する研究
  第64回日本木材学会大会
  2014年03月, その他
• タイサンボクおよびオオヤマレンゲの組織培養による植物体再生に関する研究
  第64回日本木材学会大会
  2014年03月, その他
• 開放系大気CO2増加環境下で長期間生育させたブナ樹幹の道管に関する解剖学的研究
  第64回日本木材学会大会
  2014年03月, その他
• スギの心材形成に伴う放射柔細胞の細胞死過程における細胞内容物の変化
  第64回日本木材学会大会
  2014年03月, その他
• The effects of localized heating and disbudding on the earlywood vessel formation in a deciduous ring-porous wood (Quercus serrata)
  8th Pacific Regional Wood Anatomy Conference (PRWAC) & Annual meeting of International Academy of Wood Science (IAWS) 2013
  2013年10月, その他
• Stored starch contents and living wood fibers on the formation of tension wood in inclined Acacia mangium seedlings
  8th Pacific Regional Wood Anatomy Conference (PRWAC) & Annual meeting of International Academy of Wood Science (IAWS) 2013
  2013年10月, その他
• Dynamic behavior of microtubules in differentiating secondary xylem like tracheary elements in vitro
  8th Pacific Regional Wood Anatomy Conference (PRWAC) & Annual meeting of International Academy of Wood Science (IAWS) 2013
  2013年10月, その他
• Disappearance of organelles during cell death of ray parenchyma in Abies sachalinensis
  8th Pacific Regional Wood Anatomy Conference (PRWAC) & Annual meeting of International Academy of Wood Science (IAWS) 2013, Nanjing, China
  2013年10月, その他
• 水稲品種リーフスターとリーフスター/タカナリ交雑後代における強稈性に関与する稈の構造的特性
  日本作物学会第235回講演会
  2013年03月, その他
• PNAプローブを用いた蛍光in situ ハイブリダイゼーションによる木片中の木材腐朽菌の検出法の構築
  第63回日本木材学会大会
  2013年03月, その他
• 圧縮あて材の細胞間隙形成過程に関する研究
  第63回日本木材学会大会
  2013年03月, ポスター発表
• ヒノキ苗木におけるジャスモン酸メチルにより誘導される傷害樹脂道の形成過程の解析
  第63回日本木材学会大会
  2013年03月, ポスター発表
• ホオノキ（Magnolia obovata）培養細胞におけるフェニルアラニン添加のmagnolol生産に与える影響
  第63回日本木材学会大会
  2013年03月, ポスター発表
• ウルシ科6種におけるカルスおよび多芽体形成二関する培養条件の検討
  第63回日本木材学会大会
  2013年03月, ポスター発表
• オオヤマレンゲの組織培養による植物体再生に関する研究
  第63回日本木材学会大会
  2013年03月, ポスター発表
• トガサワラ培養細胞を用いた管状要素誘導系に関する研究
  第63回日本木材学会大会
  2013年03月, ポスター発表
• ドロノキ培養細胞からの管状要素誘導に関する研究
  第63回日本木材学会大会
  2013年03月, ポスター発表
• サワラ、アカマツ、ケヤキの樹幹に対する局所的冷却および遮光処理が木部細胞の形態に与える影響
  第63回日本木材学会大会
  2013年03月, ポスター発表
• スギの傷害心材形成過程に伴う木部柔細胞の細胞死過程に関する研究
  第63回日本木材学会大会
  2013年03月, ポスター発表
• 交雑ポプラ培養細胞から誘導された管状要素の有縁壁孔形成過程における表層微小管の経時観察
  第63回日本木材学会大会
  2013年03月, その他
• 休眠期の落葉性広葉樹環孔材コナラ苗木に対する樹幹への局所的加温処理および摘芽処理が孔圏道管形成に与える影響
  第63回日本木材学会大会
  2013年03月, その他
• 細胞死誘導系を用いたスギおよびカラマツ放射柔細胞の細胞死過程の経時的解析
  第63回日本木材学会大会、盛岡
  2013年03月, その他
• Cell death in long-lived ray parenchyma cells during heartwood formation in conifers
  International Seminar for BK21 program in Bioenergy Research Center in Chonnam National University, Gwangju, Korea
  2013年02月, 口頭発表（招待・特別）
• Analysis of mechanism of absorption and adsorption of aerosol in trees
  International Symposium on Aerosols in East Asia and Their impacts on Plants and Human Health
  2012年11月, ポスター発表
• PNAプローブを用いた蛍光in situ ハイブリダイゼーションによる木材中の菌糸体の可視化
  日本きのこ学会第16回大会
  2012年09月, その他
• 樹木葉面に暴露した硫酸アンモニウム粒子の可視化
  第29回エアロゾル科学・技術研究討論会
  2012年08月, その他
• In vitro induction of secondary xylem like tracheary elements from hybrid poplar cultured cells
  IUFRO Conference Division 5 Forest Products
  2012年07月, ポスター発表
• Growth eccentricity and tension wood formation in Acacia mangium seedlings at different angles of inclination
  IUFRO Conference Division 5 Forest Products
  2012年07月, ポスター発表
• The process of vessel formation in relation to leaf phenology and water conduction in deciduous hardwood species
  IUFRO Conference Division 5 Forest Products
  2012年07月, その他
• Regulation of seasonal changes in cambial activity in trees
  IUFRO Conference Division 5 Forest Products
  2012年07月, その他
• Initiation of cell death in parenchyma cells related with heartwood formation in the pith region of branches in Robinia pseudoacacia L. var. inermis
  IUFRO Conference Division 5 Forest Products, Estril, Portgul
  2012年07月, その他
• PNAプローブを用いた蛍光in situ ハイブリダイゼーションによる木材腐朽菌の検出
  日本木材保存協会第28回年次大会
  2012年05月, その他
• スギの培養細胞由来の管状要素誘導に関する研究
  第62回日本木材学会大会、札幌
  2012年03月, ポスター発表
• スギおよびメタセコイヤにおける師部貯蔵デンプンの量的変動の解析
  第62回日本木材学会大会、札幌
  2012年03月, ポスター発表
• 異なるユーカリ属の師部における組織構造および貯蔵物質の量的変動の解析
  第62回日本木材学会大会、札幌
  2012年03月, ポスター発表
• ウラジロモミ苗木における局所的冷却処理および遮光処理の木部形成に与える影響
  第62回日本木材学会大会、札幌
  2012年03月, ポスター発表
• チークとコナラを用いた形成層の加齢による道管内腔径の変動パターンの樹幹軸方向での比較
  第62回日本木材学会大会、札幌
  2012年03月, ポスター発表
• 人為的に傾斜させたアカマツ苗木の圧縮あて材形成過程の解析
  第62回日本木材学会大会、札幌
  2012年03月, ポスター発表
• Induction of latewood formation and earlier cambial dormancy during active seasons of cambium by localized cooling in conifers
  第62回日本木材学会大会、札幌
  2012年03月, その他
• The effect of gibberellin and gibberellin biosynthesis inhibitors on the anatomical structure of wood in inclined Acacia mangium seedlings
  第62回日本木材学会大会、札幌
  2012年03月, その他
• 樹木の葉面に人工的に暴露した硫酸アンモニウム乾燥粒子の局在解析
  第62回日本木材学会大会、札幌
  2012年03月, その他
• GFP-MAP4遺伝子の導入によるポプラ培養細胞からの管状要素分化過程における表層微小管の観察
  第62回日本木材学会大会、札幌
  2012年03月, その他
• トドマツ放射柔細胞の細胞死過程における細胞小器官の動的変化
  第62回日本木材学会大会、札幌
  2012年03月, その他
• イメージング技術を用いた樹木二次木部細胞の細胞死発現機構に関する研究
  第123回日本森林学会大会テーマ別シンポジウム.ポストゲノム時代における新たな森林研究の展開（3）
  2012年03月, 口頭発表（招待・特別）

委員歴

• 日本木材学会
  常任理事
  自 201906, 至 202106
• 日本木材学会
  編集委員
  自 201704, 至 201903
• 東京都稲城市
  稲城市環境審議会委員長
  自 20240401, 至 20260331
• 東京都稲城市
  稲城市環境審議会委員
  自 20200106, 至 20200331

所属学協会

• 日本木材学会
• 日本植物学会
• 生態工学会
• 国際木材解剖学会

受賞

• 社団法人日本木材学会
  第26回日本木材学会奨励賞
  樹木の木部放射組織における細胞死発現機構に関する研究
  2015年03月17日