研究者データベース
馬谷 千恵UMATANI Chieｳﾏﾀﾆ ﾁｴ
| ■所属部署名 | 農学研究院 応用生命化学部門 | |||
| ■職名 | 助教 | |||
Last Updated :2023/09/06
業績情報
氏名・連絡先
氏名
ｳﾏﾀﾆ ﾁｴ, 馬谷 千恵, UMATANI Chie
主たる所属・職名
農学研究院 応用生命化学部門, 助教
学位
科学研究費助成事業
- 基盤研究(B)
卵黄栄養依存から採餌行動への移行を司る魚類神経メカニズムの解明
自 2020年, 至 2023年 - 挑戦的研究（萌芽）
オスにおける生殖腺刺激ホルモン非依存的な性成熟の神経メカニズムの謎に迫る
自 2022年, 至 2024年
論文
- Molecular encoding and synaptic decoding of context during salt chemotaxis in C. elegans
Hiroki, Shingo; Yoshitane, Hikari; Mitsui, Hinako; Sato, Hirofumi; Umatani, Chie; Kanda, Shinji; Fukada, Yoshitaka; Iino, Yuichi
NATURE COMMUNICATIONS
NATURE PORTFOLIO
Animals navigate toward favorable locations using various environmental cues. However, the mechanism of how the goal information is encoded and decoded to generate migration toward the appropriate direction has not been clarified. Here, we describe the mechanism of migration towards a learned concentration of NaCl in Caenorhabditis elegans. In the salt-sensing neuron ASER, the difference between the experienced and currently perceived NaCl concentration is encoded as phosphorylation at Ser65 of UNC-64/Syntaxin 1 A through the protein kinase C(PKC-1) signaling pathway. The phosphorylation affects basal glutamate transmission from ASER, inducing the reversal of the postsynaptic response of reorientation-initiating neurons (i.e., from inhibitory to excitatory), guiding the animals toward the experienced concentration. This process, the decoding of the context, is achieved through the differential sensitivity of postsynaptic excitatory and inhibitory receptors. Our results reveal the mechanism of migration based on the synaptic plasticity that conceptually differs from the classical ones. The nematode C. elegans moves around to find an optimal environment. This work demonstrates how it can detect and move towards a previously learned salinity using the salt-sensing neuron ASER.
2022年05月27日, 研究論文（学術雑誌）, 共同, 13, 1, DOI(公開)(r-map) - Neuropeptide FF indirectly affects testicular morphogenesis and functions in medaka
Tomihara, Soma; Ikegami, Kana; Shimomai, Rinko; Umatani, Chie
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
NATL ACAD SCIENCES
Testicular morphogenesis and functions are considered to be under the control of neural and endocrine systems. However, the available literature is mainly limited to mammals, and it remains unclear how they are regulated in teleost species. Here, we demonstrated that neuropeptide FF (NPFF) in the brain is responsible for the follicle-stimulating hormone expression in the pituitary, which facilitates the testicular morphogenesis and androgen synthesis, and subsequently contributes to successful spermatogenesis. The present findings give us important insights into the neuroendocrine regulatory mechanisms underlying the testicular morphogenesis and functions in teleosts.
2022年11月07日, 研究論文（学術雑誌）, 共同, 119, 46, 0027-8424, DOI(公開)(r-map) - In vitro and in vivo gene transfer in the cloudy catshark Scyliorhinus torazame
Fujimori, Chika; Umatani, Chie; Chimura, Misaki; Ijiri, Shigeho; Bando, Hisanori; Hyodo, Susumu; Kanda, Shinji
DEVELOPMENT GROWTH & DIFFERENTIATION
WILEY
Cartilaginous fishes have various unique physiological features such as a cartilaginous skeleton and a urea-based osmoregulation strategy for adaptation to their marine environment. Also, because they are a sister group of bony vertebrates, understanding their unique features is important from an evolutionary perspective. However, genetic engineering based on gene functions as well as cellular behavior has not been effectively utilized in cartilaginous fishes. This is partly because their reproductive strategy involves internal fertilization, which results in difficulty in microinjection into fertilized eggs at the early developmental stage. Here, to identify efficient gene transfer methods in cartilaginous fishes, we examined the effects of various methods both in vitro and in vivo using the cloudy catshark, a candidate model cartilaginous fish species. In all methods, green fluorescent protein (GFP) expression was used to evaluate exogenous gene transfer. First, we examined gene transfer into primary cultured cells from cloudy catshark embryos by lipofection, polyethylenimine (PEI) transfection, adenovirus infection, baculovirus infection, and electroporation. Among the methods tested, lipofection, electroporation, and baculovirus infection enabled the successful transfer of exogenous genes into primary cultured cells. We then attempted in vivo transfection into cloudy catshark embryos by electroporation and baculovirus infection. Although baculovirus-injected groups did not show GFP fluorescence, electroporation successfully introduced GFP into muscle cells. Furthermore, we succeeded in GFP transfer into adult tissues by electroporation. The in vitro and in vivo gene transfer methods that worked in this study may open ways for genetic manipulation including knockout experiments and cellular lineage analysis in cartilaginous fishes.
2022年12月, 研究論文（学術雑誌）, 共同, 64, 9, 0012-1592, DOI(公開)(r-map), 558, 565 - Estrogen upregulates the firing activity of hypothalamic gonadotropin-releasing hormone (GnRH1) neurons in the evening in female medaka
Ikegami, Kana; Kajihara, Sho; Umatani, Chie; Nakajo, Mikoto; Kanda, Shinji; Oka, Yoshitaka
JOURNAL OF NEUROENDOCRINOLOGY
WILEY
The reproductive function of vertebrates is regulated by the hypothalamic-pituitary-gonadal axis. In sexually mature females, gonadotropin-releasing hormone (GnRH) neurons in the preoptic area (POA) are assumed to be responsible for a cyclic large increase in GnRH release, the GnRH surge, triggering a luteinizing hormone (LH) surge, which leads to ovulation. Precise temporal regulation of the preovulatory GnRH/LH surge is important for successful reproduction because ovulation should occur after follicular development. The time course of the circulating level of estrogen is correlated with the ovulatory cycle throughout vertebrates. However, the neural mechanisms underlying estrogen-induced preovulatory GnRH surge after folliculogenesis still remain unclear, especially in non-mammals. Here, we used a versatile non-mammalian model medaka for the analysis of the involvement of estrogen in the regulation of POA-GnRH (GnRH1) neurons. Electrophysiological analysis using a whole brain-pituitary in vitro preparation, which maintains the hypophysiotropic function of GnRH1 neurons intact, revealed that 17 beta-estradiol (E-2) administration recovers the ovariectomy-induced lowered GnRH1 neuronal activity in the evening, indicating the importance of E-2 for upregulation of GnRH1 neuronal activity. The importance of E-2 was also confirmed by the fact that GnRH1 neuronal activity was low in short-day photoperiod-conditioned females (low E-2 model). However, E-2 failed to upregulate the firing activity of GnRH1 neurons in the morning, suggesting the involvement of additional time-of-day signal(s) for triggering GnRH/LH surges at an appropriate timing. We also provide morphological evidence for the localization of estrogen receptor subtypes in GnRH1 neurons. In conclusion, we propose a working hypothesis in which both estrogenic and time-of-day signals act in concert to timely upregulate the firing activity of GnRH1 neurons that trigger the GnRH surge at an appropriate timing in a female-specific manner. This neuroendocrinological mechanism is suggested to be responsible for the generation of ovulatory cycles in female teleosts in general.
2022年04月, 研究論文（学術雑誌）, 共同, 34, 4, 0953-8194, DOI(公開)(r-map)
メディア報道
- 東大と東京農工大、メダカにおいて精巣の形態・機能維持に重要な脳内因子を発見
発表者の一人として馬谷 千恵（研究当時:東京大学大学院理学系研究科生物科学専攻 助教/現:東京農工大学大学院農学研究院 助教）さんが紹介される。
日本経済新聞
自 2022年11月08日, 至 2022年11月08日 - メダカ精巣の形態・機能を維持 東大が脳内ペプチド発見
東京大学大学院理学系研究科の馬谷千恵助教（現・東京農工大学大学院農学研究院助教）らの研究グループは、脳内で発現する神経ペプチドの一種「ニューロペプチドＦＦ（ＮＰＦＦ）」の機能をゲノム編集技術により喪失させたメダカのオスの精巣形態・機能を詳細に解析したと紹介される。
科学新聞／WEB
自 2022年11月25日, 至 2022年11月25日