村上　智亮MURAKAMI Tomoakiムラカミ　トモアキ

業績情報

氏名・連絡先

• 氏名

  ムラカミ　トモアキ, 村上　智亮, MURAKAMI Tomoaki

• 生年

  1985

• eメールアドレス

  mrkmtcc.tuat.ac.jp

• 個人ホームページ

  http://www.tuat.ac.jp/~tatlvt/

主たる所属・職名

• 農学研究院　動物生命科学部門, 准教授

その他の所属

• 農学部　共同獣医学科
• 農学府

経歴

• 東京農工大学
  農学研究院獣医毒性学研究室
  准教授
  自 2018年11月01日
• 東京農工大学
  農学研究院獣医毒性学研究室
  助教
  自 2013年11月01日, 至 2018年10月31日
• -
  日本学術振興会特別研究員（PD）
  自 2013年10月01日, 至 2013年10月31日
• -
  日本学術振興会特別研究員（DC2）
  自 2013年04月01日, 至 2013年09月30日
• -
  帯広食肉衛生検査所･とちく検査員（非常勤）
  自 2010年07月12日, 至 2010年09月30日

学歴

• 岐阜大学大学院
  連合獣医学研究科
  獣医学専攻
  至 2013年09月30日, 修了, 博士
• 帯広畜産大学
  畜産学部
  獣医学科
  至 2010年03月31日, 卒業

学位

• 博士（獣医学）

免許・資格

• 日本獣医病理学専門家協会（JCVP）会員資格
  2016年04月01日
• 獣医師免許
  2010年04月01日

教育・研究活動状況

• 分野横断的アプローチによるアミロイドーシスの病態理解

研究分野

• A189　ライフサイエンス, A42020　獣医学
• A189　ライフサイエンス, A49030　実験病理学

研究キーワード

• アミロイドーシス
• 家禽疾病
• 疾患モデル動物

研究テーマ

• 食事中のアミロイドを介したAAアミロイド症異種間伝達リスクに関する研究
  自 201311
• 鳥類AAアミロイド症に関する研究
  自 201311
• 自然発生疾患に関する病理学的研究
  自 201507
• Laser microdissection-LC/MS/MS法を用いた動物アミロイドの同定
  自 201607

担当授業科目

• 獣医倫理
  2023年, 専門科目等
• 獣医学概論
  2023年, 専門科目等
• 国際応用動物学Ⅰ
  2022年, 専門科目等
• 研究デザイン演習(変則通年集中(～7月)・集中・農学府（4年制博士課程）)
  2022年, 専門科目等
• 研究プレゼンテーション演習(変則通年・集中・農学府（4年制博士課程）)
  2022年, 専門科目等
• 動物・植物科学(3学期・木2・農学部)
  2022年, 専門科目等
• 毒性学実習(3学期・水3～4・農学部)
  2022年, 専門科目等
• 農学基礎ゼミ(1学期・木2・農学部)
  2022年, 教養科目等

科学研究費助成事業

• 基盤研究(B)
  ニホンリスを病態モデルとしたフィブリノゲンα鎖アミロイドーシス発生原理の解明
  自 2023年, 至 2025年
• 挑戦的研究（萌芽）
  構造特異的自家蛍光を利用したアミロイドの非標識検出およびタイピング
  自 2022年, 至 2023年
• 基盤研究(B)
  自己乳化現象の制御による「超低密度」多孔質粒子の作製と経肺投与DDSへの応用
  自 2022年, 至 2022年
• 基盤研究(B)
  自己乳化現象の制御による「超低密度」多孔質粒子の作製と経肺投与DDSへの応用
  自 2021年, 至 2021年
• 若手研究
  質量分析を用いた新規動物アミロイドーシス同定への挑戦
  自 2020年, 至 2021年
• 基盤研究(B)
  自己乳化現象の制御による「超低密度」多孔質粒子の作製と経肺投与DDSへの応用
  自 2020年, 至 2020年
• 基盤研究(B)
  血清アミロイドA3が関与する新たな粘膜免疫機構と腸管での感染防御機能の解明
  自 2019年, 至 2019年
• 基盤研究(B)
  血清アミロイドA3が関与する新たな粘膜免疫機構と腸管での感染防御機能の解明
  自 2018年, 至 2018年
• 若手研究(B)
  環境中のアミロイドを介した鳥類AAアミロイド症の伝播性に関する研究
  自 2017年, 至 2018年
• 基盤研究(B)
  血清アミロイドA3が関与する新たな粘膜免疫機構と腸管での感染防御機能の解明
  自 2017年, 至 2017年
• 基盤研究（B）
  血清アミロイドA3が関与する新たな粘膜免疫機構と腸管での感染防御機能の解明
  自 2016年, 至 2016年

論文

• Znrf3 exon 2 deletion mice do not recapitulate congenital adrenal hypoplasia
  2024年09月, 研究論文（学術雑誌）, DOI(公開)(r-map)
• Alpha-synuclein expression in GnRH neurons of young and old bovine hypothalami
  2024年09月, 研究論文（学術雑誌）, DOI(公開)(r-map)
• Eggshell membrane and its major component lysozyme and ovotransferrin enhance the secretion of decorin as an endogenous antifibrotic mediator from lung fibroblasts and ameliorate bleomycin-induced pulmonary fibrosis
  2024年09月, 研究論文（学術雑誌）, DOI(公開)(r-map)
• Meningoencephalitis with malacia caused by Sarcocystis calchasi in a rock pigeon in Japan
  2024年08月, 研究論文（学術雑誌）, DOI(公開)(r-map)
• Studies on the potential risk of amyloidosis from exposure to cultured fibril from silk fibroin
  2024年07月, 研究論文（学術雑誌）, DOI(公開)(r-map)
• Identification of apolipoprotein E-derived amyloid within cholesterol granulomas of leopard geckos (Eublepharis macularius)
  2024年06月, 研究論文（学術雑誌）, DOI(公開)(r-map)
• Systemic AL kappa chain amyloidosis in a captive Bornean orangutan (Pongo pygmaeus)
  2024年06月, 研究論文（学術雑誌）, DOI(公開)(r-map)
• A case report of cryptococcosis in a captive Cape hyrax (Procavia capensis)
  2024年04月, 研究論文（学術雑誌）, DOI(公開)(r-map)
• Apolipoprotein C-III amyloidosis in white lions (Panthera leo)
  Kobayashi N, Iwaide S, Fukui H, Une Y, Itoh Y, Hisada M, Murakami T
  Veterinary Pathology
  2024年02月, 研究論文（学術雑誌）, DOI(公開)(r-map)
• Senile plaques and phosphorylated tau deposition in a super-aged rhesus monkey (Macaca mulatta)
  Iwaide, Susumu; Nakayama, Yutaro; Chambers, James K.; Uchida, Kazuyuki; Nakagawa, Daisuke; Yamanashi, Yumi; Bando, Haruna; Murakami, Tomoaki
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  The brain of a rhesus monkey that died at 43 years of age with symptoms of suspected cognitive dysfunction was analyzed. pathological analyses revealed characteristic Alzheimer's disease-related lesions: the aggregation of amyloid beta (A beta) in the form of senile plaques and phosphorylated tau proteins. We also revealed that A beta 43, which is prone to aggregation and toxicity in humans, is involved in senile plaques in the brain of the rhesus monkey, as well as several other A beta species. Comparative studies of neuropathology using aged nonhuman primates lack behavioral descriptions compared to human medicine. This case report showed behavioral abnormalities and the detailed pathological changes that may have caused it in a super-aged rhesus monkey.
  2023年12月, 研究論文（学術雑誌）, 共同, 85, 12, 0916-7250, DOI(公開)(r-map), 1296, 1300
• Identification of calcitonin receptor-stimulating peptide 1-derived amyloid in a feline C-cell carcinoma
  Murakami T, Kobayashi N, Iwaide S, Itoh Y, Hisada M, Izawa T, Kuwamura M
  Amyloid
  2023年11月, 研究論文（学術雑誌）, DOI(公開)(r-map)
• Label-free autofluorescence and hyperspectral imaging of cerebral amyloid β lesions in aged squirrel monkeys
  Oba R, Ujike N, Ono Y, Okano T, Murakami T.
  Journal of Veterinary Diagnostic Investigation
  2023年10月, 研究論文（学術雑誌）, 36, 1, DOI(公開)(r-map), 41, 45
• Fibrinogen Aα-chain amyloidosis outbreaks in Japanese squirrels (Sciurus lis): a potential disease model
  Iwaide, Susumu; Ito, Nanami; Ogino, Shiori; Kobayashi, Natsumi; Koyama, Satoshi; Hisamoto, Shumpei; Kondo, Hirotaka; Itoh, Yoshiyuki; Hisada, Miki; Hoshino, Yuki; Nakagawa, Daisuke; Matsubara, Yuki; Nakamura, Shin-ichi; Satoh, Hiroshi; Shibuya, Hisashi; Murakami, Tomoaki
  JOURNAL OF PATHOLOGY
  WILEY
  Fibrinogen A alpha-chain amyloidosis is a hereditary systemic amyloidosis characterized by glomerular amyloid depositions, which are derived from the fibrinogen A alpha-chain variant in humans. Despite its unique pathology, the pathogenic mechanisms of this disease are only partially understood. This is in part because comparative pathological studies on fibrinogen A alpha-chain amyloidosis are currently unavailable as there is a lack of reported cases in animals other than humans. In this study, mass spectrometry-based proteomic analyses of Japanese squirrels (Sciurus lis) that died in five Japanese zoos showed that they developed glomerular-associated fibrinogen A alpha-chain amyloidosis with an extremely high incidence rate (29/38 cases, 76.3%). The condition was found to be age-dependent in the Japanese squirrels, with 89% of individuals over 4 years of age affected. Mass spectrometry revealed that the C-terminal region of the fibrinogen A alpha-chain was involved in amyloidogenesis in Japanese squirrels as well as humans. No gene variations were identified between amyloid-positive and amyloid-negative squirrels, which contrasted with the available data for humans. The results indicate that fibrinogen A alpha-chain amyloidosis is a senile amyloidosis in Japanese squirrels. The results have also provided comparative pathological support that the amyloidogenic C-terminal region of the fibrinogen A alpha-chain is involved in the characteristic glomerular pathology, regardless of the animal species. This study elucidates the potential causes of death in Japanese squirrels and will contribute to future comparative pathological studies of fibrinogen A alpha-chain amyloidosis. (c) 2023 The Pathological Society of Great Britain and Ireland.
  2023年08月07日, 研究論文（学術雑誌）, 共同, 0022-3417, DOI(公開)(r-map)
• Fibrinogen Aα-chain amyloidosis outbreaks in Japanese squirrels (Sciurus lis): a potential disease model
  Iwaide S, Ito N, Ogino S, Kobayashi N, Koyama S, Hisamoto S, Kondo H, Itoh Y, Hisada M, Hoshino Y, Nakagawa D, Matsubara Y, Nakamura S, Satoh H, Shibuya H, Murakami T.
  The Journal of Pathology
  2023年08月, 研究論文（学術雑誌）, 共同, DOI(公開)(r-map)
• Apolipoprotein A-IV amyloidosis in a cotton-top tamarin (Saguinus oedipus)
  Masoud, Niki Sedghi; Iwaide, Susumu; Itoh, Yoshiyuki; Hisada, Miki; Une, Yumi; Murakami, Tomoaki
  Amyloid
  TAYLOR & FRANCIS LTD
  2023年07月03日, 研究論文（学術雑誌）, 共同, 30, 3, 1350-6129, DOI(公開)(r-map), 348, 350
• Pathological Characterization of Spontaneous AA Amyloidosis in Microminipigs
  Inoue, Misaki; Miyazaki, Shinya; Kobayashi, Natsumi; Kangawa, Akihisa; Murakami, Tomoaki
  TOXICOLOGIC PATHOLOGY
  SAGE PUBLICATIONS INC
  The minipig has been used as a non-rodent species in nonclinical toxicology studies, but little is known about amyloid A (AA) amyloidosis in this species. Among domestic pigs, reports of AA amyloidosis have been limited to animals with mutations in the N-terminal residue of serum AA (SAA), which is thought to be a primary etiological factor. In this study, we histologically examined 26 microminipigs aged 0.6 to 10 years and observed amyloid deposition in one 0.6-year-old and six 5-year-old or older microminipigs. The amyloid deposits were identified as AA based on mass spectrometry (MS) and immunohistochemistry (IHC). The 0.6-year-old microminipig showed severe deposition in the renal cortex and spleen, whereas 5-year-old or older animals had severe deposition in the renal medulla. MS and IHC detected serum amyloid P-component (SAP) in amyloid deposits in older animals but not in a 0.6-year-old animals. Based on the proteomic analysis and gene sequencing, amino acid mutations of SAA, previously found in domestic pigs, were not involved in the pathogenesis of AA amyloidosis in microminipigs. This study demonstrates that microminipigs with wild-type SAA develop AA amyloidosis and presents the possibility that differences in the environment surrounding amyloid, such as SAP, may influence differences in the pathological phenotype.
  2023年07月, 研究論文（学術雑誌）, 共同, 51, 5, 0192-6233, DOI(公開)(r-map), 257, 263
• Local administration of amyloid enhancing factor initiates in situ amyloid A deposition followed by systemic lesions in mice
  Experimental Animals
  2023年05月, 研究論文（学術雑誌）, DOI(公開)(r-map)
• Identification of novel amyloidosis in dogs: α-S1-casein acquires amyloidogenicity in mammary tumor by overexpression and N-terminal truncation
  Murakami, Tomoaki; Kaku, Toshisuke; Tsukakoshi, Kaori; Iwaide, Susumu; Itoh, Yoshiyuki; Hisada, Miki; Nomura, Kohji; Kubo, Rikako; Ikebukuro, Kazunori; Sassa-O'Brien, Yukiko; Kametani, Fuyuki
  VETERINARY PATHOLOGY
  SAGE PUBLICATIONS INC
  Mammary tumor-associated amyloidosis (MTAA) in dogs is characterized by amyloid deposition in the stroma of mammary adenoma or carcinoma; however, the amyloid precursor protein remains unknown. We attempted to identify an amyloid precursor protein and elucidated its etiology by characterizing 5 cases of canine MTAA. Proteomic analyses of amyloid extracts from formalin-fixed paraffin-embedded specimens revealed alpha-S1-casein (CASA1) as a prime candidate and showed the N-terminal truncation of canine CASA1. Both immunohistochemistry and immunoelectron microscopy showed that amyloid deposits or fibrils in MTAA cases were positive for CASA1. Reverse transcription-polymerase chain reaction and quantitative polymerase chain reaction revealed the complete mRNA sequence encoding CASA1, whose expression was significantly higher in the amyloid-positive group. The recombinant protein of the N-terminal-truncated canine CASA1 and the synthetic peptides derived from canine and human CASA1 formed amyloid-like fibrils in vitro. Structural prediction suggested that the N-terminal region of CASA1 was disordered. Previously, full-length CASA1 was reported to inhibit the amyloidogenesis of other proteins; however, we demonstrated that CASA1 acquires amyloidogenicity via excessive synthesis followed by truncation of its disordered N-terminal region. By identifying a novel in vivo amyloidogenic protein in animals and revealing key mechanistic details of its associated pathology, this study provides valuable insights into the integrated understanding of related proteopathies.
  2023年03月, 研究論文（学術雑誌）, 共同, 60, 2, 0300-9858, DOI(公開)(r-map), 203, 213
• Identification of ameloblastin as an amyloid precursor protein of amyloid-producing ameloblastoma in dogs and cats
  Masoud, Niki Sedghi; Iwaide, Susumu; Itoh, Yoshiyuki; Hisada, Miki; Harada, Tomoyuki; Murakami, Tomoaki
  VETERINARY SCIENCES
  MDPI
  Simple Summary Amyloid-producing ameloblastoma (APAB) is a rare type of odontogenic tumor found in cats and dogs. This study was conducted to explore this condition further by using proteomic analysis based on mass spectrometry in dogs and cats and identified ameloblastin as an amyloid precursor protein. This study also showed that the N-terminal region of ameloblastin may be involved in the amyloidogenesis. Amyloid-producing ameloblastoma (APAB) is characterized by abundant amyloid deposits in ameloblastoma, but the amyloid precursor protein is unknown. To explore this, we conducted histopathologic and proteomic analyses on formalin-fixed and paraffin-embedded samples from five cases of APAB (three dogs and two cats). Histologically, the samples exhibited a proliferation of the odontogenic epithelium, with moderate to severe interstitial amyloid deposits. By using Congo red and polarized light, the amyloid deposits were found to show characteristic birefringence. Amyloid deposits were dissected from tissue sections and analyzed by LC/MS/MS, and high levels of ameloblastin were detected in all tissues. Mass spectrometry also revealed that the N-terminal region of ameloblastin is predominantly present in amyloid deposits. Immunohistochemistry was performed using two anti-ameloblastin (N terminal, middle region) antibodies and showed that amyloid deposits were positive for ameloblastin N terminal but negative for ameloblastin middle region. These results suggest that ameloblastin is the amyloid precursor protein of APABs in dogs and cats, and the N-terminal region may be involved in the amyloidogenesis of ameloblastin.
  2023年02月, 研究論文（学術雑誌）, 共同, 10, 2, DOI(公開)(r-map)
• Local administration of amyloid enhancing factor initiates in situ amyloid A deposition followed by systemic lesions in mice
  Iwaide, Susumu; Oba, Ryohei; Kobayashi, Natsumi; Murakami, Tomoaki
  EXPERIMENTAL ANIMALS
  INT PRESS EDITING CENTRE INC
  Amyloid A (AA) amyloidosis is experimentally transmissible in some animal species, such as mice and chickens. While the spleen is important as the initial deposition site in the transmission of AA amyloidosis, it is not essential for establishing the transmission, and its role is not precisely understood. In this study, to clarify why the spleen is the first site of deposition in transmissible AA amyloidosis, we administered amyloid enhancing factor, which is AA fibrils extracted from AA amyloidosis affected mouse to local organs (liver, spleen, kidney, stomach wall, and Peyer's patches), to tail vein and into peritoneum; then compared the amyloid distribution. Interestingly, initial amyloid deposition was observed at the administration site in each administered organ, not just the spleen. Furthermore, the amount of amyloid deposition in intra-organ administration groups was larger than that of the intravenous or intraperitoneal administration groups. This study indicates that locally exposed AEF initiates in situ amyloid deposition, from which amyloid deposition spreads throughout the body.
  2023年02月, 研究論文（学術雑誌）, 共同, 72, 2, 1341-1357, DOI(公開)(r-map), 218, 223
• Keratinic amyloid deposition in canine hair follicle tumors
  Kobayashi, Kyoko; Iwaide, Susumu; Sakai, Hiroki; Kametani, Fuyuki; Murakami, Tomoaki
  VETERINARY PATHOLOGY
  SAGE PUBLICATIONS INC
  Keratinic primary localized cutaneous amyloidosis is a disease in humans; however, no similar condition has been reported in animals. This study aimed to investigate cutaneous keratinic amyloid deposition in dogs and elucidate its etiology. Canine hair follicle tumor tissues were histopathologically analyzed. Immunohistochemistry and mass spectrometry-based proteomic analyses were performed to identify precursor protein candidates. Structural prediction and in vitro fibrillization analyses were conducted to determine the amyloidogenic region and gene sequencing analysis was performed to assess mutations. Of the 266 samples, 16 had amyloid deposition. Amyloid deposits were found in the stroma of tumors and in the margins of keratin debris and around normal hair follicles. Cytokeratin 5 (CK5) was identified as a precursor protein candidate. C-terminal truncation of CK5 was observed in amyloid deposits, and the truncation sites varied depending on the deposition pattern. There was a significantly higher incidence of amyloid deposition in Shiba dogs, and CK5 amino acid polymorphisms were identified in these dogs. A part of the C-terminal region of both canine and human CK5 exhibited highly amyloidogenic properties in vitro. This study revealed the existence of cutaneous keratinic amyloid deposition in animals and identified CK5 as an amyloid precursor protein, providing novel insights into understanding the etiology of cutaneous amyloidosis.
  2023年01月, 研究論文（学術雑誌）, 共同, 60, 1, 0300-9858, DOI(公開)(r-map), 60, 68
• Keratinic amyloid deposition in canine hair follicle tumors
  Veterinary Pathology
  2022年10月11日, 研究論文（学術雑誌）, DOI(公開)(r-map)
• Silk fibroin-based vascular repairing sheet with angiogenic-promoting activity of SVVYGLR peptide regenerated the damaged vascular in rats
  Shimada, Kazumi; Honda, Tadakatsu; Kato, Kounosuke; Hori, Ryosei; Ujike, Naoki; Uemura, Akiko; Murakami, Tomoaki; Kitpipatkun, Pitipat; Nakazawa, Yasumoto; Tanaka, Ryou
  JOURNAL OF BIOMATERIALS APPLICATIONS
  SAGE PUBLICATIONS LTD
  Medical sheets are useful in surgically repair vascular disease. To avoid long-term side effects, they are to be replaced with regenerated tissue after implantation. Silk fibroin is a fibrous protein secreted by silkworm. The advantage of silk fibroin is its biocompatibility and has been used as regenerative artificial materials. The problem of its biodegradability is that the effect is time consuming. In this study, SVVYGLR peptide was used to expect promoting cell migration and accelerating the biodegradation of silk fibroin. Silk fibroin and polyurethane-based medical sheets with or without SVVYGLR peptide were implanted in rat abdominal aorta (silk fibroin/polyurethane/SVVYGLR peptide versus silk fibroin/polyurethane). The result of histological evaluation indicated that the new cell layer created under both sheets was composed of endothelial cells, smooth muscle, and fibroin in both sheets and similar to a native vessel. Both sheets did not show any excessive inflammation or calcification, and moderate biodegradability was observed. The decrease of silk fibroin indicated the biodegradability of all sheets. Silk fibroin/polyurethane/SVVYGLR peptide had many small vessels in the regenerated tissue than silk fibroin/polyurethane. This appearance indicated that SVVYGLR peptide promoted the angiogenesis in the regenerative tissue. This study suggested that SVVYGLR peptide could give the angiogenic-promoting activity to silk fibroin-based vascular repairing sheet.
  2022年07月, 研究論文（学術雑誌）, 共同, 37, 1, 0885-3282, DOI(公開)(r-map), 3, 11
• Systemic amyloidosis derived from EFEMP1 in a captive Tsushima leopard cat
  Miyazaki, Shinya; Kobayashi, Yuki; Kametani, Fuyuki; Kobayashi, Kyoko; Iwaide, Susumu; Yanai, Tokuma; Murakami, Tomoaki
  VETERINARY PATHOLOGY
  SAGE PUBLICATIONS INC
  In animals, most cases of systemic amyloidosis are of amyloid A type, and the other types of systemic amyloidoses are rare. This study analyzed systemic amyloidosis in a 15-year-old female Tsushima leopard cat. Amyloid deposits strongly positive for Congo red staining were observed in the arterial walls as well as the interstitium in multiple organs. Mass spectrometry-based proteomic analysis with laser microdissection of amyloid deposits identified epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) as a prime amyloidogenic protein candidate. Immunohistochemistry showed that the amyloid deposits were positive for the N-terminal region of EFEMP1. From these results, the present case was diagnosed as EFEMP1-derived amyloidosis. It is the first such case in an animal. EFEMP1-derived amyloidosis in humans has recently been reported as a systemic amyloidosis, and it is known as an age-related venous amyloidosis. The present case showed different characteristics from human EFEMP1-derived amyloidosis, including the amyloid deposition sites and the amyloidogenic region of the EFEMP1 protein, suggesting a different pathogenesis between Tsushima leopard cat and human EFEMP1-derived amyloidosis.
  2022年01月, 研究論文（学術雑誌）, 共同, 59, 1, 0300-9858, DOI(公開)(r-map), 152, 156
• Intrinsic fluorescence-based label-free detection of bovine amyloid A amyloidosis
  Ujike, Naoki; Iwaide, Susumu; Ono, Yuki; Okano, Takayuki; Murakami, Tomoaki
  JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
  SAGE PUBLICATIONS INC
  Amyloidosis is diagnosed by the histologic detection of amyloid deposits; however, this method has limitations such as a prolonged diagnosis time and the need for histologic proficiency. We aimed to develop a rapid and simple method for diagnosing amyloidosis by targeting amyloid-specific endogenous fluorescence, which has not been reported previously, to our knowledge. Fluorescence fingerprint analysis of amyloid extracts and tissue homogenates derived from amyloid A (AA) amyloidosis-affected cattle exhibited a specific intrinsic fluorescence pattern. Furthermore, principal component analysis using analytical data revealed that AA could be identified by peaks near lambda(ex) 350 nm and lambda(em) 430 nm. Fluorescence spectrometry analysis using tissue homogenates, which does not require special histochemical staining, enables the rapid detection of bovine AA.
  2022年01月, 研究論文（学術雑誌）, 共同, 34, 1, 1040-6387, DOI(公開)(r-map), 130, 132
• Systemic amyloidosis derived from EFEMP1 in a captive Tsushima leopard cat
  Miyazaki S, Kobayashi Y, Kametani F, Kobayashi K, Iwaide S, Yanai T, Murakami T
  Veterinary Pathology
  2021年11月11日, 研究論文（学術雑誌）, 共同, 59, 1, DOI(公開)(r-map), 152, 156
• Intrinsic fluorescence–based label-free detection of bovine amyloid A amyloidosis.
  Ujike N, Iwaide S, Ono Y, Okano T, Murakami T
  Journal of Veterinary Diagnostic Investigation
  2021年10月05日, 研究論文（学術雑誌）, 共同, 34, 1, DOI(公開)(r-map), 130, 132
• Silk fibroin/polyurethane patch implantation in hyperglycemic rat model
  Kitpipatkun, Pitipat; Sutummaporn, Kripitch; Kato, Konosuke; Murakami, Tomoaki; Kobayashi, Kyoko; Nakazawa, Yasumoto; Tanaka, Ryou
  JOURNAL OF BIOMATERIALS APPLICATIONS
  SAGE PUBLICATIONS LTD
  Purpose To understand the complication and histopathological characteristics between the Silk Fibroin/Polyurethanes (SF/PU) and the host response, and to unveil the compatibility of the patch in diabetes individuals. Methods Rats were divided into DM and control (CT) groups, and the DM group was induced with streptozotocin. All groups underwent the SF/PU patch implantation in the abdominal aorta, and the implanted patches were evaluated at one, two, three, and four weeks after implantation. Results DM group had more fibrosis formation and a delayed endothelialization compared to the CT group. There was no evidence of chronic inflammation in both DM and CT groups. Conclusions Fibrosis in hyperglycemic individuals could promote the formation of new vascular structures in the implanted patch such as endothelial and vascular smooth muscle cells. In summary, the SF/PU patch was no serious complications when implanted under hyperglycemia, and the patch was suitable to implant in diabetes mellitus.
  2021年10月, 研究論文（学術雑誌）, 共同, 36, 4, 0885-3282, DOI(公開)(r-map), 701, 713
• Proteopathic lesions in the brain of a super-aged chimpanzee (Pan troglodytes)
  Iwaide, Susumu; Yanai, Tokuma; Murakami, Tomoaki
  JOURNAL OF MEDICAL PRIMATOLOGY
  WILEY
  The brain of a chimpanzee estimated to be 68 years old, the oldest reported so far, has been examined. Pathological analyses revealed the formation of mild tau-positive neuritic clusters and cytoplasmic alpha-synuclein aggregates, in addition to severe cerebral amyloid angiopathy and diffuse plaques, but no tangle lesions were observed.
  2021年08月, 研究論文（学術雑誌）, 共同, 50, 4, 0047-2565, DOI(公開)(r-map), 222, 224
• Effect of edaravone against cisplatin-induced chronic renal injury
  Koike, Natsumi; Sasaki, Ayaka; Murakami, Tomoaki; Suzuki, Kazuhiko
  DRUG AND CHEMICAL TOXICOLOGY
  TAYLOR & FRANCIS LTD
  Cisplatin has been widely used as an anticancer agent for a wide range of tumors, but it had nephrotoxicity that was mainly caused by oxidative stress. Edaravone, a free radical scavenger, has reportedly been validated to have a protective effect against renal injury induced by reactive oxygen species. However, most of these reports are against AKI, and few studies have examined the effect of chronic renal injury. In this study, we investigate the effect of edaravone on cisplatin nephropathy in the chronic phase. Twenty-five male Wistar rats were divided into five groups: control, cisplatin, cisplatin + edaravone 1 mg kg(-1), cisplatin + edaravone 10 mg kg(-1), and cisplatin + edaravone 100 mg kg(-1). Edaravone was administrated intraperitoneally every other day for 5 weeks, starting 1 week before cisplatin administration (6 mg kg(-1), i.p.). As a result, proximal tubule injury, interstitial fibrosis, and mononuclear cell infiltration were ameliorated histologically in the group of rats treated with high edaravone dose. In the cisplatin group, the number of alpha-SMA-, CD68-, and CD3-positive cells increased markedly compared with the Control group, but these numbers were significantly decreased by higher doses of co-administered edaravone. While there was no clear mRNA expression variation in antioxidant enzymes, the apoptosis-promoting factors, caspase8, were markedly reduced in the high-dose edaravone co-administration group compared with the cisplatin group. In conclusion, our results suggested that cisplatin-induced renal injury in the chronic phase was ameliorated by edaravone.
  2021年07月04日, 研究論文（学術雑誌）, 共同, 44, 4, 0148-0545, DOI(公開)(r-map), 437, 446
• Species-barrier on the cross-species oral transmission of bovine AA amyloidosis in mice
  Iwaide, Susumu; Ujike, Naoki; Kobayashi, Kyoko; Sassa, Yukiko; Murakami, Tomoaki
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  In AA amyloidosis, cross-species oral transmission has been demonstrated in several animal models. While it is known that the transmission efficiency of AA amyloidosis between different species is lower than that among the same species, the mechanism of this species-barrier is unclear. In this study, we found at first that mice orally given a large amount of bovine AA simultaneously with inflammatory stimulation did not develop AA amyloidosis. Therefore, we hypothesized that the low efficiency of the cross-species oral transmission of AA amyloidosis might be due to the low absorption rate in Peyer's patches. To evaluate the hypothesis, we next investigated whether bovine AA was taken up by Peyer's patches and translocated to other organs in vivo and ex vivo models. The direct absorption of bovine AA by Peyer's patches was not observed. Besides, translocation of bovine AA to the mesenteric lymph nodes, spleen, liver, or kidney was not observed except the mesenteric lymph node of a single mouse. Thus, absorption of bovine AA by Peyer's patches occurred much less efficiently in mouse models of cross-species oral transmission of AA amyloidosis. The present study suggests that the less efficient amyloid uptake by Peyer's patches may be involved in the species-barrier of oral transmission of AA amyloidosis.
  2021年06月10日, 研究論文（学術雑誌）, 共同, 83, 6, 0916-7250, DOI(公開)(r-map), 962, 967
• Oxazolone-induced gastrointestinal disorders enhance the oral transmission of AA amyloidosis in mice
  Kobayashi, Hiroto; Iwaide, Susumu; Ujike, Naoki; Murakami, Tomoaki
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  Amyloid A (AA) amyloidosis is a lethal disease characterized by systemic AA amyloid deposition, and is reported in many animal species. Despite experiments have shown that AA amyloidosis can be transmitted orally, horizontal transmission and cross-species transmission are concerns, the transmission mechanism has been unknown. In this study, we examined the oral transmission efficiency of AA amyloidosis using oxazolone-induced gastrointestinal disorder mice. As a result, the upper or lower gastrointestinal disorder groups developed more severe amyloid deposition in systemic tissues than the group without gastrointestinal disorders. The results of this study suggest that gastrointestinal damage promotes the oral transmission of AA amyloidosis.
  2021年06月09日, 研究論文（学術雑誌）, 共同, 83, 6, 0916-7250, DOI(公開)(r-map), 935, 939
• In vitroandex vivoexpression of serum amyloid A3 in mouse lung epithelia
  Kawasaki, Haruka; Murakami, Tomoaki; Badr, Yassien; Kamiya, Sato; Shimizu, Kaori; Okada, Ayaka; Inoshima, Yasuo
  EXPERIMENTAL LUNG RESEARCH
  TAYLOR & FRANCIS INC
  Background and Purpose Serum amyloid A (SAA), an acute-phase protein whose level tracks infection and inflammation, is the precursor protein of amyloid A (AA) fibrils that is thought to cause AA amyloidosis in human and animals. SAA protein has several isoforms based on the difference of amino acid sequence, such as SAA1 to SAA4 in mice. AA fibrils are associated with chronic inflammation and are mainly originated from SAA1 produced in the liver. SAA3 reportedly contributes to the innate immune response in epithelia; however, little is known about its role at the lung epithelia. Therefore, we investigated SAA3 expression in the lung epithelium activated by bacterial antigens. Materials and Methods The expressions of SAA3 and SAA1 mRNA were investigated using quantitative real-time PCR,in vitrousing mouse Clara (Club) cells andex vivousing surgically removed mouse lungs, after their stimulation by using either lipopolysaccharide (LPS), the major outer membranous antigen of gram-negative bacteria, or lipoteichoic acid (LTA), the major outer membranous antigen of gram-positive bacteria. In addition, SAA3 and SAA1/2 proteins in treated lung samples were detected by immunohistochemistry (IHC). Results SAA3 mRNA expression increased in cells and lungs treated with either LPS or LTA. SAA3 mRNA was more sensitively expressed in LPS than LTA treatment. In contrast, SAA1 mRNA expression did not increase by either LPS or LTA treatment. Furthermore, SAA3 mRNA expression increased in a dose-dependent manner in cells treated with tumor necrosis factor-alpha. By IHC, SAA3 protein was highly expressed in the luminal side of the bronchial epithelium, while SAA1/2 was not expressed. Conclusion These results obtained fromin vitroandex vivoexperiments suggest that SAA3 plays an important role in the innate immune response to bacterial infection in the lung epithelia.
  2020年10月20日, 研究論文（学術雑誌）, 共同, 46, 9, 0190-2148, DOI(公開)(r-map), 352, 361
• AA Amyloid Deposition in the Central and Peripheral Nervous Systems in Flamingos
  Ono, Ayumi; Nakayama, Yumi; Inoue, Maki; Yanai, Tokuma; Murakami, Tomoaki
  VETERINARY PATHOLOGY
  SAGE PUBLICATIONS INC
  AA amyloidosis is characterized by amyloid deposition in systemic organs, but amyloid deposition in the central nervous system (CNS) or peripheral nervous system (PNS) is rare. In this study, AA amyloidosis was observed in 31 of 48 flamingos that died at a Japanese zoo. Almost all cases developed AA amyloidosis secondary to inflammatory diseases such as enteritis. Affected flamingos had AA amyloid deposition around blood vessels in periventricular white matter of the brain and in peripheral nerves. In addition, cerebral A beta amyloidosis was observed in one of the 31 cases with AA amyloidosis. In conclusion, flamingos in the zoo commonly developed systemic amyloidosis with frequent amyloid deposition in the CNS and PNS, which seems to be a unique distribution in this avian species. Comparative pathological analyses in flamingos may help elucidate the pathogenesis of amyloid neuropathy.
  2020年09月, 研究論文（学術雑誌）, 共同, 57, 5, 0300-9858, DOI(公開)(r-map), 700, 705
• Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats
  Kadota, Ayumi; Iwaide, Susumu; Miyazaki, Shinya; Mitsui, Ikki; Machida, Noboru; Murakami, Tomoaki
  VETERINARY PATHOLOGY
  SAGE PUBLICATIONS INC
  Amyloidosis is classified according to the amyloid precursor protein, and accurate diagnosis of the amyloidosis type may guide appropriate treatment. Immunohistochemistry and Congo red staining are the most frequently used methods used to distinguish types of amyloidosis, but problems with specificity and sensitivity indicate the need for an alternative diagnostic method. In this study, we evaluated laser microdissection-liquid chromatography-tandem mass spectrometry (LMD-LC-MS/MS) for the diagnosis of amyloid light-chain (AL) amyloidosis in animals. Plasmacytomas with amyloid deposits from 15 dogs and 2 cats were subjected to Congo red staining with or without potassium permanganate pretreatment, immunohistochemistry for kappa and lambda light chains, and LMD-LC-MS/MS. Congo red staining was diagnostic in 12 of 17 cases based on resistance to potassium permanganate pretreatment, but in 5 of 17 cases the pretreatment unexpectedly reduced Congo red staining or abrogated the birefringence and a definitive diagnosis could not be reached. Immunohistochemistry detected kappa or lambda light chains in 6 of 17 cases. With LMD-LC-MS/MS, immunoglobulin lambda light chain was detected in all 17 cases. The amyloid signature proteins ApoA-I, ApoA-IV, and ApoE were detected in 9, 1, and 3 of the 15 canine cases by LMD-LC-MS/MS, but not in the feline cases. In conclusion, LMD-LC-MS/MS consistently determined the amyloid type in all examined specimens, while Congo red staining after potassium permanganate treatment and immunohistochemistry were less sensitive tests.
  2020年09月, 研究論文（学術雑誌）, 共同, 57, 5, 0300-9858, DOI(公開)(r-map), 658, 665
• Differential expression of serum amyloid A1 and A3 in bovine epithelia
  Murata, Eriko; Kozaki, Satoi; Murakami, Tomoaki; Shimizu, Kaori; Okada, Ayaka; Ishiguro, Naotaka; Inoshima, Yasuo
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  Serum amyloid A (SAA) is both an amyloidogenic protein of amyloid A amyloidosis and an acute phase protein in most animal species. Although SAA isoforms, such as SAA1, 2, 3, and 4, have been identified in cattle, their biological functions are not completely understood. Previous studies using mice indicated that SAA3 mRNA expression increased by stimulation with Escherichia coli and lipopolysaccharide (LPS) in colonic epithelial cells, and subsequently the SAA3 protein enhanced the expression of mucin2 (MUC2) mRNA, which is the major component of the colonic mucus layer. These results suggest that SAA3 plays a role in host innate immunity against bacterial infection in the intestine. In this study, a novel anti-bovine SAA3 monoclonal antibody was produced and SAA3 expression levels in bovine epithelia were examined in vitro and in vivo using real-time PCR and immunohistochemistry (IHC). SAA3 mRNA expression, but not that of SAM, was enhanced by LPS stimulus in bovine small intestinal and mammary glandular epithelial cells in vitro. Moreover, in bovine epithelia (small intestine, mammary gland, lung, and uterus) obtained from four Holstein dairy cows from a slaughterhouse, SAA3 mRNA expression was higher than that of SA41. Furthermore, using IHC, SAA3 protein expression was observed in bovine epithelia, whereas SAA1 protein was not. These results suggest that in cattle, SAA3 plays an immunological role against bacterial infection in epithelial tissues, including the small intestine, mammary gland, lung, and uterus.
  2020年06月, 研究論文（学術雑誌）, 共同, 82, 6, 0916-7250, DOI(公開)(r-map), 764, 770
• Silk Fibroin-based vascular repairing sheet with angiogenic-promoting activity of SVVYGLR peptide regenerated the damaged vascular in Rats
  Shimada K, Honda T, Kato K, Hori R, Ujike N, Uemura A, Murakami T, Kitpipatkun P, Nakazawa Y, Tanaka R
  Journal of Biomaterials Applications
  2020年05月01日, 研究論文（学術雑誌）, 共同, -, -, DOI(公開)(r-map), -, -
• Amyloid Signature Proteins in Feline Amyloidosis
  Miyazaki, S.; Kadota, A.; Mitsui, I; Murakami, T.
  JOURNAL OF COMPARATIVE PATHOLOGY
  ELSEVIER SCI LTD
  In human amyloidoses, amyloid signature proteins (ASPs), such as serum amyloid P component (SAP) and apolipoprotein E (ApoE), are deposited in tissues together with amyloid fibrils and are implicated in the pathogenesis of amyloidosis. Few reports describe ASPs in animals. In this study, we examined feline amyloidosis and performed immunohistochemical and proteomic analyses of SAP, ApoE, apolipoprotein A-I (ApoAI) and apolipoprotein A-IV (ApoAIV). Ten cases of systemic amyloidosis, three cases of amyloid-producing odontogenic tumour and three cases of islet amyloidosis were used for immunohistochemistry (IHC) and/or proteomic analyses. IHC showed that ApoE was present in amyloid deposits in all samples. ApoAI and ApoAIV differed in the degree of co-deposition with amyloid depending on the type of amyloid and the affected organ. SAP was negative in all amyloid deposits. Proteomic analysis showed that ApoE was present in all samples, but ApoAI and ApoAIV were detected only in some samples and SAP was not detected in any samples. The observation that ApoE was detected in all types of amyloid suggests the involvement of ApoE in the development of feline amyloidosis. ASPs in feline amyloidosis are significantly different from those in human amyloidosis, suggesting that the involvement of ASPs in the pathological condition differs between animal species. (C) 2020 Elsevier Ltd. All rights reserved.
  2020年05月, 研究論文（学術雑誌）, 共同, 177, 0021-9975, DOI(公開)(r-map), 10, 17
• 鳥類アミロイドAアミロイドーシスの病理と伝播
  村上智亮
  鶏病研究会報
  2020年03月01日, 研究論文（学術雑誌）, 単独, 55, 4, 131, 135
• Multicystic peritoneal tumor in two layer hens
  Yoshida, Keiko; Miyoshi, Tomoyuki; Murakami, Tomoaki
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  In chicken, peritoneal cystic lesions have not been clearly categorized. In this study, diffuse peritoneal multiple cysts were observed in two layer hens. The cysts in the serosa were lined with single layers of squamous or cuboidal cells. The papillary proliferations of columnar cells were also observed in one case. The smooth muscle layer or mass were observed around the cysts in both cases. The cystic lining cells were positive for pan-cytokeratin, vimentin, S100 and Wilms tumor 1. Ultrastructurally, they had sparsely microvilli on the luminal surface. The histological results indicated the present cases were multicystic mesothelioma, but also had characteristics of Mullerian epithelium. This is the first report describing the detailed pathological feature of unique multicystic tumor in chicken.
  2020年03月, 研究論文（学術雑誌）, 共同, 82, 3, 0916-7250, DOI(公開)(r-map), 294, 298
• Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein
  Murakami, Tomoaki; Noguchi, Keiichi; Hachiya, Naomi; Kametani, Fuyuki; Tasaki, Masayoshi; Nakaba, Satoshi; Sassa, Yukiko; Yamashita, Taro; Obayashi, Konen; Ando, Yukio; Hamamura, Masao; Kanno, Takeshi; Kawasako, Kazufumi
  AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
  TAYLOR & FRANCIS LTD
  Amyloidosis is an extremely rare event in rats. In this study, we report that lipopolysaccharide binding protein (LBP) is the most likely amyloidogenic protein in rat mammary amyloidosis. Histologically, corpora amylacea (CA) and stromal amyloid (SA) were observed in rat mammary glands, and needle-shaped amyloid (NA) was also observed on the surface or gap of CA and SA. Following surveillance in aged rats, NA was observed in 62% of mammary tumours, 25% of male mammary glands and 83% of female mammary glands. Proteomic analysis showed that lactadherin was a major constitutive protein of CA and SA, and both were positive following immunohistochemistry with anti-lactadherin antibodies. In the same analysis, LBP was detected as a prime candidate protein in NA, and NA was positive following immunohistochemistry and immunoelectron microscopy with anti-LBP antibody. Furthermore, synthetic peptides derived from rat LBP formed amyloid fibrils in vitro. Overall, these results provide evidence that LBP is an amyloid precursor protein of NA in rat mammary glands.
  2020年01月02日, 研究論文（学術雑誌）, 共同, 27, 1, 1350-6129, DOI(公開)(r-map), 25, 35
• Experimental model of oral transmissible AA amyloidosis in quails
  Miyoshi, Tomoyuki; Ono, Ayumi; Shimada, Kazumi; Murakami, Tomoaki
  AVIAN PATHOLOGY
  TAYLOR & FRANCIS LTD
  In poultry and zoo birds, mass outbreaks of amyloid A (AA) amyloidosis are often reported, and horizontal transmission is considered as one of the causes. However, oral transmission of avian AA amyloidosis in nature has been unclear. In order to clarify the horizontal transmission of avian AA amyloidosis, basic research using an appropriate oral transmission model is necessary. In this study, we developed an oral transmission model of AA amyloidosis using quails, and assessed the oral transmission efficiency of AA amyloidosis in quails and mice. Young quails, adult quails, and young mice received inflammatory stimulation with lipopolysaccharide; simultaneously, homogeneous amyloid fibrils were orally or intravenously administered. By histological examination, induction of amyloidosis by oral or intravenous administration of amyloid was confirmed in all species. Furthermore, both quail and murine AA amyloidosis were orally transmitted in a dose-dependent manner. These results support the possibility of horizontal transmission of avian AA amyloidosis in nature. This model will be able to contribute to the elucidation of spontaneous horizontal transmission of avian AA amyloidosis in the future.
  2019年11月02日, 研究論文（学術雑誌）, 共同, 48, 6, 0307-9457, DOI(公開)(r-map), 521, 527
• Effect of edaravone against cisplatin-induced chronic renal injury
  Koike, Natsumi; Sasaki, Ayaka; Murakami, Tomoaki; Suzuki, Kazuhiko
  DRUG AND CHEMICAL TOXICOLOGY
  TAYLOR & FRANCIS LTD
  Cisplatin has been widely used as an anticancer agent for a wide range of tumors, but it had nephrotoxicity that was mainly caused by oxidative stress. Edaravone, a free radical scavenger, has reportedly been validated to have a protective effect against renal injury induced by reactive oxygen species. However, most of these reports are against AKI, and few studies have examined the effect of chronic renal injury. In this study, we investigate the effect of edaravone on cisplatin nephropathy in the chronic phase. Twenty-five male Wistar rats were divided into five groups: control, cisplatin, cisplatin + edaravone 1 mg kg(-1), cisplatin + edaravone 10 mg kg(-1), and cisplatin + edaravone 100 mg kg(-1). Edaravone was administrated intraperitoneally every other day for 5 weeks, starting 1 week before cisplatin administration (6 mg kg(-1), i.p.). As a result, proximal tubule injury, interstitial fibrosis, and mononuclear cell infiltration were ameliorated histologically in the group of rats treated with high edaravone dose. In the cisplatin group, the number of alpha-SMA-, CD68-, and CD3-positive cells increased markedly compared with the Control group, but these numbers were significantly decreased by higher doses of co-administered edaravone. While there was no clear mRNA expression variation in antioxidant enzymes, the apoptosis-promoting factors, caspase8, were markedly reduced in the high-dose edaravone co-administration group compared with the cisplatin group. In conclusion, our results suggested that cisplatin-induced renal injury in the chronic phase was ameliorated by edaravone.
  2019年05月07日, 研究論文（学術雑誌）, 共同, 44, 4, 0148-0545, DOI(公開)(r-map), 437, 446
• Dimethyl fumarate ameliorates cisplatin-induced renal tubulointerstitial lesions
  Sasaki, Ayaka; Koike, Natsumi; Murakami, Tomoaki; Suzuki, Kazuhiko
  JOURNAL OF TOXICOLOGIC PATHOLOGY
  JAPANESE SOC TOXICOLOGIC PATHOLOGY
  Dimethyl fumarate (DMF) has an antioxidant effect by activating the nuclear factor erythroid 2-related transcription factor 2 (Nrf2). Cisplatin (CIS) has nephrotoxicity as a frequently associated side effect that is mainly mediated by oxidative stress. In this study, we investigated whether the DMF-mediated antioxidative mechanism activated by Nrf2 can ameliorate CIS-induced renal tubulointerstitial lesions in rats. In Experiments 1 and 2, 25 five-week-old male Wistar rats were divided into five groups: control, CIS, and 3 CIS+DMF groups (300, 1,500, and 7,500 ppm in Experiment 1; 2,000, 4,000, and 6,000 ppm in Experiment 2). Rats were fed their respective DMF-containing diet for 5 weeks. CIS was injected 1 week after starting DMF administration, and the same volume of saline was injected into the control group. CIS-induced severe tubular injury, such as necrosis and degeneration in the outer segment of the outer medulla, was inhibited in the 7,500 ppm DMF group and ameliorated in all DMF groups in Experiment 2. Increased interstitial mononuclear cell infiltration and increased Sirius red-positive areas were also observed in CIS-administered groups, and these increases tended to be dose-dependently inhibited by DMF co-administration in Experiments 1 and 2. The numbers of alpha-smooth muscle actin (SMA)-positive myofibroblasts, CD68-positive macrophages, and CD3-positive lymphocytes observed in the peritubular area also increased with CIS administration, and these increases were dose-dependently inhibited by DMF co-administration. Moreover, renal cortical mRNA expression of Nrf2-related genes such as NQO1 increased in DMF groups. This investigation showed that DMF ameliorates CIS-induced renal tubular injury via NQO1-mediated antioxidant mechanisms and reduces the consequent tubulointerstitial fibrosis.
  2019年04月01日, 研究論文（学術雑誌）, 共同, 32, 2, 0914-9198, DOI(公開)(r-map), 79, 89
• Solubilized eggshell membrane supplies a type III collagen-rich elastic dermal papilla
  Ohto-Fujita, Eri; Shimizu, Miho; Sano, Shoei; Kurimoto, Masashi; Yamazawa, Kai; Atomi, Tomoaki; Sakurai, Takashi; Murakami, Yoshihiko; Takami, Taku; Murakami, Tomoaki; Yoshimura, Kotaro; Hasebe, Yukio; Atomi, Yoriko
  CELL AND TISSUE RESEARCH
  SPRINGER
  Signs of aging in facial skin correlate with lifespan and chronic disease; however, the health of aging skin has not been extensively studied. In healthy young skin, the dermis forms a type III collagen-rich dermal papilla, where capillary vessels supply oxygen and nutrients to basal epidermal cells. Chicken eggshell membranes (ESMs) have been used as traditional medicines to promote skin wound healing in Asian countries for many years. Previously, we designed an experimental system in which human dermal fibroblasts (HDFs) were cultured on a dish with a solubilized ESM (S-ESM) bound to an artificial phosphorylcholine polymer; we found that genes that promoted the health of the papillary dermis, such as those encoding type III collagen, were induced in the S-ESM environment. The present study found that a gel with a ratio of 20% type III/80% type I collagen, similar to that of the baby skin, resulted in a higher elasticity than 100% type I collagen (p<0.05) and that HDFs in the gel showed high mitochondrial activity. Thus, we decided to perform further evaluations to identify the effects of S-ESM on gene expression in the skin of hairless mice and found a significant increase of type III collagen in S-ESM. Picrosirius Red staining showed that type III collagen significantly increased in the papillary dermis after S-ESM treatment. Moreover, S-ESM application significantly improved human arm elasticity and reduced facial wrinkles. ESMs may have applications in extending lifespan by reducing the loss of tissue elasticity through the increase of type III collagen.
  2019年04月01日, 研究論文（学術雑誌）, 共同, 376, 1, 0302-766X, DOI(公開)(r-map), 123, 135
• Persistent hyperplastic primary vitreous in two piglets
  Murakami, Tomoaki; Miyoshi, Tomoyuki; Takahashi, Natsumi; Kangawa, Akihisa
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  Two Large Yorkshire piglets were diagnosed as persistent hyperplastic primary vitreous (PHPV). In case 1, the white cord-like structure extending from optic disc to lens was observed in the normal-sized right eye. Case 2 showed buphthalmos of the right eye. The internal structure of the right eye was unclear due to bleeding, but a white cord-like structure was slightly observed. In both cases, histological examinations revealed the fibrovascular cord-like structure in hyaloid vitreous. The retina was detached, and dysplastic nervous tissue was observed in anterior vitreous. Immunohistochemistry using various neural markers suggested that dysplastic nervous tissue was derived from the detached neural retina. By the characteristic macroscopic and histopathological features, both cases were diagnosed as PHPV. To our knowledge, this is the first report of swine PHPV.
  2019年03月01日, 研究論文（学術雑誌）, 共同, 81, 3, 0916-7250, DOI(公開)(r-map), 357, 360
• Pleomorphic Malignant Mesothelioma in a Broiler Breeder Infected with Avian Leucosis Virus Subgroup J
  Murakami, T.; Sassa, Y.
  JOURNAL OF COMPARATIVE PATHOLOGY
  ELSEVIER SCI LTD
  Avian leucosis virus (ALV) is an oncogenic retrovirus that induces tumours including lymphoid leucosis and myeloid lcucosis. Pleomorphic malignant mesothelioma and myelocytoma, which were thought to be induced by ALV subgroup J (ALV-J)infection, were identified in a 432-day-old broiler breeder. The bird showed no clinical signs; however, at necropsy examination there were multiple nodules in the alimentary tract. Microscopical analysis showed that these consisted of pleomorphic cells and myelocyte-like cells. Immunohistochemistry revealed that the pleomorphic cells were atypical and expressed cytokeratin, vimentin, c-kit, calretinin and ALV. The myelocyte-like cells were also positive for ALV. Retroviral type C particles were observed by electron microscopy. ALV-E and ALV-J nucleotide sequences were detected in DNA extracted from formalin-fixed and paraffin wax-embedded small intestinal tissue. Based on these results, the tumours were diagnosed as pleomorphic malignant mesothelioma and myelocytoma and were thought to have been induced by ALV-J infection. This is the first report of malignant mesothelioma associated with naturally acquired ALV-J infection. (C) 2018 Elsevier Ltd. All rights reserved.
  2018年04月, 研究論文（学術雑誌）, 共同, 160, 0021-9975, DOI(公開)(r-map), 50, 55
• Inhibitory effect of propolis on the development of AA amyloidosis
  Harata, Daichi; Tsuchiya, Yuya; Miyoshi, Tomoyuki; Yanai, Tokuma; Suzuki, Kazuhiko; Murakami, Tomoaki
  JOURNAL OF TOXICOLOGIC PATHOLOGY
  JAPANESE SOC TOXICOLOGIC PATHOLOGY
  In the several types of amyloidoses, participation of oxidative stresses in the pathogenesis and the effect of antioxidants on amyloidosis have been reported. Meanwhile, the relationship between oxidative stresses and pathogenesis of amyloid A (AA) amyloidosis is still unclear. In this study, we used an antioxidant, Brazilian propolis, to investigate the inhibitory effects on AA amyloidosis. The results showed that AA deposition was inhibited by administration of propolis. Increased expression of antioxidant markers was detected in molecular biological examinations of mice treated with propolis. Although serum amyloid A (SAA) levels were strongly correlated with the immunoreactive area of AA deposits in the control group, the correlation was weaker in the propolis-treated groups. In addition, there were no changes in SAA levels between the control group and the propolis-treated groups. The results indicate that propolis, an antioxidant, may induce inhibitory effects against AA amyloidosis.
  2018年, 研究論文（学術雑誌）, 共同, 31, 2, 0914-9198, DOI(公開)(r-map), 89, 93
• 精練法の異なる家蚕シルクフィブロインの組織工学材料としての評価
  Tajiri, Hirokazu; Kubo, Honami; Murakami, Tomoaki; Yoshioka, Taiyo; Hata, Tamako; Kameda, Tsunenori; Nakazawa, Chikako T.; Tara, Shuhei; Nakazawa, Yasumoto
  KOBUNSHI RONBUNSHU
  SOC POLYMER SCIENCE JAPAN
  Non-woven fabrics made of two kinds of silk fibroin, one extracted directly from silk glands of living silkworms (silk grand fibroin; SGF) and the other regenerated from degummed silk fibroin (regenerated silk fibroin; RSF), were prepared. The secondary structure, mechanical properties and histological characters of these two kinds of fabrics were investigated and compared for biomaterial use. Although the a-sheet crystal contents of these two kinds of fabrics were the same, the breaking strength and the Young's modulus values of SGF fabric were significantly higher than those of RSF fabric. The difference of the physical properties is considered to be derived from the difference of the molecular weights of the silk fibroins because RSF degrades during the degumming process. In the histological test, SGF fabrics didn't show calcification, but phagocytosis by multinucleated giant cells was partly observed 4weeks after the implantation into subcutaneous mouse tissue. Expression level of cytokines from in vitro tests revealed that inflammation by macrophages is the same in SGF and RSF films.
  2018年, 研究論文（学術雑誌）, 共同, 75, 1, 0386-2186, DOI(公開)(r-map), 54, 60
• Silk fibroin-Pellethane (R) cardiovascular patches: Effect of silk fibroin concentration on vascular remodeling in rat model
  Chantawong, Pinkarn; Tanaka, Takashi; Uemura, Akiko; Shimada, Kazumi; Higuchi, Akira; Tajiri, Hirokazu; Sakura, Kohta; Murakami, Tomoaki; Nakazawa, Yasumoto; Tanaka, Ryou
  JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
  SPRINGER
  Life-threatening cardiovascular anomalies require surgery for structural repair with cardiovascular patches. The biomaterial patch, derived from Bombyx mori silk fibroin (SF), is used as an alternative material due to its excellent tissue affinity and biocompatibility. However, SF lacks the elastomeric characteristics required for a cardiovascular patch. In order to overcome this shortcoming, we combined the thermoplastic polyurethane, Pellethane (R) (PU) with SF to develop an elastic biocompatible patch. Therefore, the purpose of this study was to investigate the feasibility of the blended SF/PU patch in a vascular model. Additionally, we focused on the effects of different SF concentrations in the SF/PU patch on its biological and physical properties. Three patches of different compositions (SF, SF7PU3 and SF4PU6) were created using an electrospinning method. Each patch type (n = 18) was implanted into rat abdominal aorta and histopathology was assessed at 1, 3, and 6 months post-implantation. The results showed that with increasing SF content the tensile strength and elasticity decreased. Histological evaluation revealed that inflammation gradually decreased in the SF7PU3 and SF patches throughout the study period. At 6 months post-implantation, the SF7PU3 patch demonstrated progressive remodeling, including significantly higher tissue infiltration, elastogenesis and endothelialization compared with SF4PU6. In conclusion, an increase of SF concentration in the SF/ PU patch had effects on vascular remodeling and physical properties. Moreover, our blended patch might be an attractive alternative material that could induce the growth of a neo-artery composed of tissue present in native artery.
  2017年12月, 研究論文（学術雑誌）, 共同, 28, 12, 0957-4530, DOI(公開)(r-map)
• Inhibitory effect of propolis on the development of AA amyloidosis
  Harata D, Tsuchiya Y, Miyoshi T, Yanai T, Suzuki K, Murakami T
  Journal of Toxicologic Pathology
  2017年12月, 研究論文（学術雑誌）, 共同, 31, 2, DOI(公開)(r-map), 89, 93
• Spontaneous, Experimentally Induced, and Transmissible AA Amyloidosis in Japanese Quail (Coturnix japonica)
  Nakayama, Yumi; Kamiie, Junichi; Watanabe, Gen; Suzuki, Kazuhiko; Murakami, Tomoaki
  VETERINARY PATHOLOGY
  SAGE PUBLICATIONS INC
  The authors describe a spontaneous case of amyloid A (AA) amyloidosis in an adult female Japanese quail (Coturnix japonica). The bird developed AA amyloidosis secondary to chronic peritonitis caused by a Gram-negative bacillus infection. Mild amyloid deposition was also identified in the intestinal tract of apparently healthy adult individuals, suggesting that quail may develop intestinal amyloidosis with age. Based on these observations, it was hypothesized that quail can develop AA amyloidosis following inflammatory stimulation with lipopolysaccharide (LPS). Therefore, adult quail were repeatedly injected with LPS and the development of AA amyloidosis was confirmed. The amyloid deposition in this model increased when quail amyloid was intravenously injected as an amyloid-enhancing factor. The experiments were repeated with young quail, but amyloid deposits were not observed following LPS injections. However, AA amyloidosis did develop when quail amyloid was injected in addition to LPS. These results indicated that adult quail develop AA amyloidosis after inflammatory stimulation with LPS. Furthermore, quail AA amyloidosis was shown to have transmissibility regardless of age. Interestingly, the authors found that administration of chicken amyloid fibrils also induced AA amyloidosis in young quail. This is the first report of cross-species transmission of avian AA amyloidosis.
  2017年11月, 研究論文（学術雑誌）, 共同, 54, 6, 0300-9858, DOI(公開)(r-map), 912, 921
• 精練法の異なる家蚕シルクフィブロインの組織工学材料としての評価.
  田尻弘和, 久保穗菜美, 村上智亮, 吉岡太陽, 秦珠子, 亀田恒徳, 中澤千香子, 太良修平, 中澤靖元
  高分子論文集
  2017年10月, 研究論文（大学，研究機関紀要）, 共同, 75, 1, DOI(公開)(r-map), 54, 60
• The effect of β-caryophyllene on non-alcoholic steatohepatitis
  Arizuka N, Murakami T, Suzuki K
  Journal of Toxicologic Pathology
  2017年06月, 研究論文（学術雑誌）, 共同, 30, -, DOI(公開)(r-map), 263, 273
• Maternal Exposure to Valproic Acid Primarily Targets Interneurons Followed by Late Effects on Neurogenesis in the Hippocampal Dentate Gyrus in Rat Offspring
  Watanabe, Yousuke; Murakami, Tomoaki; Kawashima, Masashi; Hasegawa-Baba, Yasuko; Mizukami, Sayaka; Imatanaka, Nobuya; Akahori, Yumi; Yoshida, Toshinori; Shibutani, Makoto
  NEUROTOXICITY RESEARCH
  SPRINGER
  Valproic acid (VPA) is used to establish models of experimental autism. The present study investigated the developmental exposure effect of VPA on postnatal hippocampal neurogenesis in accordance with the exposure scheme of OECD Test Guideline 426 adopted for developmental neurotoxicity. Pregnant rats were administered drinking water containing 0, 667, or 2000 ppm VPA from gestational day 6 until day 21 post-delivery. In the subgranular zone (SGZ) and granule cell layer (GCL) of offspring, the number of granule cell lineage subpopulations remained unchanged upon weaning. However, in the hilus of the dentate gyrus, the number of reelin(+) interneurons decreased at ae667 ppm, and the number of PVALB(+) or GAD67(+) interneurons decreased at 2000 ppm. Conversely, Reln and Gad1 transcript levels increased at 2000 ppm, but Pvalb and Grin2d decreased, in the dentate gyrus. At the adult stage, PCNA(+) proliferating SGZ cells, NeuN(+) postmitotic SGZ/GCL neurons, and ARC(+) or COX2(+) GCL neurons increased at ae667 ppm. In the dentate hilus, decreases in GAD67(+) interneuron subpopulations and Grin2d transcript levels sustained at 2000 ppm. These results suggested that VPA primarily targets interneurons by developmental exposure, and this is followed by late effects on granule cell lineages, likely by influencing SGZ cell proliferation and synaptic plasticity. A reduced population of reelin(+) or PVALB(+) interneurons did not affect distribution of granule cell lineage subpopulations upon weaning. The late effect on neurogenesis, which resulted in increased GCL neurons, might be the result of a sustained decrease in GAD67(+) interneurons expressing NR2D encoded by Grin2d.
  2017年01月, 研究論文（学術雑誌）, 共同, 31, 1, 1029-8428, DOI(公開)(r-map), 46, 62
• The effect of a silk Fibroin/Polyurethane blend patch on rat Vessels
  Shimada, Kazumi; Higuchi, Akira; Kubo, Ryota; Murakami, Tomoaki; Nakazawa, Yasumoto; Tanaka, Ryou
  ORGANOGENESIS
  TAYLOR & FRANCIS INC
  Patch grafts are widely used in various kind of vascular surgeries such as detect repair or dilation of vascular stenosis. Expanded polytetrafluoroethylene (ePTFE) patches are flexible and handle well, but have shown problems with calcification as they are non-bioabsorbable and therefore permanently remain in the body. It is important to develop an alternative biocompatible patch. Silk fibroin (SF) was developed as a biocompatible material, but it lacks of the elasticity required for surgery as a patch. Polyurethane (PU) is also a well-known elastomer so this study focused on the SF and the PU blend materials with a weight ratio of 5:5 (SF/PU). To evaluate the SF/PU patch, the patches were implanted into the abdominal aortas of rats, using the ePTFE patch in the control group. Because it was more flexible the SF/PU patch was easier to implant than the ePTFE patch. At 1week after implantation, the SF/PU patch had been infiltrated with cells and collagen fiber. The ePTFE control patch did not accumulate collagen fiber until 3months and calcification occurred at 4weeks. The SF/PU patch did not present any signs of calcification for 3months. This study addressed the problems associated with using SF in isolation and showed that the SF/PU patch can be considered as a useful alternative to the ePTFE to overcome the problem of calcification.
  2017年, 研究論文（学術雑誌）, 共同, 13, 4, 1547-6278, DOI(公開)(r-map), 115, 124
• The effect of beta-caryophyllene on nonalcoholic steatohepatitis
  Arizuka, Naoya; Murakami, Tomoaki; Suzuki, Kazuhiko
  JOURNAL OF TOXICOLOGIC PATHOLOGY
  JAPANESE SOC TOXICOLOGIC PATHOLOGY
  The pathogenesis of nonalcoholic steatohepatitis (NASH) is not fully understood, but many studies have suggested that oxidative stress plays a key role. The methionine-and choline-deficient diet (MCD) administration model can reproduce histopathological features of human NASH and is widely used for investigating NASH. C57BL/6J mice have been used in many studies, but strain differences in pathogenesis have not been sufficiently investigated. We administred MCD to two mouse strains and then compared difference between strains and investigated the effects of beta -caryophyllene (BCP), which possesses an antioxidant effect, on development and progression of NASH. ICR and C57BL/6J mice were administred a control diet, MCD, MCD containing 0.02% BCP, or MCD containing 0.2% BCP. After 4 or 8 weeks, mice were sacrificed. In both strains, MCD administration induced hepatic steatosis and inflammation. These lesions were more severe in C57BL/6J mice than ICR mice, and liver fibrosis was observed at 8 weeks in C57BL/6J mice. These changes were attenuated by BCP coadministration. The mRNA expression of monocyte chemotactic and activating factor (MCP)-1 and fibrosis-related factors increased in C57BL/6J mice, and these increases were reduced by BCP coadministration. The mRNA expression of antioxidant-related factors decreased in both strains, and these decreases were attenuated by BCP coadministration. Based on these results, the C57BL/6J mouse was a more suitable model for MCD-induced NASH than the ICR mouse. In addition, it was suggested that antioxidant effect of BCP might suppressed the damage of hepatocytes caused by oxidative stress and following inflammation and fibrosis.
  2017年, 研究論文（学術雑誌）, 共同, 30, 4, 0914-9198, DOI(公開)(r-map), 263, 273
• Studies on the potential risk of amyloidosis from exposure to silk fibroin
  Tsukawaki S, Murakami T(CA), Suzuki K, Nakazawa Y
  Biomedical Materials
  2016年10月, 研究論文（学術雑誌）, 共同, 11, 6, DOI(公開)(r-map), 065010
• Chicken amyloid arthropathy caused by Mycoplasma synoviae infection in Japan
  Kobayashi N, Murakami T(CA), Sakai H, Yamaguchi Y, Fukushi H, Yanai T
  Journal of Veterinary Science & Medical Diagnosis
  2016年10月, 研究論文（学術雑誌）, 共同, 5, 6, DOI(公開)(r-map)
• Longitudinal study of experimental induction of AA amyloidosis in mice seeded with homologous and heterologous AA fibrils
  Muhammad N, Murakami T, Inoshima Y, Ishiguro N
  Inflammation Research
  2016年09月, 研究論文（学術雑誌）, 共同, 65, 9, DOI(公開)(r-map), 689, 699
• Maternal Exposure to Valproic Acid Primarily Targets Interneurons Followed by Late Effects on Neurogenesis in the Hippocampal Dentate Gyrus in Rat Offspring
  Watanabe Y, Murakami T, Kawashima M, Hasegawa-Baba Y, Mizukami S, Imatanaka N, Akahori Y, Yoshida T, Shibutani M.
  Neurotoxicity Research
  2016年08月, 研究論文（学術雑誌）, 共同, 31, 1, DOI(公開)(r-map), 46, 62
• Spermatogonial Nature of the Germ Cell Component of Canine Testicular Mixed Germ Cell and Stromal Cell Tumours
  Mizukami S, Murakami T, Tanaka T, Machida N, Nomura K, Yoshida T, Shibutani M
  Journal of Comparative Pathology
  2016年07月, 研究論文（学術雑誌）, 共同, 155, 1, DOI(公開)(r-map), 5, 14
• Experimental transmission of systemic AA amyloidosis in autoimmune disease and type 2 diabetes mellitus model mice
  Maeda M, Murakami T, Muhammad N, Inoshima Y, Ishiguro N
  Experimental Animals
  2016年06月, 研究論文（学術雑誌）, 共同, -, -, DOI(公開)(r-map)
• Amyloidosis enhancing activity of bovine amyloid A fibrils in C3H/HeN mice and cynomolgus monkeys (Macaca fascicularis)
  Tsukawaki S, Murakami T(CA), Ibi K, Kuraishi T, Hattori S, Kai C, Suzuki K, Yanai T
  Journal of Medical Primatology
  2016年06月, 研究論文（学術雑誌）, 共同, 45, 3, DOI(公開)(r-map), 112, 117
• Systemic Amyloid A Amyloidosis in a Dog with Sub-acute Time Course after Anesthesia
  Miyake A, Tsukawaki T, Matsuda Y, Kishimoto M, Murakami T and Suzuki K
  J Vet Sci Med Diagn
  2016年02月, 研究論文（学術雑誌）, 共同, 5, 1, DOI(公開)(r-map)
• Effect of heating on the stability of amyloid A (AA) fibrils and the intra- and cross-species transmission of AA amyloidosis
  Ogawa S, Murakami T, Inoshima Y, Ishiguro N
  Amyloid
  2016年01月, 研究論文（学術雑誌）, 共同, 22, 4, DOI(公開)(r-map), 236, 246
• Prevalence of amyloid deposition in mature healthy chickens in the flock that previously had outbreaks of vaccine-associated amyloidosis
  Kanata IBI, Tomoaki MURAKAMI(CA), Wael Mohamed GODA, Naoki KOBAYASHI, Naotaka ISHIGURO and Tokuma YANAI
  The Journal of veterinary medical science
  2015年10月, 研究論文（学術雑誌）, 共同, 70, 10, DOI(公開)(r-map), 1241, 1245
• Systemic AA amyloidosis as a Prion-like disorder
  Murakami T, Inoshima Y, Ishiguro N
  Virus Research
  2015年09月, 共同, 207, -, DOI(公開)(r-map), 76, 81
• Long-term kinetics of AA amyloidosis and effects of inflammatory restimulation after disappearance of amyloid depositions in mice
  Muhammad N, Murakami T, Inoshima Y, Ishiguro N.
  Clinical and experimental immunology
  2015年07月, 研究論文（学術雑誌）, 共同, 181, 1, DOI(公開)(r-map), 133, 141
• Failure of heterogeneous amyloid-enhancing factor in geriatric squirrel monkeys (Saimiri boliviensis)
  Murakami T(CA), Ibi K, Kuraishi T, Hattori S, Kai C, Ishiguro N, Yanai T.
  Journal of Medical Primatology
  2014年12月, 研究論文（学術雑誌）, 共同, 43, 6, DOI(公開)(r-map), 488, 491
• Downregulation of immediate-early genes linking to suppression of neuronal plasticity in rats after 28-day exposure to glycidol
  Akane H, Saito F, Shiraki A, Takeyoshi M, Imatanaka N, Itahashi M, Murakami T, Shibutani M
  Toxicology and applied pharmacology
  2014年06月, 研究論文（学術雑誌）, 共同, 279, 2, DOI(公開)(r-map), 150, 162
• Immunohistochemical characterization of multicentric hepatocholangiocellular adenoma in a pig
  Segawa R, Fujii Y, Ogawa T, Takimoto N, Hara S, Murakami T, Suzuki K, Shibutani M
  Journal of veterinary diagnostic investigation
  2014年05月, 研究論文（学術雑誌）, 共同, 26, 3, DOI(公開)(r-map), 448, 452
• N-Methyl-N-nitrosourea during late gestation results in concomitant but reversible progenitor cell reduction and delayed neurogenesis in the hippocampus of rats
  Itahashi M, Wang L, Shiraki A, Abe H, Tanaka T, Murakami T, Yoshida T, Shibutani M.
  Toxicology letters
  2014年05月, 研究論文（学術雑誌）, 共同, 226, 3, DOI(公開)(r-map), 285, 293
• Transmission of Systemic AA Amyloidosis in Animals
  Murakami T, Ishiguro N, Higuchi K
  Veterinary pathology
  2014年05月, 共同, 51, 2, DOI(公開)(r-map), 363, 371
• Maternal single injection of N-methyl-N-nitrosourea to cause microcephaly in offspring induces transient aberration of hippocampal neurogenesis in mice
  Takimoto N, Wang L, Itahashi M, Ogawa T, Segawa R, Hara S, Murakami T, Suzuki K, Shibutani M
  Toxicology letters
  2014年04月, 研究論文（学術雑誌）, 共同, 226, 1, DOI(公開)(r-map), 20, 27
• ホルスタイン種育成牛にみられた喘鳴症の1症例
  村上智亮, 林口治, 古林与志安, 古岡秀文, 猪熊壽
  北海道獣医師会雑誌
  2014年03月, 研究論文（学術雑誌）, 共同, 58, 3, 85, 87
• Surveillance of Amyloid Deposition and Bacterial Contamination in Chicken Liver from Meat Market
  Naotaka Ishiguro, Tomoaki Murakami, Abdelazim E. Elhelaly and Yasuo Inoshima
  Japan Poultry Science Association
  2014年01月, 研究論文（学術雑誌）, 共同, 51, DOI(公開)(r-map), 104, 107
• An outbreak of lethal adenovirus infection among different otariid species.
  Inoshima Y, Murakami T, Ishiguro N, Hasegawa K, Kasamatsu M
  Veterinary Microbiology
  2013年08月, 研究論文（学術雑誌）, 共同, 165, 3-4, DOI(公開)(r-map), 455, 459
• AA amyloidosis in vaccinated growing chickens.
  Murakami T(CA), Inoshima Y, Sakamoto E, Fukushi H, Sakai H, Yanai T, Ishiguro N.
  Journal of Comparative Pathology
  2013年08月, 研究論文（学術雑誌）, 共同, 149, 2-3, DOI(公開)(r-map), 291, 297
• Experimental induction and oral transmission of avian AA amyloidosis in vaccinated white hens.
  Murakami T(CA), Muhammad N, Inoshima Y, Yanai T, Goryo M, Ishiguro N.
  Amyloid
  2013年06月, 研究論文（学術雑誌）, 共同, 20, 2, DOI(公開)(r-map), 80, 85
• 冷性浮腫のみられなかったアミロイドーシスの乳牛の1症例
  松田浩典, 村上智亮, 鮎川悠, 古林与志安, 猪熊壽
  北海道獣医師会雑誌
  2013年05月, 研究論文（学術雑誌）, 共同, 57, 5, 155, 157
• Pathological Features of Mycobacterium kansasii Infection in Black Bearded Sakis (Chiropotes satanas)
  Murai A, Murakami T, Inoue M, Ueda H, Shiihara S, Kimura J, Hirata A, Sakai H, Yanai T.
  Journal of Comparative Pathology
  2012年11月, 研究論文（学術雑誌）, 共同, 147, 4, DOI(公開)(r-map), 566, 569
• Humeral Chondrosarcoma in a Hokkaido brown bear (Ursus arctos yesoensis)
  Murakami T, Kobayashi Y, Chiba S, Kurauchi Y, Sakamoto H, Sasaki M, Matsui T.
  The Journal of veterinary medical science
  2012年09月, 研究論文（学術雑誌）, 共同, 74, 9, DOI(公開)(r-map), 1195, 1197
• 起立不能を呈したホルスタイン種育成牛にみられた子牛型牛白血病の1症例
  阿部薫、秋長麻衣子、石川温子、石原深雪、井上克徳、下田崇、村上智亮、古林与志安、猪島壽
  北海道獣医師会雑誌
  2012年04月, 研究論文（学術雑誌）, 共同, 56, 4, 128, 130
• Atypical AA amyloid deposits in bovine AA amyloidosis.
  Murakami T, Inoshima Y, Kobayashi Y, Matsui T, Inokuma H, Ishiguro N.
  Amyloid
  2012年03月, 研究論文（学術雑誌）, 共同, 19, 1, DOI(公開)(r-map), 15, 20
• Pathogenesis of experimental amyloid protein A amyloidosis in sore hocks-affected rabbits
  Murakami T, Inoshima Y, Watanabe K, Kobayashi Y, Matsui T, Kurazono H, Ishiguro N.
  Amyloid
  2011年09月, 研究論文（学術雑誌）, 共同, 18, 3, DOI(公開)(r-map), 112, 118
• ホルスタイン乳牛にみられた血様心嚢水貯留の1症例
  松山雄喜, 村上智亮, 下田崇, 古林与志安, 猪熊壽
  北海道獣医師会雑誌
  2011年05月, 研究論文（学術雑誌）, 共同, 55, 5, 176, 178
• ペルシュロン種繁殖雌馬にみられた遠隔転移を伴う腎細胞癌の1症例
  久保田直樹, 村上智亮, 安田峰, 松井基純, 古林与志安, 古岡秀文, 松井高峯, 佐々木直樹, 石井三都夫, 猪熊壽
  北海道獣医師会雑誌
  2011年05月, 研究論文（学術雑誌）, 共同, 55, 5, 10, 12
• 胆道炎を伴う肝線維症の乳牛の1症例
  宇山環, 村上智亮, 坂田貴洋, 山口寿,古林与志安, 古岡秀文, 松井高峯, 石井三都夫, 猪熊壽
  北海道獣医師会雑誌
  2010年07月, 研究論文（学術雑誌）, 共同, 54, 7, 298, 300
• 起立不能を呈した育成牛における非定型散発性牛白血病の1例
  松山雄喜, 村上智亮, 大林哲, 古林与志安, 松本高太郞, 松井高峯, 猪熊壽
  日本獣医師会雑誌
  2010年05月, 研究論文（学術雑誌）, 共同, 63, 5, DOI(公開)(r-map), 355, 358
• 脳幹部膿瘍により神経症状を呈したホルスタイン種子牛の1例
  松本高太郞, 村上智亮, 菅生樹春, 山田一孝, 古林与志安, 松井高峯, 猪熊壽
  日本獣医師会雑誌
  2010年05月, 研究論文（学術雑誌）, 共同, 63, 5, DOI(公開)(r-map), 351, 354
• Isolation and characterization of monoclonal antibodies against bovine serum amyloid A1 protein.
  Taira Y, Inoshima Y, Ishiguro N, Murakami T, Matsui T.
  Amyloid
  2009年12月, 研究論文（学術雑誌）, 共同, 16, 4, DOI(公開)(r-map), 215, 220
• 腹腔内に形成され腫瘍組織の脊柱管内直接浸潤により後躯麻痺を呈した牛白血病の1例
  松山雄喜, 神尾恭平, 村上智亮, 下田崇, 古林与志安, 古岡秀文, 松本高太郞, 猪熊壽.
  日本獣医師会雑誌
  2009年09月, 研究論文（学術雑誌）, 共同, 62, 9, DOI(公開)(r-map), 713, 716
• Imaging diagnosis--porencephaly in a calf
  Lee KJ, Yamada K, Tsuneda R, Kishimoto M, Shimizu J, Murakami T, Kobayashi Y, Furuoka H, Matsui T, Sasaki N, Ishii M, Inokuma H, Miyahara K, Iwasaki T, Miyake Y.
  Veterinary radiology & ultrasound
  2009年05月, 研究論文（学術雑誌）, 共同, 50, 3, DOI(公開)(r-map), 301, 303

著書

• 薬理学・毒性学実験 第4版
  2023年03月01日
• 動物病理学総論 第4版
  2022年03月01日
• 動物病理学各論 第3版
  第2章 脾臓･胸腺, 第4章 気嚢
  文永堂出版
  2021年03月10日

研究発表、招待講演等

• Frequent Occurrence of Fibrinogen Amyloidosis in Japanese Squirrels (Sciurus lis)
  XVIII. Internathinal Symposium on Amyloidosis
  2209年06月, ポスター発表
• Apolipoprotein A-IV amyloidosis in a Cotton-top tamarin (Saguinus oedipus)
  XVIII. Internathinal Symposium on Amyloidosis
  2209年06月, ポスター発表
• モルモットの胃におけるc-kit陰性消化管間質腫瘍の1例
  第9回日本獣医病理学専門家協会学術集会
  2203年30月, ポスター発表
• マイクロミニピッグにおけるAAアミロイドーシスの病因解明の試み
  第9回日本獣医病理学専門家協会学術集会
  2203年30月, ポスター発表
• カスリハタに発生した全身性アミロイド症の1例
  第9回日本獣医病理学専門家協会学術集会
  2203年30月, ポスター発表
• ニホンリス（Sciurus lis）にみられた全身性アミロイドーシスの病因解明
  第9回日本獣医病理学専門家協会学術集
  2203年30月, ポスター発表
• FFPE組織を病態解明に活かすためのプロテオーム解析技術
  第51回日本毒性学会学術年会
  2024年07月04日, 口頭発表（招待・特別）
• 限られた資源から紐解くアミロイドーシス病理の新展開
  日本獣医生命科学大学 獣医学専攻博士課程特別講義
  2024年05月09日, 口頭発表（招待・特別）
• 動物アミロイドーシスの病態解明
  日本大学大学院獣医学研究科サイエンスカフェ2024
  2024年03月11日, 口頭発表（招待・特別）
• OUTBRAKE OF FIBRINOGEN Aα-CHAIN-DERIVED GLOMERULAR AMYLOIDOSIS IN JAPANESE SQUIRRELS
  2023 ACVP/ASVCP Annual Meeting
  2023年10月30日, ポスター発表
• IDENTIFICATION OF LITHOSTATHINE-DERIVED AMYLOID IN FELINE PANCREATIC EXOCRINE TUMORS
  2023 ACVP/ASVCP Annual Meeting
  2023年10月29日, ポスター発表
• CALCITONIN RECEPTOR-STIMULATING PEPTIDE 1-DERIVED AMYLOID DEPOSITION IN A FELINE C-CELL CARCINOMA
  2023 ACVP/ASVCP Annual Meeting
  2023年10月29日, ポスター発表
• IDENTIFICATION OF APOLIPOPROTEIN E-DERIVED AMYLOID WITHIN XANTHOMATOSIS LESIONS OF LEOPARD GECKOS (EUBLEPHARIS MACULARIUS)
  2023 ACVP/ASVCP Annual Meeting
  2023年10月28日, ポスター発表
• SYSTEMIC APOLIPOPROTEIN C-III AMYLOIDOSIS IN A WHITE LION FAMILY
  2023 ACVP/ASVCP Annual Meeting
  2023年10月28日, ポスター発表
• プロテオーム解析を駆使した動物アミロイドーシスの病態解明
  第10回日本アミロイドーシス学会学術集会
  2023年09月23日, 口頭発表（招待・特別）
• ボルネオオランウータン（Pongo pygmaeus）にみられた全身性 ALκ鎖アミロイドーシスの1例
  第10回日本アミロイドーシス学会学術集会
  2023年09月23日, ポスター発表
• 腎臓においてヘテロ接合体に由来する 2種類の AA アミロイドがそれぞれ異なる沈着分布を呈した猫の1例
  第10回日本アミロイドーシス学会学術集会
  2023年09月23日, ポスター発表
• 犬の線維付属器過誤腫に生じた結節性 Apolipoprotein A-I アミロイド沈着
  第10回日本アミロイドーシス学会学術集会
  2023年09月23日, ポスター発表
• 犬の乳腺腫瘍随伴性アミロイドーシスの発生状況の検索およびアミロイド前駆蛋白質の同定
  第10回日本アミロイドーシス学会学術集会
  2023年09月23日, ポスター発表
• Identification of lithostathine-derived amyloid in feline pancreatic exocrine tumors
  第10回日本アミロイドーシス学会学術集会
  2023年09月23日, ポスター発表
• ツシマヤマネコPrionailurus bengalensis euptilurus の胃出血を伴った上皮成長因子含有ファイブリン様細胞外マトリックス蛋白由来アミロイド症の1例
  第10回日本アミロイドーシス学会学術集会
  2023年09月23日, 口頭発表（一般）
• AAアミロイド沈着を伴う犬のセルトリ細胞腫の1例
  第166回日本獣医学会学術集会
  2023年09月05日, 口頭発表（一般）
• 国内に生息するリス属（Sciurus属）におけるアミロイドーシスの病理学的解析
  第166回日本獣医学会学術集会
  2023年09月05日, 口頭発表（一般）
• 大型猫科動物におけるアミロイドーシスの発生状況の調査
  第166回日本獣医学会学術集会
  2023年09月05日, 口頭発表（一般）
• Chicken anemia virus infection in a group of 12-day-old chicks immunized with the maternal antibody
  The 10th ASVP and 10th JCVP Joint Conference
  2023年03月29日, ポスター発表
• Research on the incidence of amyloid deposition in canine mammary tumors
  The 10th ASVP and 10th JCVP Joint Conference
  2023年03月29日, ポスター発表
• Identification of ameloblastin as an amyloid precursor protein of amyloid-producing ameloblastoma in dogs and cats
  The 10th ASVP and 10th JCVP Joint Conference
  2023年03月29日, ポスター発表
• A case of a cat with two different deposition distributions of AA amyloid in the kidney
  The 10th ASVP and 10th JCVP Joint Conference
  2023年03月29日, ポスター発表
• Identification of calcitonin receptor-stimulating peptide 1-derived amyloid in feline C-cell carcinoma
  The 10th ASVP and 10th JCVP Joint Conference
  2023年03月29日, ポスター発表
• 技術紹介：FFPE資料の質量分析に基づく動物アミロイド前駆蛋白質の同定
  第39回日本毒性病理学会学術集会
  2023年01月25日, ポスター発表
• 脳アミロイドβの非標識特異的イメージング手法開発の試み
  第9回日本アミロイドーシス学会学術集会
  2022年10月16日, ポスター発表
• Fibrinogenアミロイドーシスはニホンリスにおいて極めて高頻度に発生する
  第9回日本アミロイドーシス学会学術集会
  2022年10月16日, ポスター発表
• ライオンにみられたApolipoprotein C-IIIアミロイドーシス
  第9回日本アミロイドーシス学会学術集会
  2022年10月16日, ポスター発表
• Identification of systemic ApoA-IV amyloidosis in a cotton-top tamarin (Saguinus oedipus)
  第9回日本アミロイドーシス学会学術集会
  2022年10月16日, ポスター発表
• 犬のα-S1-caseinは過剰発現とN末切断によってアミロイド原性を獲得する
  第9回日本アミロイドーシス学会学術集会
  2022年10月16日, 口頭発表（一般）
• 限局性アミロイドーシスおよび新たに発見された動物アミロイドーシスの病理
  第165回日本獣医学会学術集会病理分科会シンポジウム
  2022年09月06日, 口頭発表（招待・特別）
• 動物アミロイドーシスの病態解明 ~FFPE標本の無限の可能性~
  岐阜大学大学院共同獣医学研究科 学際領域特別演習
  2022年08月31日, 公開講演，セミナー，チュートリアル，講習，講義等
• 構造予測および in vitro 試験に基づく犬乳腺腫瘍随伴アミロイドーシスの病態機序解明
  第9回日本獣医病理学専門家協会学術集会
  2022年03月30日, ポスター発表
• 伝播性AAアミロイドーシスにおけるアミロイド沈着初発部位の探索
  第38回日本毒性病理学会学術集会
  2022年01月26日, ポスター発表
• 動物AAアミロイドーシスの病態多様性と伝播
  第8回日本アミロイドーシス学会学術集会
  2021年11月19日, 口頭発表（招待・特別）
• マウスの局所臓器へのAAアミロイドを直接投与することで誘発されるアミロイド沈着分布および全身移行動態の経時的評価
  第8回日本アミロイドーシス学会学術集会
  2021年11月19日, 口頭発表（一般）
• リスザルの脳アミロイドβの非標識蛍光イメージング
  第8回日本アミロイドーシス学会学術集会
  2021年11月19日, ポスター発表
• 老齢ツシマヤマネコにみられた全身性EFEMP1アミロイドーシス
  第8回日本アミロイドーシス学会学術集会
  2021年11月19日, ポスター発表
• Znrf3エクソン2はマウスX-zoneの分化に重要である：ゲノム編集を用いたin vivo解析
  第54回日本小児内分泌学会学術集会
  2021年10月28日, 口頭発表（一般）
• 犬の毛包腫瘍におけるアミロイド沈着の原因蛋白質同定の試み（続報）
  第164回日本獣医学会学術集会
  2021年09月07日, 口頭発表（一般）
• マウスにおける牛AAアミロイドーシス経口伝播時の「種の壁」に関する研究
  第164回日本獣医学会学術集会
  2021年09月07日, 口頭発表（一般）
• マゼランペンギンの趾瘤を伴った全身性アミロイドAアミロイドーシスの一例
  第8回JCVP学術集会
  2021年03月30日, ポスター発表
• ラット乳腺における新規アミロイドーシスの同定
  第8回JCVP学術集会
  2021年03月30日, 口頭発表（招待・特別）
• 超高齢チンパンジーにおける脳の病理組織学的検索
  第39回日本認知症学会学術集会
  2020年11月26日, ポスター発表
• 犬の毛包腫瘍におけるアミロイド沈着の発生率調査および原因蛋白同定の試み
  第163回日本獣医学会学術集会
  2020年09月14日, 口頭発表（一般）
• 犬の乳腺腫瘍関連アミロイドーシスの原因蛋白質の同定および病態機序解明
  第163回日本獣医学会学術集会
  2020年09月14日, 口頭発表（一般）
• AAアミロイドーシス罹患牛から抽出したアミロイド線維の蛍光指紋解析
  第163回日本獣医学会学術集会
  2020年09月14日, 口頭発表（一般）
• Ex vivo / in vivo試験系を用いたα-シヌクレインフィブリルの経口伝播経路の検証
  第163回日本獣医学会学術集会
  2020年09月14日, 口頭発表（一般）
• ALアミロイドーシス診断におけるレーザーマイクロダイセクション質量分析法の有用性
  第163回日本獣医学会学術集会
  2020年09月14日, 口頭発表（一般）
• キトサン-高分子ミセル複合化ゲルシートの創傷治癒効果 ～ラットの円形切除創を用いた治癒過程評価～
  第69回高分子学会年次大会
  2020年05月27日, ポスター発表
• 豚の黒毛舌の病理学的特徴
  第7回JCVP学術集会
  2020年03月26日, ポスター発表
• 老齢のツシマヤマネコにみられた全身性アミロイドーシス
  第7回JCVP学術集会
  2020年03月26日, ポスター発表
• 超高齢チンパンジーの脳の病理学的検索
  第7回JCVP学術集会
  2020年03月26日, ポスター発表
• イヌの乳腺腫瘍のアミロイド沈着より見出だされたα-S1-カゼインの凝集特性評価
  日本化学会第100春季年会
  2020年03月22日, ポスター発表
• 循環器系組織工学材料を指向したシルクフィブロイン・ポリカーボネート混合不織布の作製と分解性評価
  第41回バイオマテリアル学会
  2019年11月24日, ポスター発表
• 中皮腫様の形態を示した牛の全身性腫瘍の2症例
  関東・東京合同地区獣医三学会公衆衛生学会
  2019年10月27日, 口頭発表（一般）
• 猫アミロイドーシスにおけるアミロイド共存蛋白質に関する研究
  第7回日本アミロイドーシス研究会学術集会
  2019年08月30日, ポスター発表
• レーザーマイクロダイセクション質量分析法を用いた動物ALアミロイドーシスの診断
  第7回日本アミロイドーシス研究会学術集会
  2019年08月30日, ポスター発表
• イヌの乳腺腫瘍随伴アミロイドーシスにおける前駆タンパク同定の試み（続報）
  第7回日本アミロイドーシス研究会学術集会
  2019年08月30日, ポスター発表
• 家禽のアミロイドーシス
  鶏病研究会栃木県支部第1回研修会
  2019年07月16日, 口頭発表（招待・特別）
• 採卵鶏の腹膜に多発した水胞病変1例についての鑑別および診断
  平成30年度食肉及び食鳥肉衛生研究発表会
  2019年01月21日, 口頭発表（一般）
• 鶏の腹腔内多発性水胞
  全国食肉衛生検査所協議会病理部会研修会
  2018年11月15日, 口頭発表（一般）
• ラット乳腺アミロイドーシスにおける新規アミロイドタンパクの同定
  第161回日本獣医学会学術集会
  2018年09月12日, 口頭発表（一般）
• AAアミロイドーシス罹患フラミンゴにおける脳血管アミロイドーシス
  第161回日本獣医学会学術集会
  2018年09月12日, 口頭発表（一般）
• 公衆衛生領域におけるアミロイドーシス研究の意義
  平成30年度日本獣医公衆衛生学会（中部）
  2018年09月02日, 口頭発表（招待・特別）
• 動物アミロイドーシスの病態と伝播
  第6回日本アミロイドーシス研究会学術集会
  2018年08月25日, 口頭発表（招待・特別）
• AAアミロイドーシス罹患フラミンゴにおける脳血管アミロイド症
  第6回日本アミロイドーシス研究会学術集会
  2018年08月25日, ポスター発表
• 若齢ウズラにおけるAAアミロイド症の経口伝播
  第6回日本アミロイドーシス研究会学術集会
  2018年08月25日, ポスター発表
• イヌの乳腺腫瘍随伴アミロイドーシスにおける前駆タンパク同定の試み
  第6回日本アミロイドーシス研究会学術集会
  2018年08月25日, ポスター発表
• Pathology and Transmission of Amyloidosis in Animals
  第10回バイオメディカル研究所国際シンポジウム
  2018年08月24日, 口頭発表（招待・特別）
• Localized Amyloidosis in Rat Mammary Gland
  The XVIth International Symposium on Amyloidosis
  2018年03月26日, ポスター発表
• シスプラチンの腎毒性に対するエダラボンの効果
  第34回日本毒性病理学会学術集会
  2018年01月25日, ポスター発表
• フマル酸ジメチルの腎間質線維化抑制効果の検討
  第34回日本毒性病理学会学術集会
  2018年01月25日, ポスター発表
• HNF4α発現低下に伴う尿細管上皮細胞の形質変化
  第34回日本毒性病理学会学術集会
  2018年01月25日, ポスター発表
• Molecular Characterization and Isolation of MuBV-1 from Bengalese finch (Lonchura striata f. domestica) in Japan
  第41回日本鳥類内分泌研究会
  2017年12月17日, 口頭発表（一般）
• ニホンウズラにおけるAAアミロイド症の病態および伝播性
  第41回日本鳥類内分泌研究会
  2017年12月16日, 口頭発表（一般）
• ウズラAAアミロイド症の水平伝播性に関する研究
  第160回日本獣医学会学術集会
  2017年09月13日, 口頭発表（一般）
• ウズラAAアミロイド症の実験的経口伝播
  第5回日本アミロイドーシス研究会学術集会
  2017年08月19日, ポスター発表
• ラットの乳腺癌にみられた新規アミロイド症の同定の試み
  第5回日本アミロイドーシス研究会学術集会
  2017年08月19日, ポスター発表
• 組織工学材料を指向したB. moriシルクフィブロインの生体適合性研究
  第38回日本炎症･再生医学会
  2017年07月18日, ポスター発表
• ALV感染鶏の腸管にみられた悪性中皮腫および骨髄球腫の病理学的研究
  第4回日本獣医病理学専門家協会学術集会
  2017年03月30日, ポスター発表
• Tyrを介したシルクフィブロイン化学修飾による組織工学材料の構築
  第16回日本再生医療学会総会
  2017年03月08日, 口頭発表（一般）
• 組織工学材料を指向したシルクフィブロイン/ポリウレタン複合化材料の創製
  第16回日本再生医療学会総会
  2017年03月08日, 口頭発表（一般）
• ラットの乳腺腫瘍に認められた限局性アミロイド症の1例
  第33回2本毒性病理学会総会及び学術集会
  2017年01月26日, 口頭発表（一般）
• 非アルコール性脂肪性肝炎およびその発がん進展におけるβ-caryophylleneの影響
  第159回日本獣医学会学術集会
  2016年09月06日, 口頭発表（一般）
• 抗酸化物質摂取によるAAアミロイド症抑制効果の検討
  第159回日本獣医学会学術集会
  2016年09月06日, 口頭発表（一般）
• 鳥類AAアミロイド症の病態および水平伝播性に関する基礎研究
  第159回日本獣医学会学術集会
  2016年09月06日, 口頭発表（一般）
• A FOLLOW UP STUDY OF AA AMYLOIDOSIS IN AGED HENS WITH AN OUTBREAK WHILE IN JUVENILE
  The 19th Federation of Asian Veterinary Associations Congress
  2016年09月06日, 口頭発表（一般）
• シルクフィブロイン－合成高分子複合化による心臓修復パッチへの応用
  第37回日本炎症･再生医学会
  2016年06月17日, ポスター発表
• 心臓修復パッチを指向したシルクフィブロイン/ポリウレタン複合化材料の開発
  平成28年度繊維学会年次大会
  2016年06月10日, ポスター発表
• シルクフィブロイン /合成高分子複膜の作製と心臓修復パッチへ応用
  平成28年度繊維学会年次大会
  2016年06月08日, ポスター発表
• 心臓修復パッチへの応用に向けたシルクフィブロイン-合成高分子複合膜の作製と評価
  第65回高分子学会年次大会
  2016年05月27日, ポスター発表
• 組織再生型心臓弁を指向したエラスチン架橋型シルクフィブロインの作製と評価
  第65回高分子学会年次大会
  2016年05月25日, ポスター発表
• シルクフィブロイン暴露に伴うAAアミロイド症発症リスクに関する研究
  第158回日本獣医学会学術集会
  2015年09月08日, 口頭発表（一般）
• 肢関節の腫大を示した褐色採卵鶏におけるアミロイド関節症の病理学的特徴
  第158回日本獣医学会学術集会
  2015年09月07日, 口頭発表（一般）
• ワクチン関連ニワトリAAアミロイド症に関する研究
  第3回日本アミロイドーシス研究会学術集会
  2015年08月21日, 口頭発表（一般）
• 育成鶏におけるワクチン関連AAアミロイド症：病理学的特徴及び誘発実験
  平成26年度日本獣医師会獣医学述学会年次大会
  2015年02月14日, 口頭発表（一般）
• ワクチン関連の鶏アミロイド症
  第21回岐山毒性病理セミナー
  2014年10月25日, 口頭発表（招待・特別）
• 28日間反復投与毒性試験の枠組みでのマンガン投与による海馬歯状回ニューロン新生傷害の検討
  第157回日本獣医学会学術集会
  2014年09月10日, その他
• 脱髄誘発物質ヘキサクロロフェンのマウスへの発達期暴露による離乳時の海馬ニューロン新生に対する影響
  第157回日本獣医学会学術集会
  2014年09月10日, その他
• 6-プロピル-2-チオウラシル（PTU）のラット発達期暴露及び28日間反復投与による甲状腺機能低下を介したニューロン・グリア発達障害に関連する指標分子の検索
  第157回日本獣医学会学術集会
  2014年09月10日, その他
• Thioacetamide のラットへの28日間投与により肝臓で過メチル化し発現が下方制御される分子の肝細胞前がん病変における発現挙動
  第157回日本獣医学会学術集会
  2014年09月10日, その他
• Long-term study of amyloid transmission within and across the animal species in murine AA amyloidosis model
  第157回日本獣医学会学術集会
  2014年09月09日, その他
• 加熱処理がAAアミロイドの伝播性に与える影響
  第157回日本獣医学会学術集会
  2014年09月09日, その他
• カニクイザルとマウスを用いたウシAAアミロイド症の伝達実験
  第157回日本獣医学会学術集会
  2014年09月09日, その他
• AAアミロイド症を耐過した成熟鶏群におけるアミロイド沈着量の変化
  第157回日本獣医学会学術集会
  2014年09月09日, その他
• リスザルおよびカニクイザルを用いたウシAAアミロイド症伝達実験
  第2回日本アミロイドーシス研究会学術集会
  2014年08月22日, 口頭発表（一般）
• 私の背景の紹介と今後の展望 -動物の伝達性アミロイド症-
  東京農工大学 同窓会獣医学部会研究講演会
  2014年07月05日, 口頭発表（招待・特別）
• 髄誘発物質ヘキサクロロフェンの発達期暴露によるラット海馬歯状回における離乳時ニューロン新生に対する影響
  第41回日本毒性学会学術年会
  2014年07月02日, ポスター発表
• T-2トキシンの発達期暴露によるラット海馬歯状回における離乳時ニューロン新生に対する影響
  第41回日本毒性学会学術年会
  2014年07月02日, ポスター発表
• 髄誘発物質クプリゾンのラットへの発達期暴露による離乳時の海馬ニューロン新生に対する影響
  第41回日本毒性学会学術年会
  2014年07月02日, ポスター発表
• Transmission and regression of vaccine-associated chicken AA amyloidosis.
  XIVth Symposium on Amyloidosis
  2014年04月28日, ポスター発表
• Long-term study of AA amyloidosis mechanism within and across the animal species.
  XIVth Symposium on Amyloidosis
  2014年04月28日, ポスター発表
• 動物の伝達性アミロイド症（Transmissible animal amyloidosis）
  東京農工大学 大学院セミナー
  2014年02月27日, 口頭発表（招待・特別）
• AN OUTBREAK OF AA AMYLOIDOSIS IN A GROWING HEN FARM IN JAPAN
  Myanmar Veterinary Association Conference
  2014年02月02日, その他
• Omeprazole の混餌投与によるラット肝発がんプロモーション作用閾値の検索
  第30回日本毒性病理学会総会及び学術集会
  2014年01月30日, ポスター発表
• ラットの発がん物質投与初期に誘発される増殖活性亢進時における細胞周期制御異常の特異性に関する検討
  第30回日本毒性病理学会総会及び学術集会
  2014年01月30日, ポスター発表
• Azoxymethane 投与により発生したラットの十二指腸腫瘍の由来細胞の検討
  第30回日本毒性病理学会総会及び学術集会
  2014年01月30日, ポスター発表
• イミノジプロピオニトリルのラットへの発達期暴露による海馬歯状回における離乳時ニューロン新生に対する影響
  第30回日本毒性病理学会総会及び学術集会
  2014年01月30日, ポスター発表
• 6-propyl-2-thiouracil （PTU）のラット 28日間反復投与による甲状腺機能低下を介したニューロン・グリア発達障害の検出
  第30回日本毒性病理学会総会及び学術集会
  2014年01月30日, ポスター発表
• イミノジプロピオニトリル発達期暴露によるマウス海馬歯状回の神経発生に及ぼす影響
  第30回日本毒性病理学会総会及び学術集会
  2014年01月30日, ポスター発表

委員歴

• 獣医学教育支援機構
  vetCBT委員会 問題精選分科会委員
  自 202304, 至 202503
• 獣医事審議会
  専門委員
  自 202304, 至 202503
• 日本アミロイドーシス学会
  理事
  自 202211, 至 202403
• 日本獣医病理学専門家協会
  評議員
  自 20210910, 至 202403

メディア報道

• 科学スコープ　飼育動物に老年病が増加？　野生では見られぬ変化も　異常タンパク蓄積確認
  山陰中央新報デジタル
  自 2025年01月06日, 至 2025年01月06日
• 異常タンパク蓄積 ライオン、ニホンリスで確認
  山梨日日新聞デジタル
  自 2024年12月16日, 至 2024年12月16日
• 飼育動物、長寿化による病気増加
  神戸新聞
  自 2024年12月12日, 至 2024年12月12日
• ホワイトライオンから人の超希少疾患　農工大准教授らが確認
  文教速報
  自 2024年02月16日, 至 2024年02月16日
• Japanese squirrels develop human hereditary diseases with ageing
  Researchers at Tokyo University of Agriculture and Technology, led by Dr Tomoaki Murakami, have uncovered a previously undocumented disease, fibrinogen Aα-chain amyloidosis, highly prevalent in Japanese squirrels, shedding light on its parallels with the human condition.
  Drug TARGET REVIEW
  自 2023年09月14日, 至 2023年09月14日
• Japanese squirrels develop human hereditary diseases with aging
  EurekAlert!
  自 2023年08月18日, 至 2023年08月18日
• 東京農工大学がフィブリノゲンα鎖アミロイドーシスを人以外で初めて発見
  大学ジャーナルONLINE/NewsPicks10
  自 2023年08月18日, 至 2023年08月18日
• Japanese squirrels found to develop human hereditary diseases with aging
  A research group led by Dr. Tomoaki Murakami from the Tokyo University of Agriculture and Technology has revealed that fibrinogen Aα-chain amyloidosis, a previously unreported disease in animals other than humans, is highly prevalent in Japanese squirrels (Sciurus lis). In this study, they compared the pathology with that of humans, and suggested the importance of Japanese squirrels in the comparative pathological analysis of fibrinogen Aα-chain amyloidosis.
  PHYS ORG
  自 2023年08月18日, 至 2023年08月18日
• 東京農工大など、フィブリノゲンα鎖アミロイドーシスを人以外で発見
  日本経済新聞
  自 2023年08月09日, 至 2023年08月09日
• Novel amyloid protein discovered from canine mammary tumors
  News Medical Life Sciences
  自 2023年01月24日, 至 2023年01月24日
• Researchers uncover novel amyloidosis　α-S1-casein forms amyloid by overexpression and N-terminal truncation in canine mammary tumor
  A collaboration led by scientists at Tokyo University of Agriculture and Technology (TUAT), Japan, has discovered a novel amyloid protein from canine mammary tumors.
  EurekAlert!／Phys.Org／News-Medical.Net
  自 2023年01月23日, 至 2023年01月24日
• 農工大，自家蛍光でアミロイドの迅速検出に成功
  OPTRONICS ONLINE
  自 2021年10月07日, 至 2021年10月07日
• 農工大、牛の致死性疾患「AAアミロイドーシス」の迅速診断技術を開発
  東京農工大学（村上智亮准教授、氏家直毅大学院生、岩出進大学院生）とコニカミノルタの共同研究チームが、「アミロイドA」(AA)が全身に沈着することで引き起こされる牛の致死性疾患「AAアミロイドーシス」を迅速に診断するための技術を開発したことが紹介される。
  マイナビニュース／ニコニコニュース／gooニュース
  自 2021年10月07日, 至 2021年10月07日
• Fast and easy detection of amyloid through a fluorescence fingerprinting approach
  東京農工大学（村上智亮准教授、氏家直毅大学院生、岩出進大学院生）とコニカミノルタの共同研究チームが、「アミロイドA」(AA)が全身に沈着することで引き起こされる牛の致死性疾患「AAアミロイドーシス」を迅速に診断するための技術を開発したことが紹介される。
  PHYS ORG
  自 2021年10月07日, 至 2021年10月07日
• 東京農工大など、自家蛍光を利用したアミロイドの迅速検出法を開発
  日本経済新聞
  自 2021年10月06日, 至 2021年10月06日
• 異常アミロイドの原因特定
  ラット乳腺の新規アミロイド発見に関する報道
  日刊工業新聞
  自 2019年11月14日, 至 2019年11月14日
• Novel amyloid protein that induces amyloidosis discovered
  A collaboration led by scientists at Tokyo University of Agriculture and Technology (TUAT), Japan, has discovered a novel amyloid protein that induces amyloidosis in rats.
  NEWS Medical Life Science
  自 2019年10月25日, 至 2019年10月25日

所属学協会

• International Society of Amyloidosis
  自 2019年10月07日
• 日本獣医病理学専門家協会（JCVP）
• 日本獣医学会
• 日本アミロイドーシス学会
• 日本毒性病理学会

受賞

• 日本獣医病理学専門家協会
  第10回日本獣医病理学専門家協会学術集会 優秀ポスター賞
  猫の C 細胞癌におけるカルシトニン受容体刺激ペプチド１由来アミロイドの同定
  日本獣医病理学専門家協会主催 第10回学術集会のポスター発表において、優秀な発表を行った者に贈られる。
  2023年03月31日
• 日本獣医病理学専門家協会
  JCVP奨励賞
  ラット乳腺における新規アミロイドーシスの同定
  2021年03月30日
• 東京農工大学
  学長賞
  農工融合論文発表部門
  2019年10月29日
• 東京農工大学
  学長賞
  農工融合論文発表部門
  2018年10月16日