研究者データベース

倉科 佑太KURASHINA Yutaクラシナ ユウタ

所属部署名工学研究院 先端機械システム部門
職名准教授
Last Updated :2023/09/14

業績情報

氏名・連絡先

  • 氏名

    クラシナ ユウタ, 倉科 佑太, KURASHINA Yuta

主たる所属・職名

  • 工学研究院 先端機械システム部門, 准教授

その他の所属

  • 工学部 機械システム工学科

科学研究費助成事業

  • 基盤研究(B)
    重畳化した超音波照射による生体高分子の経皮薬剤送達システムの構築
    自 2021年, 至 2023年
  • 挑戦的研究(萌芽)
    超音波を用いたポリフェノール配合マイクロニードル薬剤投与の高速化
    自 2022年, 至 2023年
  • 国際共同研究加速基金(国際共同研究強化(B))
    表面弾性波を用いたin situ波形測定による細胞組織の成熟評価AIシステムの構築
    自 2022年, 至 2025年
  • 基盤研究(B)
    分散データリザレクション:柔軟性と効率性を兼ね備えた計測情報再構成技術の創出
    自 2022年, 至 2022年
  • 基盤研究(B)
    がん診断治療への最適化を目指したマルチコア構造磁性ナノ粒子の磁化応答モデル構築
    自 2022年, 至 2022年
  • 挑戦的研究(萌芽)
    がん転移に関わる細胞分化機構解明を目的とした細胞-細胞間引張強度測定技術の創成
    自 2022年, 至 2022年

論文

  • A novel fabrication process of up-scalable microfiber-shaped tendon-like tissue with high cell density for uniformed macroscale assembly
    Fukada, Keisuke; Tachibana, Koji; Kurashina, Yuta; Kaneko, Yosuke; Matsumoto, Tatsuaki; Miyamoto, Takeshi; Niki, Yasuo; Nakamura, Masaya; Onoe, Hiroaki
    BIOTECHNOLOGY AND BIOENGINEERING
    WILEY
    This paper describes up-scalable microfiber-shaped tissues for macroscale tendon tissue reconstruction in vitro. C3H10T1/2 cells were encapsulated in a calcium alginate hydrogel microfiber that was fabricated via a double coaxial microfluidic device. The C3H10T1/2 cells gradually merged to construct the microfiber-shaped tendon-like tissue. Our microfiber-shaped tendon-like tissues were alive and maintained their microfiber-shaped morphology over 600 days. Immunostaining and real-time quantitative polymerase chain reaction analyses showed that our fabricated microfiber-shaped tendon-like tissue properly expressed tenomodulin and the orientation of the filaments of actin, which are one of the characteristics of tendon tissue in vivo. Furthermore, a macroscale tendon tissue assembly with similar to 1 cm in length and similar to 200 mu m in thickness was successfully constructed by bundling the microfiber-shaped tendon-like tissues together. This feature enabled us to fabricate a macroscale tendon tissue with uniform cell distribution. We believe that our fabricated microfiber-shaped tendon-like tissue would be a suitable strategy to reconstruct tendon tissue in vitro for the treatments of tendon-related injuries.
    2022年05月, 研究論文(学術雑誌), 共同, 119, 5, 0006-3592, DOI(公開)(r-map), 1327, 1336
  • Transdermal delivery of bFGF with sonophoresis facilitated by chitosan nanocarriers
    Xie, Xue; Kurashina, Yuta; Matsui, Makoto; Nomoto, Takahiro; Itoh, Munenari; Okano, Hirotaka J.; Nakamura, Kentaro; Nishiyama, Nobuhiro; Kitamoto, Yoshitaka
    JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
    ELSEVIER
    The basic fibroblast growth factor (bFGF) is a potent angiogenic growth factor. Supplemental bFGF is essential for healing damaged skin, but its therapeutic use has been limited owing to its inherent instability. In this study, we propose a transdermal delivery system using sonophoresis along with the use of carboxymethyl chitosan nanocarriers which enables the administration of bFGF over a wide area of skin. The transdermal effect of the low-frequency ultrasound-enhanced delivery system was demonstrated in ex vivo experiments using hairless mouse skin. The subcutaneous administration of chitosan nanoparticles was evaluated by using fluorescencelabeled chitosan. To stably load bFGF into chitosan nanoparticles, a carrier with carboxymethyl chitosan was synthesized under a pH of around 7. Based on the results, we evaluated the effectiveness of transdermal drug delivery using sonophoresis. In skin cryosections, bFGF-loaded carboxymethyl chitosan nanocarriers delivered by ultrasound irradiation were present subcutaneously. We analyzed the bFGF content in skin tissue by ELISA and found that bFGF penetration in skin irradiated by ultrasound with nanocarriers. Penetration of bFGF-leaded carboxymethyl chitosan nanocarriers by ultrasound was significantly higher than that by ultrasound irradiation alone. Our results lay the groundwork for further utilization of polymeric nanocarriers using sonophoresis for the administration of biomacromolecules.
    2022年09月, 研究論文(学術雑誌), 共同, 75, 1773-2247, DOI(公開)(r-map)
  • Vascular embolization of radiopaque hydrogel microfiber using ultra-minimally invasive technique for stage-adjustable renal failure model
    Takakura, Naoki; Ohta, Hiroki; Komatsu, Teppei; Kurashina, Yuta; Hiroka, Yuya; Okano, Hirotaka J.; Onoe, Hiroaki
    MATERIALS & DESIGN
    ELSEVIER SCI LTD
    Animal pathology models play an important role in elucidating pathology, evaluating drug efficacy, and studying therapeutic. This paper presents the creation of rats' renal failure model by embolizing the renal artery using radiopaque hydrogel microfibers. By using a dual coaxial laminar flow microfluidic device, barium alginate gel microfibers containing zirconia particles were fabricated. Since the zirconia particles are radiopaque, the microfiber can be delivered and embolized while confirming the position of the microfiber in real-time under X-ray imaging. The delivery of microfibers through a catheter into the renal artery of rats to create a renal failure model was tested. The radiopaque microfibers were visualized by X-ray imaging when delivered by a catheter. Furthermore, the microfibers remained stable for 2 weeks after delivery. In addition, by adjusting the number of microfibers, two different rat models with severe and mild renal failure conditions were created. The proposed delivery of radiopaque hydrogel microfibers to create an embolization model is expected to be an effective approach to control the degree of symptoms and elucidate the pathology. (c) 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
    2022年07月, 研究論文(学術雑誌), 共同, 219, 0264-1275, DOI(公開)(r-map)
  • QUANTITATIVE ANALYSIS OF ACOUSTIC PRESSURE FOR SONOPHORESIS AND ITS EFFECT ON TRANSDERMAL PENETRATION
    Kurashina, Yuta; Asano, Risa; Matsui, Makoto; Nomoto, Takahiro; Ando, Keita; Nakamura, Kentaro; Nishiyama, Nobuhiro; Kitamoto, Yoshitaka
    ULTRASOUND IN MEDICINE AND BIOLOGY
    ELSEVIER SCIENCE INC
    Ultrasound facilitates the penetration of macromolecular compounds through the skin and offers a promising non-invasive technique for transdermal delivery. However, technical difficulties in quantifying ultrasound-related parameters have restricted further analysis of the sonophoresis mechanism. In this study, we devise a bolt-clamped Langevin transducer-based sonophoresis device that enables us to measure with a thin lead zirconate titanate (PZT) sensor. One-dimensional acoustic theory accounting for wave interaction at the skin interface indicates that the acoustic pressure and cavitation onset on the skin during sonophoresis are sensitive to the subcutaneous support, meaning that there is a strong need to perform the pressure measurement in an experimental environment replacing the human body. From a series of the experiments with our new device, the transdermal penetration of polystyrene, silica and gold nanoparticles is found to depend on the size and material of the particles, as well as the hardness of the subcutaneous support material. We speculate from the acoustic pressure measurement that the particles' penetration results from the mechanical action of cavitation.(E-mail: kurashina@mac.titech.ac.jp) (c) 2022 World Federation for Ultrasound in Medicine & Biology. All rights reserved.
    2022年05月, 研究論文(学術雑誌), 共同, 48, 5, 0301-5629, DOI(公開)(r-map), 933, 944
  • Effect of the area of a lithium niobate transducer on the efficiency of ultrasonic atomization driven by resonance vibration
    Yoshioka, Keisuke; Kurashina, Yuta; Ogawa, Ami; Asakura, Takumi
    ULTRASONICS SONOCHEMISTRY
    ELSEVIER
    In recent years, individual control of one's personal environment has been drawing increasing attention due to the growing interest in health care. Wearable devices are especially useful because of their controllability regardless of location. Humidity is one of the inevitable factors in the personal environment as a preventive against infectious diseases. Although atomization devices are commonly used as a method of humidity control, at present, there are no wearable humidity control devices. Vibration of a lithium niobate (LN) device in the thickness mode is a promising piezoelectric method for miniaturization of atomization devices for humidity control. To miniaturize the atomization device, the transducer size needs to be small not so much as to decrease the atomization efficiency. However, the effect of the device area on the atomization efficiency of LN at a size suitable for mounting in wearable devices has not been studied. Here, we conducted an atomization demon-stration of LN devices with different sizes to evaluate particle size and atomization efficiency. Furthermore, to reveal the relationship between vibration behavior and atomization efficiency, resonance vibration in the MHz frequency band was evaluated by the finite element method and an impedance analyzer. The results showed that the peak size of water particles atomized by each device was in the range of 3.2 to 4.2 mu m, which is smaller than particles produced by typical piezoelectric ceramics. Moreover, the best LN size for efficient atomization was found to be 8 mm x 10 mm among the five LN device sizes used in experiments. From the relationship between vibration behavior and atomization efficiency, the size of the transducer was suggested to affect the vibration mode. The obtained result suggested that the LN device is suitable for small wearable nebulizer devices.
    2022年05月, 研究論文(学術雑誌), 共同, 86, 1350-4177, DOI(公開)(r-map)
  • Combined analysis of finite element model and audiometry provides insights into the pathogenesis of conductive hearing loss
    Hirabayashi, Motoki; Kurihara, Sho; Ito, Ryuya; Kurashina, Yuta; Motegi, Masaomi; Okano, Hirotaka James; Yamamoto, Yutaka; Kojima, Hiromi; Asakura, Takumi
    FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
    FRONTIERS MEDIA SA
    The middle ear transmits sound to the inner ear via vibrations in the eardrum and ossicles, and damage to the middle ear results in conductive hearing loss. Although conductive hearing loss can be corrected by surgery, the currently available clinical investigations cannot always diagnose the ossicular chain pathology underlying the conductive hearing loss, and even intraoperative findings can be equivocal. Acoustic analysis using finite element models (FEMs) can simulate the sound pressure change at an arbitrary site for each frequency. FEMs are used in acoustic engineering to simulate the frequency-dependent sound pressure distribution at discrete cells in a simulated model and analyze the effects of specific parameters on the audiogram. However, few reports have compared the numerical results obtained using FEMs with data from clinical cases. We used FEMs to simulate audiograms of the air-bone gap (ABG) for various ossicular chain defects and compared these with preoperative audiograms obtained from 44 patients with a normal tympanic membrane who had otosclerosis, middle ear malformations or traumatic ossicular disruption. The simulated audiograms for otosclerosis and attic fixation of the malleus-incus complex both exhibited an up-slope but could be distinguished from each other based on the ABG at 1000 Hz. The simulated audiogram for incudostapedial joint discontinuity exhibited a peak at around 750 Hz and a down-slope above 1000 Hz. In general, the simulated audiograms for otosclerosis, attic fixation and incudostapedial joint discontinuity were consistent with those obtained from clinical cases. Additional simulations indicated that changes in ossicular mass had relatively small effects on ABG. Furthermore, analyses of combination pathologies suggested that the effects of one defect on ABG were added to those of the other defect. These FEM-based findings provide insights into the pathogenesis of conductive hearing loss due to otosclerosis, middle ear malformations and traumatic injury.
    2022年09月02日, 研究論文(学術雑誌), 共同, 10, 2296-4185, DOI(公開)(r-map)
  • Near-Infrared-Triggered On-Demand Controlled Release of Adeno-Associated Virus from Alginate Hydrogel Microbeads with Heat Transducer for Gene Therapy
    Takatsuka, Shuhei; Kubota, Takeshi; Kurashina, Yuta; Onoe, Hiroaki
    SMALL
    WILEY
    2023年02月, 研究論文(学術雑誌), 共同, 19, 7, 1613-6810, DOI(公開)(r-map)
  • Synthesis and characterization of pH-responsive ferrogels comprising sulfamethazine-based polymer and magnetic nanoparticles for sensing ammonia gas
    JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
    ELSEVIER
    2023年01月, 研究論文(学術雑誌), 共同, 565, 170201, 0304-8853, DOI(公開)(r-map)

委員歴

  • 日本機械学会 機械力学・計測制御分野
    若手交流実行委員長
    自 202204, 至 202208
  • International Workshop on Piezoelectric Materials and Applications in Actuators
    実行委員
    自 202204, 至 202210


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