寺田　昭彦TERADA Akihikoテラダ　アキヒコ

業績情報

氏名・連絡先

• 氏名

  テラダ　アキヒコ, 寺田　昭彦, TERADA Akihiko

• eメールアドレス

  aktecc.tuat.ac.jp

• 個人ホームページ

  http://web.tuat.ac.jp/~tuatebel/

主たる所属・職名

• 工学研究院　応用化学部門, 教授

その他の所属

• 工学府　産業技術専攻
• 工学府　応用化学専攻
• 工学部　化学物理工学科
• グローバルイノベーション研究院

経歴

• -
  東京農工大学大学院工学研究院　准教授
  自 2012年04月01日, 至 2017年11月30日
• -
  東京農工大学大学院共生科学技術研究院　講師
  自 2009年04月01日, 至 2012年03月31日
• -
  デンマーク工科大学環境工学科　博士研究員
  自 2006年08月01日, 至 2009年03月31日
• -
  早稲田大学理工学総合研究センター　助手
  自 2006年04月01日, 至 2006年07月31日
• -
  デンマーク工科大学環境資源研究所　客員研究員
  自 2005年04月01日, 至 2005年11月15日

学歴

• 早稲田大学大学院
  理工学研究科
  応用化学専攻
  至 2006年03月, 修了, 博士後期
• 早稲田大学大学院
  理工学研究科
  応用化学専攻
  至 2003年03月, 修了, 博士前期

免許・資格

• 公害防止管理者（水質関係第1）
  2003年11月

教育・研究活動状況

• 地球環境に存在する様々な微生物の生き様を正しく理解し、環境浄化にとって有用な種類の探索・獲得を行います。獲得された有用微生物を制御することにより、省エネ型水処理、排水中に含まれる資源回収、温室効果ガス排出削減に向けた技術開発を行います。

研究分野

• A389　環境・農学, A64020　環境負荷低減技術、保全修復技術
• A789　社会基盤（土木・建築・防災）, A22060　土木環境システム
• A689　ものづくり技術（機械・電気電子・化学工学）, A27040　バイオ機能応用、バイオプロセス工学
• A189　ライフサイエンス, A43010　分子生物学
• A189　ライフサイエンス, A45030　多様性生物学、分類学

研究キーワード

• 自然環境中に雑多に存在する微生物を有効活用して水環境の浄化および有用物質の生産を目指す．具体的には，難生分解性有機化合物・窒素除去を志向したバイオリアクターシステムの開発，畜産排水除去の性能向上を目指した水田土壌における微生物叢の解析・制御など．さらには、微生物の集合体であるバイオフィルムコントロールを目指した材料表面の開発およびバイオフィルム形成防止のための戦術の開発

研究テーマ

• 1. Microbial Resource Management (MRM)に基づく複合微生物系のバイオプロセスへの利用
  自 200904
• 2. ガス透過膜に形成させたバイオフィルムを用いた難生分解性有機化合物の単一槽内酸化・還元
  自 200904
• 3. バイオフィルム制御へ向けた固定化材料表面の修飾技術の確立（Surface Modification for Biofilm Control (SuMoBiC)）
  自 200904
• 4. 微生物叢制御による低コスト・省エネルギー型排水処理システムの開発
  自 201004, 至 201503

担当授業科目

• Environmental Engineering and Microbiology(3学期・金3,4・工学部)
  2023年, 専門科目等
• Research Internship(3学期・集中・工学部)
  2023年, 専門科目等
• インターンシップⅠ(通年・集中・工学府博士前期)
  2023年, 専門科目等
• 化学物理工学先端プロジェクト(3学期・木3・工学部)
  2023年, 専門科目等
• 環境技術プロジェクトマネジメント(3学期・火7・工学府専門職学位)
  2023年, 専門科目等
• 環境工学特論Ⅰ(3学期・金3・工学府博士前期)
  2023年, 専門科目等
• 産業技術実践研究Ⅱ(通年・集中・工学府専門職学位)
  2023年, 専門科目等
• 生物化学(3学期・木4・工学部)
  2023年, 専門科目等
• 生物学基礎(3学期・火1・工学部)
  2023年, 専門科目等
• 農工協働科目(集中・集中・農学部)
  2023年, 教養科目等

科学研究費助成事業

• 基盤研究（B）
  生成ポテンシャルの評価を基軸とした亜酸化窒素の突発的発生現象の機構解明
  自 2023年, 至 2023年
• 基盤研究(B)
  高活性N2O還元型リン蓄積細菌を用いた革新的温室効果ガス削減・リン回収技術への展開
  自 2023年, 至 2025年
• 基盤研究(B)
  生成ポテンシャルの評価を基軸とした亜酸化窒素の突発的発生現象の機構解明
  自 2022年, 至 2022年
• 基盤研究(A)
  多重同位体標識窒素化合物（MILNC）による超高精度窒素循環解析
  自 2021年, 至 2021年
• 基盤研究(B)
  生成ポテンシャルの評価を基軸とした亜酸化窒素の突発的発生現象の機構解明
  自 2021年, 至 2021年
• 基盤研究(B)
  新規培養法と安定同位体プローブ法で迫るN2O還元細菌の新機能：排出削減と物質産生
  自 2020年, 至 2022年
• 国際共同研究加速基金（国際共同研究強化(B)）
  高効率窒素除去プロセスの温室効果ガス削減に向けた亜酸化窒素還元細菌の全容解明
  自 2020年, 至 2023年
• 基盤研究(A)
  多重同位体標識窒素化合物（MILNC）による超高精度窒素循環解析
  自 2020年, 至 2020年
• 基盤研究(A)
  多重同位体標識窒素化合物（MILNC）による超高精度窒素循環解析
  自 2019年, 至 2019年
• 基盤研究(A)
  多重同位体標識窒素化合物（MILNC）による超高精度窒素循環解析
  自 2018年, 至 2018年
• 基盤研究(B)
  バイオフィルム内のＮ２Ｏ生成・消費機構の解明と排出削減が可能な排水処理技術の開発
  自 2017年, 至 2019年
• 基盤研究(A)
  高圧ジェット装置を用いた活性汚泥減量化機構の解明
  自 2017年, 至 2017年
• 挑戦的萌芽研究
  集積培養装置の革新によるnosZcladeIIタイプN2O還元細菌の獲得
  自 2016年, 至 2017年
• 基盤研究(B)
  藻類の水熱液化による栄養素循環利用型高効率バイオディーゼル合成法とシステム設計
  自 2016年, 至 2016年
• 基盤研究(B)
  新規なサスペンションゲル化法による微生物・酵素固定化多孔質ゲルの創製
  自 2016年, 至 2016年
• 基盤研究(A)
  高圧ジェット装置を用いた活性汚泥減量化機構の解明
  自 2016年, 至 2016年
• 基盤研究(B)
  藻類の水熱液化による栄養素循環利用型高効率バイオディーゼル合成法とシステム設計
  自 2015年, 至 2015年
• 基盤研究(B)
  新規なサスペンションゲル化法による微生物・酵素固定化多孔質ゲルの創製
  自 2015年, 至 2015年
• 基盤研究(A)
  高圧ジェット装置を用いた活性汚泥減量化機構の解明
  自 2015年, 至 2015年
• 基盤研究（B）
  実下水処理施設における亜酸化窒素の生成機構・排出動態の解明と対策手法の提示
  自 2015年, 至 2015年
• 若手研究(A)(一部基金)
  N原子と電子フローに着目したアンモニア酸化細菌のN2O生成機構解明と診断技術構築
  自 2014年, 至 2016年
• 挑戦的萌芽研究
  ガス透過膜デバイスによる亜酸化窒素還元細菌の集積化・単離
  自 2014年, 至 2015年
• 基盤研究（Ｂ）
  藻類の水熱液化による栄養素循環利用型高効率バイオディーゼル合成法とシステム設計
  自 2014年, 至 2014年
• 基盤研究（Ｂ）
  新規なサスペンションゲル化法による微生物・酵素固定化多孔質ゲルの創製
  自 2014年, 至 2014年
• 基盤研究（Ａ）
  高圧ジェット装置を用いた活性汚泥減量化機構の解明
  自 2014年, 至 2014年
• 挑戦的萌芽研究
  高圧ジェットによる活性汚泥微生物叢の制御可能性
  自 2013年, 至 2013年
• 基盤研究(B)
  新規微生物反応の制御による環境低負荷型畜産排水処理技術
  自 2013年, 至 2013年
• 挑戦的萌芽研究
  高圧ジェットによる活性汚泥微生物叢の制御可能性
  自 2012年, 至 2012年
• 基盤研究(C)
  イネ根圏におけるＡｎａｍｍｏｘ細菌の定量及び水田窒素循環への寄与の解明
  自 2012年, 至 2012年
• 基盤研究(B)
  新規微生物反応の制御による環境低負荷型畜産排水処理技術
  自 2012年, 至 2012年
• 挑戦的萌芽研究
  膜分離活性汚泥法の膜の目詰まりを抑制する酵素固定化型ろ過膜の開発
  自 2011年, 至 2011年
• 基盤研究(C)
  イネ根圏におけるＡｎａｍｍｏｘ細菌の定量及び水田窒素循環への寄与の解明
  自 2011年, 至 2011年
• 基盤研究(B)
  新規微生物反応の制御による環境低負荷型畜産排水処理技術
  自 2011年, 至 2011年
• 若手研究(B)
  アナモックス反応を短期間で既存の排水処理施設に導入可能なスタートアップ技術の開発
  自 2010年, 至 2012年
• 挑戦的萌芽研究
  膜分離活性汚泥法の膜の目詰まりを抑制する酵素固定化型ろ過膜の開発
  自 2010年, 至 2010年
• 基盤研究(C)
  イネ根圏におけるＡｎａｍｍｏｘ細菌の定量及び水田窒素循環への寄与の解明
  自 2010年, 至 2010年
• 基盤研究(B)
  新規培養法と安定同位体プローブ法で迫るN2O還元細菌の新機能：排出削減と物質産生
• 国際共同研究加速基金（国際共同研究強化(B)）
  集積培養技術とオミックス解析の融合が拓く亜酸化窒素還元細菌の獲得と応用

論文

• Effectiveness of biofilm scouring in improving the carbon and nitrogen removal performance of membrane-aerated biofilm reactors installing novel high oxygen-transfer polyethylene membranes
  Hiroto Miura, Yunje Kigo, Akihiko Terada
  Journal of Water Process Engineering
  Elsevier
  2024年03月01日, 研究論文（学術雑誌）, 共同, 59, DOI(公開)(r-map), 104880
• Enhanced granulation of activated sludge in an airlift reactor for organic carbon removal and ammonia retention from industrial fermentation wastewater: A comparative study
  Jingyu Duan, Kotaro Kitamura, Hiroki Tsukamoto, Hop Van Phan, Kohei Oba, Tomoyuki Hori, Taku Fujiwara, Akihiko Terada
  Water Research
  Elsevier
  2024年03月01日, 研究論文（学術雑誌）, 共同, 251, 0043-1354, DOI(公開)(r-map), 121091
• QsdS lactonase from Sphingopyxis sp. strain EG6 inhibits biofilm formation by Pseudomonas putida strain TS312 by degrading N-acyl homoserine lactone
  Ayaka Hosoe, Takanori Yoshino, Shogo Kashiwagi, Taro Iizumi, Airi Tanaka, Naohiro Nagai, Tomohiro Morohoshi, Akihiko Terada
  Environmental Technology & Innovation
  Elsevier
  2024年02月01日, 研究論文（学術雑誌）, 共同, 33, DOI(公開)(r-map), 103504
• Anti-biofouling performance and microbial communities of an integrated fixed-film activated sludge membrane bioreactor with a fibrous carrier material: Pilot-scale demonstration
  Hiroyuki Yoshino, Hop Van Phan, Naomichi Mori, Naoki Ohkuma, Masaki Kawakami, Masahiko Nihei, Satoshi Hashimoto, Ken Wakabayashi, Tomoyuki Hori, Akihiko Terada
  Science of The Total Environment
  Elsevier
  2024年01月23日, 研究論文（学術雑誌）, 共同, DOI(公開)(r-map), 170291
• Nitrate removal in iron sulfide-driven autotrophic denitrification biofilter: Biochemical and chemical transformation pathways and its underlying microbial mechanism
  Yang Bai, Huanhuan Hu, Po-Heng Lee, Ainur Zhussupbekova, Igor V Shvets, Bang Du, Akihiko Terada, Xinmin Zhan
  Science of The Total Environment
  Elsevier
  Iron sulfides-based autotrophic denitrification (IAD) is effective for treating nitrate-contaminated wastewater. However, the complex nitrate transformation pathways coupled with sulfur and iron cycles in IADs are still unclear. In this study, two columns (abiotic vs biotic) with iron sulfides (FeS) as the packing materials were constructed and operated continuously. In the abiotic column, FeS chemically reduced nitrate to ammonium under the ambient condition; this chemical reduction reaction pathway was spontaneous and has been over-looked in IAD reactors. In the biotic column (IAD biofilter), the complex nitrogen-transformation network was composed of chemical reduction, autotrophic denitrification, dissimilatory nitrate reduction to ammonium (DNRA) and sulfate reducing ammonium oxidation (Sulfammox). Metagenomic analysis and XPS characterization of the IAD biofilter further validated the roles of functional microbial communities (e.g., Acidovorax, Diaphorobacter, Desulfuromonas) in nitrate reduction process coupled with iron and sulfur cycles. This study gives an in-depth insight into the nitrogen transformations in IAD system and provides fundamental evidence about the underlying microbial mechanism for its further application in biological nitrogen removal.
  2023年11月25日, 研究論文（学術雑誌）, 共同, 901, 0048-9697, DOI(公開)(r-map), 165908
• Membrane fouling during nutrient recovery from digestate using electrodialysis: Impacts of the molecular size of dissolved organic matter
  Jizhong Meng, Lin Shi, Shun Wang, Zhenhu Hu, Akihiko Terada, Xinmin Zhan
  Journal of Membrane Science
  Elsevier
  Electrodialysis (ED) has shown application potential in nutrient recovery from digestate thanks to the frequent electrode reversal practice that can clean the ion-exchange membranes regularly. However, the fouling caused by dissolved organic matter (DOM) is still a challenge to the long-term operation of ion-exchange membranes in ED, and the mechanism of this fouling has not been well studied regarding these compounds' molecular size. Taking pig manure digestate as an example, this study utilized an ultrafiltration-ED design to fractionate the DOM into many different ranges of molecular size and then assessed the molecule size-specific DOM-fouling to ED membranes. The results showed that the fouling to the anion-exchange membrane (AM) was significantly influenced by molecular size, while the cation-exchange membrane was antifouling over full molecular size ranges. The DOM with a molecular size of smaller than 10 kDa, accounting for 9.1% of the total DOM in digestate, was found to mainly cause AM deterioration. In particular, the DOM smaller than 1 kDa migrated into the interior of AM, thus increasing the membrane resistance by 25.7% and decreasing the ion exchange capacity by 3.5%. The DOM with molecular size in the range of 1-10 kDa caused the fouling in the zone of 0-100 & mu;m depth from the membrane surface. Meanwhile, the DOM with molecular size larger than 10 kDa did not cause obvious irreversible fouling. For instance, the DOM being larger than 0.1 & mu;m, accounting for 75.2% of the total organic matter in the digestate, did not cause irreversible membrane fouling. These results indicate that attentions should be paid to the fouling caused by DOM with small molecular size of less than 10 kDa.
  2023年11月05日, 研究論文（学術雑誌）, 共同, 685, 0376-7388, DOI(公開)(r-map), 121974
• Evaluation of nitrous oxide emission during ammonia retention from simulated industrial wastewater by microaerobic activated sludge process
  Zhou, Xinyi Fujiwara, Taku Hidaka, Taira Nishimura, Fumitake Nakanishi, Tomohiro Terada, Akihiko Hori, Tomoyuki
  Water Research
  Elsevier
  Considering the reciprocating processes of nitrogen gas (N2) fixation to ammonia (NH4-N) and NH4-N removal to N2 through nitrification and denitrification during wastewater treatment, a microaerobic activated sludge process (MAS) is proposed in this study as a pretreatment to retain NH4-N from high-strength nitrogenous wastewater for further NH4-N recovery through membrane technology, that is, inhibit nitrification, with sufficient removal of total organic carbon (TOC). With DO and pH control, the 3-reactor bench-scale MAS systems successfully realized an NH4-N retention rate of over 80 %, with TOC removal rates of over 90 %. In addition, the emissions of carbon dioxide (CO2) and nitrous oxide (N2O) during MAS were evaluated. The total N2O emissions were 407 and 475 mg-N/day when pH was controlled at 6.2 (S1) and 6.8 (S2), respectively, with average emission factors to total nitrogen load over 2 % in both systems. Also, the global warming potential of N2O is one order of magnitude larger than that of CO2, indicating the significance of N2O in the MAS process. Therefore, the mechanisms of N2O emission from each reactor were investigated. The first reactor, where most of the TOC was adsorbed, emitted only 1.98 % (S1) and 2.43 % (S2) of the total N2O emissions through the denitrification of nitrite and nitrate (NOx) from the return sludge. The second reactor emitted 79.9 % (S1) and 69.0 % (S2) of the total N2O with the emission rates the same order of magnitude as the NOx production rates. Multiple pathways were considered to contribute to the high N2O emissions, and biotic NH2OH oxidation was one potential pathway at pH 6.2. Finally, the third reactor emitted 9.98 % (S1) and 16.8 % (S2) of the total N2O by nitrifier denitrification. Overall, this study showed that the large N2O emissions under nitrification-inhibiting conditions of the MAS process owed to the incomplete nitrification under acidic conditions and large abundances of denitrifiers. On the other hand, the lower N2O emissions at pH 6.2 evidenced the potential N2O mitigation under slightly more acidic conditions, underlining the necessity of further study on N2O mitigation when adapting to the trend of NH4-N recovery.
  2023年10月28日, 研究論文（学術雑誌）, 共同, 247, 0043-1354, DOI(公開)(r-map), 120780
• Complete genome sequence of Marinobacter shengliensis D49 harboring ectABC genes for ectoine synthesis
  Shohei Yasuda, Akihiko Terada
  Microbiology Resource Announcements
  American Society for Microbiology
  A complete genome sequence of Marinobacter shengliensis D49 in the class Gamma-proteobacteria was isolated from activated sludge treating landfill leachate. The genome encodes the functional genes for the biosynthesis of ectoine (ectABC), a compatible solute for cosmetics. Deciphering the genome helps pave the way for ectoine production by the isolate.
  2023年09月21日, 研究論文（学術雑誌）, 共同, 12, 10, 2576-098X, DOI(公開)(r-map), e00414-23
• バイオフィルムを用いた水処理技術：高度化と多機能化の潮流
  寺田昭彦
  アグリバイオ
  北隆館
  2023年09月20日, （MISC）総説・解説（商業誌）, 単独, 7, 10, 28, 33
• Microaerophilic Activated Sludge System for Ammonia Retention toward Recovery from High-Strength Nitrogenous Wastewater: Performance and Microbial Communities
  Hiroki Tsukamoto, Hop V Phan, Toshikazu Suenaga, Shohei Yasuda, Megumi Kuroiwa, Shohei Riya, Atsushi Ogata, Tomoyuki Hori, Akihiko Terada
  Environmental Science & Technology
  American Chemical Society
  A transition to ammonia recovery from wastewater has started; however, a technology for sustainable nitrogen retention in the form of ammonia and organic carbon removal is still in development. This study validated a microaerophilic activated sludge (MAS) system to efficiently retain ammonia from high-strength nitrogenous wastewater. The MAS is based on conventional activated sludge (CAS) with aerobic and settling compartments. Low dissolved oxygen (DO) concentrations (< 0.2 mg/L) and short solids retention times (SRTs) (< 5 days) eliminated nitrifying bacteria. The two parallel MASs were successfully operated for 300 days and had ammonia retention of 101.7 +/- 24.9% and organic carbon removal of 85.5 +/- 8.9%. The MASs mitigated N2O emissions with an emission factor of < 0.23%, much lower than the default value of CAS (1.6%). A short-term step-change test demonstrated that N2O indicated the initiation of nitrification and the completion of denitrification in the MAS. The parallel MASs had comparable microbial diversity, promoting organic carbon oxidation while inhibiting ammonia-oxidizing microorganisms (AOM(s)), as revealed by 16S rRNA gene amplicon sequencing, the quantitative polymerase chain reaction of functional genes, and fluorescence in situ hybridization of ss-proteobacteria AOB. The microbial analyses also uncovered that filamentous bacteria were positively correlated with effluent turbidity. Together, controlling DO and SRT achieved organic carbon removal and successful ammonia retention, mainly by suppressing AOM activity. This process represents a new nitrogen management paradigm.
  2023年09月07日, 研究論文（学術雑誌）, 共同, 57, 37, 0013-936X, DOI(公開)(r-map), 13874, 13886
• Particulate organic carbon potentially increases methane emissions from oxic water of eutrophic lakes
  Chuanqiao Zhou, Muchun Zhou, Ruoyu Jia, Yu Peng, Fenjun Zhao, Runze Xu, Shuoyuan Liang, Akihiko Terada, Guoxiang Wang, Tsuyoshi Kinouchi, Xiaoguang Xu
  Science of The Total Environment
  Elsevier
  Lakes are hot spots for methane (CH4) emissions and particulate organic carbon (POC) production, which describes the methane paradox phenomenon. However, the current understanding of the source of POC and its effect on CH4 emissions during eutrophication remains unclear. In this study, 18 shallow lakes in different trophic states were selected to investigate the POC source and its contribution to CH4 production, particularly to reveal the underlying mechanisms of the methane paradox. The carbon isotopic analysis showed that the delta 13Cpoc ranged from -30.28 %o to -21.14 %o, indicating that cyanobacteria-derived carbon is an important source of POC. The overlying water was aerobic but contained high concentrations of dissolved CH4. Particularly, in hyper-eutrophic lakes, such as Lakes Taihu, Chaohu, and Dianshan, the dissolved CH4 concentrations were 2.11, 1.01, and 2.44 mu mol/L, while the dissolved oxygen concentrations were 3.11, 2.92, and 3.17 mg/L, respectively. The intensified eutrophication increased the POC concentration, concomitantly promoting the dissolved CH4 concentration and the CH4 flux. These correlations revealed the role of POC in CH4 production and emission fluxes, particularly as a possible cause of the methane paradox, which is crucial for accurately evaluating the carbon budget and balance in shallow freshwater lakes.
  2023年09月01日, 研究論文（学術雑誌）, 共同, 889, 0048-9697, DOI(公開)(r-map), 164339
• 高濃度アンモニアを含む天然ガス・ヨウ素工場排水が流入する感潮河川における硝化特性
  星合靖夫 , 伴信一郎 , 芦澤広 , 工藤潤 , 三輪和範 , 日暮淳 , 山口秀幸 , 寺田昭彦 , 畑恭子 , 河野史郎
  水環境学会誌
  r 公益社団法人 日本水環境学会
  2023年09月, 研究論文（学術雑誌）, 共同, 45, 3, DOI(公開)(r-map), 91, 105
• Preparation of a poly(2-acrylamido-2-methyl-1-propanesulfonic acid)-grafted polyurethane sponge using atmospheric pressure plasma-induced graft polymerization and its catalytic properties for the acetalization of glycerol to solketal
  Hideaki Tokuyama, Hikaru Suzuki, Akihiko Terada, Hidenori Ohashi
  Reactive and Functional Polymers
  Elsevier
  Poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (poly(AMPS)) bearing a sulfo group was grafted onto a polyurethane sponge by an argon atmospheric-pressure plasma treatment and subsequent graft polymerization. Thus, a novel poly(AMPS)-grafted polyurethane sponge was developed as a heterogeneous acid catalyst for organic synthesis. The average grafting amount was 0.0277 kg-polymer/kg-substrate. The surface properties of the polyurethane sponge changed considerably, from hydrophobic to hydrophilic. The poly(AMPS)-grafted sponge successfully catalyzed the acetalization of glycerol with acetone to produce the desired solketal. The reaction rate was analyzed using a pseudo-first-order kinetic model, and the apparent reaction rate constant, k, and apparent activation energy were determined. The k value for the poly(AMPS)-grafted sponge was one order of magnitude larger than that for the EDGA-co-AMPS gel (EDGA: ethoxy diethyleneglycol acrylate). The poly (AMPS)-grafted sponge with interconnected submillimeter-sized macropores and high porosity (98%) exhibits excellent diffusional permeability. In addition, the structural features of the sponge provide advantages such as a negligible pressure drop and easy handling during the reaction process. This study provides a strategy for the use of polymers grafted onto sponges as catalysts.
  2023年08月18日, 研究論文（学術雑誌）, 共同, 191, 1381-5148, DOI(公開)(r-map), 105697
• Space-for-time substitution leads to carbon emission overestimation in eutrophic lakes
  Muchun Zhou, Chuanqiao Zhou, Yu Peng, Ruoyu Jia, Wenpeng Zhao, Shuoyuan Liang, Xiaoguang Xu, Akihiko Terada, Guoxiang Wang
  Environmental Research
  Academic Press
  Lacustrine eutrophication is generally considered as an important contributor of carbon emissions to the at-mosphere; however, there is still a huge challenge in accuracy estimating carbon emissions from lakes. To test the effect of widely used space-for-time substitution on lake carbon emissions, this study monitored different pro-cesses of carbon emissions, including the carbon production potential, dissolved carbon concentrations, and carbon release fluxes in eight lakes along the trophic gradients on a spatial scale and the typical eutrophic Lake Taihu for one year on a temporal scale. Eutrophication promoted carbon production potential, dissolved carbon concentrations, and carbon release fluxes, especially for CH4. Trophic lake index (TLI) showed positive corre-lations with the CH4 production potential, dissolved CH4 concentrations, and CH4 release fluxes, and also pos-itive correlations with the CO2 production potential, dissolved CO2 concentrations, and CO2 release fluxes. The space-for-time substitution led to an overestimation for the influence of eutrophication on carbon emissions, especially the further intensification of lake eutrophication. On the spatial scale, the average CH4 production potential, dissolved CH4 concentrations and CH4 release fluxes in eutrophic lakes were 268.6, 0.96 mu mol/L, and 587.6 mu mol m - 2 center dot h- 1,respectively, while they were 215.8, 0.79 mu mol/L, and 548.6 mu mol m - 2 center dot h- 1on the temporal scale. Obviously, CH4 and CO2 emissions on the spatial scale were significantly higher than those on the temporal scale in eutrophic lakes. The primary influencing factors were the seasonal changes in the physicochemical environments of lake water, including dissolved oxygen (DO) and temperature. The CH4 and CO2 release fluxes showed negative correlations with DO, while temperature displayed positive correlations, respectively. These results suggest that the effects of DO and temperature on lake carbon emissions should be considered, which may be ignored during the accurate assessment of lake carbon budget via space-for-time substitution in eutrophic lakes.
  2023年02月15日, 研究論文（学術雑誌）, 共同, 219, 0013-9351, DOI(公開)(r-map), 115175
• Microaerophilic activated sludge system for ammonia retention from high-strength nitrogenous wastewater: Biokinetics and mathematical modeling
  Jingyu Duan, Hop Van Phan, Hiroki Tsukamoto, Tomoyuki Hori, Akihiko Terada
  Biochemical Engineering Journal
  Elsevier
  It is crucial to shift from conventional ammonia removal to recovery to solve environmental problems and resource scarcity. Recovering ammonia from high-strength nitrogenous wastewater can simultaneously save the energy and cost involved in ammonia oxidation, produce ammonia without CO2 emissions, and prevent N2O emissions. A technology for concentrating and recovering ammonia by retrofitting a conventional activated sludge (CAS) system is promising but faces significant challenges in oxidizing organic carbon but not ammonia in wastewater. This study employed biokinetic analysis and mathematical modeling to comprehensively assess the operation and effectiveness of a microaerophilic activated sludge (MAS) system for simultaneously removing chemical oxygen demand (COD) and retaining ammonia. We show that heterotrophic bacteria (HB) in the MAS system had higher maximum specific growth rate (mu HB), biomass yield coefficient (YHB), and decay coefficient (bHB) but lower affinities for oxygen and COD than HB in CAS. Simulations using the developed model demonstrated that COD removal efficiencies of > 90%, ammonia retention efficiencies of > 90%, and low N2O emission factors (N2OEF) of < 0.01% can be achieved at solids retention times (SRTs) of 3-50 d, hydraulic retention times (HRTs) of 0.1-1 d, and a volumetric oxygen transfer coefficient (KLa) of (24.58 +/- 0.30)HRT-1. The combination of the biokinetics and mathematical modeling revealed the favorable MAS operating conditions allowing efficient ammonia retention and COD removal, which paves the way for turning a CAS system into a MAS system by retrofitting the configuration without high capital expenditures, requiring future intensive study.
  2023年02月01日, 研究論文（学術雑誌）, 共同, 191, 1369-703X, DOI(公開)(r-map), 108790
• Evaluation of Pollution Level, Spatial Distribution, and Ecological Effects of Antimony in Soils of Mining Areas: A Review
  Zhao, Shuting; Shi, Taoran; Terada, Akihiko; Riya, Shohei
  INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
  MDPI
  The first global-scale assessment of Sb contamination in soil that was related to mining/smelting activities was conducted based on 91 articles that were published between 1989 and 2021. The geographical variation, the pollution level, the speciation, the influencing factors, and the environmental effects of Sb that were associated with mining/smelting-affected soils were analyzed. The high Sb values mainly occurred in developed (Poland, Italy, Spain, Portugal, New Zealand, Australia) and developing (China, Algeria, Slovakia) countries. Sb concentrations of polluted soil from mining areas that were reported in most countries significantly exceeded the maximum permissible limit that is recommended by WHO, except in Turkey and Macedonia. The soil Sb concentrations decreased in the order of Oceania (29,151 mg/kg) > North Africa (13,022 mg/kg) > Asia (1527 mg/kg) > Europe (858 mg/kg) > South America (37.4 mg/kg). The existing extraction methods for Sb speciation have been classified according to the extractant, however, further research is needed in the standardization of these extraction methods. Modern analytical and characterization technologies, e.g., X-ray absorption spectroscopy, are effective at characterizing chemical speciation. Conditional inference tree (CIT) analysis has shown that the clay content was the major factor that influenced the soil Sb concentration. Non-carcinogenic risks to the public from soil Sb pollution were within the acceptable levels in most regions. An Sb smelter site at the Endeavour Inlet in New Zealand, an abandoned open-pit Sb mine in Djebel Hamimat, Algeria, an old Sb-mining area in Tuscany, Italy, and Hillgrove mine in Australia were selected as the priority control areas. Cynodon dactylon, Boehmeria, Pteris vittata, and Amaranthus paniculatus were found to be potential Sb accumulators. All of the values of bioaccumulation factors for the crops were less than one. However, ingestion of Sb through crop consumption posed potential non-carcinogenic health risks, which should not be neglected. The soil variables (pH, Eh, total sulfur, carbon nitrogen ratio, total organic carbon, and sulfate), the total Sb and the bioavailable Sb, and heavy metal(loid)s (As, Pb, and Fe) were the major parameters affecting the microbial community compositions.
  2023年01月, 研究論文（学術雑誌）, 共同, 20, 1, DOI(公開)(r-map)
• Evaluation of Pollution Level, Spatial Distribution, and Ecological Effects of Antimony in Soils of Mining Areas: A Review
  Shuting Zhao, Taoran Shi, Akihiko Terada, Shohei Riya
  International Journal of Environmental Research and Public Health
  MDPI
  2022年12月23日, 研究論文（学術雑誌）, 共同, 1, DOI(公開)(r-map), 242
• Identification of nosZ-expressing microorganisms consuming trace N2O in microaerobic chemostat consortia dominated by an uncultured Burkholderiales
  Kim, D.D., Han, H., Yun, T., Song, M.J., Terada, A., Laureni, M. and Yoon, S.
  The ISME Journal
  Springer Nature
  Microorganisms possessing N2O reductases (NosZ) are the only known environmental sink of N2O. While oxygen inhibition of NosZ activity is widely known, environments where N2O reduction occurs are often not devoid of O-2. However, little is known regarding N2O reduction in microoxic systems. Here, 1.6-L chemostat cultures inoculated with activated sludge samples were sustained for ca. 100 days with low concentration (<2 ppmv) and feed rate (<1.44 mu moles h(-1)) of N2O, and the resulting microbial consortia were analyzed via quantitative PCR (qPCR) and metagenomic/metatranscriptomic analyses. Unintended but quantified intrusion of O-2 sustained dissolved oxygen concentration above 4 mu M; however, complete N2O reduction of influent N2O persisted throughout incubation. Metagenomic investigations indicated that the microbiomes were dominated by an uncultured taxon affiliated to Burkholderiales, and, along with the qPCR results, suggested coexistence of clade I and II N2O reducers. Contrastingly, metatranscriptomic nosZ pools were dominated by the Dechloromonas-like nosZ subclade, suggesting the importance of the microorganisms possessing this nosZ subclade in reduction of trace N2O. Further, co-expression of nosZ and ccoNO/cydAB genes found in the metagenome-assembled genomes representing these putative N2O-reducers implies a survival strategy to maximize utilization of scarcely available electron acceptors in microoxic environmental niches.
  2022年09月, 研究論文（学術雑誌）, 共同, 16, 9, 1751-7362, DOI(公開)(r-map), 2087, 2098
• 水の化学 Part3 微生物を利用した水の浄化技術
  寺田昭彦
  化学と教育
  2022年08月20日, （MISC）総説・解説（学術雑誌）, 単独, 70, 8, 400, 403
• Exploring the Functions of Efficient Canonical Denitrifying Bacteria as N2O Sinks: Implications from N-15 Tracer and Transcriptome Analyses
  Oba, Kohei; Suenaga, Toshikazu; Kuroiwa, Megumi; Riya, Shohei; Terada, Akihiko
  ENVIRONMENTAL SCIENCE & TECHNOLOGY
  AMER CHEMICAL SOC
  In denitrifying reactors, canonical complete denitrifying bacteria reduce nitrate (NO3-) to nitrogen via N2O. However, they can also produce N2O under certain conditions. We used a N-15 tracer method, in which N-15-labeled NO3-/nitrite (NO2-) and nonlabeled N2O were simultaneously supplied with organic electron donors to five canonical complete denitrifying bacteria affiliated with either Clade I or Clade II nosZ. We calculated their NO3-, NO2-, and N2O consumption rates. The Clade II nosZ bacterium Azospira sp. strain I13 had the highest N2O consumption rate (3.47 +/- 0.07 fmol/cell/h) and the second lowest NO3- consumption rate (0.20 +/- 0.03 fmol/cell/h); hence, it is a N2O sink. A change from peptone- to acetate/citrate-based organic electron donors increased the NO3- consumption rate by 4.8 fold but barely affected the N2O consumption rate. Electron flow was directed to N2O rather than NO3- in Azospira sp. strain I13 and Az. oryzae strain PS only exerting a N2O sink but to NO3- in the Clade I nosZ N2O-reducing bacteria Pseudomonas stutzeri strain JCM 5965 and Alicycliphilus denitrificans strain I51. Transcriptome analyses revealed that the genotype could not fully describe the phenotype. The results show that N2O production and consumption differ among canonical denitrifying bacteria and will be useful for developing N2O mitigation strategies.
  2022年08月16日, 研究論文（学術雑誌）, 共同, 56, 16, 0013-936X, DOI(公開)(r-map), 11694, 11706
• Efficient oxygen supply and rapid biofilm formation by a new composite polystyrene elastomer membrane for use in a membrane-aerated biofilm reactor
  Kobayashi, M., Agari, R., Kigo, Y. and Terada, A.
  Biochemical Engineering Journal
  Elsevier
  A membrane-aerated biofilm reactor (MABR) provides bubbleless aeration and direct oxygen supply to a biofilm, achieving excellent aeration efficiency. Despite this advantage, research on membrane material is scarce. Here, we developed a composite membrane with high oxygen permeability and low vapor permeability with polystyrene elastomer (PS) as the intermediate non-porous layer sandwiched with porous polyethylene as the support layer. The oxygen transfer test demonstrated that the PS composite membrane provided 1.7 times higher oxygen transfer than the polyurethane (PU) composite membrane at an applied air pressure of 10 kPa. The membrane wettability test revealed that the PS composite membrane retained oxygen transfer capability. Two MABRs with the PU and PS composite hollow-fiber membranes, termed PU-MABR and PS-MABR, respectively, were continuously operated to treat organic carbon and nitrogen in synthetic wastewater. A higher carbon removal rate was obtained in PS-MABR [10.07?±?0.76?g-C/(m2?day)] than in PU-MABR [9.48?±?0.83?g-C/(m2?day)]. The superior performance was likely because of the higher oxygen utilization rate by the biofilm on the PS membrane [33.41?±?2.77?g-O2/(m2?day)] than on the PU membrane [25.67?±?7.36?g-O2/(m2?day)]. Moreover, PS-MABR allowed faster biofilm formation than PU-MABR. The new PS composite membrane resulted in effective oxygen supply, rapid startup, and high carbon removal by an MABR.
  2022年06月01日, 研究論文（学術雑誌）, 共同, 183, 1369-703X, DOI(公開)(r-map), 108442
• Low nitrous oxide concentration and spatial microbial community transition across an urban river affected by treated sewage
  Yiwen Zhou, Risako Toyoda, Toshikazu Suenaga, Tomo Aoyagi, Tomoyuki Hori, Akihiko Terada
  Water Research
  PERGAMON-ELSEVIER SCIENCE LTD
  Urban rivers receive used water derived from anthropogenic activities and are a crucial source of the potent greenhouse gas nitrous oxide (N2O). However, considerable uncertainties still exist regarding the variation and mechanisms of N2O production in response to the discharge of treated sewage from municipal wastewater treatment plants (WWTPs). This study investigated N2O concentrations and microbial processes responsible for nitrogen conversion upstream and downstream of WWTPs along the Tama River flowing through Tokyo, Japan. We evaluated the effect of treated sewage on dissolved N2O concentrations and inherent N2O consumption ac-tivities in the river sediments. In summer and winter, the mean dissolved N2O concentrations were 0.67 mu g-N L-1 and 0.82 mu g-N L-1, respectively. Although the dissolved N2O was supersaturated (mean 288.7% in summer, mean 240.7% in winter) in the river, the N2O emission factors (EF5r, 0.013%-0.025%) were significantly lower than those in other urban rivers and the Intergovernmental Panel on Climate Change default value (0.25%). The nitrate (NO3-) concentration in the Tama River increased downstream of the WWTPs discharge sites, and it was the main nitrogen constituent. An increasing trend of NO3- concentration was observed from upstream to downstream, along with an increase in the N2O consumption potential of the river sediment. A multiple regression model showed that NO3- is the crucial factor influencing N2O saturation. The diversity in the up-stream microbial communities was greater than that in the downstream ones, indicating the involvement of treated sewage discharge in shaping the microbial communities. Functional gene quantification for N2O pro-duction and consumption suggested that nirK-type denitrifiers likely contributed to N2O production. Structural equation models (SEMs) revealed that treated sewage discharged from WWTPs increased the NO3- loading from upstream to downstream in the river, inducing changes in the microbial communities and enhancing the N2O consumption activities. Collectively, aerobic conditions limited denitrification and in turn facilitated nitrifica-tion, leading to low N2O emissions even despite high NO3- loadings in the Tama River. Our findings unravel an overestimation of the N2O emission potential in an urban oxygen-rich river affected by treated sewage discharge.
  2022年06月01日, 研究論文（学術雑誌）, 共同, 216, 0043-1354, DOI(公開)(r-map), 118276
• 水処理システムの微生物群の全容を診る解析技術の進展
  寺田昭彦, 堀知行, 久保田健吾, 栗栖太, 春日郁朗, 金田一智規, 伊藤司
  水環境学会誌
  杏林社
  2022年06月, 研究論文（学術雑誌）, 共同, 45, 3, DOI(公開)(r-map), 91, 105
• Significance of soil moisture on temperature dependence of Hg emission
  S. Zhao, A. Terada, K. Nakamura, M. Nakashima, T. Komai, S. Riya, M. Hosomi, H. Hou
  Journal of Environmental Management
  Elsevier
  Soil moisture is a key factor for mercury (Hg) emission from soil. Despite its significance for Hg emissions, the effect of soil moisture on Hg flux and fractions has not been thoroughly investigated. The objective of this study was to elucidate the influences of soil moisture and temperature on Hg fluxes from soils and Hg fractions. A kinetic study was performed to measure Hg emission fluxes of six soil samples under different temperature (T) (15 degrees C, 20 degrees C, 25 degrees C, 30 degrees C, and 35 degrees C) and moisture conditions (0%, 10%, and 20% added water). The results showed that the Hg fluxes increased with increases in T and soil moisture. A linear correlation was found between ln (Hg emission flux) and 1/T for the six soil samples at different moisture contents (R-2 = 0.73-0.99). The range of activation energy (Ea) values was 25.31-57.86 kJ/mol. The Hg fractions in soils of different moisture content were determined by a sequential extraction method. The results demonstrated that soil moisture affected the Hg fractions in soils. The Ea values had different relationships with soil moisture in different soils. There were correlations between Ea and the elemental and mercuric sulfide fractions for air-dried soils. However, for moist soils, Ea was negatively correlated with the water-soluble and acid-soluble fractions. Collectively, the combination of the Hg emission kinetics and Hg fraction measurement of different moist soils indicated that Hg emission was affected by both total Hg concentration and Hg fractions.
  2022年03月, 研究論文（学術雑誌）, 共同, 305, 0301-4797, DOI(公開)(r-map), 114308
• Organic carbon determines nitrous oxide consumption activity of clade I and II nosZ bacteria: Genomic and biokinetic insights
  Chuang Qi, Yiwen Zhou, Toshikazu Suenaga, Kohei Oba, Jilai Lu, Guoxiang Wang, Limin Zhang, Sukhwan Yoon, Akihiko Terada
  Water Research
  PERGAMON-ELSEVIER SCIENCE LTD
  Harnessing nitrous oxide (N2O)-reducing bacteria is a promising strategy to reduce the N2O footprint of engineered systems. Applying a preferred organic carbon source as an electron donor accelerates N2O consumption by these bacteria. However, their N2O consumption potential and activity when fed different organic carbon species remain unclear. Here, we systematically compared the effects of various organic carbon sources on the activity of N2O-reducing bacteria via investigation of their biokinetic properties and genomic potentials. Five organic carbon sources-acetate, succinate, glycerol, ethanol, and methanol-were fed to four N2O-reducing bacteria harboring either clade I or clade II nosZ gene. Respirometric analyses were performed with four N2O-reducing bacterial strains, identifying distinct shifts in DO- and N2O-consumption biokinetics in response to the different feeding schemes. Regardless of the N2O-reducing bacteria, higher N2O consumption rates, accompanied by higher biomass yields, were obtained with acetate and succinate. The biomass yield (15.45 +/- 1.07 mgbiomass mmol-N2O-1) of Azospira sp. strain I13 (clade II nosZ) observed under acetate-fed condition was significantly higher than those of Paracoccus denitrificans and Pseudomonas stutzeri, exhibiting greater metabolic efficiency. However, the spectrum of the organic carbon species utilizable to Azospira sp. strain I13 was limited, as demonstrated by the highly variable N2O consumption rates observed with different substrates. The potential to metabolize the supplemented carbon sources was investigated by genomic analysis, the results of which corroborated the N2O consumption biokinetics results. Moreover, electron donor selection had a substantial impact on how N2O consumption activities were recovered after oxygen exposure. Collectively, our findings highlight the importance of choosing appropriate electron donor additives for increasing the N2O sink capability of biological nitrogen removal systems.
  2022年02月01日, 研究論文（学術雑誌）, 共同, 209, 1, 0043-1354, DOI(公開)(r-map), 117910
• Nitrous oxide-sink capability of denitrifying bacteria impacted by nitrite and pH
  Zhou, Y., Zhao, S., Suenaga, T., Kuroiwa, M., Riya, S. and Terada, A.
  Chemical Engineering Journal
  Elsevier
  Nitrous oxide (N2O) is a highly potent greenhouse gas and ozone-depleting substance, produced and consumed during denitrification. Evaluation of the N2O production and consumption activities of complete denitrifying bacteria is essential for understanding their capacity to act as N2O sinks in engineered systems for cost-effective nitrogen removal via nitrite (NO2-). However, the physiologies of these N2O-reducing bacteria (N2ORB) are poorly understood. This study aimed to evaluate the physiologies of two N2ORB, Azospira sp. strain I13 and Alicycliphilus denitrificans strain I51. A N-15 tracer method was applied to determine N2O production and consumption activities in the co-presence of NO2- and N2O. Both N2ORB displayed a higher N2O consumption rate (RN2O by Azospira sp. strain I13 and Alicycliphilus denitrificans strain I51, 23.85 and 7.60 mu mol-N mg -biomass - 1h(-1), respectively) than N2O production rate (PN2O, 5.88 and 1.32 mu mol-N mg-biomass-1h(-1),respectively) at an initial NO2- concentration of 2.14 mmol-N L-1 with exogenous addition of N2O, indicating that these N2ORB acted as N2O sinks. On increasing the NO2- concentration from 0.36 to 7.14 mmol-N L-1, the net N2O consumption rate R-O_N2O (=R-N2O - P-N2O) decreased for both N2ORB; the magnitude of the decrease was greater for Azospira sp. strain I13 than for Alicycliphilus denitrificans strain I51. The formation of free nitrous acid (FNA) from NO2- in acidic conditions noticeably affected the N2O sink activities of the N2ORB. A higher FNA concentration decreased R-O_N2O for both N2ORB, creating the risk of N2O emission at pH 6 and high NO2- con centration.Our results show the ranges of pH and NO2- concentration where Azospira sp. strain I13 is promising for use as an N2O sink in shortcut nitrogen removal via NO2- .
  2022年01月15日, 研究論文（学術雑誌）, 共同, 428, 1385-8947, DOI(公開)(r-map), 132402
• N2O Reduction by Gemmatimonas aurantiaca and Potential Involvement of Gemmatimonadetes Bacteria in N2O Reduction in Agricultural Soils
  Oshiki, Mamoru; Toyama, Yuka; Suenaga, Toshikazu; Terada, Akihiko; Kasahara, Yasuhiro; Yamaguchi, Takashi; Araki, Nobuo
  MICROBES AND ENVIRONMENTS
  JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
  Agricultural soil is the primary N2O sink limiting the emission of N2O gas into the atmosphere. Although Gemmatimonadetes bacteria are abundant in agricultural soils, limited information is currently available on N2O reduction by Gemmatimonadetes bacteria. Therefore, the effects of pH and temperature on N2O reduction activities and affinity constants for N2O reduction were examined by performing batch experiments using an isolate of Gemmatimonadetes bacteria, Gemmatimonas aurantiaca (NBRC100505(T)). G. aurantiaca reduced N2O at pH 5-9 and 4-50 degrees C, with the highest activity being observed at pH 7 and 30 degrees C. The affinity constant of G. aurantiaca cells for N2O was 4.4 mu M. The abundance and diversity of the Gemmatimonadetes 16S rRNA gene and nosZ encoding nitrous oxide reductase in agricultural soil samples were also investigated by quantitative PCR (qPCR) and amplicon sequencing analyses. Four N2O-reducing agricultural soil samples were assessed, and the copy numbers of the Gemmatimonadetes 16S rRNA gene (clades G1 and G3), nosZ DNA, and nosZ mRNA were 8.62-9.65x10(8), 5.35-7.15x10(8), and 2.23-4.31x10(9) copies (g dry soil)-1, respectively. The abundance of the nosZ mRNA of Gemmatimonadetes bacteria and OTU91, OUT332, and OTU122 correlated with the N2O reduction rates of the soil samples tested, suggesting N2O reduction by Gemmatimonadetes bacteria. Gemmatimonadetes 16S rRNA gene reads affiliated with OTU4572 and OTU3759 were predominant among the soil samples examined, and these Gemmatimonadetes OTUs have been identified in various types of soil samples.
  2022年, 研究論文（学術雑誌）, 共同, 37, 2, 1342-6311, DOI(公開)(r-map)
• Increased nitrogen deposition contributes to plant biodiversity loss in Japan: Insights from long-term historical monitoring data
  Lin, Bin-Le; Kumon, Yoko; Inoue, Kazuya; Tobari, Naoko; Xue, Mianqiang; Tsunemi, Kiyotaka; Terada, Akihiko
  ENVIRONMENTAL POLLUTION
  ELSEVIER SCI LTD
  Atmospheric deposition of reactive nitrogen compounds (Nrs) has been recognized as a threat to plant diversity in terrestrial ecosystems. As a first attempt to investigate the relationship between Nrs deposition and plant diversity loss in Japan, we collected and analyzed the available long-term nationwide monitoring data on annual Nrs deposition and plant (tree) species, and evaluated the relationship between Nrs deposition and plant species loss at corresponding sites. Analyses of the available data showed that the amount of Nrs deposited annually tended to decrease at two monitoring sites (Yusuhara, Hedomisaki) and increase at six monitoring sites across Japan (Rishiri, Sadoseki, Tokyo, Aichi, Oki, and Ogasawara) during the late 1980s to 2011, especially at Aichi (11.8-21.6 kgN.ha(-1).yr(-1)), Tokyo (10.0-23.5 kgN.ha(-1).yr(-1)), Oki (6.63-14.1 kgN.ha(-1).yr(-1)), and Rishiri (4.52-7.82 kgN.ha(-1).yr(-1)). Another long-term study, the Monitoring Sites 1000 Project, investigated the growth of tree species at 20 core sites across Japan during 2004-2012. The sites with higher potential plant diversity loss were close to those sites where Nrs deposition had markedly increased over the 20 years, such as Tokyo, Aichi, and Oki. Analyses of long-term monitoring data for tree species in the Tokyo University Forest in Aichi revealed that 22 of the 273 tree species (8.05%) disappeared during the period of 1990-2010, and twelve out of the 22 lost species were shrub species less than 5 m tall. Although our study obviously has some limitations in quantitatively presenting the relationship between the loss of plant diversity and increased atmospheric Nrs deposition in Japan, our findings provide evidence for this relationship based on analyses of historical nationwide monitoring data. These findings will be useful for establishing N critical loads for Japanese forests.
  2021年12月01日, 研究論文（学術雑誌）, 共同, 290, 0269-7491, DOI(公開)(r-map), 118033
• Microbial Biotransformation and Biomineralization of Organic-Rich Waste
  Ahmad, Wan Azlina; Abd Latif, Nurzila; Zaidel, Dayang Norulfairuz Abang; Ghazi, Rozidaini Mohd; Terada, Akihiko; Aguilar, Cristobal Noe; Zakaria, Zainul Akmar
  CURRENT POLLUTION REPORTS
  SPRINGER HEIDELBERG
  Purpose of Review Improper discharge of industrial effluents would lead to direct contamination of our water, air, and soil systems. Without proper treatment, both these inorganic and organic-matter-containing waste would pose harmful effects towards aquatic organisms, overall water quality, reduction in soil health, and increase in greenhouse gasses from anaerobic microbial degradation activities. Recent Findings Current treatment technologies involve the use of combined chemical, biological, and physical approaches, which has been proven very effective. Another useful alternative is to utilize the high organic content present in the waste as substrate for the metabolism of microbes as catalyst in industrial processes including water treatment as well as production of useful microbial secondary metabolites such as pigments. Summary This review highlights some example for the microbial biotransformation and biomineralization of organic-rich industrial discharges. This is important based on its potential to be applied as useful alternative techniques to dispose huge volumes of industrial waste as well as reducing high cost of sustaining biological-based industrial processes that would require substantial investment notably for the microbial growth medium. Nevertheless, clear insight into the engineering aspects of such processes and sufficient knowledge on its feasibility to function properly at pilot-scale level are of paramount importance prior to any commercialization attempts.
  2021年12月, 研究論文（学術雑誌）, 共同, 7, 4, 2198-6592, DOI(公開)(r-map), 435, 447
• Nonlinear pattern and algal dual-impact in N2O emission with increasing trophic levels in shallow lakes
  Zhou, Yiwen; Xu, Xiaoguang; Song, Kang; Yeerken, Senbati; Deng, Min; Li, Lu; Riya, Shohei; Wang, Qilin; Terada, Akihiko
  WATER RESEARCH
  PERGAMON-ELSEVIER SCIENCE LTD
  Shallow lakes are considered important contributors to emissions of nitrous oxide (N2O), a powerful greenhouse gas, in aquatic ecosystems. There is a large degree of uncertainty regarding the relationship between N2O emissions and the progress of lake eutrophication, and the mechanisms underlying N2O emissions are poorly understood. Here, N2O emission fluxes and environmental variables in different lakes along a trophic state gradient in the Yangtze River basin were studied. N2O emission fluxes were -1.0-53.0 mu g m(-2) h(-)(1) and 0.4-102.9 mu g m(-2) h(-1) in summer and winter, respectively, indicating that there was marked variation in N2O emissions among lakes of different trophic state. The non-linear exponential model explained differences in N2O emission fluxes by the degree of eutrophication (p < 0.01). TN and chl-a both predicted 86% of the N2O emission fluxes in shallow lakes. The predicted N2O emission fluxes based on the IPCC EF5r overestimated the observed fluxes, particularly those in hyper-eutrophic lakes. These findings demonstrated that nutrient-rich conditions and algal accumulation are key factors determining N2O emission fluxes in shallow lakes. Furthermore, this study also revealed that temperature and algae accumulation-decomposition determine an N2O emission flux in an intricate manner. A low temperature, i.e., winter, limits algae growth and low oxygen consumption for algae decomposition. The environment leaves a high dissolved oxygen concentration, slowing down N2O consumption as the final step of denitrification. In summer, with the oxygen consumed by excess algal decomposition, the N2O production is limited by the complete denitrification as well as the limited substrate supply of nitrate by nitrification in hypoxic or anoxic conditions. Such cascading events explained the higher N2O emission fluxes from shallow lakes in winter compared with summer. This trend was amplified in hyper-eutrophic shallow lakes after algal disappearance. Collectively, algal accumulation played a dual role in stimulating and impeding N2O emissions, especially in hyper-eutrophic lakes. This study expands our knowledge of N2O emissions from shallow lakes in which eutrophication is underway.
  2021年09月15日, 研究論文（学術雑誌）, 共同, 203, 0043-1354, DOI(公開)(r-map)
• Combination of N-15 Tracer and Microbial Analyses Discloses N2O Sink Potential of the Anammox Community
  Suenaga, Toshikazu; Ota, Takumi; Oba, Kohei; Usui, Kentaro; Sako, Toshiki; Hori, Tomoyuki; Riya, Shohei; Hosomi, Masaaki; Chandran, Kartik; Lackner, Susanne; Smets, Barth F.; Terada, Akihiko
  ENVIRONMENTAL SCIENCE & TECHNOLOGY
  AMER CHEMICAL SOC
  Although nitrogen removal by partial nitritation and anammox is more cost-effective than conventional nitrification and denitrification, one downside is the production and accumulation of nitrous oxide (N2O). The potential exploitation of N2O-reducing bacteria, which are resident members of anammox microbial communities, for N2O mitigation would require more knowledge of their ecophysiology. This study investigated the phylogeny of resident N2O-reducing bacteria in an anammox microbial community and quantified individually the processes of N2O production and N2O consumption. An up-flow column-bed anammox reactor, fed with NH4+ and NO2- and devoid of oxygen, emitted N2O at an average conversion ratio (produced N2O: influent nitrogen) of 0.284%. Transcriptionally active and highly abundant nosZ genes in the reactor biomass belonged to the Burkholderiaceae (clade I type) and Chloroflexus genera (clade II type). Meanwhile, less abundant but actively transcribing nosZ strains were detected in the genera Rhodoferax, Azospirillum, Lautropia, and Bdellovibrio and likely act as an N2O sink. A novel N-15 tracer method was adapted to individually quantify N2O production and N2O consumption rates. The estimated true N2O production rate and true N2O consumption rate were 3.98 +/- 0.15 and 3.03 +/- 0.18 mg(N.gVSS)(-1).day(-1), respectively. The N2O consumption rate could be increased by 51% (4.57 +/- 0.51 mg(N.gVSS)(-1).day(-1)) with elevated N2O concentrations but kept comparable irrespective of the presence or absence of NO2-. Collectively, the approach allowed the quantification of N2O-reducing activity and the identification of transcriptionally active N2O reducers that may constitute as an N2O sink in anammox-based processes.
  2021年07月06日, 研究論文（学術雑誌）, 共同, 55, 13, 0013-936X, DOI(公開)(r-map), 9231, 9242
• Long-term Assessment of N2O Emission Factor in Full-scale Oxidation Ditch Reactor Considering Spatiotemporal Distribution
  Shohei Otomo, Akihiko Terada, Yu-You Li, Kazuya Nishitoba, Fumiaki Takakai, Kunihiro Okano, Naoyuki Miyata, Shuhei Masuda
  Journal of Water and Environment Technology
  Japan Society on Water Environment
  2021年06月10日, 研究論文（学術雑誌）, 共同, 19, 3, DOI(公開)(r-map), 139, 152
• Metagenomic Insights Into Functional and Taxonomic Compositions of an Activated Sludge Microbial Community Treating Leachate of a Completed Landfill: A Pathway-Based Analysis
  Yasuda, Shohei; Suenaga, Toshikazu; Orschler, Laura; Agrawal, Shelesh; Lackner, Susanne; Terada, Akihiko
  FRONTIERS IN MICROBIOLOGY
  FRONTIERS MEDIA SA
  Upcycling wastes into valuable products by mixed microbial communities has recently received considerable attention. Sustainable production of high-value substances from one-carbon (C1) compounds, e.g., methanol supplemented as an external electron donor in bioreactors for wastewater treatment, is a promising application of upcycling. This study undertook a gene-centric approach to screen valuable production potentials from mixed culture biomass, removing organic carbon and nitrogen from landfill leachate. To this end, the microbial community of the activated sludge from a landfill leachate treatment plant and its metabolic potential for the production of seven valuable products were investigated. The DNA extracted from the activated sludge was subjected to shotgun metagenome sequencing to analyze the microbial taxonomy and functions associated with producing the seven products. The functional analysis confirmed that the activated sludge could produce six of the valuable products, ectoine, polyhydroxybutyrate (PHB), zeaxanthin, astaxanthin, acetoin, and 2,3-butanediol. Quantification of the detected functional gene hit numbers for these valuable products as a primary trial identified a potential rate-limiting metabolic pathway, e.g., conversion of L-2,4-diaminobutyrate into N-gamma-acetyl-L2,4,-diaminobutyrate during the ectoine biosynthesis. Overall, this study demonstrated that primary screening by the proposed gene-centric approach can be used to evaluate the potential for the production of valuable products using mixed culture or single microbe in engineered systems. The proposed approach can be expanded to sites where water purification is highly required, but resource recovery, or upcycling has not been implemented.
  2021年04月30日, 研究論文（学術雑誌）, 共同, 12, 1664-302X, DOI(公開)(r-map), 948
• Temperature and oxygen level determine N2O respiration activities of heterotrophic N2O-reducing bacteria: Biokinetic study
  Zhou, Yiwen; Suenaga, Toshikazu; Qi, Chuang; Riya, Shohei; Hosomi, Masaaki; Terada, Akihiko
  BIOTECHNOLOGY AND BIOENGINEERING
  WILEY
  Nitrous oxide (N2O), a potent greenhouse gas, is reduced to N-2 gas by N2O-reducing bacteria (N2ORB), a process which represents an N2O sink in natural and engineered ecosystems. The N2O sink activity by N2ORB depends on temperature and O-2 exposure, yet the specifics are not yet understood. This study explores the effects of temperature and oxygen exposure on biokinetics of pure culture N2ORB. Four N2ORB, representing either clade I type nosZ (Pseudomonas stutzeri JCM5965 and Paracoccus denitrificans NBRC102528) or clade II type nosZ (Azospira sp. strains I09 and I13), were individually tested. The higher activation energy for N2O by Azospira sp. strain I13 (114.0 +/- 22.6 kJ mol(-1)) compared with the other tested N2ORB (38.3-60.1 kJ mol(-1)) indicates that N2ORB can adapt to different temperatures. The O-2 inhibition constants (K-I) of Azospira sp. strain I09 and Ps. stutzeri JCM5965 increased from 0.06 +/- 0.05 and 0.05 +/- 0.02 mu mol L-1 to 0.92 +/- 0.24 and 0.84 +/- 0.31 mu mol L-1, respectively, as the temperature increased from 15 degrees C to 35 degrees C, while that of Azospira sp. strain I13 was temperature-independent (p = 0.106). Within the range of temperatures examined, Azospira sp. strain I13 had a faster recovery after O-2 exposure compared with Azospira sp. strain I09 and Ps. stutzeri JCM5965 (p < 0.05). These results suggest that temperature and O-2 exposure result in the growth of ecophysiologically distinct N2ORB as N2O sinks. This knowledge can help develop a suitable N2O mitigation strategy according to the physiologies of the predominant N2ORB.
  2021年03月, 研究論文（学術雑誌）, 共同, 118, 3, 0006-3592, DOI(公開)(r-map), 1330, 1341
• Time to act-assessing variations in qPCR analyses in biological nitrogen removal with examples from partial nitritation/anammox systems
  Agrawal, Shelesh; Weissbrodt, David G.; Annavajhala, Medini; Jensen, Marlene Mark; Arroyo, Jose Maria Carvajal; Wells, George; Chandran, Kartik; Vlaeminck, Siegfried E.; Terada, Akihiko; Smets, Barth F.; Lackner, Susanne
  WATER RESEARCH
  PERGAMON-ELSEVIER SCIENCE LTD
  Quantitative PCR (qPCR) is broadly used as the gold standard to quantify microbial community fractions in environmental microbiology and biotechnology. Benchmarking efforts to ensure the comparability of qPCR data for environmental bioprocesses are still scarce. Also, for partial nitritation/anammox (PN/A) systems systematic investigations are still missing, rendering meta-analysis of reported trends and generic insights potentially precarious. We report a baseline investigation of the variability of qPCR-based analyses for microbial communities applied to PN/A systems. Round-robin testing was performed for three PN/A biomass samples in six laboratories, using the respective in-house DNA extraction and qPCR protocols. The concentration of extracted DNA was significantly different between labs, ranged between 2.7 and 328 ng mg(-1) wet biomass. The variability among the qPCR abundance data of different labs was very high (1-7 log fold) but differed for different target microbial guilds. DNA extraction caused maximum variation (3-7 log fold), followed by the primers (1-3 log fold). These insights will guide environmental scientists and engineers as well as treatment plant operators in the interpretation of qPCR data. (c) 2020 Elsevier Ltd. All rights reserved.
  2021年02月15日, 研究論文（学術雑誌）, 共同, 190, 0043-1354, DOI(公開)(r-map), 116604
• 浄化槽処理水の放流後から排出される温室効果ガス
  山崎宏史, 村野昭人, 青木宗之, 蛯江美孝, 小野寺崇, 増田周平, 寺田昭彦
  月刊浄化槽
  2021年02月10日, （MISC）総説・解説（商業誌）, 共同, 538, 1347-6866, 12, 16
• Oxygen diffusivity and reaction rate in spherical gel entrapping ammonia-oxidizing bacteria
  Aoyagi, Ryo; Terada, Akihiko; Tokuyama, Hideaki
  BIOCHEMICAL ENGINEERING JOURNAL
  ELSEVIER
  Nitrifying biomass immobilized in a gel has been applied in wastewater treatment, as immobilization results in a high bacterial cell density in the reactor and facilitates easy solid-liquid separation in the settling tank. Herein, the diffusivity, reaction rate, and concentration profile of oxygen in a spherical gel entrapping ammonia-oxidizing bacteria (AOB) were investigated. Calcium alginate gel and 2,2-bis [4-(methacryloxy polyethoxy) phenyl] propane (BPE) gel were used as models to prepare millimeter-sized spherical gels. A novel method involving controlled oxygen transfer into/out of the spherical gel with alternative flow of air and N-2 gas, measurement of oxygen concentration at the center of the spherical gel using an oxygen microsensor, and analysis using the Fickian diffusion equation was developed to determine oxygen diffusivity in the gel. Ammonia oxidation in the AOB-entrapping gel was also monitored by measuring the oxygen concentration at the center of the gel using an oxygen microsensor. The oxygen concentration profile in the gel was estimated based on the oxygen diffusivity and Michaelis-Menten model parameters determined for suspended AOB cells. The kinetic rate-determining step in the AOB-entrapping gel was identified using the effectiveness factor. The results would be useful for the development of aerobic bacteria-entrapping gels and the design of wastewater treatment processes using these gels.
  2020年12月15日, 研究論文（学術雑誌）, 共同, 164, 107788, 1369-703X, DOI(公開)(r-map)
• Nitrogen removal efficiencies and microbial communities in full-scale IFAS and MBBR municipal wastewater treatment plants at high COD:N ratio
  Phanwilai, Supaporn; Kangwannarakul, Naluporn; Noophan, Pongsak (Lek); Kasahara, Tamao; Terada, Akihiko; Munakata-Marr, Junko; Figueroa, Linda Ann
  FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING
  HIGHER EDUCATION PRESS
  The relative locations of AOB, NOB, and DNB were examined for three different kinds of carriers in two types of hybrid biofilm process configurations: integrated fixed-film activated sludge (IFAS) and moving bed biofilm reactor (MBBR) processes. IFAS water resource recovery facilities (WRRFs) used Anodkalness (TM) K1 carriers (KC) at Broomfield, Colorado, USA and polypropylene resin carriers (RC) at Fukuoka, Japan, while MBBR WRRFs used KC carriers at South Adams County, Colorado, UsA and sponge carriers (SC) at Saga, Japan. Influent COD to N ratios ranged from 8:1 to 15:1. The COD and BOD removal efficiencies were high (96%-98%); NH4+-N and TN removal efficiencies were more varied at 72%-98% and 64%-77%, respectively. The extent of TN removal was higher at high SRT, high COD:N ratio and low DO concentration in the anoxic tank. In IFAS, RC with high specific surface area (SSA) maintained higher AOB population than KC. Sponge carriers with high SSA maintained higher overall bacteria population than KC in MBBR systems. However, the DNB were not more abundant in high SSA carriers. The diversity of AOB, NOB, and DNB was fairly similar in different carriers. Nitrosomonas sp. dominated over Nitrosospira sp. while denitrifying bacteria included Rhodobacter sp., Sulfuritalea sp., Rubrivivax sp., Paracoccus sp., and Pseudomonas sp. The results from this work suggest that high SRT, high COD:N ratio, low DO concentration in anoxic tanks, and carriers with greater surface area may be recommended for high COD, BOD and TN removal in WRRFs with IFAS and MBBR systems. (c) Higher Education Press 2020
  2020年12月, 研究論文（学術雑誌）, 共同, 14, 6, 2095-2201, DOI(公開)(r-map), 115
• Nitrogen and Oxygen Isotope Signatures of Nitrogen Compounds during Anammox in the Laboratory and a Wastewater Treatment Plant
  Kotajima, Shotoku; Koba, Keisuke; Ikeda, Daisuke; Terada, Akihiko; Isaka, Kazuichi; Nishina, Kazuya; Kimura, Yuuya; Makabe, Akiko; Yano, Midori; Fujitani, Hirotsugu; Ushiki, Norisuke; Tsuneda, Satoshi; Yoh, Muneoki
  MICROBES AND ENVIRONMENTS
  JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE
  Isotopic fractionation factors against N-1(5) and O-18 during anammox (anaerobic ammonia oxidization by nitrite) are critical for evaluating the importance of this process in natural environments. We performed batch incubation experiments with an anammox-dominated biomass to investigate nitrogen (N) and oxygen (O) isotopic fractionation factors during anammox and also examined apparent isotope fractionation factors during anammox in an actual wastewater treatment plant. We conducted one incubation experiment with high delta O-1(8) of water to investigate the effects of water delta O-1(8). The N isotopic fractionation factors estimated from incubation experiments and the wastewater treatment plant were similar to previous values. We also found that the N isotopic effect ((15)epsilon(NXR) of -77.8 to -65.9 parts per thousand and (15)Delta(NXR) of -31.3 to -30.4 parts per thousand) and possibly O isotopic effect ((18)epsilon(NXR)( )of -20.6 parts per thousand) for anaerobic nitrite oxidation to nitrate were inverse. We applied the estimated isotopic fractionation factors to the ordinary differential equation model to clarify whether anammox induces deviations in the delta O-18 vs delta N-15 of nitrate from a linear trajectory of 1, similar to heterotrophic denitrification. Although this deviation has been attributed to nitrite oxidation, the O isotopic fractionation factor for anammox is crucial for obtaining a more detailed understanding of the mechanisms controlling this deviation. In our model, anammox induced the trajectory of the delta O-1(8) vs delta N-15 of nitrate during denitrification to less than one, which strongly indicates that this deviation is evidence of nitrite oxidation by anammox under denitrifying conditions.
  2020年11月07日, 研究論文（学術雑誌）, 共同, 35, 4, 1342-6311, DOI(公開)(r-map)
• Nonlinear response of methane release to increased trophic state levels coupled with microbial processes in shallow lakes
  Zhou, Yiwen; Song, Kang; Han, Ruiming; Riya, Shohei; Xu, Xiaoguang; Yeerken, Senbati; Geng, Shixiong; Ma, You; Terada, Akihiko
  ENVIRONMENTAL POLLUTION
  ELSEVIER SCI LTD
  Shallow lakes are a crucial source of methane (CH4), a potent greenhouse gas, to the atmosphere. However, large uncertainties still exist regarding the response of CH4 emissions to the increasing trophic levels of lakes as well as the underlying mechanisms. Here, we investigate the CH4 emission flux from lakes with different trophic states in the middle and lower reaches of the Yangtze River basin, China to evaluate the effect of the trophic lake index (TLI) on CH4 emissions. The mean CH4 emission fluxes from mesotrophic, eutrophic, middle-eutrophic, and hyper-eutrophic lakes were 0.1, 4.4, 12.0, and 130.4 mg m(-2)h(-1), respectively. Thus, the CH4 emission flux ranged widely and was positively correlated with the degree of eutrophication. The relative abundance of methanogens with respect to the total population for the mesotrophic, eutrophic, mid-eutrophic, and hyper-eutrophic states was 0.03%, 0.35%, 0.94%, and 1.17%, respectively. The biogeographic-scale pattern of lakes classified as each of these four trophic states indicated that CH4 emissions could be well-predicted by the NH4+-N concentration in the water column, as both NH4+-N and CH4 were produced during mineralisation of labile organic matter in lake sediment. In addition, the shift from clear to turbid water, which is an unhealthy evolution for lakes, was associated with a nonlinear increase in the CH4 emissions from the studied lakes. In particular, the hypereutrophic lakes functioned as CH4 emission hotspots. Our findings highlight that nutrient levels, as a potential facilitator of CH4 emissions, should be considered in future research to accurately evaluate the greenhouse gas emissions from shallow lakes. (C) 2020 Elsevier Ltd. All rights reserved.
  2020年10月01日, 研究論文（学術雑誌）, 共同, 265, 0269-7491, DOI(公開)(r-map), 114919
• Exploration and enrichment of methane-oxidizing bacteria derived from a rice paddy field emitting highly concentrated methane
  Yasuda, Shohei; Toyoda, Risako; Agrawal, Shelesh; Suenaga, Toshikazu; Riya, Shohei; Hori, Tomoyuki; Lackner, Susanne; Hosomi, Masaaki; Terada, Akihiko
  JOURNAL OF BIOSCIENCE AND BIOENGINEERING
  SOC BIOSCIENCE BIOENGINEERING JAPAN
  Methane-oxidizing bacteria (MOB) possess the metabolic potential to assimilate the highly potent greenhouse gas, CH4, and can also synthesize valuable products. Depending on their distinct and fastidious metabolic pathways, MOB are mainly divided into Type I and Type II; the latter are known as producers of polyhydroxyalkanoate (PHA). Despite the metabolic potential of MOB to synthesize PHA, the ecophysiology of MOB, especially under high CH4 flux conditions, is yet to be understood. Therefore, in this study, a rice paddy soil receiving a high CH4 flux from underground was used as an inoculum to enrich MOB using fed-batch operation, then the enriched Type II MOB were characterized. The transitions in the microbial community composition and CH4 oxidation rates were monitored by 16S rRNA gene amplicon sequencing and degree of CH4 consumption. With increasing incubation time, the initially dominant Methylomonas sp., affiliated with Type I MOB, was gradually replaced with Methylocystis sp., Type II MOB, resulting in a maximum CH4 oxidation rate of 1.40 g-CH4/g-biomass/day. The quantification of functional genes encoding methane monooxygenase, pmoA and PHA synthase, phaC, by quantitative PCR revealed concomitant increases in accordance with the Type II MOB enrichment. These increases in the functional genes underscore the significance of Type II MOB to mitigate greenhouse gas emission and produce PHA. (C) 2020, The Society for Biotechnology, Japan. All rights reserved.
  2020年09月, 研究論文（学術雑誌）, 共同, 130, 3, 1389-1723, DOI(公開)(r-map), 311, 318
• Complete Genome Sequence of Methylosinus sp. Strain C49, a Methane-Oxidizing Bacterium Harboring phaABC Genes for Polyhydroxyalkanoate Synthesis
  Yasuda S, Suenaga T, Terada A
  Microbiology Resource Announcements
  American Society for Science
  2020年09月, 研究論文（学術雑誌）, 共同, 9, 27, DOI(公開)(r-map), e00113, 20
• Eco-compatible biochar mitigates volatile fatty acids stress in high load thermophilic solid-state anaerobic reactors treating agricultural waste
  Meng, Lingyu; Xie, Li; Suenaga, Toshikazu; Riya, Shohei; Terada, Akihiko; Hosomi, Masaaki
  BIORESOURCE TECHNOLOGY
  ELSEVIER SCI LTD
  A high concentration of accumulated volatile fatty acids (VFAs) is one of the most important factors resulting in reactor failure during solid-state anaerobic digestion. In this study, the feedstock-to-inoculum (F/I) ratio (0.5, 2, 3, 4 and 6) and the recovery method after failure (biochar addition or inoculum addition) were investigated in batch solid-state anaerobic digestion fed with rice straw and pig urine. An F/I ratio of 3 was the threshold for stable operation, while the reactors failed at F/I ratios of 4 and 6 because of high accumulated VFAs concentrations (above 30 g HAc/kg). Biochar addition (10% or 20% (wet weight) of the mixture) was as effective as inoculum addition (by adjusting the F/I ratio to 2 or 3) in promoting VFAs degradation in failed reactors within a short period (< 1 day). The buffering capacity of biochar was important in promoting VFAs degradation.
  2020年08月01日, 研究論文（学術雑誌）, 共同, 309, 0960-8524, DOI(公開)(r-map), 123366
• Reducing geogenic arsenic leaching from excavated sedimentary soil using zero-valent iron amendment followed by dry magnetic separation: A case study
  Li, Jining; Yoshi, Shunsuke; Hashimoto, Yohey; Wang, Lei; Wang, Fenghe; Riya, Shohei; Terada, Akihiko; Hosomi, Masaaki
  SCIENCE OF THE TOTAL ENVIRONMENT
  ELSEVIER
  Although the deep-layer sedimentary soils excavated from construction sites contain low level of geogenic arsenic (As), remediation is necessary when the As leachability exceeds the environmental standard (10 mu g/L) in Japan. In this study, the zero-valent iron (ZVI) amendment followed by dry magnetic separation (ZVI-DMS) was implemented for the treatment of a geogenic As-contaminated alkaline sedimentary soil (pH 8.9; 7.5 mg/kg of total As; 0.33 mg/kg of water-extractable As). This technology involves pH adjustment (adding H2SO4), ZVI addition, water content reduction (adding water adsorbent CaSO4 center dot 0.5H(2)O), and dry magnetic separation. The short-term and long-term As leachability before and after treatment was compared using sequential water leaching tests (SWLT). The results illustrated that As could be removed from the bulk soil through the magnetic separation of As-ZVI complexes, although the amount was limited (about 2% of total As). Moreover, immobilization played a dominant role in suppressing As leaching. The H2SO4 addition decreased pH to a circumneutral range and thereby suppress As release. The CaSO4 center dot 0.5H(2)O addition also contributed to the pH decrease and reduced As leachability. Besides, CaSO4 center dot 0.5H(2)O-dissolution released Ca2+ that favored As adsorption, and enhanced dissolved organic carbon (DOC) coagulation that decelerated As dissolution. SWLT results indicated that As leachability from remediated soil satisfied the environmental standard (10 mu g/L) in both short-term and long-term perspective. However, the secular stability of treated soil deserves more attention due to the easy re-release of As caused by As-bearing framboidal pyrite oxidation. Additionally, during ZVI-DMS process, there is a need to scientifically decide the dosage of ZVI to avoid excessive addition. Our results demonstrated that ZVI-DMS technology could be a promising remediation strategy for geogenic As contaminated sedimentary soils/rocks. (C) 2020 Elsevier B.V. All rights reserved.
  2020年07月01日, 研究論文（学術雑誌）, 共同, 724, 0048-9697, DOI(公開)(r-map), 138203
• The implication of an advanced bioprocess for the acquisition of valuable microbial resources toward a sustainable and low-environmental burden society
  Terada, Akihiko
  CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY
  SPRINGER
  2020年07月01日, 研究論文（学術雑誌）, 単独, 22, 5, 1618-954X, DOI(公開)(r-map), 993, 994
• Quorum quenching acylase impacts the viability and morphological change of Agrobacterium tumefaciens cells
  Bao, Qian; Hosoe, Ayaka; Hosomi, Masaaki; Terada, Akihiko
  JOURNAL OF BIOSCIENCE AND BIOENGINEERING
  SOC BIOSCIENCE BIOENGINEERING JAPAN
  Acylase is known as a quorum quenching enzyme that degrades N-acyl-homoserine lactones (AHLs), a key signaling molecule in a quorum sensing (QS) mechanism. Acylase I cleaves the acyl-chain in the chemical structures of AHLs, thereby exerting an anti-biofilm effect by the inhibition of bacterial cellecell communication and resultant secretion of extracellular polymeric substances (EPS). However, the physical and physiological impacts of acylase on bacterial cells remain to be systematically elucidated. This study, therefore, investigated the effect of active and inactive acylase addition on the growth, viability, and cell morphologies of Agrobacterium tumefaciens. For comparison, active and inactive lysozymes were taken as positive controls. The results showed that active acylase inhibited A. tumefaciens cell growth at concentrations ranging from 0.1 to 1000 mu g mL(-1), and so did active lysozyme. Fluorescent detection by Live/Dead staining underpinned that cell viability of A. tumefaciens decreased at concentrations higher than 0.1 mu g mL(-1) for both acylase and lysozyme, although lysozyme inflicted higher degree of cellular damage. Moreover, atomic force microscopy unraveled a noticeable distortion of A. tumefaciens cells by both acylase and lysozyme. Together, the results showed that acylase not only blocked AHLs-based QS mechanisms but also compromised cell viability and altered surface morphology of A. tumefaciens cells, as observed by the addition of hydrolase. (C) 2020, The Society for Biotechnology, Japan. All rights reserved.
  2020年07月, 研究論文（学術雑誌）, 共同, 130, 1, 1389-1723, DOI(公開)(r-map), 82, 88
• Identifying prokaryotes and eukaryotes disintegrated by a high-pressure jet device for excess activated sludge reduction
  Yoshino, Hiroyuki; Hori, Tomoyuki; Hosomi, Masaaki; Terada, Akihiko
  BIOCHEMICAL ENGINEERING JOURNAL
  ELSEVIER
  Changes in microbial communities are observed during the long-term operation of activated sludge systems employing an excess sludge reduction technology. Short-term changes are also significant to confirm if key microbes are damaged, but they have not been investigated due to the absence of an appropriate method. We report a methodology to identify prokaryotic and eukaryotic species damaged almost instantaneously by a high-pressure jet device (HPJD), a cost-effective sludge reduction technology. 16S rRNA gene amplicon sequencing and microscopic inspection were implemented to identify damaged microorganisms. A sludge injection ratio (R), activated sludge suspension from the top port relative to that of the lateral port of the HPJD, was set at 0, 1, and 3. The DNA eluted by the HPJD treatment at these R-values was successfully retrieved. Nitrifying bacteria were not detected in the eluted DNA at the R-values, indicating that the essential guilds for nitrogen removal can be retained. The dominant phylum was Proteobacteria (47.8 %), followed by Bacteroidetes (18.2 %), Chloroflexi (9.0 %), and Verrucomicrobia (5.8 %) before HPJD treatment. These phyla retained their dominance after HPJD treatment; however, species in the phylum Actinobacteria (6.9-19.9 %) were intensively damaged. A higher degree of sludge destruction by the HPJD increased the species destroyed, but the composition was not changed. The microscopic inspection indicated the HPJD treatment pronouncedly destroyed the eukaryotic metazoa in activated sludge.
  2020年04月15日, 研究論文（学術雑誌）, 共同, 157, 1369-703X, DOI(公開)(r-map)
• Complete Genome Sequence of Pseudomonas putida Strain TS312, Harboring an HdtS-Type N-Acyl-Homoserine Lactone Synthase, Isolated from a Paper Mill
  Hosoe, A., Suenaga, T., Sugi, T., Iizumi, T., Nagai, N., Terada, A.
  Microbiology Resource Announcements
  American Society for Microbiology
  2020年03月26日, 研究論文（学術雑誌）, 共同, 9, 13, DOI(公開)(r-map), e00055-20
• Predicting the acute ecotoxicity of chemical substances by machine learning using graph theory
  Takata, Michiyoshi; Lin, Bin-Le; Xue, Mianqiang; Zushi, Yasuyuki; Terada, Akihiko; Hosomi, Masaaki
  CHEMOSPHERE
  PERGAMON-ELSEVIER SCIENCE LTD
  Accurate in silico predictions of chemical substance ecotoxicity has become an important issue in recent years. Most conventional methods, such as the Ecological Structure-Activity Relationship (ECOSAR) model, cluster chemical substances empirically based on structural information and then predict toxicity by employing a log P linear regression model. Due to empirical classification, the prediction accuracy does not improve even if new ecotoxicity test data are added. In addition, most of the conventional methods are not appropriate for predicting the ecotoxicity on inorganic and/or ionized compounds. Furthermore, a user faces difficulty in handling multiple Quantitative Structure-Activity Relationship (QSAR) formulas with one chemical substance. To overcome the flaws of the conventional methods, in this study a new method was developed that applied unsupervised machine learning and graph theory to predict acute ecotoxicity. The proposed machine learning technique is based on the large AIST-MeRAM ecotoxicity test dataset, a software program developed by the National Institute of Advanced Industry Science and Technology for Multi-purpose Ecological Risk Assessment and Management, and the Molecular ACCess System (MACCS) keys that vectorize a chemical structure to 166-bit binary information. The acute toxicity of fish, daphnids, and algae can be predicted with good accuracy, without requiring log P and linear regression models in existing methods. Results from the new method were cross-validated and compared with ECOSAR predictions and show that the new method provides better accuracy for a wider range of chemical substances, including inorganic and ionized compounds. (C) 2019 Elsevier Ltd. All rights reserved.
  2020年01月01日, 研究論文（学術雑誌）, 共同, 238, 0045-6535, DOI(公開)(r-map), 124604
• アナモックスを用いた窒素除去のいまとこれから
  寺田昭彦
  日本エネルギー学会機関誌えねるみくす
  2020年01月, （MISC）総説・解説（学術雑誌）, 単独, 99, 1, DOI(公開)(r-map), 42, 47
• Inhibition of Agrobacterium tumefaciens biofilm formation by acylase I-immobilized polymer surface grafting of a zwitterionic group-containing polymer brush
  Bao, Qian; Xie, Li; Ohashi, Hidenori; Hosomi, Masaaki; Terada, Akihiko
  BIOCHEMICAL ENGINEERING JOURNAL
  ELSEVIER
  Membrane processes for water purification and wastewater treatment are impacted by biofilm formation, a hotspot for bacterial cell-cell communication, mainly mediated by N-acyl homoserine lactones (AHLs) as signal compounds. Polymer materials for membrane filtration require high water permeability and biofilm deterrents. Therefore, in this study, a hydrophilic and enzyme-immobilizing polymer membrane was designed, for the first time, to prevent biofilm formation by degrading AHLs. A polyethylene membrane (PE) sheet was grafted as a substratum with glycidyl methacrylate (GMA) by radiation-induced graft polymerization (RIGP). The epoxy group in GMA was further converted into dimethylamino-gamma-butyric acid (DMGABA) to increase hydrophilicity. To prevent biofilm formation by cell-cell communication, the quorum-quenching enzyme, acylase I, with or without activity was immobilized onto a DMGABA membrane sheet, providing enzymatically active DMGABA (EI-DMGABA) and inactive DMGABA (Ina-EI-DMGABA) sheets, respectively. The introduction of DMGABA improved hydrophilicity with a static contact angle of 30.2 degrees, as compared to GMA (84.3 degrees). In a flow cell biofilm formation experiment, biofilm formation of Agrobacterium tumefaciens, using AHLs for quorum sensing, was dramatically inhibited on an EI-DMGABA sheet, but not on an Ina-EI-DMGABA sheet. The successful result to prove the concept of active AHL-degrading enzyme immobilized onto a hydrophilic polymer surface likely paves the way for the application to inhibition of unfavorable biofilm formation onto an interface.
  2019年12月15日, 研究論文（学術雑誌）, 共同, 152, 1369-703X, DOI(公開)(r-map), 107372
• Determining uncertainties in PICRUSt analysis - An easy approach for autotrophic nitrogen removal
  Agrawal, Shelesh; Co Thi Kinh; Schwartz, Thomas; Hosomi, Masaaki; Terada, Akihiko; Lackner, Susanne
  BIOCHEMICAL ENGINEERING JOURNAL
  ELSEVIER
  The diversity and dynamics of microorganisms in engineered ecosystems have a high impact on performance and operational stability. Nitrogen removal from wastewater is one example of such complex and dynamic ecosystems. Following the microbial community composition and its functional potential is highly valuable information for optimizing performance. Molecular methods and data analysis tools have become more and more popular in recent years. PICRUSt, a bioinformatics tool to predict the functional potential of a sample from 16S rRNA amplicon sequencing, was tested in the context of autotrophic nitrogen removal for its accuracy. Two experimental studies were extended by qPCR to demonstrate how qPCR can be used to deliver information about the accuracy of PICRUSt predictions. Two main points were discovered: (1) the correlation between qPCR data and PICRUSt predictions depends on the relative abundance of the target gene. With higher abundance, better correlations are achievable; (2) the more genome information available, the stronger the correlation.
  2019年12月15日, 研究論文（学術雑誌）, 共同, 152, 1369-703X, DOI(公開)(r-map), 107328
• 機械学習による金属化合物及び電解質の魚類急性生態毒性予測と学習モデルの解釈
  高田 道義, 林 彬勒, 寺田 昭彦, 細見 正明
  Journal of Computer Aided Chemistry
  日本化学会
  2019年12月, 研究論文（学術雑誌）, 共同, 20, DOI(公開)(r-map), 111, 118
• 機械学習による金属化合物及び電解質の魚類急性生態毒性予測と学習モデルの解釈
  高田 道義, 林 彬勒, 寺田 昭彦, 細見 正明
  Journal of Computer Aided Chemistry
  日本化学会
  2019年12月, 研究論文（学術雑誌）, 共同, 20, DOI(公開)(r-map), 104, 110
• Enrichment, Isolation, and Characterization of High-Affinity N2O-Reducing Bacteria in a Gas-Permeable Membrane Reactor
  Suenaga, Toshikazu; Hori, Tomoyuki; Riya, Shohei; Hosomi, Masaaki; Smets, Barth F.; Terada, Akihiko
  ENVIRONMENTAL SCIENCE & TECHNOLOGY
  AMER CHEMICAL SOC
  The recent discovery of nitrous oxide (N2O)-reducing bacteria suggests a potential biological sink for the potent greenhouse gas N2O. For an application toward N2O mitigation, characterization of more isolates will be required. Here, we describe the successful enrichment and isolation of high-affinity N2O-reducing bacteria using a N2O-fed reactor (N2OFR). Two N(2)OFRs, where N2O was continuously and directly supplied as the sole electron acceptor to a biofilm grown on a gas-permeable membrane, were operated with acetate or a mixture of peptone-based organic substrates as an electron donor. In parallel, a NO3--fed reactor (NO3FR), filled with a nonwoven sheet substratum, was operated using the same inoculum. We hypothesized that supplying N2O vs NO3- would enhance the dominance of distinct N2O-reducing bacteria. Clade II type nosZ bacteria became rapidly enriched over Glade I type nosZ bacteria in the N(2)OFRs, whereas the opposite held in the NO3FR. High-throughput sequencing of 16S rRNA gene amplicons revealed the dominance of Rhodocyclaceae in the N(2)OFRs. Strains of the Azospira and Dechloromonas genera, canonical denitrifiers harboring Glade II type nosZ, were isolated with high frequency from the N(2)OFRs (132 out of 152 isolates). The isolates from the N2OFR demonstrated higher N2O uptake rates (V-max: 4.23 x 10(-3)-1.80 x 10(-2) pmol/h/cell) and lower N2O half-saturation coefficients (K-m,(N2O) : 1.55-2.10 mu M) than a Glade I type nosZ isolate from the NO3FR. Furthermore, the Glade II type nosZ isolates had higher specific growth rates on N2O than nitrite as an electron acceptor. Hence, continuously and exclusively supplying N2O in an N2OFR allows the enrichment and isolation of high-affinity N2O-reducing strains, which may be used as N2O sinks in bioaugmentation efforts.
  2019年10月15日, 研究論文（学術雑誌）, 共同, 53, 20, 0013-936X, DOI(公開)(r-map), 12101, 12112
• Enhancing the dewaterability of anaerobically digested sludge using fibrous materials recovered from primary sludge: demonstration from a field study
  Tochioka, Eiji; Yamashita, Manabu; Usui, Jiro; Miyake, Haruo; Terada, Akihiko; Hosomi, Masaaki
  CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY
  SPRINGER
  Sludge dewatering is an important process for determining the operation cost of sludge disposal. Hence, improving the dewaterability of anaerobically digested sludge containing high water content is of paramount significance. For this challenge, we developed a novel process in which fibrous materials initially collected from a primary sedimentation tank, namely recovered fibers, were used as an auxiliary agent to improve the dewaterability of anaerobically digested sludge. The objective of this study was to investigate the feasibility of using the developed process to improve sludge dewaterability. Three fibrous materials individually recovered from the respective primary sedimentation tanks in different wastewater treatment plants (WWTPs) were used to investigate sludge dewaterability, organic C compositions, and calorific values. The recovered fibers showed comparable compositions irrespective of the WWTP. Six different WWTP sludge samples after anaerobic digestion were continuously supplied to three different dewatering devices, i.e., screw press, centrifuge, and belt press machines, with or without the supply of recovered fibers. Irrespective of the type of a dewatering device, the supply of recovered fibers mixed with the tested sludge samples at a feed ratio of 0.18-0.20g-fibers/g-sludge (dry weight) reduced the amount of polymer flocculant by 13-50%, thereby displaying the superiority of the addition of recovered fibers. Furthermore, at fiber feed ratios of 0.20g-fibers/g-sludge and 0.40g-fibers/g-sludge when the rates of sludge supply and polymer flocculant were kept constant, the corresponding water contents in the dewatered sludge cakes decreased to about 74-76% and 69-72%, respectively. The consistent results regardless of the dewatering device or sludge sample showed the significance of the addition of recovered fibers for improvement in sludge dewaterability. [GRAPHICS]
  2019年07月, 研究論文（学術雑誌）, 共同, 21, 5, 1618-954X, DOI(公開)(r-map), 1131, 1141
• Startup, performance, and microbial communities of an anammox reactor inoculated with indigenous sludge for the treatment of high-salinity and mesophilic underground brine
  Yokota, Nobuyuki; Mineshima, Ryota; Yamaguchi, Hideyuki; Hirase, Tatsuaki; Ishikawa, Hisayoshi; Azuma, Takayuki; Hosomi, Masaaki; Terada, Akihiko
  CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY
  SPRINGER
  Anaerobic ammonium oxidation (anammox) has been implemented as a cost-effective nitrogen removal process in wastewater treatment. To apply the process to saline wastewater treatment at temperatures below 30 degrees C, the effectiveness of marine anammox bacteria has been demonstrated in a pilot-scale reactor. Nevertheless, mesophilic conditions, often found in underground brine containing high NH4+ concentrations, have yet to employ an anammox process. The objective of this study was to enrich anammox bacteria capable of removing nitrogen from underground brine possessing a salinity of 3% and at a temperature over 30 degrees C. To select a promising inoculum, biomass from a brine settling tank in a natural gas plant was subjected to quantifying transcripts of anammox 16S rRNA and hydrazine oxidoreductase (hzo) genes by quantitative reverse transcription polymerase chain reaction. A fixed-bed column anammox reactor was fed with the selected inoculum and operated feeding underground brine mixed with NaNO2 solution at a temperature of 38 degrees C. As a result, a nitrogen removal rate (NRR) of 1.42kg-N/m(3)/day was obtained on day 167. An average NRR of 1.21kg-N/m(3)/day and nitrogen removal efficiency of 88% were maintained for 50days. Amplicon sequencing based on the 16S rRNA revealed that anammox bacteria which are phylogenetically close to Candidatus Kuenenia were successfully enriched in the reactor. These results indicate that nonmarine anammox bacteria can be active and predominant under both high-salinity and mesophilic conditions, making it a likely candidate for effective nitrogen removal from underground and waste brine. [GRAPHICS]
  2019年07月, 研究論文（学術雑誌）, 共同, 21, 5, 1618-954X, DOI(公開)(r-map), 1001, 1011
• 排水処理システムの進展から見る窒素マネジメントの現状と課題
  寺田昭彦
  環境バイオテクノロジー学会誌
  環境バイオテクノロジー学会
  2019年06月, 研究論文（学術雑誌）, 単独, 19, 1, 37, 42
• Comparison of leachate percolation and immersion using different inoculation strategies in thermophilic solid-state anaerobic digestion of pig urine and rice straw
  Meng, Lingyu; Maruo, Koki; Xie, Li; Riya, Shohei; Terada, Akihiko; Hosomi, Masaaki
  BIORESOURCE TECHNOLOGY
  ELSEVIER SCI LTD
  Heterogeneous distribution of substrate and microorganisms and low mass transfer limit methane production dramatically in solid-state anaerobic digestion (SS-AD). To overcome this challenge, this study determined the optimal inoculation strategy (complete premix/slurry application) for reusing solid digestate as inoculum and the optimal leachate circulation method (percolation/immersion) using batch digestion. Initially, percolation and immersion (1 h per 3 days) were compared and the result shows that immersing rice straw into leachate was superior to leachate percolation in methane production. Effect of the immersion period (24, 48 and 72 h) in each circulation cycle on methane production was then evaluated for each inoculation strategy. Methane production increased until the immersion period up to 24 h and then decreased, while the average cumulative methane yield with an immersion period of 24 h was (180 mL/g volatile solids). Slurry application with an immersion period 24 h is recommended as the optimum operating condition.
  2019年04月, 研究論文（学術雑誌）, 共同, 277, 0960-8524, DOI(公開)(r-map), 216, 220
• Lessons from a simple ecological wastewater treatment technology for scientific research and advanced engineering
  Terada, A.
  Clean Technologies and Environmental Policy
  Springer Nature
  2019年04月, 研究論文（学術雑誌）, 単独, 21, 4, DOI(公開)(r-map), 717, 718
• Impact of turning waste on performance and energy balance in thermophilic solid-state anaerobic digestion of agricultural waste
  Meng, Lingyu; Xie, Li; Riya, Shohei; Terada, Akihiko; Hosomi, Masaaki
  WASTE MANAGEMENT
  PERGAMON-ELSEVIER SCIENCE LTD
  Mixing is an important operation in solid-state anaerobic digestion (SS-AD) to improve the mass transfer of the solid phase. This study proposed simple turning by loader in common garage-type digester without commonly used mixer or percolation system (simplified SS-AD). In simplified-SS-AD, turning is conducted in open condition. Thus, oxidation of anaerobic sludge during turning would influence digestion performance. Therefore, in this study, the effect of turning wastes by mixing during digestion on a simplified SS-AD fed with rice straw and pig urine was investigated. Four different mixing frequency levels- no mixing (MO) and mixing once a day (M-1/1), once every 3 days (M-1/3) and once a week (M-1/7)- were conducted. Methane yields of MO, M-1/3 and M-1/7 were comparable with each other. Methane yield and lag period of M-1/1 were approximately 61% and 155% of MO (351.2 mL/g VS and 4.7 days), respectively. Furthermore, the chemical oxygen demand (COD) of acetate accumulated in the digestate of M-1/1 was comparable to the difference in the COD of methane production between M-1/1 and the other treatments. Mixing every day also resulted in a higher oxidation-reduction potential and carbon dioxide content. These findings suggest that methanogenesis was inhibited in M-1/1 by frequent mixing in the atmosphere. Net energy analysis of SS-AD plant operation showed that MO can obtain the highest net energy gain, whereas net energy production of M-1/7 was reduced by rewarming after mixing. Therefore, no mixing is the most effective approach for the proposed simplified process. (C) 2019 Elsevier Ltd. All rights reserved.
  2019年03月15日, 研究論文（学術雑誌）, 共同, 87, 0956-053X, DOI(公開)(r-map), 183, 191
• High-rate partial nitrification of semiconductor wastewater: Implications of online monitoring and microbial community structure
  Song, Kang; Sawayanagi, Kaoru; Numano, Tetsurou; Taniichi, Yuya; Kikuchi, Takashi; Takeda, Tomoyuki; Kanou, Hisanao; Riya, Shohei; Hori, Tomoyuki; Hosomi, Masaaki; Terada, Akihiko
  BIOCHEMICAL ENGINEERING JOURNAL
  ELSEVIER SCIENCE BV
  The aims of this study were to investigate the feasibility of partial nitrification (PN) in a sequencing batch reactor (SBR) fed with real wastewater discharged from the washing process at a semiconductor factory, to evaluate the efficacy of online control for nitrite buildup, and to examine the microbial community composition. To this end, online monitoring of NH4+ and NO3- concentrations in an SBR was implemented to track the extant activities of ammonia-oxidizing (AOB) and nitrite-oxidizing bacteria (NOB). The results showed that the rate of NH4+ oxidation by AOB increased during the first 20 days of the operation, and were maintained as high as a maximum volumetric rate of NH4+ removal of 2.17 kg-N/m(3)/day with an NH4+ removal efficiency of 94.1-99.8%. Successful nitrite buildup (> 99.9%) has been achieved after day 20 when AOB activity was 8.11-times higher than NOB activity. High-throughput sequencing of 16S rRNA genes manifested that the relative abundance of cluster 7 Nitrosomonas species in the family Nitrosomonadaceae increased from 0.2% to 50% over total bacteria. On the other hand, the relative abundance of Nitrospira spp. as the predominant NOB decreased from 1.0% to 0.05%, indicating NOB out-selection during SBR operations. A positive correlation was noted between the abundance of the dominant AOB and NH4+ oxidation rate, suggesting that a simple nitrifier community regulated high-rate PN.
  2019年03月15日, 研究論文（学術雑誌）, 共同, 143, 1369-703X, DOI(公開)(r-map), 34, 40
• Removal and immobilization of heavy metals in contaminated soils by chlorination and thermal treatment on an industrial-scale
  Li, Jining; Hashimoto, Yohey; Riya, Shohei; Terada, Akihiko; Hou, Hong; Shibagaki, Yuichi; Hosomi, Masaaki
  CHEMICAL ENGINEERING JOURNAL
  ELSEVIER SCIENCE SA
  Heavy metals contaminated soils are a potential exposure hazard to the public. This study demonstrated the application of chlorination and thermal treatment for remediation of heavy metals-contaminated soils on a full-industrial scale. Five soil samples were treated at a soil remediation factory equipped with an internally-heated rotary kiln (length: 17 m; diameter: 4 m; rotating speed: 0.60 rpm). The target soil temperature was set at about 950 degrees C. During treatment, calcium chloride was added to facilitate chlorination. The chlorination and thermal treatments effectively removed Pb and Cd and led to the slight removal of Cu and Zn but was not effective for Cr and Ni. Chlorination and thermal treatment also significantly reduced the bioaccessibility of heavy metals in soils, particularly Pb and Cd. The results of sequential extraction revealed that thermal treatment generally decreased the carbonate (C2), Fe/Mn oxide (F3) and organic matter (O4) fractions but tended to increase the residual (R5) fractions of heavy metals, indicating that the chlorination and thermal treatment also favored the immobilization of heavy metals. Overall, the results of this study suggest that remediation of heavy metals-contaminated soils by chlorination and thermal treatment is technologically feasible and that there is great potential for practical application of this method.
  2019年03月01日, 研究論文（学術雑誌）, 共同, 359, 1385-8947, DOI(公開)(r-map), 385, 392
• Spatial and daily variations of nitrous oxide emissions from biological reactors in a full-scale activated sludge anoxic/oxic process
  Jia, Sijing; Chen, Xiaoqiang; Suenaga, Toshikazu; Terada, Akihiko; Ishikawa, Susumu; Nishimura, Fumitake; Ding, Shaolan; Fujiwara, Taku
  JOURNAL OF BIOSCIENCE AND BIOENGINEERING
  SOC BIOSCIENCE BIOENGINEERING JAPAN
  Nitrous oxide (N2O) is an important greenhouse gas that can be emitted from wastewater treatment plants (WWFPs). Such emissions are reportedly process specific and related to operational parameters. This study was conducted to clarify spatial and daily variations of N2O in a full-scale activated sludge anoxic/oxic process that consisted of an anoxic tank and three oxic tanks (oxic-1, oxic-2 and oxic-3), all of which except the final sedimentation tank were fully covered. Higher dissolved N2O (D-N2O) loading and gaseous N2O (G-N2O) emissions were observed for oxic-3 than for the anoxic, oxic-1, and oxic-2 tanks, implying that there was higher N2O production potential via nitrification in the latter stage of the oxic tank. Moreover, the sudden decrease in dissolved oxygen concentration after the peak was found to lead to abrupt production of D-N2O at oxic-3 in the anoxic/oxic process. The increases in AOB amoA, AOB nirK and the following AOB norB gene transcripts at the end of the oxic-2 tank suggested that nitrifier denitrification occurred to produce N2O under low dissolved oxygen conditions when the N2O peak was observed. Additionally, the much lower transcription levels of the two nosZ genes suggested lower N2O consumption. The N2O emission factors ranged from 0.087% to 0.302%, and lower N2O emission factors were observed during summer. (C) 2018, The Society for Biotechnology, Japan. All rights reserved.
  2019年03月, 研究論文（学術雑誌）, 共同, 127, 3, 1389-1723, DOI(公開)(r-map), 333, 339
• Nitrous oxide emissions from biofilm processes for wastewater treatment
  Sabba, Fabrizio; Terada, Akihiko; Wells, George; Smets, Barth F.; Nerenberg, Robert
  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  SPRINGER
  This paper discusses the microbial basis and the latest research on nitrous oxide (N2O) emissions from biofilms processes for wastewater treatment. Conditions that generally promote N2O formation in biofilms include (1) low DO values, or spatial DO transitions from high to low within the biofilm; (2) DO fluctuations within biofilm due to varying bulk DO concentrations or varying substrate concentrations; (3) conditions with high reaction rates, which lead to greater formation of intermediates, e.g., hydroxylamine (NH2OH) and nitrite (NO2-), that promote N2O formation; and (4) electron donor limitation for denitrification. Formation of N2O directly results from the activities of ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA), and heterotrophic denitrifying bacteria. More research is needed on the roles of AOA, comammox, and specialized denitrifying microorganisms. In nitrifying biofilms, higher bulk ammonia (NH3) concentrations, higher nitrite (NO2-) concentrations, lower dissolved oxygen (DO), and greater biofilm thicknesses result in higher N2O emissions. In denitrifying biofilms, N2O accumulates at low levels as an intermediate and at higher levels at the oxic/anoxic transition regions of the biofilms and where COD becomes limiting. N2O formed in the outer regions can be consumed in the inner regions if COD penetrates sufficiently. In membrane-aerated biofilms, where nitrification takes place in the inner, aerobic biofilm region, the exterior anoxic biofilm can serve as a N2O sink. Reactors that include variable aeration or air scouring, such as denitrifying filters, trickling filters, or rotating biological contactors (RBCs), can form peaks of N2O emissions during or following a scouring or aeration event. N2O emissions from biofilm processes depend on the microbial composition, biofilm thickness, substrate concentrations and variability, and reactor type and operation. Given the complexity and difficulty in quantifying many of these factors, it may be difficult to accurately predict emissions for full-scale treatment plants. However, a better understanding of the mechanisms and the impacts of process configurations can help minimize N2O emission from biofilm processes for wastewater treatment.
  2018年11月, 研究論文（学術雑誌）, 共同, 102, 22, 0175-7598, DOI(公開)(r-map), 9815, 9829
• 天然ガスかん水の1槽型部分亜硝酸化-Anammox処理におけるアルカリ使用量の削減
  横田信幸、渡辺泰継、細見正明、寺田昭彦
  化学工学論文集
  2018年09月, 研究論文（学術雑誌）, 共同, 44, 5, DOI(公開)(r-map), 324, 333
• Immobilization of Azospira sp. strain I13 by gel entrapment for mitigation of N2O from biological wastewater treatment plants: Biokinetic characterization and modeling
  T. Suenaga, R. Aoyagi, N. Sakamoto, S. Riya, H. Ohashi, M. Hosomi, H. Tokuyama and A. Terada
  Journal of Bioscience and Bioengineering
  2018年08月, 研究論文（学術雑誌）, 共同, 126, 2, DOI(公開)(r-map), 213, 219
• Investigations of water-extractability of As in excavated urban soils using sequential leaching tests: Effect of testing parameters
  Li, Jining; Kosugi, Tomoya; Riya, Shohei; Hashimoto, Yohey; Hou, Hong; Terada, Akihiko; Hosomi, Masaaki
  JOURNAL OF ENVIRONMENTAL MANAGEMENT
  ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
  Excavated soils with low-level As contamination obtained from construction projects during city development have been of great concern in Japan. Water-extractable As represents the most easily mobilized and ecotoxicologically relevant fraction in the soil environment. In the present study, the water-extractability of As in excavated alkaline urban soils was assessed using sequential leaching tests (SLTs) with a focus on the effects of test parameters. In addition, the potentially water-leachable As over an extremely long period was assessed using the pollution potential leaching index (PPLI), from which one can estimate the number of extractions required to reduce the As in the cumulative leachates to below the Japanese environmental standard (10 mu g L-1). Total As concentrations varied from 6.75 to 79.4 mg kg(-1), and As was continuously detectable among replicate SLT experiments. The water extractable As obtained in the first step of the SLT accounted for 0.41%-7.60% of total As (average: 2.36%), while the cumulative released As in the SLTs corresponded to 1.30%-21.6% of the total (average: 10.6%). The variability of the water-soluble fractions was sensitive to the test conditions. The shaking time at each SLT step had the largest effect on the As water-extractability; followed by sample storage, shaking speed and shaking interruption. A longer shaking time in the standard leaching test of excavated soils is suggested for regulatory purposes in Japan. The use of the PPLI concept for quick estimation of the potential As leachability from excavated soils was supported by the good reproducibility of PPLI results obtained from SLTs under different test parameters. (C) 2018 Elsevier Ltd. All rights reserved.
  2018年07月01日, 研究論文（学術雑誌）, 共同, 217, 0301-4797, DOI(公開)(r-map), 297, 304
• Significance of co-digestion as energy recovery at a sewage treatment plant amenable to a shrinking population
  Terada, Akihiko
  CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY
  SPRINGER
  2018年07月, 研究論文（学術雑誌）, 単独, 20, 5, 1618-954X, DOI(公開)(r-map), 909, 910
• Novel composite gel beads for the immobilization of ammonia-oxidizing bacteria: Fabrication, characterization, and biokinetic analysis
  Aoyagi, Ryo; Sato, Ryuichi; Terada, Akihiko; Tokuyama, Hideaki
  CHEMICAL ENGINEERING JOURNAL
  ELSEVIER SCIENCE SA
  Novel composite gel beads that entrap ammonia-oxidizing bacteria (AOB) were developed for wastewater treatment. The AOB-entrapping composite gel beads, which were monodispersed and millimeter-sized, were produced by a method that combined suspension-gelation with sedimentation polymerization. This method involves the preparation of calcium alginate microcapsules, which contain AOB, by electrostatic atomization and free radical polymerization of droplets of a pre-gel aqueous suspension containing microcapsules during their descent into silicone oil. AOB-entrapping non-porous gel beads, without the microcapsules, were also prepared as a control. The AOB entrapped in both the composite and non-porous gels were grown in a continuous stirred tank reactor fed with synthetic inorganic nitrogenous wastewater. The AOB-entrapping gels successfully catalyzed ammonia oxidation. The kinetics of the reaction were analyzed using the Haldane model with inhibition by a substrate.
  2018年06月15日, 研究論文（学術雑誌）, 共同, 342, 1385-8947, DOI(公開)(r-map), 260, 265
• The influence of the total solid content on the stability of dry-thermophilic anaerobic digestion of rice straw and pig manure
  Riya, Shohei; Suzuki, Kazuhiro; Meng, Lingyu; Zhou, Sheng; Terada, Akihiko; Hosomi, Masaaki
  WASTE MANAGEMENT
  PERGAMON-ELSEVIER SCIENCE LTD
  Dry anaerobic digestion is a promising technology for the recycling of agricultural waste to produce energy and fertilizer. Adding water to the substrate enables better handling and avoid inhibition caused by high total solid (TS) content in the reactor; however, it also increases leachate and operational costs. To assess the extent to which the amount of water added can be reduced, it was examined how the TS content in the reactor influenced the production of biogas. A semi-batch dry thermophilic anaerobic digester was fed with substrate (rice straw and pig manure) at a constant organic loading rate, and varied the TS contents (27%, 32%, 37%, and 42%) of the substrate by adding different amounts of water (representing 0-36% of the total substrate). During incubation, the TS content in the reactor gradually increased from 18% to 31%. Biogas production was stable and high (564 +/- 13-580 +/- 36 N m(3) t(-1) VS), and there was no accumulation of volatile fatty acids when the TS content of the reactor was between 18% and 27%. However, the rate decreased sharply and propionate and acetate were also produced when the TS content of the reactor exceeded 28%. By applying a simple TS balance model, it was found that stable biogas production could be achieved at a substrate TS content of 32%, at which reactor TS content reached 23% at steady-state condition. (C) 2018 Elsevier Ltd. All rights reserved.
  2018年06月, 研究論文（学術雑誌）, 共同, 76, 0956-053X, DOI(公開)(r-map), 350, 356
• Draft genome sequence of Azospira sp. strain I13, a nitrous oxide-reducing bacterium harboring clade II type nosZ
  T. Suenaga, T. Aoyagi, M. Hosomi, T. Hori, A. Terada
  Genome Announcements
  2018年06月, 研究論文（学術雑誌）, 共同, 6, 20, DOI(公開)(r-map), e00414, 18
• Biokinetic Characterization and Activities of N2O-Reducing Bacteria in Response to Various Oxygen Levels
  Suenaga, Toshikazu; Riya, Shohei; Hosomi, Masaaki; Terada, Akihiko
  FRONTIERS IN MICROBIOLOGY
  FRONTIERS MEDIA SA
  Nitrous oxide (N2O)-reducing bacteria, which reduce N2O to nitrogen in the absence of oxygen, are phylogenetically spread throughout various taxa and have a potential role as N2O sinks in the environment. However, research on their physiological traits has been limited. In particular, their activities under microaerophilic and aerobic conditions, which severely inhibit N2O reduction, remain poorly understood. We used an O-2 and N2O micro-respirometric system to compare the N2O reduction kinetics of four strains, i.e., two strains of an Azospira sp., harboring Glade II type nosZ, and Pseudomonas stutzen and Paracoccus denitrificans, harboring Glade I type nosZ, in the presence and absence of oxygen. In the absence of oxygen, the highest N2O-reducing activity, V-m,V-N20, was 5.80 +/- 1.78 x 10(-3) pmol/h/cell of Azospira sp. I13, and the highest and lowest half-saturation constants were 34.8 +/- 10.2 mu M for Pa, denitirificans and 0.866 +/- 0.29 mu M for Azospira sp. I09. Only Azospira sp. I09 showed N2O-reducing activity under microaerophilic conditions at oxygen concentrations below 110 mu M, although the activity was low (10% of V-m,V-N2O). This trait is represented by the higher O-2 inhibition coefficient than those of the other strains. The activation rates of N2O reductase, which describe the resilience of the N2O reduction activity after O-2 exposure, differ for the two strains of Azospira sp. (0.319 +/- 0.028 h(-1) for strain I09 and 0.397 +/- 0.064 h(-1) for strain I13) and Ps. stutyen (0.200 +/- 0.013 h(-1)), suggesting that Azospira sp. has a potential for rapid recovery of N2O reduction and tolerance against O-2 inhibition. These physiological characteristics of Azospira sp. can be of promise for mitigation of N2O emission in industrial applications.
  2018年04月10日, 研究論文（学術雑誌）, 共同, 9, 1664-302X, DOI(公開)(r-map)
• Influence of feedstock-to-inoculum ratio on performance and microbial community succession during solid-state thermophilic anaerobic co-digestion of pig urine and rice straw
  Meng, Lingyu; Xie, Li; Co Thi Kinh; Suenaga, Toshikazu; Hori, Tomoyuki; Riya, Shohei; Terada, Akihiko; Hosomi, Masaaki
  BIORESOURCE TECHNOLOGY
  ELSEVIER SCI LTD
  This study investigated the effect of the feedstock-to-inoculum (F/I) ratio on performance of the solid-state anaerobic co-digestion of pig urine and rice straw inoculated with a solid digestate, and clarified the microbial community succession. A 44-day biochemical methane potential test at F/I ratios of 0.5, 1, 2 and 3 at 55 degrees C and a 35-day large-scale batch test at F/I ratios of 0.5 and 3 at 55 degrees C were conducted to investigate the effects of F/I ratio on anaerobic digestibility and analyze microbial community succession, respectively. The highest cumulative methane yield was 353.7m(3)/t VS in the large-scale batch test. Volatile fatty acids did not accumulate at any F/I ratios. The volatile solids reduction rate was highest at a F/I ratio of 0.5. Microbial community structures were similar between F/I ratios of 3 and 0.5, despite differences in digestion performance, suggesting that stable operation can be achieved at these ratios.
  2018年03月, 研究論文（学術雑誌）, 共同, 252, 0960-8524, DOI(公開)(r-map), 127, 133
• High-rate nitrogen removal from waste brine by marine anammox bacteria in a pilot-scale UASB reactor
  Yokota, Nobuyuki; Watanabe, Yasutsugu; Tokutomi, Takaaki; Kiyokawa, Tomohiro; Hori, Tomoyuki; Ikeda, Daisuke; Song, Kang; Hosomi, Masaaki; Terada, Akihiko
  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  SPRINGER
  The goal of this study was to develop a startup strategy for a high-rate anaerobic ammonium oxidation (anammox) reactor to treat waste brine with high concentrations of ammonium from a natural gas plant. An upflow anaerobic sludge blanket (UASB) anammox reactor with an effective volume of 294 L was fed continuously with waste brine with a salinity of 3% and a NH4 (+) concentration of 180 mg-N/L, as well as a NaNO2 solution. By inoculating a methanogenic granular biomass as a biomass carrier, the reactor attained the maximum volumetric nitrogen removal rate (NRR) of 10.7 kg-N/m(3)/day on day 209, which was 1.7 times higher than the highest reported NRR for wastewater of comparable salinity. High-throughput sequencing of 16S rRNA gene amplicons revealed that Candidatus Scalindua wagneri was enriched successfully in granules in the UASB, and it replaced Methanosaeta and became dominant in the granule. The inhibitory effect of NO2 (-) on the anammox reaction in the granules was assessed by a N-15 tracer method, and the results showed that anammox activity was maintained at 60% after exposure to 300 mg-N/L of NO2 (-) for 24 h. Compared with previous studies of the susceptibilities of Candidatus Brocadia and Candidatus Kuenenia to NO2 (-), the enriched marine anammox bacteria were proven to have comparable or even higher tolerances for high NO2 (-) concentrations after a long exposure.
  2018年02月, 研究論文（学術雑誌）, 共同, 102, 3, 0175-7598, DOI(公開)(r-map), 1501, 1512
• N2O production by denitrification in an urban river: evidence from isotopes, functional genes, and dissolved organic matter
  Thuan, Nguyen Cong; Koba, Keisuke; Yano, Midori; Makabe, Akiko; Kinh, Co Thi; Terada, Akihiko; Toyoda, Sakae; Yoshida, Naohiro; Tanaka, Yotaro; Katsuyama, Masanori; Yoh, Muneoki
  LIMNOLOGY
  SPRINGER JAPAN KK
  Rivers are important sources of N2O emissions into the atmosphere. Nevertheless, N2O production processes in rivers are not well identified. We measured concentrations and isotopic ratios of N2O, NH4 (+), NO2 (-), and NO3 (-) in surface water to identify the microbial processes of N2O production along the Tama River in Japan. We also measured the functional gene abundance of nitrifiers and denitrifiers (amoA-bacteria, nirK, nirS, nosZ clade I, nosZ clade II) together with concentrations of dissolved organic carbon (DOC) and fluorescence intensities of protein and humic components of dissolved organic matter (DOM) to support the elucidation of N2O production processes. The observed nitrogen (delta N-15) and oxygen (delta O-18) of N2O were within the expected isotopic range of N2O produced by nitrate reduction, indicating that N2O was dominantly produced by denitrification. The positive significant correlation between N2ONet concentration and nirK gene abundance implied that nitrifiers and denitrifiers are contributors to N2O production. Fluorescence intensities of protein and humic components of DOM and concentrations of DOC did not show significant correlations with N2O concentrations, which suggests that DOC and abundance of DOM components do not control dissolved N2O. Measurement of isotope ratios of N2O and its substrates was found to be a useful tool to obtain evidence of denitrification as the main source of N2O production along the Tama River.
  2018年01月, 研究論文（学術雑誌）, 共同, 19, 1, 1439-8621, DOI(公開)(r-map), 115, 126
• Pollution potential leaching index as a tool to assess water leaching risk of arsenic in excavated urban soils
  Li, Jining; Kosugi, Tomoya; Riya, Shohei; Hashimoto, Yohey; Hou, Hong; Terada, Akihiko; Hosomi, Masaaki
  ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
  ACADEMIC PRESS INC ELSEVIER SCIENCE
  Leaching of hazardous trace elements from excavated urban soils during construction of cities has received considerable attention in recent years in Japan. A new concept, the pollution potential leaching index (PPLI), was applied to assess the risk of arsenic (As) leaching from excavated soils. Sequential leaching tests (SLT) with two liquid-to-solid (L/S) ratios (10 and 20 L kg(-1)) were conducted to determine the PPLI values, which represent the critical cumulative L/S ratios at which the average As concentrations in the cumulative leachates are reduced to critical values (10 or 5 mu g L-1). Two models (a logarithmic function model and an empirical two-site first-order leaching model) were compared to estimate the PPLI values. The fractionations of As before and after SLT were extracted according to a five-step sequential extraction procedure. Ten alkaline excavated soils were obtained from different construction projects in Japan. Although their total As contents were low (from 6.75 to 79.4 mg kg(-1)), the As leaching was not negligible. Different L/S ratios at each step of the SLT had little influence on the cumulative As release or PPLI values. Experimentally determined PPLI values were in agreement with those from model estimations. A five-step SLT with an L/S of 10 L kg(-1) at each step, combined with a logarithmic function fitting was suggested for the easy estimation of PPLI. Results of the sequential extraction procedure showed that large portions of more labile As fractions (non-specifically and specifically sorbed fractions) were removed during long-term leaching and so were small, but non-negligible, portions of strongly bound As fractions.
  2018年01月, 研究論文（学術雑誌）, 共同, 147, 0147-6513, DOI(公開)(r-map), 72, 79
• Identification of hotspots for NO and N2O production and consumption in counter- and co-diffusion biofilms for simultaneous nitrification and denitrification
  Co Thi Kinh; Riya, Shohei; Hosomi, Masaaki; Terada, Akihiko
  Bioresource Technology
  ELSEVIER SCI LTD
  2017年12月, 研究論文（学術雑誌）, 共同, 245, 0960-8524, DOI(公開)(r-map), 318, 324
• Counter-diffusion biofilms have lower N2O emissions than co-diffusion biofilms during simultaneous nitrification and denitrification: Insights from depth-profile analysis
  Co Thi Kinh; Suenaga, Toshikazu; Hori, Tomoyuki; Riya, Shohei; Hosomi, Masaaki; Smets, Barth F.; Terada, Akihiko
  Water Research
  PERGAMON-ELSEVIER SCIENCE LTD
  2017年11月, 研究論文（学術雑誌）, 共同, 124, 0043-1354, DOI(公開)(r-map), 363, 371
• Efficacy of a high-pressure jet device for excess sludge reduction in a conventional activated sludge process: Pilot-scale demonstration
  Yoshino, Hiroyuki; Suenaga, Toshikazu; Fujii, Tadahiro; Hori, Tomoyuki; Terada, Akihiko; Hosomi, Masaaki
  Chemical Engineering Journal
  ELSEVIER SCIENCE SA
  2017年10月, 研究論文（学術雑誌）, 共同, 326, 1385-8947, DOI(公開)(r-map), 78, 86
• Breaking trade-off in nitrogen management and sustainability
  Terada, Akihiko
  Clean Technologies and Environmental Policy
  SPRINGER
  2017年10月, 研究論文（学術雑誌）, 単独, 19, 8, 1618-954X, DOI(公開)(r-map), 1993, 1994
• Disentangling the multiple effects of a novel high pressure jet device upon bacterial cell disruption
  Xie, Li; Terada, Akihiko; Hosomi, Masaaki
  Chemical Engineering Journal
  ELSEVIER SCIENCE SA
  2017年09月, 研究論文（学術雑誌）, 共同, 323, 1385-8947, DOI(公開)(r-map), 105, 113
• Use of batch leaching tests to quantify arsenic release from excavated urban soils with relatively low levels of arsenic
  Li, Jining; Kosugi, Tomoya; Riya, Shohei; Hashimoto, Yohey; Hou, Hong; Terada, Akihiko; Hosomi, Masaaki
  Journal of Soils and Sediments
  SPRINGER HEIDELBERG
  2017年08月, 研究論文（学術雑誌）, 共同, 17, 8, 1439-0108, DOI(公開)(r-map), 2136, 2143
• 微生物の“棲み分け”を利用した窒素除去技術：バイオフィルムの好気・嫌気部位の有効利用
  寺田昭彦
  分離技術
  2017年08月, 単独, 47, 4, 2, 7
• 河川敷で発生する植物バイオマスの高温乾式メタン発酵特性
  Riya, Shohei; Sawayanagi, Kaoru; Suzuki, Kazuhiro; Zhou, Sheng; Terada, Akihiko; Hosomi, Masaaki
  化学工学論文集
  SOC CHEMICAL ENG JAPAN
  2017年07月, 研究論文（学術雑誌）, 共同, 43, 4, 0386-216X, DOI(公開)(r-map), 224, 230
• Effect of Biological and Mass Transfer Parameter Uncertainty on N2O Emission Estimates from WRRFs
  Song, Kang; Harper, Willie F., Jr.; Takeuchi, Yuki; Hosomi, Masaaki; Terada, Akihiko
  Water Environment Research
  WATER ENVIRONMENT FEDERATION
  2017年07月, 研究論文（学術雑誌）, 共同, 89, 7, 1061-4303, DOI(公開)(r-map), 580, 585
• Nitrosomonas europaeaによるクラリスロマイシンの分解可能性
  Terada, Akihiko; Usui, Hinako; Bao, Qian; Nakai, Satoshi; Hosomi, Masaaki
  化学工学論文集
  SOC CHEMICAL ENG JAPAN
  2017年07月, 研究論文（学術雑誌）, 共同, 43, 4, 0386-216X, DOI(公開)(r-map), 264, 270
• 下水に含まれる繊維状物を用いた嫌気性消化汚泥の脱水性向上
  Tochioka, Eiji; Yamashita, Manabu; Usui, Jiro; Hosokawa, Hisashi; Terada, Akihiko; Hosomi, Masaaki
  化学工学論文集
  SOC CHEMICAL ENG JAPAN
  2017年07月, 研究論文（学術雑誌）, 共同, 43, 4, 0386-216X, DOI(公開)(r-map), 238, 244
• Nitrous oxide production and mRNA expression analysis of nitrifying and denitrifying bacterial genes under floodwater disappearance and fertilizer application
  Riya, Shohei; Takeuchi, Yuki; Zhou, Sheng; Terada, Akihiko; Hosomi, Masaaki
  Environmental Science and Pollution Research
  SPRINGER HEIDELBERG
  2017年06月, 研究論文（学術雑誌）, 共同, 24, 18, 0944-1344, DOI(公開)(r-map), 15852, 15859
• Nitrite oxidation kinetics of two Nitrospira strains: The quest for competition and ecological niche differentiation
  Ushiki, Norisuke; Jinno, Masaru; Fujitani, Hirotsugu; Suenaga, Toshikazu; Terada, Akihiko; Tsuneda, Satoshi
  Journal of Bioscience and Bioengineering
  SOC BIOSCIENCE BIOENGINEERING JAPAN
  2017年05月, 研究論文（学術雑誌）, 共同, 123, 5, 1389-1723, DOI(公開)(r-map), 581, 589
• Synthesis of CTAB intercalated graphene and its application for the adsorption of AR265 and AO7 dyes from water
  Yusuf, Mohammed; Khan, Moonis Ali; Otero, Marta; Abdullah, E. C.; Hosomi, Masaaki; Terada, Akihiko; Riya, Shohei
  Journal of Colloid and Interface Science
  ACADEMIC PRESS INC ELSEVIER SCIENCE
  2017年05月, 研究論文（学術雑誌）, 共同, 493, 0021-9797, DOI(公開)(r-map), 51, 61
• How can we transfer scientific discoveries to engineered systems?: An example of exploring unknown bacteria
  Terada, Akihiko
  Clean Technologies and Environmental Policy
  SPRINGER
  2017年04月, 研究論文（学術雑誌）, 単独, 19, 3, 1618-954X, DOI(公開)(r-map), 625, 626
• Hybrid Nitrous Oxide Production from a Partial Nitrifying Bioreactor: Hydroxylamine Interactions with Nitrite
  Terada, Akihiko; Sugawara, Sho; Hojo, Keisuke; Takeuchi, Yuki; Riya, Shohei; Harper, Willie F., Jr.; Yamamoto, Tomoko; Kuroiwa, Megumi; Isobe, Kazuo; Katsuyama, Chie; Suwa, Yuichi; Koba, Keisuke; Hosomi, Masaaki
  Environmental Science &Technology
  AMER CHEMICAL SOC
  2017年03月, 研究論文（学術雑誌）, 共同, 51, 5, 0013-936X, DOI(公開)(r-map), 2748, 2756
• Antibacterial and anti-biofilm efficacy of fluoropolymer coating by a 2,3,5,6-tetrafluoro-p-phenylenedimethanol structure
  Bao, Qian; Nishimura, Norihito; Kamata, Hirotoshi; Furue, Kentaro; Ono, Yuki; Hosomi, Masaaki; Terada, Akihiko
  Colloids and Surfaces B-Biointerfaces
  ELSEVIER SCIENCE BV
  2017年03月, 研究論文（学術雑誌）, 共同, 151, 0927-7765, DOI(公開)(r-map), 363, 371
• Free nitrous acid and pH determine the predominant ammonia-oxidizing bacteria and amount of N2O in a partial nitrifying reactor
  Kinh, Co Thi; Ahn, Johwan; Suenaga, Toshikazu; Sittivorakulpong, Nakanya; Noophan, Pongsak; Hori, Tomoyuki; Riya, Shohei; Hosomi, Masaaki; Terada, Akihiko
  Applied Microbiology and Biotechnology
  SPRINGER
  2017年02月, 研究論文（学術雑誌）, 共同, 101, 4, 0175-7598, DOI(公開)(r-map), 1673, 1683
• Resuscitation of starved suspended- and attached-growth anaerobic ammonium oxidizing bacteria with and without acetate
  Phanwilai, Supaporn; Wantawin, Chalermraj; Terada, Akihiko; Noophan, Pongsak (Lek); Munakata-Marr, Junko
  Water Science and Technology
  IWA PUBLISHING
  2017年01月, 研究論文（学術雑誌）, 共同, 75, 1, 0273-1223, DOI(公開)(r-map), 115, 127
• 微生物生態と水環境工学に関する最新研究動向
  久保田健吾, 伊藤司，青井議輝，佐藤弘泰，金田一智規，井上謙吾，寺田昭彦，栗栖太
  日本水環境学会誌
  2016年12月, （MISC）総説・解説（学術雑誌）, 共同, 39, 12, 444, 451
• Single-cell analysis of the disruption of bacteria with a high-pressure jet device: an application of atomic force microscopy
  L. Xie, Q. Bao, A. Terada, M. Hosomi
  Chemical Engineering Journal
  2016年12月, 研究論文（学術雑誌）, 共同, 306, DOI(公開)(r-map), 1099, 1108
• Dodecyl sulfate chain anchored mesoporous graphene: Synthesis and application to sequester heavy metal ions from aqueous phase
  M. Yusuf, M. A. Khan, E.C. Abdullah, M. Elfghi, M. Hosomi, A. Terada, S. Riya, A. Ahmad
  Chemical Engineering Journal
  2016年11月, 研究論文（学術雑誌）, 共同, 304, DOI(公開)(r-map), 431, 439
• 次々と明らかになる窒素循環を担う微生物群の多機能・多様性
  寺田昭彦
  化学工学会
  2016年11月, 単独, 11, 757
• Potential for leaching of arsenic from excavated rock after different drying treatments
  J Li, T Kosugi, S Riya, Y Hashimoto, H Hou, A Terada, M Hosomi
  Chemosphere
  2016年11月, 研究論文（学術雑誌）, 共同, 154, DOI(公開)(r-map), 276, 282
• 排水処理生物反応槽におけるN2Oを還元可能な細菌群の利用可能性
  寺田昭彦、末永俊和
  日本微生物生態学会誌
  2016年09月, 共同, 31, 2, 65, 67
• Variation of the microbial community in thermophilic anaerobic digestion of pig manure mixed with different ratios of rice straw
  S. Zhou, M. Nikolausz, J. Zhang, S. Riya, A. Terada, M. Hosomi
  Journal of Bioscience and Bioengineering
  2016年09月, 研究論文（学術雑誌）, 共同, 122, 3, DOI(公開)(r-map), 334, 340
• Predicting the responses of soil nitrite-oxidizers to multi-factorial global change: a trait-based approach
  LE ROUX, X., Bouskill, N., Niboyet, A., Barthes, L., Dijkstra, P., Field, C., Hungate, B.A., Lerondelle, C., Pommier, T., Tang, J., Terada, A., Tourna, M. and Poly
  Frontiers in Microbiology
  2016年05月, 研究論文（学術雑誌）, 共同, 7, DOI(公開)(r-map), 628
• Influence of C/N Ratio on Performance and Microbial Community Structure of Dry-Thermophilic Anaerobic Co-Digestion of Swine Manure and Rice Straw
  S. Riya, K. Suzuki, A. Terada, M. Hosomi, S. Zhou
  Journal of Medical and Bioengineering
  2016年02月, 研究論文（学術雑誌）, 共同, 5, DOI(公開)(r-map)
• Effects of aeration and internal recycle flow on nitrous oxide emissions from a Modified Ludzak-Ettinger process fed with glycerol
  K. Song, T. Suenaga, W. F. Harper Jr., T. Hori, S. Riya, M. Hosomi, A. Terada
  Environmental Science and Pollution Research
  2015年12月, 研究論文（学術雑誌）, 共同, 22, 24, DOI(公開)(r-map), 19562, 19570
• Decomposition of insoluble cyanide in contaminated-soil by based-activated sodium persulfate
  K. Noguchi, K. Fukunaga, E. Imayasu, A. Terada, M. Hosomi
  Journal of Chemical Engineering of Japan
  2015年12月, 研究論文（学術雑誌）, 共同, 48, 12, DOI(公開)(r-map), 970, 975
• Removal of PCBs and HCB from contaminated solids using a novel successive self-propagated sintering process
  L. Zhao, T. Zhu, H. Hou, X. Qin, F. Li, A. Terada, M. Hosomi
  Environmental Science and Pollution Research
  2015年11月, 研究論文（学術雑誌）, 共同, 22, 22, DOI(公開)(r-map), 17527, 17539
• Novel abiotic reactions increase nitrous oxide production during partial nitrification: Modeling and experiments
  W. F. Harper, Y. Takeuchi, S. Riya, M. Hosomi, A. Terada
  Chemical Engineering Journal
  2015年11月, 研究論文（学術雑誌）, 共同, 281, DOI(公開)(r-map), 1017, 1023
• 排水処理システムにおける細菌細胞間コミュニケーション-促進と抑制に向けて-
  寺田昭彦
  化学療法の領域
  2015年10月, 単独, 31, 11, 108, 115
• Successive self-propagating sintering process using carbonaceous materials: A novel low-cost remediation approach for dioxin-contaminated solids
  L. Zhao, H. Hou, T. Zhu, F. Li, A. Terada, M. Hosomi
  Journal of Hazardous Materials
  2015年10月, 研究論文（学術雑誌）, 共同, 299, DOI(公開)(r-map), 231, 240
• Impact of carbon sources on nitrous oxide emission and microbial community structure in an anoxic/oxic activated sludge system
  K. Song, W. F. Harper, T. Hori, S. Riya, M. Hosomi, A. Terada
  Clean Technologies and Environment Policy
  2015年10月, 研究論文（学術雑誌）, 共同, 17, 8, DOI(公開)(r-map), 2375, 2385
• 硝化プロセスにおける亜酸化窒素の放出特性と生成機構
  寺田昭彦
  水環境学会誌
  2015年09月, 単独, 38A, 9, 334, 339
• Effects of N loading rate on CH4 and N2O emissions during cultivation and fallow periods from forage rice fields fertilized with liquid cattle waste
  S. Riya, S. Zhou, Y. Kobara, M. Sagehashi, A. Terada, M. Hosomi
  Journal of Environmental Management
  2015年09月, 研究論文（学術雑誌）, 共同, 161, DOI(公開)(r-map), 124, 130
• Identification of a predominant effect on bacterial cell disruption and released organic matters by a high-pressure jet device
  L. Xie, Q. Bao, T. Suenaga, H. Yoshino, A. Terada, M. Hosomi
  Biochemical Engineering Journal
  2015年09月, 研究論文（学術雑誌）, 共同, 101, DOI(公開)(r-map), 220, 227
• Effect of carbon sources on nitrous oxide emission in a Modified Ludzak Ettinger process
  K. Song, S. Riya, M. Hosomi, A. Terada
  Water Science and Technology
  2015年08月, 研究論文（学術雑誌）, 共同, 72, 4, DOI(公開)(r-map), 572, 578
• High-pressure jet device for activated sludge reduction: Feasibility of sludge solubilization
  T. Suenaga, M. Nishimura, H. Yoshino, H. Kato, M. Nonokuchi, T. Fujii, H. Satoh, A. Terada, M. Hosomi
  Biochemical Engineering Journal
  2015年08月, 研究論文（学術雑誌）, 共同, 100, DOI(公開)(r-map), 1, 8
• 回分式・連続式部分硝化型リアクターの亜硝酸酸化性能と硝化細菌のダイナミクス
  澤柳薫、種部悠未、狩野久直、竹田有之、沼野哲朗、利谷翔平、細見正明、寺田昭彦
  用水と廃水
  2015年06月, 研究論文（学術雑誌）, 共同, 57, 6, 431, 440
• 水・排水処理分野におけるバイオフィルムの形成を抑制する材料の進展
  寺田昭彦、高橋恵理加、片山美怜、細見正明
  環境バイオテクノロジー学会誌
  2015年05月, 共同, 14, 2, 131, 137
• Influence of nitrogen loading and plant nitrogen assimilation on nitrogen leaching and N2O emission in forage rice paddy fields fertilized with liquid cattle waste
  S. Riya, S. Zhou, Y. Kobara, M. Sagehashi, A. Terada and M. Hosomi
  Environmental Science and Pollution Research
  2015年04月, 研究論文（学術雑誌）, 共同, 22, 8, DOI(公開)(r-map), 5762, 5771
• Identification and quantification of bacteria and archaea responsible for ammonia oxidation in different activated sludge of full-scale wastewater treatment plants
  N. Sinthusith, A. Terada, M. Hahn, P. Noophan, J. Munakata-Marr and L. A. Figueroa
  Journal of Environmental Science and Health, Part A
  2015年01月, 研究論文（学術雑誌）, 共同, 50, 2, DOI(公開)(r-map), 169, 175
• Mitigation of CH4 and N2O emissions from a forage rice field fertilized with aerated liquid fraction of cattle slurry by optimizing water management and topdressing
  S. Riya, Y. Muroi, M. Kamimura, S. Zhou, A. Terada, M. Hosomi
  Ecological Engineering
  2015年01月, 研究論文（学術雑誌）, 共同, 75, DOI(公開)(r-map), 24, 32
• Mitigation of greenhouse gas emissions by water management in a forage rice paddy field supplemented using dry-thermophilic anaerobic digestion residue
  S. Riya, M. Katayama, E. Takahashi, S. Zhou, A. Terada, M. Hosomi
  Water, Air, & Soil Pollution
  2014年10月, 研究論文（学術雑誌）, 共同, 225, 9, DOI(公開)(r-map), 1, 13
• Abundance, transcription levels and phylogeny of bacteria capable of nitrous oxide reduction in a municipal wastewater treatment plant
  K. Song, T. Suenaga, A. Hamamoto, K. Satou, S. Riya, M. Hosomi, A. Terada
  Journal of Bioscience and Bioengineering
  2014年10月, 研究論文（学術雑誌）, 共同, 118, 3, DOI(公開)(r-map), 289, 297
• The relationship between anammox and denitrification in the sediment of an inland river
  S. Zhou, S. Borjigin, S. Riya, A. Terada, M. Hosomi
  Science of The Total Environment
  2014年06月, 研究論文（学術雑誌）, 共同, 490, C, DOI(公開)(r-map), 1029, 1036
• Study of penetration behavior of PCB-DNAPL in a sand layer by a column experiment
  N. Okuda, T. Shimizu, M. Muratani, A. Terada, M. Hosomi
  Chemosphere
  2014年06月, 研究論文（学術雑誌）, 共同, 114, DOI(公開)(r-map), 59–68
• Sequentially aerated membrane biofilm reactors for autotrophic nitrogen removal: Microbial community composition and dynamics
  C. Pellicer-Nacher, S. Franck, A. Gulay, M. Ruscalleda, A. Terada, W. A. Al-Soud, M. A. Hansen, S. J. Sorensen, B. F. Smets
  Microbial Biotechnology
  2014年01月, 研究論文（学術雑誌）, 共同, 7, 1, DOI(公開)(r-map), 32, 43
• Degradation of polychlorinated naphthalene by mechanochemical treatment
  Y. Nomura, S. Aono, T. Arino, T. Yamamoto, A. Terada, Y. Noma, M. Hosomi
  Chemosphere
  2013年11月, 研究論文（学術雑誌）, 共同, 93, 11, DOI(公開)(r-map), 2657, 2661
• Applicability of a sequencing batch membrane biofilm reactor for simultaneous nitrogen and phosphorus removal from low C/N ratio wastewater
  A. Terada, J. Ito, S. Matsumoto, S. Tsuneda
  Journal of Water and Environment Technology
  2013年11月, 研究論文（学術雑誌）, 共同, 11, 6, DOI(公開)(r-map), 487, 496
• Physiological characteristics of predominant ammonia-oxidizing bacteria enriched from bioreactors with different influent supply regimes
  A. Terada, S. Sugawara, T. Yamamoto, S. Zhou, K. Koba, M. Hosomi
  Biochemical Engineering Journal
  2013年10月, 研究論文（学術雑誌）, 共同, 79, 15, DOI(公開)(r-map), 153, 161
• Removal of PCDD/Fs from contaminated sediment and released effluent gas by charcoal in a proposed cost-effective thermal treatment process
  L. Zhao, H. Hou, K. Iwasaki, A. Terada, M. Hosomi
  Chemosphere
  2013年08月, 研究論文（学術雑誌）, 共同, 93, 8, DOI(公開)(r-map), 1456, 1463
• An immobilization mechanism for lead in fly ash subjected to mecanochemical treatment
  Y. Nomura, K. Fujiwara, A. Terada, S. Nakai, M. Hosomi
  The International Journal of Environment and Waste Management
  2013年05月, 研究論文（学術雑誌）, 共同, 12, 3, DOI(公開)(r-map), 340, 353
• Autotrophic nitrogen removal in a membrane-aerated biofilm reactor under continuous aeration: A demonstration
  K. R. Gilmore, A. Terada, B. F. Smets, S. Lackner, J. L. Garland, N. G. Love
  Environmental Engineering Science
  2013年01月, 研究論文（学術雑誌）, 共同, 30, 1, DOI(公開)(r-map), 38, 45
• Formation pathways of polychlorinated dibenzofurans (PCDFs) in PCBs-contaminated sediments during thermal desorption process
  L. Zhao, K. Shimoda, A. Terada, M. Hosomi
  Chemosphere
  2012年11月, 研究論文（学術雑誌）, 共同, 88, 11, DOI(公開)(r-map), 1368, 1374
• Utilization of recycled charcoal as a thermal source and adsorbent for the treatment of PCDD/Fs contaminated sediment
  L. Zhao, H. Hou, K. Iwasaki, A. Terada, M. Hosomi
  Journal of Hazardous Materials
  2012年10月, 研究論文（学術雑誌）, 共同, 225-226, DOI(公開)(r-map), 182, 189
• Assessing nitrification and denitrification in a paddy soil with different water dynamics and applied liquid cattle waste using the (15)N isotopic technique
  S. Zhou, Y. Sakiyama, S. Riya, X. Song, A. Terada, M. Hosomi
  Science of the total environment
  2012年08月, 研究論文（学術雑誌）, 共同, 430, DOI(公開)(r-map), 93, 100
• The effect of surface charge property on Escherichia coli initial adhesion and subsequent biofilm formation
  A. Terada, K. Okuyama, M. Nishikawa, S. Tsuneda, M. Hosomi
  Biotechnology and Bioengineering
  2012年05月, 研究論文（学術雑誌）, 共同, 109, 7, DOI(公開)(r-map), 1745, 1754
• ガス透過膜を用いたバイオフィルムリアクターによるアゾ色素・窒素化合物の単一槽内除去
  寺田昭彦, 川島祥子, 西川恵美, 周勝, 細見正明
  用水と廃水
  2012年03月, 研究論文（学術雑誌）, 共同, 54, 3, 213, 222
• CH4 and N2O emissions from different varieties of forage rice (Oryza sativa L.) treating liquid cattle waste
  S. Riya, S. Zhou, Y. Watanabe, M. Sagehashi, A. Terada, M. Hosomi
  Science of the Total Environment
  2012年02月, 研究論文（学術雑誌）, 共同, 419, 1, DOI(公開)(r-map), 178, 186
• Mechcanochemical degradation of gamma-hexachlorocyclohexane by a planetary ball mill in the presence of CaO
  Y. Nomura, K. Fujiwara, A. Terada, S. Nakai, M. Hosomi
  Chemosphere
  2012年01月, 研究論文（学術雑誌）, 共同, 86, 3, DOI(公開)(r-map), 228, 234
• Evaluation of permeation rates of soil fumigrants through plastic films by the cup method
  Y. Kobara, Y. Yogo, A. Terada, M. Hosomi
  Journal of Pesticide Science
  2012年01月, 研究論文（学術雑誌）, 共同, 37, 1, DOI(公開)(r-map), 1, 9
• Adequacy of a simple diffusion model to predict benzene behavior in soil
  P. Du, M. Sagehashi, A. Terada, S. Zhou, F.S. Li, M. Hosomi
  Soil Science Society of America Journal
  2011年12月, 研究論文（学術雑誌）, 共同, 75, 6, DOI(公開)(r-map), 2147, 2157
• Presence and detection of anaerobic ammonium-oxidizing (anammox) bacteria and appraisal of anammox process for high-strength nitrogenous wastewater treatment: A review
  A. Terada, S. Zhou, M. Hosomi
  Clean Technologies and Environmental Policy
  2011年12月, 研究論文（学術雑誌）, 共同, 13, 6, DOI(公開)(r-map), 759, 781
• 飯野洸, 周勝, 下ヶ橋雅樹, 中島田豊, 大川泰一郎, 寺田昭彦, 細見正明
  飼料イネの高温メタン発酵特性
  環境科学会誌
  2011年10月, 研究論文（学術雑誌）, 共同, 24, 5, 462, 471
• Structure and activity of lacustrine sediment bacteria involved in nutrient and iron cycles
  G. Martins, A. Terada, D. Ribeiro, A. Brito, B. F. Smets, R. Nogueira
  FEMS Microbiology Ecology
  2011年09月, 研究論文（学術雑誌）, 共同, 77, 3, DOI(公開)(r-map), 666, 679
• Effect of infiltration rate on nitrogen dynamics in paddy soil after high-load nitrogen application containing 15N tracer
  S. Zhou, S. Sugawara, S. Riya, M. Sagehashi, K. Toyota, A. Terada, M. Hosomi
  Ecological Engineering
  2011年07月, 研究論文（学術雑誌）, 共同, 37, 5, DOI(公開)(r-map), 685, 692
• What young water professionals expect from the water sector
  R. Henderson, C. Delfieux, A. Puigarnau, A. Terada, T. Barnard
  Water Utility Management International
  2011年04月, 共同, 3, 31, 32
• Enhancing nitrogen and phosphorus removal in an anaerobic/oxic/anoxic sequencing batch reactor: The dependence of the amount of external carbon
  K. Soejima, A. Terada, K. Naraki, S. Tsuneda
  Journal of Water and Environment Technology
  2011年03月, 研究論文（学術雑誌）, 共同, 9, 1, DOI(公開)(r-map), 79, 86
• Characterization of benzene emission to the atmosphere from contaminated soils
  P. Du, M. Sagehashi, A. Terada, M. Hosomi
  環境科学会誌
  2010年11月, 研究論文（学術雑誌）, 共同, 23, 6, 467, 475
• Diffusion and adsorption of benzene in contaminated Andosol and sand
  P. Du, M. Sagehashi, A. Terada, M. Hosomi
  環境科学会誌
  2010年11月, 研究論文（学術雑誌）, 共同, 23, 6, 459, 466
• Nitritation performance in membrane aerated biofilm reactors differs from conventional biofilm systems
  S. Lackner, A. Terada, H. Horn, M. Henze, B. F. Smets
  Water Research
  2010年11月, 研究論文（学術雑誌）, 共同, 44, 20, DOI(公開)(r-map), 6073, 6084
• Sequential aeration of membrane-aerated biofilm ractors (MABRs) for high-rate autotrophc nitrogen removal: Experimental demonstration
  C. Pellicer-Nacher, S. P. Sun, S. Lackner, A. Terada, F. Schreiber, Q. Zhou, B. F. Smets
  Environmental Science and Technology
  2010年10月, 研究論文（学術雑誌）, 共同, 44, 19, DOI(公開)(r-map), 7628, 7634
• Inoculum effects on community composition and nitritation performance of autotrophic nitrifying biofilm reactors with counter-diffusion geometry
  A. Terada, S. Lackner, K. Kristensen, B. F. Smets
  Environmental Microbiology
  2010年10月, 研究論文（学術雑誌）, 共同, 12, 10, DOI(公開)(r-map), 2858, 2872
• 浸透流式飼料イネ人工湿地における温室効果ガスの 直接および間接排出
  利谷翔平、周勝、中島田豊、寺田昭彦、細見正明
  化学工学論文集
  2010年07月, 研究論文（学術雑誌）, 単独, 36, 4, DOI(公開)(r-map), 229, 236
• Prevention of lead leaching from fly ashes by mechanochemical treatment
  Y. Nomura, K. Fujiwara, A. Terada, S. Nakai, M. Hosomi
  Waste Management
  2010年07月, 研究論文（学術雑誌）, 共同, 30, 7, DOI(公開)(r-map), 1290, 1295
• Behavior of PCDDs/PCDFs in remediation of PCBs-contaminated sediments by thermal desorption
  T. Sato, T. Tomohiro, K. Shimoda, A. Terada, M. Hosomi
  Chemosphere
  2010年06月, 研究論文（学術雑誌）, 共同, 80, 2, DOI(公開)(r-map), 184, 189
• 単一槽型窒素除去を志向したバイオフィルムリアクターの進展
  寺田昭彦、常田聡
  水環境学会誌
  2010年04月, 共同, 33A, 4, 114, 120
• Shifts between Nitrospira- and Nitrobacter-like nitrite oxidizers underly the response of soil potential nitrite oxidation to changes in tillage practices
  E. Attard, F. Poly, F. Laurent, C. Commeaux, A. Terada, B. F. Smets, S. Recous, X. Le Roux
  Environmental Microbiology
  2010年02月, 研究論文（学術雑誌）, 共同, 12, 2, DOI(公開)(r-map), 315, 326
• Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and anammox
  S. E. Vlaeminck, A. Terada, B. F. Smets, H. De Clippeleir, T. Schaubroeck, S. Bolca, L. Demeestere, J. Mast, N. Boon, M. Carballa, W. Verstraete
  Applied and Environmental Microbiology
  2010年01月, 研究論文（学術雑誌）, 共同, 76, 3, DOI(公開)(r-map), 900, 909
• Microbial community structure in autotrophic nitrifying granules characterized by experimental and simulation analyses
  S. Matsumoto, M. Katoku, G. Saeki, A. Terada, Y. Aoi, S. Tsuneda, C. Picioreanu, M.C.M. van Loosdrecht
  Environmental Microbiology
  2010年01月, 研究論文（学術雑誌）, 共同, 12, 1, DOI(公開)(r-map), 192, 206
• Fibrous support stabilizes nitrification performance of a membrane-aerated biofilm: The effect of liquid flow perturbation on process performance
  A. Terada, J. Ito, S. Matsumoto, S. Tsuneda
  Journal of Chemical Engineering of Japan
  2009年08月, 研究論文（学術雑誌）, 共同, 42, 8, DOI(公開)(r-map), 607, 615
• Enhancing the formation and shear resistance of nitrifying biofilms on membranes by surface functionalization
  S. Lackner, M. Holmberg, A. Terada, P Kingshott, B.F. Smets
  Water Research
  2009年08月, 研究論文（学術雑誌）, 共同, 43, DOI(公開)(r-map), 3469, 3478
• Nitrogen removal from digested black water by one-stage partial nitritation and anammox
  S. E. Vlaeminck, A. Terada, B. F. Smets, D. van der Linden, W. Verstraete, M. Carballa
  Environmental Science and Technology
  2009年08月, 研究論文（学術雑誌）, 共同, 43, DOI(公開)(r-map), 5035, 5041
• Nitritation performance and biofilm development of co- and counter-diffusion biofilm reactors: modeling and experimental comparison
  R.C. Wang, A. Terada, S. Lackner, B.F. Smets, M. Henze, S. Xia, J. Zhao
  Water Research
  2009年06月, 研究論文（学術雑誌）, 共同, 43, DOI(公開)(r-map), 2699, 2709
• The effect of hydroxylamine on the activity and aggregate structure of autotrophic nitrifying bioreactor cultures
  W. F. Harper Jr., A. Terada, F. Poly, X. Le Roux, K. Kristensen, M. Mazher, B.F. Smets
  Biotechnology and Bioengineering
  2009年02月, 研究論文（学術雑誌）, 共同, 102, 3, DOI(公開)(r-map), 714, 724
• Modeling and experimental study on the anaerobic/oxic/anoxic process for nitrogen and phosphorus removal: The effect of acetate addition
  K. Soejima, S. Matsumoto, S. Ohgushi, K. Naraki, A. Terada, S. Tsuneda, A. Hirata
  Process Biochemistry
  2008年06月, 研究論文（学術雑誌）, 共同, 43, 6, DOI(公開)(r-map), 605, 614
• Heterotrophic activity compromises autotrophic nitrogen removal in membrane aerated biofilms: a modeling study
  S. Lackner, A. Terada, B.F. Smets
  Water Research
  2008年03月, 研究論文（学術雑誌）, 共同, 42, 3, DOI(公開)(r-map), 1102, 1112
• 微生物固定化のための膜の改質と水処理分野への適用
  常田聡，寺田昭彦
  膜
  2008年03月, 共同, 33, 2, 54, 62
• Modeling of membrane-aerated biofilm: Effect of C/N ratio, biofilm thickness and surface loading of oxygen on feasibility of simultaneous nitrification and denitrification
  S. Matsumoto, A. Terada, S. Tsuneda
  Biochemical Engineering Journal
  2007年10月, 研究論文（学術雑誌）, 共同, 37, 10, DOI(公開)(r-map), 98, 107
• Experimental and simulation analysis of community structure of nitrifying bacteria in a membrane-aerated biofilm
  S. Matsumoto, A. Terada, Y. Aoi, S. Tsuneda, C. Picioreanu, M.C.M. van Loosdrecht
  Water Science and Technology
  2007年08月, 研究論文（学術雑誌）, 共同, 55, 8-9, DOI(公開)(r-map), 283, 290
• 電気泳動による微生物固定化空間内の基質輸送の促進と脱窒反応の向上
  常田聡，賀來周一，林浩志，大串聡，寺田昭彦，平田彰
  化学工学論文集
  2007年06月, 研究論文（学術雑誌）, 共同, 32, 6, DOI(公開)(r-map), 507, 513
• Controlling population dynamics and nitrogen removal performance in hollow fiber membrane-aerated biofilm reactors
  K. R. Gilmore, A. Terada, B. F. Smets, N. G. Love
  IWA Specialist group on Activated Sludge Population Dynamics (ASPD) Newsletter
  2007年05月, 研究論文（学術雑誌）, 共同, 12, 18
• Redox-stratification controlled biofilm (ReSCoBi) for completely autotrophic nitrogen removal: The effect of co- versus counter-diffusion on reactor performance
  A. Terada, S. Lackner, S. Tsuneda, B.F. Smets
  Biotechnology and Bioengineering
  2007年05月, 研究論文（学術雑誌）, 共同, 97, 1, DOI(公開)(r-map), 40, 51
• Bacterial adhesion to and viability on positively charged polymer surfaces
  A. Terada, A. Yuasa, T. Kushimoto, S. Tsuneda, A. Katakai, M. Tamada
  Microbiology-SGM
  2006年12月, 研究論文（学術雑誌）, 共同, 152, 12, DOI(公開)(r-map), 3575, 3583
• Rapid autohydrogenotrophic denitrification by a membrane biofilm reactor equipped with a fibrous support around a gas-permeable membrane
  A. Terada, S. Kaku, S. Matsumoto, S. Tsuneda
  Biochemical Engineering Journal
  2006年06月, 研究論文（学術雑誌）, 共同, 31, 1, DOI(公開)(r-map), 84, 91
• Effects of composition and injective conditions of additional substances on nutrient removal efficiency of the anaerobic/aerobic/anoxic process
  K. Soejima, K. Oki, A. Terada, S. Tsuneda
  Bioprocess and Biosystems Engineering
  2006年05月, 研究論文（学術雑誌）, 共同, 29, 5-6, DOI(公開)(r-map), 305, 313
• Sequencing batch membrane biofilm reactor for simultaneous nitrogen and phosphorus removal: Novel application of membrane-aerated biofilms
  A. Terada, T. Yamamoto, S. Tsuneda, A. Hirata
  Biotechnology and Bioengineering
  2006年04月, 研究論文（学術雑誌）, 共同, 94, 4, DOI(公開)(r-map), 730, 739
• Feasibility of a membrane aerated biofilm reactor to achieve controllable nitrification
  A. Terada, T. Yamamoto, R. Igarashi, S. Tsuneda, A. Hirata
  Biochemical Engineering Journal
  2006年01月, 研究論文（学術雑誌）, 共同, 28, 2, DOI(公開)(r-map), 123, 130
• Comparison of spatial organization in top-down- and membrane-aerated biofilms: A numerical study
  A. Bell, Y. Aoi, A. Terada, S. Tsuneda, A. Hirata
  Water Science and Technology
  2005年07月, 研究論文（学術雑誌）, 共同, 52, 7, DOI(公開)(r-map), 173, 180
• Elucidation of dominant effect on initial bacterial adhesion onto polymer surfaces prepared by radiation-induced graft polymerization
  A. Terada, A. Yuasa, S. Tsuneda, A. Hirata, A. Katakai, M. Tamada
  Colloids and Surfaces B: Biointerfaces
  2005年02月, 研究論文（学術雑誌）, 共同, 43, 2, DOI(公開)(r-map), 99, 107
• Enhancement of biofilm formation onto surface-modified hollow-fiber membranes and its application to membrane-aerated biofilm reactor
  A. Terada, T. Yamamoto, K. Hibiya, S. Tsuneda, A. Hirata
  Water Science and Technology
  2004年11月, 研究論文（学術雑誌）, 共同, 49, 11-12, 263, 268
• メンブレンエアレーション法を応用した単一槽内有機物・窒素同時除去システム
  寺田昭彦，日比谷和明，常田聡，平田彰
  用水と廃水
  2004年04月, 研究論文（学術雑誌）, 共同, 46, 2, 148, 155
• 中空糸メンブレンを用いた新しい水処理技術
  寺田昭彦，日比谷和明，常田聡，平田彰
  水処理技術
  2003年04月, 共同, 44, 4, 153, 164
• Nitrogen removal characteristics and biofilm analysis of a membrane-aerated biofilm reactor applicable to high-strength nitrogenous wastewater treatment
  A. Terada, K. Hibiya, J. Nagai, S. Tsuneda, A. Hirata
  Journal of Bioscience and Bioengineering
  2003年02月, 研究論文（学術雑誌）, 共同, 95, 2, DOI(公開)(r-map), 170, 178
• Simultaneous nitrification and denitrification by controlling vertical and horizontal microenvironment in a membrane-aerated biofilm reactor
  K. Hibiya, A. Terada, S. Tsuneda, A. Hirata
  Journal of Biotechnology
  2003年01月, 研究論文（学術雑誌）, 共同, 100, 1, DOI(公開)(r-map), 23, 32

著書

• 図説窒素と環境の科学 : 人と自然のつながりと持続可能な窒素利用
  林 健太郎, 柴田 英昭, 梅澤 有
  2-7 廃棄物・下水、コラム９ 最先端下水処理技術
  朝倉書店
  2021年12月, 9784254180572
• 水環境の事典
  寺田昭彦
  II-4-3-5 生物学的窒素除去法：硝化・脱窒プロセス
  朝倉書店
  2021年04月05日, 978-4-254-18056-5
• ファウリングの原因と対策・抑制技術
  寺田昭彦
  S&T出版
  2016年02月
• Proteobacteria: Phylogeny, Metabolic Diversity and Ecological Effects
  G. Martinsa, D. C. Ribeiro, A. Terada, B. F. Smets, A. G. Brito, R. Nogueira
  Nova Science Publishers
  2011年01月, 978-1-61122-150-3

研究発表、招待講演等

• ガス透過膜を用いた集積培養装置の亜酸化窒素還元細菌群の集積化培養への利用
  化学工学会第89年会
  2024年03月20日, 口頭発表（招待・特別）
• 膜通気型バイオフィルムリアクターの有機物・窒素除去性能におけるバイオフィルム剥離強度の及ぼす効果
  化学工学会第89年会
  2024年03月18日, ポスター発表
• 高濃度窒素排水からのアンモニア変換・保持に向けた好気性グラニュール法のシミュレーション：粒径と溶存酸素濃度が性能に及ぼす影響
  第58回日本水環境学会年会
  2024年03月07日, ポスター発表
• 省エネ型窒素除去とN2O排出削減に向けた無曝気型散水ろ床法に生息する硝化細菌の生理生態解析
  第58回日本水環境学会年会
  2024年03月06日, 口頭発表（一般）
• 発酵廃水からのアンモニア回収に向けた微好気活性汚泥法の運転状況の確立：低pHが汚泥性状、処理性能、微生物群集構造に及ぼす影響
  第58回日本水環境学会年会
  2024年03月06日, 口頭発表（一般）
• 完全脱窒型のclade II nosZ N 2O還元細菌は排水処理における有力なN2Oシンクとなり得るか？
  日本微生物生態学会第36回大会
  2023年11月30日, 口頭発表（招待・特別）
• 部分硝化リアクターから単離したクラスター7アンモニア酸化細菌の亜酸化窒素生成ダイナミクス：ゲノムおよび生理学的特性からの考察
  日本微生物生態学会第36回大会
  2023年11月29日, ポスター発表
• Insights into microbial nitrogen metabolisms of microaerophilic activated sludge for retaining ammonia from high-strength nitrogenous wastewater: A metagenomic and metatranscriptomic approach
  10th IWA Microbial Ecology and Water Engineering Specialist Conference
  2023年09月12日, 口頭発表（一般）
• Quantitative evaluation of nitrous oxide sink capacity of denitrifying bacteria by a newly developed dual-tracer method
  The Eighth International Conference on Nitrification and Related Processes
  2023年07月31日, ポスター発表
• Genomic and physiological insights into N2O emissions by cluster 7 ammonia-oxidizing bacteria isolated from partial nitrifying bioreactors
  The Eighth International Conference on Nitrification and Related Processes
  2023年07月31日, ポスター発表
• Phylogeny, abundance, and function of N2O-reducing bacteria in an anammox biofilm
  The Eighth International Conference on Nitrification and Related Processes
  2023年07月31日, 口頭発表（一般）
• Effect of Calcium Alginate Gel Concentration on Mitigation of N2O Emission by Gel-Entrapped Azospira sp. strain I13: A Biokinetic Study
  WET2023 Online
  2023年07月08日, ポスター発表
• Organic carbon species selects predominant N2O-reducing bacteria: The insights from biokinetics and genomics of canonical denitrifying bacteria
  18th IWA Leading Edge Conference on Water and Wastewater Technologies
  2023年05月31日, 口頭発表（一般）
• 新規ガス透過膜を用いた膜通気型バイオフィルムリアクターによる排水処理プロセスの開発
  第57回日本水環境学会年会
  2023年03月15日, ポスター発表
• 葉酸添加がAnammox活性に及ぼす影響の15Nトレーサー法による検証
  第57回日本水環境学会年会
  2023年03月15日, ポスター発表
• 有機物除去・アンモニア回収を目指した微好気性グラニュール法の開発
  第57回日本水環境学会年会
  2023年03月15日, ポスター発表
• 異なる脱窒酵素を有するアンモニア酸化細菌のN2O生成ポテンシャルの評価
  第57回日本水環境学会年会
  2023年03月15日, ポスター発表
• Rapid granulation of a microaerophilic mixed suspended culture for organic carbon removal and ammonia retention from food industry wastewater
  第57回日本水環境学会年会
  2023年03月15日, 口頭発表（一般）
• Anammoxプロセスの温室効果ガス削減に貢献するN2O還元細菌の探索と機能解明
  第57回日本水環境学会年会
  2023年03月15日, ポスター発表
• 農業集落排水を用いたハイブリット型MABR 処理評価
  第57回日本水環境学会年会
  2023年03月15日, 口頭発表（一般）
• 乾式メタン発酵残渣炭化物による栄養塩の吸着特性評価
  化学工学会第88年会
  2023年03月15日, ポスター発表
• 乾式メタン発酵における原生動物の存在と役割の解明
  化学工学会第88年会
  2023年03月15日, ポスター発表
• メタン酸化細菌による微生物タンパク質の産生:イネ根圏と土壌からの集積培養と性能評価
  化学工学会第88年会
  2023年03月15日, ポスター発表
• 代替敷料と牛ふん尿の混合メタン発酵特性の解明
  化学工学会第88年会
  2023年03月15日, ポスター発表
• 微好気活性汚泥法を用いた高強度窒素含有排水からのアンモニア回収運転手法の開発
  化学工学会第88年会
  2023年03月15日, ポスター発表
• Spatial distributions of transcriptionally active nitrous oxide-producing/reducing bacteria and microbial communities in a membrane-aerated biofilm reactor
  IWA BIOFILMS CONFERENCE 2022
  2022年12月07日, 口頭発表（一般）
• Hybrid Membrane-Aerated Biofilm Reactor (H-MABR) for Nitrogen Removal from a Mixture of Municipal and Agricultural Wastewater: Performances and Nitrifiers Communities
  IWA BIOFILMS CONFERENCE 2022
  2022年12月07日, 口頭発表（一般）
• Physiological traits of N2O-reducing bacteria in a chemostat bioreactor toward N2O sinks
  日本微生物生態学会 第35回大会
  2022年10月31日, 口頭発表（一般）
• 新規な多重標識トレーサー法を用いたアナモックスリアクターからの N2O放出メカニズムの解明
  日本微生物生態学会 第35回大会
  2022年10月31日, ポスター発表
• Harnessing microbial communities for bioenergy recovery;greenhouse gas mitigations
  2022 The 6th International Conference on Integrated and Innovative Solutions for Circular Ecolony
  2022年10月05日, 口頭発表（招待・特別）
• A15N tracer method reveals canonical denitrifiers, Azospira spp., as a nitrous oxide consumer in the presence of nitrate
  18th International Symposium on Microbial Ecology
  2022年08月12日, ポスター発表
• ガス透過膜を用いた集積培養技術による高活性N2O還元細菌群の集積化と活性評価
  第56回日本水環境学会年会
  2022年03月16日, ポスター発表
• Growth optimization and biokinetic characterization of a novel bioplastic-producing, methane-oxidizing bacterium, Methylosinus sp. C49
  化学工学会第87年会
  2022年03月16日, ポスター発表
• アンモニア酸化細菌によるアンモニア酸化能力と亜酸化窒素排出特性の比較
  化学工学会第87年会
  2022年03月16日, ポスター発表
• 化学工学会第87年会
  2022年03月16日, ポスター発表
• 好塩酸メタン酸化細菌Methylomicrobiumを用いたエクトインの生成条件の把握
  化学工学会年会第87年会
  2022年03月16日, ポスター発表
• 土壌水銀揮発ポテンシャル評価法の開発
  化学工学会第87年会
  2022年03月16日, ポスター発表
• 炭化物を用いた稲わらと豚糞尿乾式メタン発酵におけるアンモニア阻害の緩和
  化学工学会第87年会
  2022年03月16日, ポスター発表
• A high pressure jet device for excess sludge reduction from municipal wastewater treatment plants  
  The 6th EnvironmentAsia Virtual International Conference
  2021年12月21日, 口頭発表（基調）
• Exploring and harnessing N2O-reducing bacteria effectively functioning as sink in an engineered system: The combination of microsensors and microbiological methods
  Webinar at the University of Oulu Assessment of microbial communities and their functions using microsensors and visualization techniques
  2021年11月19日, 公開講演，セミナー，チュートリアル，講習，講義等
• 生活排水処理施設放流水が河川底泥の亜酸化窒素生成と消費活性へ及ぼす影響
  日本微生物生態学会 第34 回大会
  2021年10月31日, ポスター発表
• 15Nトレーサー法を用いた脱窒細菌のN2O還元ポテンシャルの評価
  第24回日本水環境学会シンポジウム
  2021年09月15日, ポスター発表
• 排水処理システムから発生する温室効果ガスN2O：削減に向けた技術開発に向けて
  第２回水再生・バイオソリッド研究会
  2021年08月26日, 公開講演，セミナー，チュートリアル，講習，講義等
• Effect of Treated Water from a Municipal Wastewater Treatment Plant on Nitrous Oxide Production and Consumption Activities of the Downstream River Sediments
  WET2021 Online
  2021年08月11日, ポスター発表
• Genomics and physiology of novel Nitrobacter strain adapted to an acidic soil environment of heavily fertilized tea field
  the 7th International Conference on Nitrification and Related Processes (ICoN7)
  2021年07月, ポスター発表
• Exploring and harnessing N2O-reducing bacteria in an engineered system
  Expert Webinar at Unisense
  2021年06月01日, 公開講演，セミナー，チュートリアル，講習，講義等
• 排水からのNH3回収の安定化に関わるN2O放出ダイナミクスの解明
  化学工学会第86年会
  2021年03月20日, ポスター発表
• 水田土壌の乾燥・湿潤で発生するN2Oの生成反応の解明
  化学工学会第86年会
  2021年03月20日, ポスター発表
• 亜硝酸酸化細菌Nitrobacterの新株が持つ固有の窒素代謝経路と亜酸化窒素還元機能の発見
  第15回日本ゲノム微生物学会年会
  2021年03月04日, ポスター発表
• A membrane-aerated biofilm reactor for efficient single-stage nitrogen removal and reduction of low nitrous oxide emissions
  MABR Webinar at Pusan National University
  2021年01月26日, 公開講演，セミナー，チュートリアル，講習，講義等
• Excess sludge reduction in an activated sludge system installing a high-pressure jet device
  India-Japan Cooperative Programme Development of Sustainable Environment/Energy Technologies in South India
  2019年12月17日, 口頭発表（招待・特別）
• A Membrane-aerated Biofilm Reactor for Effective Nitrogen Removal from Wastewaters: Opportunities and Challenges
  IEEC & BWR 2019
  2019年12月13日, 口頭発表（基調）
• Phylogeny and physiology of nitrous oxide-reducing bacteria in nitrogen-removing bioreactors
  Deammonification and Environmental Technology and Management
  2019年12月11日, 口頭発表（基調）
• Can we harness nitrous oxide-reducing bacteria in nitrogen removing reactors for mitigation? – Potentials and challenges
  The 1st Japan-Korea Joint Symposium on Water Environment for Young Researchers ―New Developments for management of water environment in Asia―
  2019年10月28日, 口頭発表（招待・特別）
• Phylogeny, physiological traits, and activity of nitrous oxide-producing and -reducing bacteria in wastewater bioreactors
  6th International Conference on Nitrification and Related processes
  2019年10月10日, 口頭発表（招待・特別）
• Counter-current substrate diffusion biofilms for nitrogen removal from wastewaters
  The 2nd International Conference on Non-point Source Pollution Control and Aquatic Ecosystem Protection
  2019年09月21日, 口頭発表（招待・特別）
• A membrane-aerated biofilm reactor for cost-effective nitrogen removal with low greenhouse gas emissions: Insights from macro and micro scale observations
  EnvironmentAsia
  2019年06月13日, 口頭発表（招待・特別）
• 亜酸化窒素排出削減に向けた亜酸化窒素還元細菌の探索・動力学的解析
  第92回日本細菌学会
  2019年04月25日, 口頭発表（招待・特別）
• Kinetics, Activity, and Application of N2O-Reducing Bacteria for Mitigation of N2O emissions during Wastewater Treatment
  2019 International Meeting of the Microbiological Society of Korea
  2019年04月18日, 口頭発表（招待・特別）
• A membrane-aerated biofilm reactor for efficient single-stage nitrogen removal and mitigation of nitrous oxide emission: Proof-of-concept from biofilm depth profile analysis
  IWA Water Reuse
  2018年10月31日, 口頭発表（一般）
• ［依頼講演］対抗拡散型バイオフィルムによる水処理技術：窒素除去の観点から見た利点と課題
  化学工学会第50回秋季大会
  2018年09月19日, 口頭発表（招待・特別）
• ［招待講演］工学システムの進展から見る窒素マネジメントの現状と課題
  第70回日本生物工学会大会
  2018年09月07日, 口頭発表（招待・特別）
• 繊維状担体を充填した膜分離活性汚泥法：ファウリング抑制と微生物叢との関連
  第21 回日本水環境学会シンポジウム
  2018年09月05日, 口頭発表（一般）
• 高圧ジェット装置を用いた活性汚泥システムからの余剰汚泥削減：作用機構とその応用
  第7回排水処理技術セミナー
  2018年07月26日, 口頭発表（招待・特別）
• [Keynote] Dry-thermophilic anaerobic co-digestion of rice straw and swine manure:The nexus of wastewater treatment, energy recovery and food production.
  2018 Jade Mountain Forum
  2018年06月22日, 口頭発表（基調）
• 低含水率馴養した種汚泥を用いた豚尿と稲わらの高温乾式メタン発酵
  第52回日本水環境学会年会（2017年度）
  2018年03月17日, 口頭発表（一般）
• 流動する固定床の実槽実験-2
  第52回日本水環境学会年会（2017年度）
  2018年03月17日, 口頭発表（一般）
• 包括固定化担体を添加した部分亜硝酸化リアクターの亜硝酸化性能と微生物群集構造の関係
  第52回日本水環境学会年会（2017年度）
  2018年03月17日, 口頭発表（一般）
• 包括固定化担体を用いた低温低濃度条件でのアナモックスプロセスの検討
  第52回日本水環境学会年会（2017年度）
  2018年03月17日, 口頭発表（一般）
• 遊離亜硝酸がバクテリアの活性および損傷に及ぼす影響評価
  第52回日本水環境学会年会（2017年度）
  2018年03月17日, 口頭発表（一般）
• N2O を固定するシアノバクテリアの探索とポテンシャル評価
  第52回日本水環境学会年会（2017年度）
  2018年03月17日, 口頭発表（一般）
• 繊維状担体を導入した膜分離活性汚泥法による膜詰まり抑制と再現性の確認
  第52回日本水環境学会年会（2017年度）
  2018年03月16日, 口頭発表（一般）
• 高圧ジェット装置を導入した硝化液循環活性汚泥法における余剰汚泥減容化
  化学工学会 第83年会
  2018年03月14日, 口頭発表（一般）
• 亜酸化窒素還元細菌の排水処理システムへの適用による温室効果削減:包括固定化ゲルの効果
  化学工学会 第83年会
  2018年03月14日, 口頭発表（一般）
• 散水ろ床型下水処理施設におけるバイオフィルム内の硝化細菌分布
  化学工学会 第83年会
  2018年03月13日, 口頭発表（一般）
• 低含水率馴養した種汚泥を用いた豚尿と稲わらの高温乾式メタン発酵
  第20回化学工学会学生発表会(東京大会)
  2018年03月03日, 口頭発表（一般）
• [Invited] Counter-current substrate diffusion biofilm as an innovative strategy for effective nitrogen removal from wastewaters: Principles, performances and in situ biofilm analyses
  The 8th China-Japan Symposium on Chemical Engineering
  2017年10月15日, 口頭発表（招待・特別）
• [Keynote] Nitrous oxide emission from cost-effective nitrogen removal systems: Mechanisms and mitigation strategies
  2017 International Conference on Environmental Pollution Control
  2017年10月10日, 口頭発表（基調）
• Anammoxリアクターに潜在するN2Oを巡るメタボリズム
  第20 回日本水環境学会シンポジウム
  2017年09月26日, ポスター発表
• [招待講演] バイオフィルムに関する最新の研究動向：形成抑制から利用技術まで
  第20 回日本水環境学会シンポジウム
  2017年09月26日, 口頭発表（招待・特別）
• サスペンションゲル化法を用いた微生物固定化多孔質ゲル粒子の開発と応用
  化学工学会 第49回秋季大会
  2017年09月21日, 口頭発表（一般）
• [招待講演] 亜酸化窒素還元細菌の探索・有効利用による排水処理施設からの温室効果ガス削減
  化学工学会 第49回秋季大会
  2017年09月20日, 口頭発表（招待・特別）
• 排水処理施設で発生する亜酸化窒素を還元する細菌の活性は酸素濃度によりどのように影響するのか？
  環境微生物系学会合同大会2017
  2017年08月29日, ポスター発表
• Startup of a pilot-scale anammox reactor by granular sludge inoculation for treatment of waste brine from a natural gas plant
  WET2017
  2017年07月22日, ポスター発表
• [Invited] Membrane-aerated biofilm reactors for nitrogen removal and mitigation of nitrous oxide emission from wastewater streams
  EnvironmentAsia 2017
  2017年06月22日, 口頭発表（招待・特別）
• Experiences in the Implementation on Recovery of Anammox-Enriched Cultures after Starvation Conditions on Biofilm System
  IWA 10th International Conference on Biofilm Reactors
  2017年05月11日, 口頭発表（一般）
• Identifying Hotspots for Production and Consumption of NO and N2O in a Membrane-Aerated Biofilm for Nitrogen Removal
  IWA 10th International Conference on Biofilm Reactors
  2017年05月10日, 口頭発表（一般）
• [Keynote] Application of counter-diffusion biofilm to biological nitrogen removal from wastewater: Performance, microbial community and mitigation of nitrous oxide emission
  6th Annual General Meeting of the Ireland Chinese Association of Environment, Resources & Energy (Ireland-CAERE)
  2017年05月08日, 口頭発表（基調）
• [招待講演] バイオフィルムと材料表面：細菌の固体表面への接着・遊離とバ イオフィルム形成は制御できるのか
  第 90 回日本細菌学会総会
  2017年03月21日, 口頭発表（招待・特別）
• 活性汚泥法への高圧噴射装置の導入による細菌・真核生物群集構造変化の追跡
  第51回日本水環境学会年会（2016年度）
  2017年03月16日, 口頭発表（一般）
• 天然ガス湧出地域におけるメタン資化細菌の探索とポテンシャル評価
  第51回日本水環境学会年会（2016年度）
  2017年03月15日, 口頭発表（一般）
• 稲わらと豚尿の高温乾式メタン発酵における含水率の影響
  第19回化学工学会学生発表会【東日本】
  2017年03月14日, 口頭発表（一般）
• 牛尿を用いた水稲栽培における 温室効果ガス放出、重金属蓄積の評価及び削減
  第19回化学工学会学生発表会【東日本】
  2017年03月14日, 口頭発表（一般）
• 亜酸化窒素を還元する脱窒細菌の動力学的解析:酸素暴露の影響
  化学工学会 第82年会 (東京 2017)
  2017年03月07日, 口頭発表（一般）
• 高濃度にリンを含む食品製造排水からの生物学的リン除去
  化学工学会 第82年会 (東京 2017)
  2017年03月07日, 口頭発表（一般）
• 高圧噴射装置の導入による活性汚泥中の細菌・真核生物群集構造変化の追跡
  化学工学会 第82年会 (東京 2017)
  2017年03月06日, 口頭発表（一般）
• Improving dewaterability of anaerobically digested sludge by combination of persulfate and zero valent iron
  化学工学会 第82年会 (東京 2017)
  2017年03月06日, 口頭発表（一般）
• ハスの温室効果ガス放出の評価および機構解明
  第19回化学工学会学生発表会【東日本】
  2017年03月04日, 口頭発表（一般）
• 亜酸化窒素還元に寄与する脱窒細菌の亜酸化窒素と酸素を巡るダイナミクスの動力学的評価
  日本微生物生態学会　第31回大会
  2016年10月23日, 口頭発表（一般）
• Spatial distribution of microbial community and N2O depth profiles in counter- and co- diffusion biofilms functioning simultaneous nitrification and denitrification
  IWA MEWE Biofilm Conference 2016
  2016年09月06日, 口頭発表（一般）
• 流動する固定床の開発と実槽実験報告
  第51回日本水環境学会年会（2016年度）
  2016年03月17日, 口頭発表（一般）
• ハス（Nelumbo nucifera）の温室効果ガス放出
  第51回日本水環境学会年会（2016年度）
  2016年03月17日, 口頭発表（一般）
• 酸素の存在が亜酸化窒素の還元活性に及ぼす影響：単離菌株による呼吸活性の動力学的評価
  第51回日本水環境学会年会（2016年度）
  2016年03月17日, 口頭発表（一般）
• 15N ガス希釈法による脱窒速度の測定
  第51回日本水環境学会年会（2016年度）
  2016年03月17日, 口頭発表（一般）
• 15N トレーサー法と同位体比アナライザーの併用による部分硝化リアクターの亜酸化窒素生成機構解明
  第51回日本水環境学会年会（2016年度）
  2016年03月17日, 口頭発表（一般）
• 棲み分け現象を利用した基質対向拡散型バイオフィルムによる亜酸化窒素還元細菌の集積化
  第51回日本水環境学会年会（2016年度）
  2016年03月17日, 口頭発表（一般）
• 乾式メタン発酵残渣を施用したハス田のレンコン収量及び水質への影響
  第51回日本水環境学会年会（2016年度）
  2016年03月17日, 口頭発表（一般）
• 繊維状担体を導入したハイブリッド型膜分離活性汚泥法による膜ファウリング抑制
  第51回日本水環境学会年会（2016年度）
  2016年03月15日, 口頭発表（一般）
• 稲わらと豚尿を用いた高温乾式メタン発酵における基質と菌体の関連性
  化学工学会 第81年会 (2016)
  2016年03月13日, 口頭発表（一般）
• 稲わらを基質とする豚尿浸漬型回分乾式メタン発酵における基質/種汚泥比の影響
  化学工学会 第47回秋季大会
  2015年09月11日, 口頭発表（一般）
• 海水淡水化逆浸透膜に形成されるバイオフィルムの解析と簡易モニタリング方法の開発
  化学工学会 第47回秋季大会
  2015年09月11日, 口頭発表（一般）
• 高圧噴射装置の設置による余剰汚泥減容化とそれに伴う微生物群集の変化
  化学工学会 第47回秋季大会
  2015年09月09日, 口頭発表（一般）
• 高圧ジェット装置による汚泥減容化が活性汚泥に棲息する細菌・真核生物群集構造に与える影響
  化学工学会　第80年会
  2015年03月21日, 口頭発表（一般）
• 豚尿と稲わらの高温乾式メタン発酵における豚尿循環および基質混合方式の検討
  化学工学会　第80年会
  2015年03月21日, 口頭発表（一般）
• ガス透過膜を用いた高活性な亜酸化窒素還元細菌の集積化と分離培養
  化学工学会　第80年会
  2015年03月20日, 口頭発表（一般）
• 水田に棲息する脱窒性メタン酸化細菌の土壌空間分布とニッチ
  化学工学会　第80年会
  2015年03月19日, 口頭発表（一般）
• フッ素系ポリマーコーティング材料によるバイオフィルムの形成抑制
  化学工学会　第80年会
  2015年03月19日, 口頭発表（一般）
• 高圧ジェット装置を用いた活性汚泥減容化プロセスの減容化性能と微生物群集構造
  第50回日本水環境学会年会（2015年度）
  2015年03月17日, 口頭発表（一般）
• 亜酸化窒素還元細菌の単離に向けた基質対向拡散型集積培養装置の開発
  第50回日本水環境学会年会（2015年度）
  2015年03月17日, 口頭発表（一般）
• 連続式および半回分式部分硝化リアクターからの亜酸化窒素生成メカニズム
  第50回日本水環境学会年会（2015年度）
  2015年03月17日, 口頭発表（一般）
• 高濃度窒素含有廃水の処理に向けた部分硝化型リアクターの亜硝酸化性能と硝化細菌ダイナミクス
  第50回日本水環境学会年会（2015年度）
  2015年03月17日, 口頭発表（一般）
• [招待講演］バイオフィルムの形成抑制を目指した水処理用バイオインターフェイスの機能化
  環境微生物系学会合同大会2014
  2014年10月24日, 口頭発表（招待・特別）
• バイオファウリングを防止する複合酵素固定化型ろ過膜の開発：固定化酵素の効果
  環境微生物系学会合同大会2014
  2014年10月23日, ポスター発表
• 比較解析から迫る亜硝酸酸化細菌Nitrospiraの生態学的ニッチ
  環境微生物系学会合同大会2014
  2014年10月23日, ポスター発表
• 基質対向拡散型培養装置によるN2O還元細菌集積系の細菌叢変遷とN2O還元活性
  環境微生物系学会合同大会2014
  2014年10月23日, ポスター発表
• 15Nトレーサー法による回分式・連続式部分硝化リアクターからの亜硝酸窒素生成機構の解明
  環境微生物系学会合同大会2014
  2014年10月23日, ポスター発表
• Nitrous oxide production pathways of nitrifying activated sludge in a sequencing batch reactor
  IWA World Water Congress
  2014年09月24日, 口頭発表（一般）
• ［招待講演］材料の表面改変によるバイオフィルム構造・機能の制御
  化学工学会第46回秋季大会シンポジウム
  2014年09月17日, 口頭発表（招待・特別）
• ［招待講演］クオラムセンシング機構抑制のための 材料界面の機能化：バイオフィルム制御に向けて
  第66回日本生物工学会大会
  2014年09月11日, 口頭発表（招待・特別）
• ［受賞講演］半回分式膜曝気型バイオフィルムリアクターの低C/N比排水からの窒素・リン同時除去の適用性
  第17回日本水環境学会シンポジウム
  2014年09月09日, 口頭発表（招待・特別）
• Identification of nitrous oxide-reducing bacteria in activated sludge by 13C-acetate probing combined with high-throughput sequencing
  ISME 15
  2014年08月25日, ポスター発表
• [Keynote] Nitrous oxide production in a nitrogen removal process: Its mechanisms and mitigation strategies
  3rd International Conference on Environmental Engineering, Science and Management
  2014年03月26日, 口頭発表（基調）
• 高圧ジェット装置を用いた活性汚泥減容化プロセスの減容化性能と微生物群集構造
  水環境学会第48回年会
  2014年03月19日, 口頭発表（一般）
• 落水後の水田土壌から排出される亜酸化窒素放出機構の解明
  化学工学会第79年会
  2014年03月18日, ポスター発表
• 乾式メタン発酵残渣を施用した多収米飼料イネ水田における窒素収支の評価
  化学工学会第79年会
  2014年03月18日, ポスター発表
• 豚ふん尿と河川敷のバイオマスとの高温乾式メタン発酵特性の解明
  化学工学会第79年会
  2014年03月18日, ポスター発表
• 亜酸化窒素還元細菌の単離に向けた基質対向拡散型集積培養装置の開発
  水環境学会第48回年会
  2014年03月17日, 口頭発表（一般）
• 連続式および半回分式部分硝化リアクターからの亜酸化窒素生成メカニズム
  水環境学会第48回年会
  2014年03月17日, 口頭発表（一般）
• 高濃度窒素含有廃水の処理に向けた部分硝化型リアクターの亜硝酸化性能と硝化細菌ダイナミクス
  水環境学会第48回年会
  2014年03月17日, 口頭発表（一般）
• 乾式メタン発酵残渣を施肥した多収米飼料イネ栽培における温室効果ガス排出量の把握と削減
  第16回化学工学会学生発表会
  2014年03月01日, 口頭発表（一般）
• 乾式メタン発酵残渣を施用した多収米飼料イネ水田における窒素挙動の把握
  第16回化学工学会学生発表会
  2014年03月01日, 口頭発表（一般）
• 水田中に棲息する脱窒性メタン細菌の空間分布と系統学的評価
  第29回日本微生物生態学会大会
  2013年11月25日, ポスター発表
• 部分硝化型活性汚泥バイオリアクターにおける亜酸化窒素放出と放出特性の解明：ヒドロキシルアミンの影響
  第29回日本微生物生態学会大会
  2013年11月23日, ポスター発表
• Diversity of total and functional microbiome of anammox reactors fed with complex and synthetic nitrogen-rich wastewaters
  3rd International Conference on Nitrification
  2013年09月05日, 口頭発表（一般）
• Spatio-temporal analysis of nitrous oxide production and emission after drainage of flooded soil
  3rd International Conference on Nitrification
  2013年09月05日, 口頭発表（一般）
• Implications of predominant ammonia oxidizing bacteria and reactor conditions on nitrous oxide production from a partial nitrifying bioreactor
  3rd International Conference on Nitrification
  2013年09月05日, 口頭発表（一般）
• Reduction of excess activated sludge from wastewater treatment and feasibility of bacterial population control by a high pressure jet device
  Microbial Ecology and Water Engineering 2013
  2013年07月09日, ポスター発表
• [Keynote] Control of Micro-Environments in Biofilms by a Gas-Permeable Membrane for Single-Stage Nutrient and Xenobiotic Removal
  Microbial Ecology and Water Engineering 2013
  2013年07月09日, 口頭発表（招待・特別）
• Nitrification in Extremely Low Dissolved Oxygen Conditions
  Microbial Ecology and Water Engineering 2013
  2013年07月08日, 口頭発表（一般）
• Applicability of a sequencing batch membrane biofilm reactor for simultaneous nitrogen and phosphorus removal from low C/N ratio wastewater
  Water and Environment Technology Conference 2013
  2013年06月16日, 口頭発表（一般）
• Reduction of excess activated sludge by a high-pressure-flow device (DEM) and the transition of microbial community: Pilot-scale demonstration
  Water and Environment Technology Conference 2013
  2013年06月15日, 口頭発表（一般）
• Phylogeny and abundance of bacteria responsible for nitrous oxide reduction from activated sludge systems
  Water and Environment Technology Conference 2013
  2013年06月15日, 口頭発表（一般）
• 基盤表面改質によるバイオフィルム制御と環境バイオテクノロジーへの応用
  化学工学会第78年会
  2013年03月19日, 口頭発表（招待・特別）
• 地域条件を反映した除染計画の策定
  化学工学会 第78年会
  2013年03月18日, 口頭発表（一般）
• 大型糸状緑藻を生育させた水路による農地排水処理
  化学工学会第78年会
  2013年03月17日, 口頭発表（一般）
• 豚ふん尿と稲ワラを用いた高温乾式メタン発酵プロセスの開発および循環型豚ふん尿処理システムの実現可能性評価
  化学工学会第78年会
  2013年03月17日, 口頭発表（一般）
• 畜産排水を施肥した飼料イネ水田におけるCH4およびN2Oの同時削減の試み
  化学工学会第78年会
  2013年03月17日, 口頭発表（一般）
• 不織布担体の化学改変によるアンモニア酸化細菌のバイオフィルム形成促進
  化学工学会 第78年会
  2013年03月17日, 口頭発表（一般）
• 高圧噴射装置による下水汚泥減容化プロセスの可能性と細菌群集構造解析:パイロットスケール試験
  化学工学会 第78年会
  2013年03月17日, 口頭発表（一般）
• 豚ふん尿と様々な炭素源との高温乾式メタン発酵特性の解明
  化学工学会 第78年会
  2013年03月17日, 口頭発表（一般）
• 異なる水環境におけるAnammox反応活性の評価
  第47回日本水環境学会年会
  2013年03月13日, 口頭発表（一般）
• 空間分布解析による落水後の水田における突発的な亜酸化窒素排出挙動の解明
  第47回日本水環境学会年会
  2013年03月13日, 口頭発表（一般）
• 高圧噴射装置を用いた下水汚泥減容化プロセスのパイロットスケールによるコンセプト実証と菌叢群集構造解析
  第47回日本水環境学会年会
  2013年03月12日, 口頭発表（一般）
• 部分硝化型バイオリアクターで優占化された異なるアンモニア酸化細菌群の亜酸化窒素生成ポテンシャルと経路の解析
  第47回日本水環境学会年会
  2013年03月12日, 口頭発表（一般）
• 不織布担体の化学改変によるAOBバイオフィルム形成促進
  第64回日本生物工学会大会
  2012年10月26日, 口頭発表（一般）
• 微生物関情報伝達物質を分解するろ過膜によるバイオフィルムの抑制
  第64回日本生物工学会大会
  2012年10月26日, 口頭発表（一般）
• 畜産排水を施肥した飼料イネ水田のメタン放出挙動とメタンをめぐる微生物群のダイナミクス
  第64回日本生物工学会大会
  2012年10月26日, 口頭発表（一般）
• 豚ふん尿と稲ワラを用いた高温乾式メタン発酵プロセスの開発
  第64回日本生物工学会大会
  2012年10月26日, 口頭発表（一般）
• 部分硝化プロセスからの亜酸化窒素の発生量・発生起源―溶存酸素濃度の影響
  第64回日本生物工学会大会
  2012年10月26日, 口頭発表（一般）
• 排水再生施設におけるRO膜のバイオファウリング物質の組成解析
  2012年度バイオフィルムと複合系研究会
  2012年10月12日, ポスター発表
• 不織布担体の化学改変によるAOBのバイオフィルム形成促進
  2012年度バイオフィルムと複合系研究会
  2012年10月12日, 口頭発表（一般）
• Confronting hydroxylamine with nitrite triggers N2O production from nitrifying bioreactors towards nitritation
  IWA Nutrient Removal & Recovery 2012: Trends in NRR
  2012年09月24日, 口頭発表（一般）
• Methane flux and abundance of microorganisms responsible for methane production and oxidation in rice paddies applying liquid manure from livestock wastewater with different irrigation strategies
  The 28th Annual Meeting of the Japanese Society of Microbial Ecology
  2012年09月21日, ポスター発表
• Biofilm control in wastewater treatment by modification of bio-interfaces: Towards enhancement of biofilm reactor performance and prevention of biofouling
  The 28th Annual Meeting of the Japanese Society of Microbial Ecology
  2012年09月20日, 口頭発表（招待・特別）
• Responses of soil nitrite-oxidizers to interacting global change components
  The 14th International Symposium on Microbial Ecology
  2012年08月23日, 口頭発表（一般）
• Efficient nitrifying bioreactors operated at extremely low dissolved oxygen concentrations produce low N2O emissions
  The 14th International Symposium on Microbial Ecology
  2012年08月23日, 口頭発表（一般）
• Elucidating nitrous oxide formation pathways in a nitrifying bioreactor towards nitrification: the effect of dissolved oxygen
  The 14th International Symposium on Microbial Ecology
  2012年08月22日, 口頭発表（一般）
• Production mechanism of nitrous oxide from a nitrifying bioreactor towards partial ammonium oxidation
  Water and Environment Technology Conference
  2012年06月30日, 口頭発表（一般）
• 養豚排水処理に向けた乾式メタン発酵の性能評価および循環型システムの構築
  化学工学会第77年会
  2012年03月17日, 口頭発表（一般）
• バイオフィルム由来の目詰まりを抑制する酵素固定化型ろ過膜の開発
  化学工学会第77年会
  2012年03月17日, 口頭発表（一般）
• 畜産排水を施肥した水田における亜酸化窒素の動態とアンモニア酸化に関与する遺伝子発現解析
  第46回日本水環境学会年会
  2012年03月16日, 口頭発表（一般）
• 畜産排水を施肥した水田におけるメタン放出の挙動とメタン酸化細菌の関係解析
  第46回日本水環境学会年会
  2012年03月16日, 口頭発表（一般）
• 微生物情報伝達を遮断する機能性ろ過膜における膜の目詰まり防止の試み
  第46回日本水環境学会年会
  2012年03月16日, 口頭発表（一般）
• 酸素分圧の違いがアンモニア酸化細菌の群集構造と亜硝酸蓄積・亜酸化窒素放出へ及ぼす影響
  第46回日本水環境学会年会
  2012年03月14日, 口頭発表（一般）
• 亜硝酸型バイオリアクターにおける亜酸化窒素放出量および放出起源の解析
  第46回日本水環境学会年会
  2012年03月14日, 口頭発表（一般）
• Simultaneous removals of azo dye and nitrogenous compounds by a membrane-aerated biofilm
  IWA Biofilm Conference 2011 Processes in Biofilms
  2011年10月29日, 口頭発表（一般）
• Who is who? Assessing the microbial diversity in wastewater treatment biofilms for completely autotrophic nitrogen removal
  IWA Biofilm Conference 2011 Processes in Biofilms
  2011年10月29日, 口頭発表（一般）
• 両性イオン基を導入した高分子材料によるバイオフィルム抑制
  バイオフィルムと複合系研究会
  2011年10月22日, 口頭発表（一般）
• 加工不織布担体を用いたアンモニア酸化細菌のバイオフィルム形成促進の試み
  バイオフィルムと複合系研究会
  2011年10月22日, 口頭発表（一般）
• 微生物間情報伝達を遮断する機能性ろ過膜によるバイオファウリング防止の試み
  バイオフィルムと複合系研究会
  2011年10月22日, 口頭発表（一般）
• Nitrogen Dynamics in Forage Rice Field Treating Liquid Cattle Waste
  4th IWA-ASPIRE Conference&Exhibition
  2011年10月03日, 口頭発表（一般）
• Effect of Operational Regime on Successful Nitritation and Nitrifying Bacterial Community in an Autotrophic Nitrifying Bioreactor
  4th IWA-ASPIRE Conference&Exhibition
  2011年10月03日, 口頭発表（一般）
• 亜硝酸化型窒素除去を志向した活性汚泥槽からの亜酸化窒素発生機構の解析
  第63回日本生物工学会大会
  2011年09月26日, 口頭発表（一般）
• 河川底質に棲息するAnammox細菌の同定および定量
  第63回日本生物工学会大会
  2011年09月26日, 口頭発表（一般）
• ガス透過膜を用いた単一槽型亜硝酸化-Anammoxリアクターの亜酸化窒素放出特性
  第14回水環境学会シンポジウム
  2011年09月10日, 口頭発表（一般）
• 飼料イネ水田を利用した温室効果ガス低排出型畜産排水処理の開発
  第14回水環境学会シンポジウム
  2011年09月10日, 口頭発表（一般）
• Production and mitigation of N2O in sequentially membrane-aerated redox-stratifed nitritation/anammox biofilms
  2nd International Conference on Nitrification
  2011年07月07日, 口頭発表（一般）
• The significance of predominant ammonia-oxidizing bacteria on nitritation performance and nitrous oxide emission
  2nd International Conference on Nitrification
  2011年07月07日, 口頭発表（一般）
• 排水の流入形式が亜硝酸化とアンモニア酸化細菌の優占種に及ぼす影響
  化学工学会第76年会
  2011年03月22日, 口頭発表（一般）
• ガス透過膜上に形成するバイオフィルムを利用した染色排水からのアゾ色素および窒素化合物の同時除去
  化学工学会第76年会
  2011年03月22日, 口頭発表（一般）
• メンブレンエアレーション型バイオフィルムリアクターによるアゾ色素および窒素化合物の単一槽内同時除去
  第45回日本水環境学会年会
  2011年03月19日, 口頭発表（一般）
• 15Nを用いた畜産排水施用水田の窒素挙動の解明
  第45回日本水環境学会年会
  2011年03月19日, 口頭発表（一般）
• 畜産排水除去を志向した飼料イネ水田におけるPlanctomycetes門の生態構造解析
  第45回日本水環境学会年会
  2011年03月18日, 口頭発表（一般）
• 飼料イネ水田による畜産排水処理における温室効果ガス排出
  第45回日本水環境学会年会
  2011年03月18日, 口頭発表（一般）
• 増殖速度が異なる硝化細菌群が有する硝化能および亜硝酸蓄積能
  第45回日本水環境学会年会
  2011年03月18日, 口頭発表（一般）
• バイオファウリングを抑制する水・排水処理用ろ過膜の作製
  バイオフィルム研究会設立10周年記念夏の学校
  2010年09月10日, ポスター発表
• 材料表面の物理化学的性状の改変によるバイオフィルム抑制―放射線グラフト重合法による抗菌材料の開発―
  バイオフィルム研究会設立10周年記念夏の学校
  2010年09月10日, ポスター発表
• Ecophysiology and diversity of Chloroflexi in an anammox biofilm grown in an anaerobic fixed-bed upflow reactor
  13th International Symposium on Microbial Ecology
  2010年08月27日, 口頭発表（一般）
• Aggregate size and architecture determine biomass activity for one-stage partial nitritation and anammox
  13th International Symposium on Microbial Ecology
  2010年08月26日, 口頭発表（一般）
• Inoculum effects on community composition and nitritation success of nitrifying biofilm grown on a gas-permeable membrane
  WEF/IWA Biofilm Reactor Technology Conference 2010
  2010年08月16日, 口頭発表（一般）
• Population of Ammonia- and Nitrite- Oxidizing Bacteria Compromises Successful Nitritation in a Membrane-Aerated Biofilm Reactor.
  5th IWA International Young Water Professionals Conference
  2010年07月06日, 口頭発表（一般）
• Enhancing nitrogen and phosphorus removal in an Anaerobic/Oxic/Anoxic sequencing batch reactor: The dependence of the amount of external carbon
  Water and Environment Technology Conference 2010
  2010年06月26日, 口頭発表（一般）
• Microbial community stratification in membrane-aerated biofilm reactors for comletely autotrophic nitrogen removal
  Water Research Conference
  2010年04月, 口頭発表（一般）
• 異なる表面電位を有する材料表面への微生物の初期付着とバイオフィルム構造の関係解明
  化学工学会 第75年会
  2010年03月18日, 口頭発表（一般）
• Denitrification potential of an anammox biofilm grown in a fixed-bed upflow reactor: Ecophysiology and diversity of Chloroflexi
  日本水環境学会第26回大会
  2010年03月17日, 口頭発表（一般）
• 異なる表面電位を有する材料表面への 微生物の初期付着とバイオフィルム構造の関係解明
  2009年バイオフィルム研究会
  2009年11月21日, 口頭発表（一般）
• 異なる表面電位を有する材料表面への 微生物の初期付着とバイオフィルム構造の関係解
  2009年度バイオフィルムと複合系研究会
  2009年10月12日, 口頭発表（一般）
• 窒素化合物の効率除去を志向した 複合微生物系バイオフィルム制御の試み
  2009年度バイオフィルムと複合系研究会
  2009年10月12日, 口頭発表（一般）
• One-stage partial nitritation and anammox to remove nitrogen from digested black water
  IWA 2nd Specialized Conference Nutrient Management in Wastewater Treatment Processes
  2009年09月07日, 口頭発表（一般）
• Start-up strategies of membrane-aerated biofilm reactor (MABR) for completely autotrophic nitrogen removal
  IWA 2nd Specialized Conference Nutrient Management in Wastewater Treatment Processes
  2009年09月07日, 口頭発表（一般）
• Abundance and diversity of microbial communities in long-term operated anammox biofilm reactors initiated with different inocula
  IWA Specialised Conference on Microbial Population Dynamics in Biological Wastewater Treatment
  2009年05月26日, 口頭発表（一般）
• Initial population of ammonia- and nitrite- oxidizing bacteria compromises successful nitritation in a counter-diffusion biofilm geometry
  IWA Specialised Conference on Microbial Population Dynamics in Biological Wastewater Treatment
  2009年05月26日, ポスター発表
• Microbial community analysis in an autotrophic hollow-fiber membrane-aerated biofilm reactor (HFMBR) treating a high-strength nitrogen wastewater
  IWA Specialised Conference on Microbial Population Dynamics in Biological Wastewater Treatment
  2009年05月26日, 口頭発表（一般）
• Microbial population dynamics during start up of nitritation in redox-stratified biofilms: modelling and experimental comparison
  IWA Specialised Conference on Microbial Population Dynamics in Biological Wastewater Treatment
  2009年05月26日, ポスター発表
• 半回分式メンブレンバイオフィルムリアクタによる窒素・リン同時除去：低C/N比排水への適用
  第43回日本水環境学会年会
  2009年03月, ポスター発表
• Nitrifier and anammox population dynamics in an autotrophic nitrogen removal membrane biofilm reactor
  WEFTECH 2008
  2008年10月, 口頭発表（一般）
• Dynamics of spatial distribution and microbial activity of nitrifying populations in redox-stratified biofilms: modeling and experimental investigations
  BIOFILMS III
  2008年10月, 口頭発表（一般）
• Do different initial microbial communities converge in identically operated anammox biofilm reactors?
  BIOFILMS III
  2008年10月, 口頭発表（一般）
• Ammonium removal performance and spatial distribution of nitrifying bacterial populations in autotrophic co- and counter-diffusion biofilms under oxygen limited conditions.
  IWA World Water Congress and Exhibition
  2008年09月, 口頭発表（一般）
• Redox stratification controlled biofilm reactors for completely autotrophic nitrogen removal
  IWA North American Membrane Research Conference
  2008年08月, 口頭発表（招待・特別）
• Microbial population dynamics in redox-stratified biofilms during start-up of autotrophic nitrogen removal reactors
  The 12th International Symposium on Microbial Ecology (ISME-12)
  2008年08月, 口頭発表（一般）
• Redox stratification controlled biofilm reactors for completely autotrophic nitrogen removal
  5th IWA Leading-Edge Conference & Exhibition on Water and Wastewater Technologies
  2008年06月, ポスター発表
• Gaseous nitrogen oxide emissions, nitrogen removal performance, and population dynamics in hollow fiber membrane-aerated biofilm reactors
  World Environmental & Water Resources Congress
  2008年05月, 口頭発表（一般）
• SBMBfR による生物学的窒素・リン除去技術の開発と低C/N比排水への適用
  化学工学会第73年会
  2008年03月, 口頭発表（一般）
• Controlling gaseous nitrogen oxide emissions and nitrogen removal performance in hollow fiber membrane aerated biofilm reactors
  The WEF Membrane Technology 2008 Conference
  2008年01月, 口頭発表（一般）
• Effect of membrane surface functionalization on formation and shear resistance of nitrifying biofilms
  IWA Biofilm Technology
  2008年01月, 口頭発表（一般）
• Start-up strategies for stable autotrophic nitrogen removal in redox stratification controlled biofilm reactor (ReSCoBiR)
  IWA Biofilm Technology
  2008年01月, 口頭発表（一般）
• Assessment of the biofilm model implementing kinetic parameters estimated by respirometric technique through comparison with experimental data
  IWA Biofilm Technology
  2008年01月, ポスター発表
• Sequencing batch membrane biofilm reactor for simultaneous nitrogen and phosphorus removal: Effect of membrane biofilm and applicability to low COD/N wastewater
  BioMicroWorld 2007
  2007年12月, ポスター発表
• 電気泳動による微生物固定化空間内の基質輸送の促進と脱窒反応の向上
  化学工学会第39回秋季大会
  2007年09月, 口頭発表（招待・特別）
• 固体表面電位が微生物付着とバイオフィルム形成に及ぼす影響
  第9回化学工学会学生発表会
  2007年03月, 口頭発表（一般）
• Redox-stratification controlled biofilm for completely autotrophic nitrogen removal: Modeling the effect of substrate co- versus counter-diffusion on performance
  Biofilm Systems IV
  2006年09月, 口頭発表（一般）
• Experimental and simulation analysis of community structure of nitrifying bacteria in a membrane-aerated biofilm
  Biofilm Systems VI
  2006年09月, 口頭発表（一般）
• Combined approach for characterization of community structure in nitrifying granules as evaluated by experimental and 2-dimensional biofilm model analyses
  Granule Workshop II
  2006年09月, 口頭発表（一般）
• 固体表面の物理化学的性状がバイオフィルム形成に及ぼす影響−フローセル試験とシミュレーション解析−
  第22回日本微生物生態学会
  2006年06月, その他
• 硝化グラニュールの微生物生態構造解析
  第22回日本微生物生態学会
  2006年06月, ポスター発表
• 好気性グラニュールの微生物生態構造解析
  環境バイオテクノロジー学会2006年度大会
  2006年06月, ポスター発表
• メンブレンエアレーション法を導入した半回分式リアクターによる新しい排水処理技術
  環境バイオテクノロジー学会2006年度大会
  2006年06月, ポスター発表
• Positively charged surface is a determining factor on nascent bacterial adhesion and subsequent biofilm formation
  BIOFILMS II Attachment and Detachment in Pure and Mixed Cultures
  2006年03月, ポスター発表
• Experimental support for the individual-based modeling of a membrane-aerated biofilm
  BIOFILMS II Attachment and Detachment in Pure and Mixed Cultures
  2006年03月, ポスター発表
• バイオフィルムにおける微生物生態構造のシミュレーション解析および実験的解明
  化学工学会第71年会
  2006年03月, 口頭発表（一般）
• 単一槽内での独立栄養性窒素除去を志向した新しい生物膜法の性能予測 —並行拡散方式と対向拡散方式の比較—
  日本水環境学会第40回年会
  2006年03月, 口頭発表（一般）
• メンブレンエアレーションバイオフィルムにおける実験的解明およびシミュレーションモデルの構築
  日本微生物生態学会第21回大会
  2005年10月, ポスター発表
• バイオフィルム内の微生物生態構造の実験的解明およびシミュレーション解析
  化学工学会関東支部50周年記念大会
  2005年08月, 口頭発表（一般）
• 材料表面の物理化学的性質が微生物付着およびバイオフィルム形成に及ぼす影響の評価
  化学工学会第70年会
  2005年03月, 口頭発表（一般）
• トップダウンおよびメンブレンエアレーションバイオフィルムにおける硝化細菌群の生態構造の比較
  化学工学会第70年会
  2005年03月, 口頭発表（一般）
• メンブレンエアレーション型SBRによる窒素・リン同時除去
  日本水環境学会第39回年会
  2005年03月, 口頭発表（一般）
• 材料表面の物理化学的性質と微生物付着性および付着時の活性との関係
  日本微生物生態学会第20回大会バイオフィルム研究部会
  2004年11月, 口頭発表（一般）
• 水素供与型メンブレン及び繊維状担体を用いた脱窒リアクターの開発
  日本水処理生物学会第41回大会
  2004年11月, 口頭発表（一般）
• Comparison of spatial organization in top-down- and membrane-aerated biofilms: A numerical study
  International Conference Biofilms 2004: Structure and Activity of Biofilms
  2004年10月, 口頭発表（一般）
• Feasibility study of a membrane-aerated biofilm reactor to achieve controllable nitrification under oxygen-depleted conditions
  IWA 4th World Water Congress
  2004年09月, 口頭発表（一般）
• 材料表面の物理化学的性質と微生物の付着性との関係解析
  化学工学会秋田大会
  2004年07月, 口頭発表（一般）
• Enhancement of biofilm formation onto surface-modified hollow-fiber membranes and its application to membrane-aerated biofilm reactor
  5th International Conference on Biofilm Systems
  2003年09月, ポスター発表
• メンブレンエアレーション法による低C/N比からの有機物・栄養塩同時除去
  日本水環境学会第37回年会
  2003年03月, 口頭発表（一般）
• Development of a membrane-aerated biofilm reactor applicable to ammonia removal from industrial wastewater
  The 2nd Joint China/Japan Chemical Engineering Symposium
  2002年11月, 口頭発表（一般）
• Structure and activity of a biofilm in a membrane-aerated biofilm reactor applicable to simultaneous nitrification and denitrification
  Biofilms in Industry, Medicine and Environmental Biotechnology
  2002年08月, ポスター発表
• メンブレンエアレーションリアクタの窒素除去特性と生物膜解析
  化学工学会第67年会
  2002年03月, 口頭発表（一般）
• 生物膜内における基質・酸素浸透深さおよび微生物活性部位の流入負荷変動に対する応答性
  化学工学会第66年会
  2001年04月, 口頭発表（一般）
• 微小電極法を用いた生物膜内基質濃度分布の測定
  化学工学会第33回秋季大会
  2000年09月, 口頭発表（一般）

外部研究資金等

• 亜酸化窒素削減に寄与する細菌の探索と排水処理施設への適用に向けた機構モデルの開発
  共同研究, 自 2016年04月01日, 至 2016年08月31日
• MBCテーブルテストに関する研究
  共同研究, 自 2016年03月07日, 至 2018年03月31日

委員歴

• 独立行政法人日本学術振興会
  国際事業委員会書面審査員・書面評価員
  自 20220701, 至 20230630
• 一般社団法人リン循環産業振興機構
  監事
  自 20200601, 至 2022525
• 国際水学会バイオフィルム専門家グループ
  運営委員
  自 201706, 至 20220331
• 国際水学会微生物生態と水工学専門家グループ
  運営委員
  自 201309, 至 20220331

受賞

• 公益社団法人 日本水環境学会
  日本水環境学会 論文賞
  Organic carbon determines nitrous oxide consumption activity of clade I and II nosZ bacteria: Genomic and biokinetic insights
  論文賞（The JSWE Paper Award） は公益社団法人 日本水環境学会の本会会員にして、水環境分野における学術・技術的に優れた一連の研究に基づく論文を発表した個人に授与される。
  2023年06月13日
• 日本水環境学会
  2020年 年間優秀論文賞
  Excess Sludge Reduction Using a High-pressure Jet Device via a Modified Ludzack-Ettinger Process: Performance and Microbial Communities
  2021年09月
• 日本下水道協会
  2019年優秀論文賞
  最初沈殿池汚泥から回収した繊維状物の脱水助材としての利用が嫌気性消化を伴う下水汚泥処理に及ぼす影響
  2020年06月