大松　勉OMATSU Tsutomuオオマツ　ツトム

業績情報

氏名・連絡先

• 氏名

  オオマツ　ツトム, 大松　勉, OMATSU Tsutomu

• 個人ホームページ

  https://sites.google.com/view/teamomatsu

主たる所属・職名

• 農学部附属感染症未来疫学研究センター, 准教授

その他の所属

• 農学部附属感染症未来疫学研究センター
• 農学研究院
• 農学府
• 農学部
• グローバルイノベーション研究院

経歴

• -
  岐阜大学大学院　連合獣医学研究科　講師
  自 2015年04月01日
• -
  東京農工大学　農学部附属国際家畜感染症防疫研究教育センター　家畜感染症経済分析学研究部門 講師
  自 2012年09月01日
• -
  大塚製薬株式会社　新薬開発本部　開発部
  自 2010年05月01日, 至 2012年08月31日
• -
  国立感染症研究所　ウイルス第一部　第二室　協力研究員
  自 2010年04月01日
• -
  国立感染症研究所　ウイルス第一部　第二室　研究員
  自 2007年04月01日, 至 2010年03月31日

学歴

• 東京大学
  農学生命科学研究科
  獣医学専攻
  至 2007年03月, 修了, 博士
• 東京大学
  農学部
  獣医学課程　獣医学専修
  至 2003年03月, 卒業

学位

• 博士（獣医学）

教育・研究活動状況

• 家畜および野生動物を対象とした感染症の防疫に関する研究

研究分野

• A189　ライフサイエンス, A42020　獣医学
• A189　ライフサイエンス, A49060　ウイルス学

科学研究費助成事業

• 挑戦的研究（萌芽）
  猫マルチオーガンオルガノイドオンチップシステムの確立
  自 2023年, 至 2023年
• 基盤研究(B)
  尿サンプル由来オルガノイドを用いた多角的な研究:イヌ膀胱がん克服にむけて
  自 2023年, 至 2023年
• 挑戦的研究（萌芽）
  ゲーミフィケーションの活用と人文社会学を融合させた狂犬病予防のメディア教育開発
  自 2023年, 至 2023年
• 挑戦的研究（萌芽）
  耐酸性微細藻類を用いた炎症性腸疾患の新規治療法の確立
  自 2023年, 至 2024年
• 基盤研究(B)
  尿サンプル由来オルガノイドを用いた多角的な研究:イヌ膀胱がん克服にむけて
  自 2022年, 至 2022年
• 挑戦的研究（萌芽）
  猫マルチオーガンオルガノイドオンチップシステムの確立
  自 2022年, 至 2022年
• 挑戦的研究（萌芽）
  ゲーミフィケーションの活用と人文社会学を融合させた狂犬病予防のメディア教育開発
  自 2022年, 至 2022年
• 基盤研究(B)
  尿サンプル由来オルガノイドを用いた多角的な研究:イヌ膀胱がん克服にむけて
  自 2021年, 至 2021年
• 基盤研究(B)
  がん幹細胞性の維持機構は創薬標的になるか？
  自 2021年, 至 2021年
• 基盤研究(A)
  ゲノム解析と数理解析を用いた動物の社会生活による薬剤耐性菌の環境汚染の解析
  自 2021年, 至 2021年
• 挑戦的研究（萌芽）
  ゲーミフィケーションの活用と人文社会学を融合させた狂犬病予防のメディア教育開発
  自 2021年, 至 2021年
• 基盤研究(B)
  メカニカルストレスに基づいた犬脊髄損傷に対する再生医療とリハビリテーションの融合
  自 2021年, 至 2021年
• 基盤研究(B)
  メカニカルストレスに基づいた犬脊髄損傷に対する再生医療とリハビリテーションの融合
  自 2020年, 至 2020年
• 挑戦的研究（萌芽）
  ゲーミフィケーションの活用と人文社会学を融合させた狂犬病予防のメディア教育開発
  自 2020年, 至 2020年
• 基盤研究(A)
  ゲノム解析と数理解析を用いた動物の社会生活による薬剤耐性菌の環境汚染の解析
  自 2020年, 至 2020年
• 基盤研究(B)
  がん幹細胞性の維持機構は創薬標的になるか？
  自 2020年, 至 2020年
• 基盤研究(B)
  尿サンプル由来オルガノイドを用いた多角的な研究:イヌ膀胱がん克服にむけて
  自 2020年, 至 2020年
• 国際共同研究加速基金（国際共同研究強化(B)）
  疫学と数理モデルを融合したコウモリ由来感染症のリスク分析
  自 2019年, 至 2024年
• 挑戦的研究（萌芽）
  ゲーミフィケーションの活用と人文社会学を融合させた狂犬病予防のメディア教育開発
  自 2019年, 至 2019年
• 基盤研究(B)
  メカニカルストレスに基づいた犬脊髄損傷に対する再生医療とリハビリテーションの融合
  自 2019年, 至 2019年
• 基盤研究(B)海外学術調査
  翼手目由来感染症リスクの対応戦略構築のための疫学的基礎データの収集
  自 2019年, 至 2019年
• 基盤研究(B)
  ホスファターゼを標的とした新規抗がん戦略への基盤的研究
  自 2019年, 至 2019年
• 基盤研究(A)
  ゲノム解析と数理解析を用いた動物の社会生活による薬剤耐性菌の環境汚染の解析
  自 2019年, 至 2019年
• 基盤研究(B)海外学術調査
  翼手目由来感染症リスクの対応戦略構築のための疫学的基礎データの収集
  自 2018年, 至 2018年
• 基盤研究(A)
  ゲノム解析と数理解析を用いた動物の社会生活による薬剤耐性菌の環境汚染の解析
  自 2018年, 至 2018年
• 基盤研究(B)
  ホスファターゼを標的とした新規抗がん戦略への基盤的研究
  自 2018年, 至 2018年
• 基盤研究(B)海外学術調査
  翼手目由来感染症リスクの対応戦略構築のための疫学的基礎データの収集
  自 2017年, 至 2017年
• 挑戦的萌芽研究
  獲得形質の遺伝メカニズム
  自 2017年, 至 2017年
• 基盤研究(B)海外学術調査
  東南アジア・オセアニアにおけるオオコウモリ由来新興感染症の出現予測
  自 2017年, 至 2017年
• 基盤研究(B)
  ホスファターゼを標的とした新規抗がん戦略への基盤的研究
  自 2017年, 至 2017年
• 挑戦的萌芽研究
  獲得形質の遺伝メカニズム
  自 2016年, 至 2016年
• 基盤研究（B）
  東南アジア・オセアニアにおけるオオコウモリ由来新興感染症の出現予測
  自 2016年, 至 2016年
• 基盤研究(C)
  チクシトゲアリ創設女王どうしの協力行動の適応的意義と生体アミンが行動に与える影響
  自 2016年, 至 2016年
• 基盤研究(C)
  チクシトゲアリ創設女王どうしの協力行動の適応的意義と生体アミンが行動に与える影響
  自 2015年, 至 2015年
• 挑戦的萌芽研究
  低重力環境で起こりうる次世代の形態・機能変化とのその分子基盤
  自 2015年, 至 2015年
• 基盤研究（B）
  東南アジア・オセアニアにおけるオオコウモリ由来新興感染症の出現予測
  自 2015年, 至 2015年
• 挑戦的萌芽研究
  低重力環境で起こりうる次世代の形態・機能変化とのその分子基盤
  自 2014年, 至 2014年
• 基盤研究(B)
  東南アジア・オセアニアにおけるオオコウモリ由来新興感染症の出現予測
  自 2014年, 至 2014年
• 基盤研究(B)
  東南アジア・オセアニアにおけるオオコウモリ由来新興感染症の出現予測
  自 2013年, 至 2013年
• 挑戦的萌芽研究
  低重力環境は次世代のゲノムと形態を変化させるか？
  自 2013年, 至 2013年

論文

• 紅藻を用いた「食べる」ワクチンの開発
  大松　勉
  アグリバイオ
  北隆館
  2024年08月, （MISC）総説・解説（商業誌）, 単独, 8, 9
• Low potential of persistence and dissemination of antimicrobial-resistant Enterobacterales by wild nutria (Myocastor coypus) in a local river of Gifu Prefecture
  Nakatsubo, Tomoki; Nakamura, Kohei; Omatsu, Tsutomu; Sugiyama, Michiyo; Asai, Tetsuo
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  . Environmental pollution caused by antimicrobial resistance is a global public health concern. To investigate the contribution of nutrias (Myocastor coypus) to the presence of extended-spectrum & beta;-lactamase (ESBL)-producing Enterobacterales in the Ijira River, prevalence of ESBL-producing Enterobacterales in their feces was examined using deoxycholate-hydrogen sulfide-lactose agar containing cefotaxime. Additionally, the composition of the fecal microbiota of nutria was examined using DNA metabarcoding analysis of the 16S ribosomal RNA gene and compared with that of Amami rabbit, deer, fox, and raccoon dog. The absence of ESBL-producing Enterobacterales and substantially lower abundance of Enterobacterales was observed in the feces of nutrias than in those of other wild mammals. Our results suggest the low potential of antimicrobialresistant Enterobacterales persistence and dissemination by nutria.
  2023年06月, 研究論文（学術雑誌）, 共同, 85, 6, 0916-7250, DOI(公開)(r-map), 613, 616
• Diverse Molecular Mechanisms Underlying Microbe-Inducing Male Killing in the Moth Homona magnanima
  Arai, Hiroshi; Takamatsu, Takumi; Lin, Shiou-Ruei; Mizutani, Tetsuya; Omatsu, Tsutomu; Katayama, Yukie; Nakai, Madoka; Kunimi, Yasuhisa; Inoue, Maki N.
  APPLIED AND ENVIRONMENTAL MICROBIOLOGY
  AMER SOC MICROBIOLOGY
  Many microbes induce male killing (MK) in various insect species. However, it is not well understood whether microbes adopt similar or different MK mechanisms. Male killing (MK) is a type of reproductive manipulation induced by microbes, where sons of infected mothers are killed during development. MK is a strategy that enhances the fitness of the microbes, and the underlying mechanisms and the process of their evolution have attracted substantial attention. Homona magnanima, a moth, harbors two embryonic MK bacteria, namely, Wolbachia (Alphaproteobacteria) and Spiroplasma (Mollicutes), and a larval MK virus, Osugoroshi virus (OGV; Partitiviridae). However, whether the three distantly related male killers employ similar or different mechanisms to accomplish MK remains unknown. Here, we clarified the differential effects of the three male killers on the sex-determination cascades and development of H. magnanima males. Reverse transcription-PCR demonstrated that Wolbachia and Spiroplasma, but not OGVs, disrupted the sex-determination cascade of males by inducing female-type splice variants of doublesex (dsx), a downstream regulator of the sex-determining gene cascade. We also found that MK microbes altered host transcriptomes in different manners; Wolbachia impaired the host dosage compensation system, whereas Spiroplasma and OGVs did not. Moreover, Wolbachia and Spiroplasma, but not OGVs, triggered abnormal apoptosis in male embryos. These findings suggest that distantly related microbes employ distinct machineries to kill males of the identical host species, which would be the outcome of the convergent evolution.IMPORTANCE Many microbes induce male killing (MK) in various insect species. However, it is not well understood whether microbes adopt similar or different MK mechanisms. This gap in our knowledge is partly because different insect models have been examined for each MK microbe. Here, we compared three taxonomically distinct male killers (i.e., Wolbachia, Spiroplasma, and a partiti-like virus) that infect the same host. We provided evidence that microbes can cause MK through distinct mechanisms that differ in the expression of genes involved in sex determination, dosage compensation, and apoptosis. These results imply independent evolutionary scenarios for the acquisition of their MK ability.
  2023年05月31日, 研究論文（学術雑誌）, 共同, 89, 5, 0099-2240, DOI(公開)(r-map)
• Isolation of extended-spectrum β-lactamase-producing Escherichia coli from Japanese red fox (Vulpes vulpes japonica)
  Tetsuo Asai, Michiyo Sugiyama, Tsutomu Omatsu, Masato Yoshikawa, Toshifumi Minamoto
  MicrobiologyOpen
  2022年10月, 研究論文（学術雑誌）, 共同, 11, 5, DOI(公開)(r-map)
• Establishment of an experimental model of normal dog bladder organoid using a three-dimensional culture method
  Elbadawy, Mohamed; Fujisaka, Kodai; Yamamoto, Haru; Tsunedomi, Ryouichi; Nagano, Hiroaki; Ayame, Hiromi; Ishihara, Yusuke; Mori, Takashi; Azakami, Daigo; Uchide, Tsuyoshi; Fukushima, Ryuji; Abugomaa, Amira; Kaneda, Masahiro; Yamawaki, Hideyuki; Shinohara, Yuta; Omatsu, Tsutomu; Mizutani, Tetsuya; Usui, Tatsuya; Sasaki, Kazuaki
  BIOMEDICINE & PHARMACOTHERAPY
  ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
  Dog bladder cancer (BC) is mostly muscle-invasive (MI) with poor prognosis, and its pathogenesis is close to human MIBC. Three-dimensional (3D) organoid culture ensures novel knowledge on cancer diseases including BC. Recently, we have established dog BC organoids (BCO) using their urine samples. BCO recapitulated the epithelial structures, characteristics, and drug sensitivity of BC-diseased dogs. However, organoids from dog normal bladder epithelium are not established yet. Therefore, the present study aimed to establish dog normal bladder organoids (NBO) for further understanding the pathogenesis of dog BC and human MIBC. The established NBO underwent various analyzes including cell marker expressions, histopathological structures, cancer-related gene expression patterns, and drug sensitivity. NBO could be produced non-invasively with a continuous culturing and recapitulated the structures and characteristics of the dog's normal bladder mucosal tissues. Different drug sensitivities were observed in each NBO. The analysis of RNA sequencing revealed that several novel genes were changed in NBO compared with BCO. NBO showed a higher expression of p53 and E-cadherin, but a lower expression of MDM2 and Twist1 compared with BCO. These results suggest that NBO could be a promising experimental 3D model for studying the developmental mechanisms of dog BC and human MIBC.
  2022年07月, 研究論文（学術雑誌）, 共同, 151, 0753-3322, DOI(公開)(r-map)
• Validation of the usefulness of 26S rDNA D1/D2, internal transcribed spacer, and intergenic spacer 1 for molecular epidemiological analysis of Macrorhabdus ornithogaster
  Kojima, Atsushi; Osawa, Nanako; Oba, Mami; Katayama, Yukie; Omatsu, Tsutomu; Mizutani, Tetsuya
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  Macrorhabdus ornithogaster (MO) is an infectious fungus that causes gastric damage in birds. In this study, we established nested and seminested polymerase chain reaction (PCR) methods that specifically amplify the domain D1/D2 region (D1/D2) of 26S ribosomal DNA (rDNA), internal transcribed spacer (ITS) of rDNA, and intergenic spacer (IGS) 1 region from avian feces. Phylogenetic analysis of MO collected from Japanese pet birds showed little genetic variation; analysis based on these regions did not distinguish between host species order, differences in MO shape, or host gastrointestinal symptoms. These regions were found to be unsuitable for molecular epidemiological studies of MO and further investigation into other genetic regions is required.
  2022年02月, 研究論文（学術雑誌）, 共同, 84, 2, 0916-7250, DOI(公開)(r-map), 244, 250
• African pygmy hedgehog adenovirus: Virus replication, virus-induced cytopathogenesis and activation of mitogen-activated protein kinase signaling pathways in infected MDCK cells
  Wen, Rongduo; Ochiai, Hideharu; Uchiyama, Jumpei; Osawa, Nanako; Oba, Mami; Katayama, Yukie; Li, Kaixin; Omatsu, Tsutomu; Tamukai, Kenichi; Suzuki, Kaoru; Madarame, Hiroo; Makino, Shinji; Mizutani, Tetsuya
  RESEARCH IN VETERINARY SCIENCE
  ELSEVIER SCI LTD
  We examined several aspects of African hedgehog adenovirus (AhAdv-1) that was isolated from an African pygmy hedgehog, including: replication kinetics of, virus-induced cytopathic effect (CPE), activation status of mitogen-activated protein kinase (MAPK) signaling pathways, and possible roles of these signaling pathways in virus replication and virus-induced CPE in MDCK cells. AhAdv-1 efficiently replicated and induced CPE in infected cells and caused accumulation of cleaved caspase-3 at 24 h post-infection (p.i.), suggesting apoptosis induction. Analysis of several intracellular signal transduction pathways, which are involved in apoptosis, showed activation of p38 MAPK, Akt and ERK1/2 pathways at 3 h p.i., and upregulation of phosphorylated SAPK/JNK at 24 h p.i. Although p38 MAPK inhibitor and SAPK/JNK inhibitor suppressed activation of the respective pathways in infected cells, they did not inhibit virus-induced CPE. Treatment of infected cells with inhibitor of the Akt pathway, the p38 pathway, the SAPK/JNK pathway or the ERK pathway revealed that inhibitors of p38 pathway inhibited viral replication by real-time PCR and TCID50 assay in infected MDCK cells, suggesting that AhAdv-1 uses p38 pathway for multiplication in infected cells.
  2021年10月, 研究論文（学術雑誌）, 共同, 139, 0034-5288, DOI(公開)(r-map), 152, 158
• Establishment of Intestinal Organoid from Rousettus leschenaultii and the Susceptibility to Bat-Associated Viruses, SARS-CoV-2 and Pteropine Orthoreovirus
  Elbadawy, Mohamed; Kato, Yuki; Saito, Nagisa; Hayashi, Kimika; Abugomaa, Amira; Kobayashi, Mio; Yoshida, Toshinori; Shibutani, Makoto; Kaneda, Masahiro; Yamawaki, Hideyuki; Mizutani, Tetsuya; Lim, Chang-Kweng; Saijo, Masayuki; Sasaki, Kazuaki; Usui, Tatsuya; Omatsu, Tsutomu
  INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
  MDPI
  Various pathogens, such as Ebola virus, Marburg virus, Nipah virus, Hendra virus, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle East Respiratory Syndrome Coronavirus (MERS-CoV), and SARS-CoV-2, are threatening human health worldwide. The natural hosts of these pathogens are thought to be bats. The rousette bat, a megabat, is thought to be a natural reservoir of filoviruses, including Ebola and Marburg viruses. Additionally, the rousette bat showed a transient infection in the experimental inoculation of SARS-CoV-2. In the current study, we established and characterized intestinal organoids from Leschenault's rousette, Rousettus leschenaultii. The established organoids successfully recapitulated the characteristics of intestinal epithelial structure and morphology, and the appropriate supplements necessary for long-term stable culture were identified. The organoid showed susceptibility to Pteropine orthoreovirus (PRV) but not to SARS-CoV-2 in experimental inoculation. This is the first report of the establishment of an expandable organoid culture system of the rousette bat intestinal organoid and its sensitivity to bat-associated viruses, PRV and SARS-CoV-2. This organoid is a useful tool for the elucidation of tolerance mechanisms of the emerging rousette bat-associated viruses such as Ebola and Marburg virus.
  2021年10月, 研究論文（学術雑誌）, 共同, 22, 19, DOI(公開)(r-map)
• Prevalence of antimicrobial resistance in bacteria isolated from Great Cormorants (Phalacrocorax carbo hanedae) in Japan
  Odoi, Justice Opare; Sugiyama, Michiyo; Kitamura, Yuko; Sudo, Akiko; Omatsu, Tsutomu; Asai, Tetsuo
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  Wild birds are recognized as disseminators of antimicrobial-resistant (AMR) bacteria into the environment. Here, we isolated AMR indicator bacteria from 198 Great Cormorant cloacal swabs collected in Shiga (n=90), Oita (n=52), Gifu (n=29), and Gunma (n=27) Prefectures, Japan, in 2018 and 2019. In total, 198 Aeromonas spp. and 194 Escherichia spp. were isolated, and their antimicrobial susceptibility was examined. Aeromonas spp. were resistant to colistin (8.6%), nalidixic acid (4%), and other antimicrobials (<2%), with 3.0% positivity for mcr-3. Escherichia spp. showed resistance to colistin (3.1%), ampicillin (2.6%), tetracycline (2.1%), and other antimicrobials (<2%). This study shows the presence of AMR bacteria in Great Cormorants, indicating that these birds potentially disseminate AMR bacteria.
  2021年08月, 研究論文（学術雑誌）, 共同, 83, 8, 0916-7250, DOI(公開)(r-map), 1191, 1195
• Viral-derived DNA invasion and individual variation in an Indonesian population of large flying fox Pteropus vampyrus
  Iida, Atsuo; Takemae, Hitoshi; Tarigan, Ronald; Kobayashi, Ryosuke; Kato, Hirokazu; Shimoda, Hiroshi; Omatsu, Tsutomu; Supratikno; Basri, Chaerul; Mayasari, Ni Luh Putu Ika; Agungpriyono, Srihadi; Maeda, Ken; Mizutani, Tetsuya; Hondo, Eiichi
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  Here, we performed next-generation sequencing (NGS) on six large flying foxes (Pteropus vampyrus) collected in Indonesia. Seventy-five virus species in the liver tissue of each specimen were listed. Viral homologous sequences in the bat genome were identified from the listed viruses. This finding provides collateral evidence of viral endogenization into the host genome. We found that two of the six specimens bore partial sequences that were homologous to the plant pathogens Geminiviridae and Luteoviridae. These sequences were absent in the P. vampyrus chromosomal sequences. Hence, plant viral homologous sequences were localized to the hepatocytes as extrachromosomal DNA fragments. Therefore, this suggests that the bat is a potential carrier or vector of plant viruses. The present investigation on wild animals offered novel perspectives on viral invasion, variation, and host interaction.
  2021年07月, 研究論文（学術雑誌）, 共同, 83, 7, 0916-7250, DOI(公開)(r-map), 1068, 1074
• Association between the bla(CTX-M-14)-harboring Escherichia coli Isolated from Weasels and Domestic Animals Reared on a University Campus
  Yossapol, Montira; Yamamoto, Miku; Sugiyama, Michiyo; Odoi, Justice Opare; Omatsu, Tsutomu; Mizutani, Tetsuya; Ohya, Kenji; Asai, Tetsuo
  ANTIBIOTICS-BASEL
  MDPI
  Antimicrobial-resistant (AMR) bacteria affect human and animal health worldwide. Here, CTX-M-14-producing Escherichia coli isolates were isolated from Siberian weasels (Mustela sibirica) that were captured on a veterinary campus. To clarify the source of bacteria in the weasels, we examined the domestic animals reared in seven facilities on the campus. Extended-spectrum beta-lactamase (ESBL)-producing E. coli were isolated on deoxycholate hydrogen sulfide lactose agar, containing cephalexin (50 mu g/mL) or cefotaxime (2 mu g/mL), and were characterized with antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE), replicon typing, and beta-lactamase typing analyses. Next-generation sequencing of the ESBL-encoding plasmids was also performed. CTX-M-14 producers isolated from both domestic animals and weasels were classified into six clusters with seven PFGE profiles. The PFGE and antimicrobial resistance profiles were characterized by the animal facility. All CTX-M-14 plasmids belonged to the IncI1 type with a similar size (98.9-99.3 kb), except for one plasmid that was 105.5 kb in length. The unweighted pair group method with arithmetic mean (UPGMA) revealed that the CTX-M-14 plasmid in the weasel isolates might have the same origin as the CTX-M-14 plasmid in the domestic animals. Our findings shed further light on the association of antimicrobial resistance between wild and domestic animals.
  2021年04月, 研究論文（学術雑誌）, 共同, 10, 4, 2079-6382, DOI(公開)(r-map)
• Metagenomic identification, sequencing, and genome analysis of porcine hepe-astroviruses (bastroviruses) in porcine feces in Japan
  Nagai, Makoto; Okabayashi, Tamaki; Akagami, Masataka; Matsuu, Aya; Fujimoto, Yoshikazu; Hashem, Md Abul; Mekata, Hirohisa; Nakao, Ryo; Matsuno, Keita; Katayama, Yukie; Oba, Mami; Omatsu, Tsutomu; Asai, Tetsuo; Nakagawa, Keisuke; Ito, Hiroshi; Madarame, Hiroo; Kawai, Kazuhiro; Ito, Toshihiro; Nonaka, Nariaki; Tsukiyama-Kohara, Kyoko; Inoshima, Yasuo; Mizutani, Tetsuya; Misawa, Naoaki
  INFECTION GENETICS AND EVOLUTION
  ELSEVIER
  Recently, hepe-astrovirus-like RNA viruses named bastroviruses (BastVs), have been found in human, pig, bat, and rat fecal samples. In this study, we determined nearly complete genome sequences of four BastVs in the feces of healthy pigs. Genetic characterization revealed that these porcine BastVs (PBastVs) and BastVs from other animals including humans, had the same genome organization, that is, they contained three predicted conserved domains of viral methyltransferase, RNA helicase, and RdRp in the nonstructural ORF1 and the astrovirus capsid domain in the structural ORF2. Phylogenetic analyses using RNA-dependent RNA polymerase and the capsid region revealed that PBastVs branched with bat and rat BastVs; however, the groups formed by each host were distantly related to human BastVs. Pairwise amino acid sequence comparison demonstrated that PBastVs shared 95.2-98.6% and 76.1-95.5% sequence identity among each other in the ORF1 and ORF2 regions, respectively; the sequence identities between PBastVs and BastVs from other animals were 21.4-42.5% and 9.1-20.6% in the ORF1 and ORF2 regions, respectively. This suggested that BastVs were derived from a common ancestor but evolved independently in each host population during a prolonged period. Putative recombination events were identified in the PBastV genome, suggesting that PBastVs gain sequence diversity and flexibility through recombination events. In an analysis of previously obtained metagenomic data, PBastV sequence reads were detected in 7.3% (23/315) of fecal samples from pigs indicating that PBastVs are distributed among pig populations in Japan.
  2021年03月, 研究論文（学術雑誌）, 共同, 88, 1567-1348, DOI(公開)(r-map)
• Two Novel Endornaviruses Co-infecting a Phytophthora Pathogen of Asparagus officinalis Modulate the Developmental Stages and Fungicide Sensitivities of the Host Oomycete
  Uchida, Keiko; Sakuta, Kohei; Ito, Aori; Takahashi, Yumi; Katayama, Yukie; Omatsu, Tsutomu; Mizutani, Tetsuya; Arie, Tsutomu; Komatsu, Ken; Fukuhara, Toshiyuki; Uematsu, Seiji; Okada, Ryo; Moriyama, Hiromitsu
  FRONTIERS IN MICROBIOLOGY
  FRONTIERS MEDIA SA
  Two novel endornaviruses, Phytophthora endornavirus 2 (PEV2) and Phytophthora endornavirus 3 (PEV3) were found in isolates of a Phytophthora pathogen of asparagus collected in Japan. A molecular phylogenetic analysis indicated that PEV2 and PEV3 belong to the genus Alphaendornavirus. The PEV2 and PEV3 genomes consist of 14,345 and 13,810 bp, and they contain single open reading frames of 4,640 and 4,603 codons, respectively. Their polyproteins contain the conserved domains of an RNA helicase, a UDP-glycosyltransferase, and an RNA-dependent RNA polymerase, which are conserved in other alphaendornaviruses. PEV2 is closely related to Brown algae endornavirus 2, whereas PEV3 is closely related to Phytophthora endornavirus 1 (PEV1), which infects a Phytophthora sp. specific to Douglas fir. PEV2 and PEV3 were detected at high titers in two original Phytophthora sp. isolates, and we found a sub-isolate with low titers of the viruses during subculture. We used the high- and low-titer isolates to evaluate the effects of the viruses on the growth, development, and fungicide sensitivities of the Phytophthora sp. host. The high-titer isolates produced smaller mycelial colonies and much higher numbers of zoosporangia than the low-titer isolate. These results suggest that PEV2 and PEV3 inhibited hyphal growth and stimulated zoosporangium formation. The high-titer isolates were more sensitive than the low-titer isolate to the fungicides benthiavalicarb-isopropyl, famoxadone, and chlorothalonil. In contrast, the high-titer isolates displayed lower sensitivity to the fungicide metalaxyl (an inhibitor of RNA polymerase I) when compared with the low-titer isolate. These results indicate that persistent infection with PEV2 and PEV3 may potentially affect the fungicide sensitivities of the host oomycete.
  2021年02月03日, 研究論文（学術雑誌）, 共同, 12, DOI(公開)(r-map)
• Mosquito-borne viruses, insect-specific flaviviruses (family Flaviviridae, genus Flavivirus), Banna virus (family Reoviridae, genus Seadornavirus), Bogor virus (unassigned member of family Permutotetraviridae), and alphamesoniviruses 2 and 3 isolated from Indonesian mosquitoes
  Supriyono; Kuwata, Ryusei; Torii, Shun; Shimoda, Hiroshi; Ishijima, Keita; Yonemitsu, Kenzo; Minami, Shohei; Kuroda, Yudai; Tatemoto, Kango; Ngo Thuy Bao Tran; Takano, Ai; Omatsu, Tsutomu; Mizutani, Tetsuya; Itokawa, Kentaro; Isawa, Haruhiko; Sawabe, Kyoko; Takasaki, Tomohiko; Yuliani, Dewi Maria; Abiyoga, Dimas; Hadi, Upik Kesumawati; Setiyono, Agus; Hondo, Eiichi; Agungpriyono, Srihadi; Maeda, Ken
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  Mosquitoes transmit many kinds of arboviruses (arthropod-borne viruses), and numerous arboviral diseases have become serious problems in Indonesia. In this study, we conducted surveillance of mosquito-borne viruses at several sites in Indonesia during 2016-2018 for risk assessment of arbovirus infection and analysis of virus biodiversity in mosquito populations. We collected 10,015 mosquitoes comprising at least 11 species from 4 genera. Major collected mosquito species were Culex quinquefasciatus, Aedes albopictus, Culex tritaeniorhynchus, Aedes aegypti, and Armigeres subalbatus. The collected mosquitoes were divided into 285 pools and used for virus isolation using two mammalian cell lines, Vero and BHK-21, and one mosquito cell line, C6/36. Seventy-two pools showed clear cytopathic effects only in C6/36 cells. Using RT-PCR and next-generation sequencing approaches, these isolates were identified as insect flaviviruses (family Flaviviridae, genus Flavivirus), Banna virus (family Reoviridae, genus Seadornavirus), new permutotetravirus (designed as Bogor virus) (family Permutotetraviridae, genus Alphapermutotetravirus), and alphamesoniviruses 2 and 3 (family Mesoniviridae, genus Alphamesonivirus). We believed that this large surveillance of mosquitoes and mosquito-borne viruses provides basic information for the prevention and control of emerging and re- emerging arboviral diseases.
  2020年07月, 研究論文（学術雑誌）, 共同, 82, 7, 0916-7250, DOI(公開)(r-map), 1030, 1041
• Molecular characterization of full genome sequences of Newcastle disease viruses circulating among vaccinated chickens in Egypt during 2011-2013
  Giang Thi Huong Tran; Sultan, Serageldeen; Osman, Nabila; Hassan, Mohamed Ismail; Hieu Van Dong; Tung Duy Dao; Omatsu, Tsutomu; Katayama, Yukie; Mizutani, Tetsuya; Takeda, Yohei; Ogawa, Haruko; Imai, Kunitoshi
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  Although intensive vaccination programs have been implemented, Newcastle disease (ND) outbreaks, accompanied by severe economic losses, are still reported in Egypt. The genetic characterization of ND virus (NDV) strains isolated from ND-vaccinated chicken flocks provides essential information for improving ND control strategies. Therefore, here, 38 NDV strains were isolated and identified from outbreaks among vaccinated flocks of broiler chickens located in the provinces of Qena, Luxor, and Aswan of Upper Egypt during 2011-2013. The investigated broiler chicken flocks (aged 28 to 40 days) had high mortality rates of up to 80%. All NDV isolates were genetically analyzed using next-generation DNA sequencing. From these isolates, 10 representative NDV strains were selected for further genetic analyses. Phylogenetic analysis of full-length coding genes revealed that the Egyptian NDV isolates belonged to a single sub-genotype, VII.1.1. These isolates were phylogenetically distant from the vaccine strains, including La Sota or Clone 30 (genotype II), which have been commonly used to vaccinate chicken flocks. Amino acid substitution K78R was observed in the neutralizing epitopes of the F proteins; whereas several mutations were found in the neutralizing epitopes of the hemagglutinin-neuraminidase proteins, notably, E347K. Overall, our results suggested that the occurrence of neutralizing epitope variants may be one of potential reasons for ND outbreaks. Further studies are needed to determine the protective effect of current vaccines against circulating virulent NDV strains.
  2020年06月, 研究論文（学術雑誌）, 共同, 82, 6, 0916-7250, DOI(公開)(r-map), 809, 816
• Bacterial and protozoan pathogens/symbionts in ticks infecting wild grasscutters (Thryonomys swinderianus) in Ghana
  Adenyo, Christopher; Ohya, Kenji; Qiu, Yongjin; Takashima, Yasuhiro; Ogawa, Hirohito; Matsumoto, Tateki; Thu, May June; Sato, Kozue; Kawabata, Hiroki; Katayama, Yukie; Omatsu, Tsutomu; Mizutani, Tetsuya; Fukushi, Hideto; Katakura, Ken; Nonaka, Narikaki; Inoue-Murayama, Miho; Kayang, Boniface; Nakao, Ryo
  ACTA TROPICA
  ELSEVIER
  Ticks and tick-borne pathogens constitute a great threat to livestock production and are a potential health hazard to humans. Grasscutters (Thryonomys swinderianus) are widely hunted for meat in Ghana and many other West and Central African countries. However, tick-borne zoonotic risks posed by wild grasscutters have not been assessed. The objective of this study was to investigate bacterial and protozoan pathogens in ticks infecting wild grasscutters. A total of 81 ticks were collected from three hunted grasscutters purchased from Kantamanto, the central bushmeat market in Accra. Ticks were identified as Ixodes aulacodi and Rhipicephalus sp. based on morphological keys, which were further confirmed by sequencing mitochondrial 16S ribosomal DNA (rDNA) and cytochrome oxidase I (COI) genes of specimens. Protozoan infections were tested by PCR amplifying 18S rDNA of Babesia/Theileria/Hepatozoon, while bacterial infections were evaluated by PCRs or real-time PCRs targeting Anaplasmataceae, Borrelia, spotted fever group rickettsiae, chlamydiae and Candidatus Midichloria mitochondrii. The results of PCR screening showed that 35.5% (27 out of 76) of I. aulacodi were positive for parasite infections. Sequencing analysis of the amplified products gave one identical sequence showing similarity with Babesia spp. reported from Africa. The Ca. M. mitochondrii endosymbiont was present in 85.5% (65 out of 76) of I. aulacodi but not in the five Rhipicephalus ticks. Two Anaplasmataceae bacteria genetically related to Ehrlichia muris and Anaplasma phagocytophilum were also detected in two I. aulacodi. None of the ticks were positive for Borrelia spp., spotted fever group rickettsiae and chlamydiae. Since I. aulacodi on wild grasscutters are potential carriers of tick-borne pathogens, some of which could be of zoonotic potential, rigorous tick control and pathogen analyses should be instituted especially when wild caught grasscutters are being used as foundation stock for breeding.
  2020年05月, 研究論文（学術雑誌）, 共同, 205, 0001-706X, DOI(公開)(r-map)
• Efficacy of primary liver organoid culture from different stages of non-alcoholic steatohepatitis (NASH) mouse model
  Elbadawy, Mohamed; Yamanaka, Megumi; Goto, Yuta; Hayashi, Kimika; Tsunedomi, Ryouichi; Hazama, Shoichi; Nagano, Hiroaki; Yoshida, Toshinori; Shibutani, Makoto; Ichikawa, Ryo; Nakahara, Junta; Omatsu, Tsutomu; Mizutani, Tetsuya; Katayama, Yukie; Shinohara, Yuta; Abugomaa, Amira; Kaneda, Masahiro; Yamawaki, Hideyuki; Usui, Tatsuya; Sasaki, Kazuaki
  BIOMATERIALS
  ELSEVIER SCI LTD
  Non-alcoholic steatohepatitis (NASH) is associated with liver fibrosis and cirrhosis, which eventually leads to hepatocellular carcinoma. Although several animal models were developed to understand the mechanisms of NASH pathogenesis and progression, it remains obscure. A 3D organoid culture system can recapitulate organ structures and maintain gene expression profiles of original tissues. We therefore tried to generate liver organoids from different degrees [defined as mild (NASH A), moderate (NASH B) and severe (NASH C)] of methionine- and choline-deficient diet-induced NASH model mice and analyzed the difference of their architecture, cell components, organoid-forming efficacy, and gene expression profiles. Organoids from each stage of NASH model mice were successfully generated. Interestingly, epithelial-mesenchymal transition was observed in NASH C organoids. Expression of Collagen I and an activated hepatic stellite cell marker, alpha-sma was upregulated in the liver organoids from NASH B and C mice. The analysis of RNA sequencing revealed that several novel genes were upregulated in all NASH liver organoids. These results suggest that our generated liver organoids from different stages of NASH diseased mice might become a useful tool for in vitro studies of the molecular mechanism of NASH development and also for identifying novel biomarkers for early diagnosis of NASH disease.
  2020年04月, 研究論文（学術雑誌）, 共同, 237, 0142-9612, DOI(公開)(r-map)
• Comparative histological studies on properties of polysaccharides secreted by vomeronasal glands of eight Laurasiatheria species
  Kondoh, Daisuke; Tomiyasu, Jumpei; Itakura, Raito; Sugahara, Mizuho; Yanagawa, Masashi; Watanabe, Kenichi; Alviola, Phillip A.; Yap, Sheryl A.; Cosico, Edison A.; Cruz, Florante A.; Larona, Ariel R.; Manalad, Allen J. F.; Masangkay, Joseph S.; Sugiura, Yuki; Kyuwa, Shigeru; Watanabe, Shumpei; Une, Yumi; Omatsu, Tsutomu; Bando, Hironori; Kato, Kentaro
  ACTA HISTOCHEMICA
  ELSEVIER GMBH
  Most mammalian species have a vomeronasal organ that detects specific chemical substances, such as pheromones. Mucous fluid covering the vomeronasal sensory epithelium is secreted by vomeronasal glands, and the properties of these fluids have been suggested to be involved in chemical detection. Histological studies using periodic acid-Schiff (PAS) and Alcian blue pH 2.5 (AB) stains, which respectively detect natural and acidic polysaccharides, have suggested variations in the nature of the vomeronasal glands among species. Here, we investigated the responsivity of the vomeronasal glands to PAS and AB stains in eight Laurasiatheria species. All species studied herein possessed vomeronasal glands that stained positive for PAS, like other many reported species. The vomeronasal glands of dogs and minks - like rodents, were AB-negative, whereas those of cows, goats, sika deer, musk shrews and two bat species were positive. Considering the present findings and previous reports, the vomeronasal glands in most of Laurasiatheria species appear to be fundamentally abundant in acidic polysaccharides, whereas those in carnivores essentially contains neutral polysaccharides.
  2020年04月, 研究論文（学術雑誌）, 共同, 122, 3, 0065-1281, DOI(公開)(r-map)
• Comparative Analysis of Fecal Microbiota in Grasscutter (Thryonomys swinderianus) and Other Herbivorous Livestock in Ghana
  Kawasaki, Kiyonori; Ohya, Kenji; Omatsu, Tsutomu; Katayama, Yukie; Takashima, Yasuhiro; Kinoshita, Tsuyoshi; Odoi, Justice Opare; Sawai, Kotaro; Fukushi, Hideto; Ogawa, Hirohito; Inoue-Murayama, Miho; Mizutani, Tetsuya; Adenyo, Christopher; Matsumoto, Yoshiki; Kayang, Boniface
  MICROORGANISMS
  MDPI
  The grasscutter (also known as the greater cane rat; Thryonomys swinderianus) is a large rodent native to West Africa that is currently under domestication process for meat production. However, little is known about the physiology of this species. In the present study, aiming to provide information about gut microbiota of the grasscutter and better understand its physiology, we investigated the intestinal microbiota of grasscutters and compared it with that of other livestock (cattle, goat, rabbit, and sheep) using 16S rRNA metagenomics analysis. Similar to the other herbivorous animals, bacteria classified as Bacteroidales, Clostridiales, Ruminococcaceae, and Lachnospiraceae were abundant in the microbiome of grasscutters. However, Prevotella and Treponema bacteria, which have fiber fermentation ability, were especially abundant in grasscutters, where the relative abundance of these genera was higher than that in the other animals. The presence of these genera might confer grasscutters the ability to easily breakdown dietary fibers. Diets for grasscutters should be made from ingredients not consumed by humans to avoid competition for resources and the ability to digest fibers may allow the use of fiber-rich feed materials not used by humans. Our findings serve as reference and support future studies on changes in the gut microbiota of the grasscutter as domestication progresses in order to establish appropriate feeding methods and captivity conditions.
  2020年02月, 研究論文（学術雑誌）, 共同, 8, 2, DOI(公開)(r-map)
• Prevalence of Agamid adenoviruses of the bearded dragons (Pogona vitticeps) in Japan
  Akabane, Yoshihito; Oba, Mami; Hata, Keisuke; Ochiai, Hideharu; Katayama, Yukie; Omatsu, Tsutomu; Okumura, Atsushi; Okumura, Maho; Madarame, Hiroo; Mizutani, Tetsuya
  JAPANESE JOURNAL OF VETERINARY RESEARCH
  HOKKAIDO UNIV
  In this study, we surveyed the prevalence and characteristics of agamid adenovirus (genus Atadenovirus) infections in bearded dragons (Pogona vitticeps) in Japan. Swab samples were collected from the oral cavity and pharynx of 44 healthy bearded dragons and 24 bearded dragons with clinical signs of respiratory disease. PCR confirmed agamid adenovirus in 25 of the 44 healthy lizards (56.8%). Of the 24 bearded dragons with respiratory clinical signs, 14 were agamid adenovirus-positive (58.3%). Sex was determined for 21 of the 24 bearded dragons with respiratory clinical signs (9 males and 12 females). Agamid adenovirus was confirmed in two of the nine males (22.2%) and 10 of the 12 females (83.3%), indicating a higher prevalence of adenovirus in the females. Overall, the prevalence of agamid adenovirus in bearded dragons with respiratory clinical signs was almost the same as that in clinically healthy bearded dragons, suggesting that the virus is widespread in this species. In addition, we detected no apparent seasonality in the occurrence of agamid adenovirus infection. The mean value of globulin was slightly higher in seven of the female lizards with confirmed agamid adenovirus.
  2020年02月, 研究論文（学術雑誌）, 共同, 68, 1, 0047-1917, DOI(公開)(r-map), 47, 53
• Development of a one-run real-time PCR detection system for pathogens associated with porcine respiratory diseases
  Sunaga, Fujiko; Tsuchiaka, Shinobu; Kishimoto, Mai; Aoki, Hiroshi; Kakinoki, Mari; Kure, Katsumasa; Okumura, Hanako; Okumura, Maho; Okumura, Atsushi; Nagai, Makoto; Omatsu, Tsutomu; Mizutani, Tetsuya
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  The etiology of Porcine respiratory disease complex is complicated by infections with multiple pathogens, and multiple infections increase the difficulty in identifying the causal pathogen. In this present study, we developed a detection system of microbes from porcine respiratory by using TaqMan real-time PCR (referred to as Dempo-PCR) to screen a broad range of pathogens associated with porcine respiratory diseases in a single run. We selected 17 porcine respiratory pathogens (Actinobacillus pleuropneumoniae, Boldetella bronchiseptica, Haemophilus parasuis, Pasteurella multocida, Pasteurella multocida toxin, Streptococcus suis, Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Mycoplasma hyosynovie, porcine circovirus 2, pseudorabies virus, porcine cytomegalovirus, swine influenza A virus, porcine reproductive and respiratory virus US strain, EU strain, porcine respiratory coronavirus and porcine hemagglutinating encephalomyelitis virus) as detection targets and designed novel specific primer-probe sets for seven of them. In sensitivity test by using standard curves from synthesized DNA, all primer-probe sets showed high sensitivity. However, porcine reproductive and respiratory virus is known to have a high frequency of genetic mutations, and the primer and probe sequences will need to be checked at a considerable frequency when performing Dempo-PCR from field samples. A total of 30 lung samples from swine showing respiratory symptoms on six farms were tested by the Dempo-PCR to validate the assay's clinical performance. As the results, 12 pathogens (5 virus and 7 bacteria) were detected and porcine reproductive and respiratory virus US strain, Mycoplasma hyorhinis, Haemophilus parasuis, and porcine cytomegalovirus were detected at high frequency. These results suggest that Dempo-PCR assay can be applied as a screening system with wide detection targets.
  2020年02月, 研究論文（学術雑誌）, 共同, 82, 2, 0916-7250, DOI(公開)(r-map), 217, 223
• Complete genome sequencing and genetic analysis of a Japanese porcine torovirus strain detected in swine feces
  Fujii, Yuki; Kashima, Yuki; Sunaga, Fujiko; Aoki, Hiroshi; Imai, Ryo; Sano, Kaori; Katayamas, Yukie; Omatsu, Tsutomu; Oba, Mami; Furuya, Tetsuya; Tsuzuku, Satoko; Ouchi, Yoshinao; Shirai, Junsuke; Mizutani, Tetsuya; Oka, Tomoichiro; Nagai, Makoto
  ARCHIVES OF VIROLOGY
  SPRINGER WIEN
  We sequenced the complete genome of a porcine torovirus (PToV) strain from Japan for the first time. Whole-genome analysis revealed that this strain (Iba/2018) has a mosaic sequence composed of at least three genome backgrounds, related to US, Chinese and German PToV strains. Clear recombination breakpoints were detected in the M and HE coding regions. A similarity plot and structural analysis demonstrated that the HE coding region exhibits the highest diversity, and the most sequence variation was found in the lectin domain. PToVs were divided into two lineages in the HE region, whereas clear lineages were not found in other regions.
  2020年02月, 研究論文（学術雑誌）, 共同, 165, 2, 0304-8608, DOI(公開)(r-map), 471, 477
• Genetic diversity of enterovirus G detected in faecal samples of wild boars in Japan: identification of novel genotypes carrying a papain-like cysteine protease sequence
  Nagata, Ayaka; Sekiguchi, Yuya; Oi, Toru; Sunaga, Fujiko; Madarame, Hiroo; Imai, Ryo; Sano, Kaori; Katayama, Yukie; Omatsu, Tsutomu; Oba, Mami; Furuya, Tetsuya; Shirai, Junsuke; Okabayashi, Tamaki; Misawa, Naoaki; Oka, Tomoichiro; Mizutani, Tetsuya; Nagai, Makoto
  JOURNAL OF GENERAL VIROLOGY
  MICROBIOLOGY SOC
  The genetic diversity of enterovirus G (EV-G) was investigated in the wild-boar population in Japan. EV-G-specific reverse transcription PCR demonstrated 30 (37.5%) positives out of 80 faecal samples. Of these, viral protein 1 (VP1) fragments of 20 samples were classified into G1 (3 samples), G4 (1 sample). G6 (2 samples), G8 (4 samples), G11 (1 sample). G12 (7 samples). G14 (1 sample) and G17 (1 sample), among which 11 samples had a papain-like cysteine protease (PL-CP) sequence, believed to be the first discoveries in 61 (2 samples) or G17 (1 sample) wild-boar EV-Gs, and in G8 (2 samples) or G12 (6 samples) EV-Gs from any animals. Sequences of the non-structural protein regions were similar among EV-Gs possessing the PL-CP sequence (PL-CP EV-Gs) regardless of genotype or origin, suggesting the existence of a common ancestor for these strains. Interestingly, for the two G8 and two G12 samples, the genome sequences contained two versions, with or without the PL-CP sequence. together with the homologous 2C/PL-CP and PL-CP/3A junction sequences, which may explain how the recombination and deletion of the PL-CP sequences occured in the PL-CP EV-G genomes. These findings shed light on the genetic plasticity and evolution of EV-G.
  2020年, 研究論文（学術雑誌）, 共同, 101, 8, 0022-1317, DOI(公開)(r-map), 840, 852
• END-phenomenon negative bovine viral diarrhea virus that induces the host's innate immune response supports propagation of BVDVs with different immunological properties
  Virology
  2019年12月, 研究論文（学術雑誌）, 共同, 538, DOI(公開)(r-map), 97, 110
• Complete genome sequencing and genetic characterization of porcine sapovirus genogroup (G) X and GXI: GVI, GVII, GX, and GXI sapoviruses share common genomic features and form a unique porcine SaV Glade
  Infection, Genetics and Evolution
  2019年11月01日, 研究論文（学術雑誌）, 共同, 75, DOI(公開)(r-map), 103959
• A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative antiapoptosis gene in place of viral structural protein genes
  Infection, Genetics and Evolution
  2019年11月01日, 研究論文（学術雑誌）, 共同, 75, DOI(公開)(r-map), 103975
• Molecular characterization of a virulent strain of Newcastle disease virus isolated from a diseased chicken in Kyrgyzstan in 2016
  JAPANESE JOURNAL OF VETERINARY RESEARCH
  2019年11月, 研究論文（学術雑誌）, 共同, 67, DOI(公開)(r-map), 263, 273
• First identification of Sapoviruses in wild boar
  Katsuta, Risako; Sunaga, Fujiko; Oi, Tom; Doan, Yen Hai; Tsuzuku, Satoko; Suzuki, Yoshihisa; Sano, Kaori; Katayama, Yukie; Omatsu, Tsutomu; Oba, Mami; Furuya, Tetsuya; Ouchi, Yoshinao; Shirai, Junsuke; Mizutani, Tetsuya; Oka, Tomoichiro; Nagai, Makoto
  VIRUS RESEARCH
  ELSEVIER
  Sapoviruses (Says) are enteric viruses that have been detected in human and animals previously; however, SaVs have not been identified in wild boar yet. Using a metagenomics approach, we identified SaVs in fecal samples of free-living wild boars in Japan for the first time. Six of the 48 specimens identified belonged to one genogroup (G)III, one GV and four GVI SaV sequence reads. We successfully determined complete genome of GV and GVI SaV strains using the long reverse transcription PCR strategy and the 5' rapid amplification of cDNA end method. Phylogenetic tree analysis and pairwise distance calculation revealed that GV SaV detected from wild boar was related to recently assigned GV.5 strains from pig, while GVI SaV was assigned to a new genotype within GVI. Moreover, wild boar may act as a reservoir for transmission of SaVs to the pig population (and vice versa) because GIII, GV, and GVI SaVs were all detected in pigs previously.
  2019年10月, 研究論文（学術雑誌）, 共同, 271, 0168-1702, DOI(公開)(r-map)
• Establishment of a novel experimental model for muscle-invasive bladder cancer using a dog bladder cancer organoid culture
  Elbadawy, Mohamed; Usui, Tatsuya; Mori, Takashi; Tsunedomi, Ryouichi; Hazama, Shoichi; Nabeta, Rina; Uchide, Tsuyoshi; Fukushima, Ryuji; Yoshida, Toshinori; Shibutani, Makoto; Tanaka, Takaharu; Masuda, Sosuke; Okada, Rena; Ichikawa, Ryo; Omatsu, Tsutomu; Mizutani, Tetsuya; Katayama, Yukie; Noguchi, Shunsuke; Iwai, Satomi; Nakagawa, Takayuki; Shinohara, Yuta; Kaneda, Masahiro; Yamawaki, Hideyuki; Sasaki, Kazuaki
  CANCER SCIENCE
  WILEY
  In human and dogs, bladder cancer (BC) is the most common neoplasm affecting the urinary tract. Dog BC resembles human muscle-invasive BC in histopathological characteristics and gene expression profiles, and could be an important research model for this disease. Cancer patient-derived organoid culture can recapitulate organ structures and maintains the gene expression profiles of original tumor tissues. In a previous study, we generated dog prostate cancer organoids using urine samples, however dog BC organoids had never been produced. Therefore we aimed to generate dog BC organoids using urine samples and check their histopathological characteristics, drug sensitivity, and gene expression profiles. Organoids from individual BC dogs were successfully generated, expressed urothelial cell markers (CK7, CK20, and UPK3A) and exhibited tumorigenesis in vivo. In a cell viability assay, the response to combined treatment with a range of anticancer drugs (cisplatin, vinblastine, gemcitabine or piroxicam) was markedly different in each BC organoid. In RNA-sequencing analysis, expression levels of basal cell markers (CK5 and DSG3) and several novel genes (MMP28, CTSE, CNN3, TFPI2, COL17A1, and AGPAT4) were upregulated in BC organoids compared with normal bladder tissues or two-dimensional (2D) BC cell lines. These established dog BC organoids might be a useful tool, not only to determine suitable chemotherapy for BC diseased dogs but also to identify novel biomarkers in human muscle-invasive BC. In the present study, for the 1st time, dog BC organoids were generated and several specifically upregulated organoid genes were identified. Our data suggest that dog BC organoids might become a new tool to provide fresh insights into both dog BC therapy and diagnostic biomarkers.
  2019年09月, 研究論文（学術雑誌）, 共同, 110, 9, 1347-9032, DOI(公開)(r-map), 2806, 2821
• Phylogenetic analysis of novel posaviruses detected in feces of Japanese pigs with posaviruses and posa-like viruses of vertebrates and invertebrates
  Aoki, Hiroshi; Sunaga, Fujiko; Ochiai, Hideharu; Masuda, Tsuneyuki; Ito, Mika; Akagami, Masataka; Naoi, Yuki; Sano, Kaori; Katayama, Yukie; Omatsu, Tsutomu; Oba, Mami; Sakaguchi, Shoichi; Furuya, Tetsuya; Ouchi, Yoshinao; Shirai, Junsuke; Mizutani, Tetsuya; Oka, Tomoichiro; Nagai, Makoto
  ARCHIVES OF VIROLOGY
  SPRINGER WIEN
  Posaviruses and posa-like viruses are unclassified viruses with sequence similarity to viruses of the order Picornavirales. They have been reported in various vertebrates and invertebrates. We identified 11 posavirus-like sequences in porcine feces and performed phylogenic analysis. Previously reported Japanese posaviruses and those identified in this study clustered with posavirus 1, 4, and 7 and husavirus 1, while five viruses branched into three independent lineages, tentatively named posavirus 10, 11, and 12. Interestingly, posaviruses, except for posavirus 8 and 9, husaviruses, and rasaviruses, formed a cluster consisting of viruses only from pigs, humans, and rats, while posavirus 8 and 9, fisavirus, and basaviruses clustered with posa-like viruses from invertebrates.
  2019年08月, 研究論文（学術雑誌）, 共同, 164, 8, 0304-8608, DOI(公開)(r-map), 2147, 2151
• Epidemic nodular facial myxomatous dermatitis in juvenile Cranwell's horned frogs Ceratophrys cranwelli.
  Diseases of aquatic organisms
  2019年04月, 共同, 134, 1, DOI(公開)(r-map), 57, 64
• Complete genomic analysis and molecular characterization of Japanese porcine sapeloviruses
  Sunaga, Fujiko; Masuda, Tsuneyuki; Ito, Mika; Akagami, Masataka; Naoi, Yuki; Sano, Kaori; Katayama, Yukie; Omatsu, Tsutomu; Oba, Mami; Sakaguchi, Shoichi; Furuya, Tetsuya; Yamasato, Hiroshi; Ouchi, Yoshinao; Shirai, Junsuke; Mizutani, Tetsuya; Nagai, Makoto
  VIRUS GENES
  SPRINGER
  The Porcine Sapelovirus (PSV) is an enteric virus of pigs that can cause various disorders. However, there are few reports that describe the molecular characteristics of the PSV genome. In this study, almost the entire genomes of 23 PSVs detected in Japanese pigs were analyzed using bioinformatics. Analysis of the cis-active RNA elements showed that the predicted secondary structures of the internal ribosome entry site in the 5 untranslated region (UTR) and a cis-replication element in the 2C coding region were conserved among PSVs. In contrast, those at the 3 UTR were different for different PSVs; however, tertiary structures between domains were conserved across all PSVs. Phylogenetic analysis of nucleotide sequences of the complete VP1 region showed that PSVs exhibited sequence diversity; however, they could not be grouped into genotypes due to the low bootstrap support of clusters. The insertion and/or deletion patterns in the C-terminal VP1 region were not related to the topology of the VP1 tree. The 3CD phylogenetic tree was topologically different from the VP1 tree, and PSVs from the same country were clustered independently. Recombination analysis revealed that recombination events were found upstream of the P2 region and some recombination breakpoints involved insertions and/or deletions in the C-terminal VP1 region. These findings demonstrate that PSVs show genetic diversity and frequent recombination events, particularly in the region upstream of the P2 region; however, PSVs could currently not be classified into genotypes and conserved genetic structural features of the cis-active RNA elements are observed across all PSVs.
  2019年04月, 研究論文（学術雑誌）, 共同, 55, 2, 0920-8569, DOI(公開)(r-map), 198, 208
• Encephalomyocarditis virus is potentially derived from eastern bent-wing bats living in East Asian countries
  Virus Research
  2019年01月02日, 研究論文（学術雑誌）, 共同, 2, 259, DOI(公開)(r-map), 62, 67
• Metagenomic identification and sequence analysis of a Teschovirus A-related virus in porcine feces in Japan, 2014-2016
  Oba, Mami; Naoi, Yuki; Ito, Mika; Masuda, Tsuneyuki; Katayama, Yukie; Sakaguchi, Shoichi; Omatsu, Tsutomu; Furuya, Tetsuya; Yamasato, Hiroshi; Sunaga, Fujiko; Makino, Shinji; Mizutani, Tetsuya; Nagai, Makoto
  INFECTION GENETICS AND EVOLUTION
  ELSEVIER SCIENCE BV
  Porcine Teschoviruses (PTVs) are associated with polioencephalomyelitis and various diseases, including reproductive and gastrointestinal disorders, of pigs and wild boars, and are also detected in the feces of healthy pigs. The genus Teschovirus contains a single species Teschovirus A that currently includes 13 serotypes. In the present study, we identified novel PTVs that are distantly related to Teschovirus A and were found in fecal samples of pigs with or without diarrhea in Japan. Phylogenetic analysis of amino acid (aa) sequences of the complete coding region revealed that these newly identified viruses did not cluster with any strains of PTVs or other strains within the picornavirus supergroup 1, suggesting that the viruses may not belong to Teschovirus A or any genus of the family Picornaviridae. These novel PTVs share a type IV internal ribosomal entry site and conserved characteristic motifs in the coding region, yet exhibit 62.2-79.0%, 86.6-92.8%, 77.1-81.0%, and 84.3-86.7% aa identities to PTV strains in P1, 2C, 3C, and 3D regions, respectively. In contrast, PTV 1-13 strains of the Teschovirus A share 76.5-92.1%, 88.1-99.7%, 93.2-100%, and 95.8-100% aa identities in the P1, 2C, 3C, and 3D, respectively, within the species. These data imply that the newly identified viruses belong to teschoviruses, and may represent a novel species in the genus Teschovirus.
  2018年12月, 研究論文（学術雑誌）, 共同, 66, 1567-1348, DOI(公開)(r-map), 210, 216
• Isolation of Pteropine orthoreovirus from Pteropus vampyrus in Garut, Indonesia
  Takemae, Hitoshi; Basri, Chaerul; Mayasari, Ni Luh Putu Ika; Tarigan, Ronald; Shimoda, Hiroshi; Omatsu, Tsutomu; Supratikno; Pramono, Didik; Cahyadi, Danang Dwi; Kobayashi, Ryosuke; Iida, Keisuke; Mizutani, Tetsuya; Maeda, Ken; Agungpriyono, Srihadi; Hondo, Eiichi
  VIRUS GENES
  SPRINGER
  Flying foxes belonging to the genus Pteropus are known to be reservoirs of zoonotic viruses. In this study, we describe the isolation of Pteropine orthoreovirus (PRV) from rectal swab samples of Pteropus vampyrus in Indonesia. PRV is an emerging zoonotic respiratory virus that can be transmitted from bats to humans. Rectal swabs (n=91) were screened by PCR for PRV and 10 (11%) were positive. Phylogenetic analysis based on nucleotide sequences indicated that the S2, S3, S4, M3, L2, and L3 segments of one isolate (Garut-69) were closely related to previously isolated strains in Indonesia. The remaining gene segments showed both similarity and genetic divergence with other PRV strains, suggesting that re-assortment events had occurred. This is the first report of PRV infection to P. vampyrus in West Java, Indonesia.
  2018年12月, 研究論文（学術雑誌）, 共同, 54, 6, 0920-8569, DOI(公開)(r-map), 823, 827
• Malaria knowledge, preventive actions, and treatment-seeking behavior among ethnic minorities in Ratanakiri Province, Cambodia: a community-based cross-sectional survey
  BMC Public Health.
  2018年10月26日, 共同, 18, 1, DOI(公開)(r-map)
• Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs
  Rahpaya, Sayed Samim; Tsuchiaka, Shinobu; Kishimoto, Mai; Oba, Mami; Katayama, Yukie; Nunomura, Yuka; Kokawa, Saki; Kimura, Takashi; Kobayashi, Atsushi; Kirino, Yumi; Okabayashi, Tamaki; Nonaka, Nariaki; Mekata, Hirohisa; Aoki, Hiroshi; Shiokawa, Mai; Umetsu, Moeko; Morita, Tatsushi; Hasebe, Ayako; Otsu, Keiko; Asai, Tetsuo; Yamaguchi, Tomohiro; Makino, Shinji; Murata, Yoshiteru; Abi, Ahmad Jan; Omatsu, Tsutomu; Mizutani, Tetsuya
  JOURNAL OF VETERINARY SCIENCE
  KOREAN SOC VETERINARY SCIENCE
  Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain reaction (PCR), which is based on TaqMan real-time PCR. Fifty-seven samples were positive for at least one pathogen, including bovine viral diarrhea virus, bovine enterovirus, Salmonella enterica ser. Dublin, Salmonella enterica ser. Typhimurium, and Neospora caninum; some samples were positive for multiple pathogens. Bovine viral diarrhea virus and bovine enterovirus were the most frequently detected pathogens, especially in flies, suggesting an important role of flies in the transmission of these viruses. Additionally, we detected the N. caninum genome from a cockroach sample for the first time. Our data suggest that insects (particularly flies), birds, and rodents are potential vectors and reservoirs of abortion, diarrhea, and respiratory infectious agents, and that they may transmit more than one pathogen at the same time.
  2018年05月, 研究論文（学術雑誌）, 共同, 19, 3, 1229-845X, DOI(公開)(r-map), 350, +
• An ascovirus isolated from Spodoptera litura (Noctuidae: Lepidoptera) transmitted by the generalist endoparasitoid Meteorus pulchricornis (Braconidae: Hymenoptera)
  Arai, Eiko; Ishii, Kazuo; Ishii, Hiroki; Sagawa, Shiori; Makiyama, Nao; Mizutani, Tetsuya; Omatsu, Tsutomu; Katayama, Yukie; Kunimi, Yasuhisa; Inoue, Maki N.; Nakai, Madoka
  JOURNAL OF GENERAL VIROLOGY
  MICROBIOLOGY SOC
  The family Ascoviridae is a recently described virus family whose members are transmitted by parasitoids and cause chronic and lethal infections in lepidopteran insects. Little is known about the biology and ecology of ascoviruses, and few isolates have been found outside the United States. We report here the isolation of a new ascovirus variant from Spodoptera litura in Japan. Full genome sequence and phylogenetic analyses showed that this virus was closely related to variants in Heliothis virescens ascovirus-3a, and it was named HvAV-3j. HvAV-3j has a DNA genome of 191 718 bp, with 189 putative ORFs and a GC content of 45.6 %, and is highly similar to HvAV-3h, which was isolated in China. In a field survey, the endoparasitoid Meteorus pulchricornis caused a high percentage of parasitization in populations of S. litura larvae, and under laboratory conditions M. pulchricornis was able to transmit HvAV-3j from infected to uninfected larvae by oviposition. Meteorus pulchricornis is thus likely to be a major vector for HvAV-3j transmission in Japan. This species is recognized here for the first time as a vector of ascoviruses that parasitizes a range of host species that extends across families.
  2018年04月, 研究論文（学術雑誌）, 共同, 99, 4, 0022-1317, DOI(公開)(r-map), 574, 584
• Molecular epidemiological survey and phylogenetic analysis of bovine influenza D virus in Japan
  Mekata, H.; Yamamoto, M.; Hamabe, S.; Tanaka, H.; Omatsu, T.; Mizutani, T.; Hause, B. M.; Okabayashi, T.
  TRANSBOUNDARY AND EMERGING DISEASES
  WILEY
  The influenza D virus, a new member of the Orthomyxoviridae family, is predominantly found in cattle. Although viral pathology and clinical disease in cattle appear mild, this virus plays an important role as a trigger of bovine respiratory disease (BRD). BRD is a costly illness worldwide. Thus, epidemiological surveys of the influenza D virus are necessary. Here, we conducted a molecular epidemiological survey for the influenza D virus in healthy and respiratory-diseased cattle in Japan. We found that 2.1% (8/377) of the cattle were infected with influenza D. The cattle with and without respiratory symptoms had approximately equal amounts of the virus. A full-genome sequence analysis revealed that the influenza D virus that was isolated in Japan formed an individual cluster that was distinct from the strains found in other countries. These results suggest that this virus might have evolved uniquely in Japan over a long period of time and that the viral pathology of Japanese strains might be different from the strains found in other countries. Continuous surveillance is required to determine the importance of this virus and to characterize its evolution.
  2018年04月, 研究論文（学術雑誌）, 共同, 65, 2, 1865-1674, DOI(公開)(r-map), e355, e360
• Barriers for pregnant women living in rural, agricultural villages to accessing antenatal care in Cambodia: A community-based cross-sectional study combined with a geographic information system
  Yasuoka, Junko; Nanishi, Keiko; Kikuchi, Kimiyo; Suzuki, Sumihiro; Ly, Po; Thavrin, Boukheng; Omatsu, Tsutomu; Mizutani, Tetsuya
  PLOS ONE
  PUBLIC LIBRARY SCIENCE
  Background Maternal morbidity and mortality is still a major public health issue in low-and middle-income countries such as Cambodia. Improving access to antenatal care (ANC) services for pregnant women has been widely recognized as one of the most effective means of reducing maternal mortality and morbidity. As such, this study examined the barriers for pregnant women living in rural, agricultural villages to accessing ANC based on data collected in the Ratanakiri province, one of the least developed provinces in Cambodia, using a combination of a community-based cross-sectional survey and a geographic information system (GIS). Methods A community-based cross-sectional survey was conducted among 377 mothers with children under the age of two living in 62 villages in the Ratanakiri province, Cambodia, in December 2015. Face-to-face interviews were conducted to ask mothers about their ANC service use, knowledge of ANC, barriers to accessing health facilities, and complications they experienced during the most recent pregnancy. At the same time, GIS data were also collected using a Global Positioning System (GPS) to accurately measure actual travel distance of pregnant women to access health facilities and to examine geographical and environmental barriers in greater detail. Results Only a third of the mothers met the recommendations made by the World Health Organization (WHO) of receiving ANC four times or more (achieved ANC4+), and a quarter of the mothers had never received ANC during their most recent pregnancy. Factors positively associated with achieving ANC4+ were mother's secondary or higher education (adjusted odds ratio [AOR] = 5.50, 95% confidence interval [CI]: 1.74, 17.37), being aware that receiving ANC is recommended (AOR = 2.74, 95% CI: 1.25, 6.00), and knowledge about the recommended frequency for ANC (AOR = 2.26, 95% CI: 7.22). Actual travel distance was negatively associated with achieving ANC4+. Mothers who had to travel 10.0 +/- 14.9 km were 68% less likely (AOR = 0.32, 95% CI: 0.10, 0.99), and those who had to travel 15.0 km or longer were 79% less likely (AOR = 0.21, 95% CI: 0.07, 0.62) to have achieved ANC 4+, both compared to those who travelled 5.0 km or less. While most previous studies have used a straight-line to measure distance traveled, this study much more accurately measured the actual distance traveled by using a GIS. As a result, there was a statistically significant discrepancy between actual travel distance and straight-line distance. Conclusions This study revealed promoting factors and barriers for ANC use among pregnant women living in remote, agricultural villages in Cambodia. Furthermore, this study highlights the importance of measuring travel distances accurately to ensure that targeted interventions for ANC are not misguided by straight-line distances. The methodology used in this study can be applied widely to other developing countries, especially in remote areas with limited road networks where there may be a large discrepancy between actual and straight-line distances.
  2018年03月19日, 研究論文（学術雑誌）, 共同, 13, 3, 1932-6203, DOI(公開)(r-map)
• Discovery of genome of an immunodeficiency associated virus-like virus from pig feces in Japan
  Oba, Mami; Katayama, Yukie; Tsuchiaka, Shinobu; Omatsu, Tsutomu; Murata, Yoshiteru; Ohya, Kenji; Makino, Shinji; Nagai, Makoto; Mizutani, Tetsuya
  JAPANESE JOURNAL OF VETERINARY RESEARCH
  HOKKAIDO UNIV
  Immunodeficiency-associated stool virus (IASV) is an unclassified virus, for which the only genome information is available from a patient infected by human immunodeficiency virus. In domestic animals, one report described detection of IASV-like virus in pig feces, whereas no nucleotide sequence information of this virus is currently available. Using deep sequencing method, we detected a DNA fragment homologous to IASV in several pig feces in Japan. The sequence of the PCR product in this sample had 70% homology to that of IASV. The infectious rate of the IASV-like virus was 72.9% among the 9 pig farms, from which the samples were collected. There was no clear correlation between the presence of IASV-like virus and the fecal characteristics.
  2018年02月, 研究論文（学術雑誌）, 共同, 66, 1, 0047-1917, DOI(公開)(r-map), 53, 56
• Decreased expression of the immediate early protein, ICP4, by deletion of the tegument protein VP22 of equine herpesvirus type 1
  Okada, Ayaka; Suganuma, Shota; Badr, Yassien; Omatsu, Tsutomu; Mizutani, Tetsuya; Ohya, Kenji; Fukushi, Hideto
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  VP22 is a major tegument protein of equine herpesvirus type 1 (EHV-1). In the present study, we examined functions of VP22 in EHV-1 replication by viral protein expression analyses in cells infected with the VP22-deficient virus. The expressions of several viral proteins in the cells infected with the VP22-deficient virus were lower than those in the cells infected with the parent virus. One of the weakly expressed proteins was identified as ICP4, which is a major regulatory protein encoded by an immediate early gene of EHV-1. A real-time PCR analysis showed that the mRNA expression of ICP4 was the same in cells infected with the parent and VP22-deficient viruses. Hence, VP22 appears to promote synthesis of ICP4 post-transcriptionally.
  2018年02月, 研究論文（学術雑誌）, 共同, 80, 2, 0916-7250, DOI(公開)(r-map), 311, 315
• Genetic diversity and recombination of enterovirus G strains in Japanese pigs: High prevalence of strains carrying a papain-like cysteine protease sequence in the enterovirus G population
  Tsuchiaka, Shinobu; Naoi, Yuki; Imai, Ryo; Masuda, Tsuneyuki; Ito, Mika; Akagami, Masataka; Ouchi, Yoshinao; Ishii, Kazuo; Sakaguchi, Shoichi; Omatsu, Tsutomu; Katayama, Yukie; Oba, Mami; Shirai, Junsuke; Satani, Yuki; Takashima, Yasuhiro; Taniguchi, Yuji; Takasu, Masaki; Madarame, Hiroo; Sunaga, Fujiko; Aoki, Hiroshi; Makino, Shinji; Mizutani, Tetsuya; Nagai, Makoto
  PLOS ONE
  PUBLIC LIBRARY SCIENCE
  To study the genetic diversity of enterovirus G (EV-G) among Japanese pigs, metagenomics sequencing was performed on fecal samples from pigs with or without diarrhea, collected between 2014 and 2016. Fifty-nine EV-G sequences, which were >5,000 nucleotides long, were obtained. By complete VP1 sequence analysis, Japanese EV-G isolates were classified into G1 (17 strains), G2 (four strains), G3 (22 strains), G4 (two strains), G6 (two strains), G9 (six strains), G10 (five strains), and a new genotype (one strain). Remarkably, 16 G1 and one G2 strain identified in diarrheic (23.5%; four strains) or normal (76.5%; 13 strains) fecal samples possessed a papain-like cysteine protease (PL-CP) sequence, which was recently found in the USA and Belgium in the EV-G genome, at the 2C +/- 3A junction site. This paper presents the first report of the high prevalence of viruses carrying PL-CP in the EV-G population. Furthermore, possible inter-and intragenotype recombination events were found among EV-G strains, including G1-PL-CP strains. Our findings may advance the understanding of the molecular epidemiology and genetic evolution of EV-Gs.
  2018年01月11日, 研究論文（学術雑誌）, 共同, 13, 1, 1932-6203, DOI(公開)(r-map)
• A new comprehensive method for detection of livestock-related pathogenic viruses using a target enrichment system
  Oba, Mami; Tsuchiaka, Shinobu; Omatsu, Tsutomu; Katayama, Yukie; Otomaru, Konosuke; Hirata, Teppei; Aoki, Hiroshi; Murata, Yoshiteru; Makino, Shinji; Nagai, Makoto; Mizutani, Tetsuya
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  ACADEMIC PRESS INC ELSEVIER SCIENCE
  We tested usefulness of a target enrichment system SureSelect, a comprehensive viral nucleic acid detection method, for rapid identification of viral pathogens in feces samples of cattle, pigs and goats. This system enriches nucleic acids of target viruses in clinical/field samples by using a library of biotinylated RNAs with sequences complementary to the target viruses. The enriched nucleic acids are amplified by PCR and subjected to next generation sequencing to identify the target viruses. In many samples, SureSelect target enrichment method increased efficiencies for detection of the viruses listed in the biotinylated RNA library. Furthermore, this method enabled us to determine nearly full-length genome sequence of porcine parainfluenza virus 1 and greatly increased Breadth, a value indicating the ratio of the mapping consensus length in the reference genome, in pig samples. Our data showed usefulness of SureSelect target enrichment system for comprehensive analysis of genomic information of various viruses in field samples. (C) 2017 Elsevier Inc. All rights reserved.
  2018年01月08日, 研究論文（学術雑誌）, 共同, 495, 2, 0006-291X, DOI(公開)(r-map), 1871, 1877
• Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan
  Kuroda, Moegi; Masuda, Tsuneyuki; Ito, Mika; Naoi, Yuki; Doan, Yen Hai; Haga, Kei; Tsuchiaka, Shinobu; Kishimoto, Mai; Sano, Kaori; Omatsu, Tsutomu; Katayama, Yukie; Oba, Mami; Aoki, Hiroshi; Ichimaru, Toru; Sunaga, Fujiko; Mukono, Itsuro; Yamasato, Hiroshi; Shirai, Junsuke; Katayama, Kazuhiko; Mizutani, Tetsuya; Oka, Tomoichiro; Nagai, Makoto
  INFECTION GENETICS AND EVOLUTION
  ELSEVIER SCIENCE BV
  Sapoviruses (SaV) are enteric viruses infecting humans and animals. SaVs are highly diverse and are divided into multiple genogroups based on structural protein (VP1) sequences. SaVs detected from pigs belong to eight genogroups (GIII, GV, GVI, GVII, GVIII, GIX, GX, and GXI), but little is known about the SaV genogroup distribution in the Japanese pig population. In the present study, 26 nearly complete genome (> 6000 nucleotide: nt) and three partial sequences (2429 nt, 4364 nt, and 4419 nt in length, including the entire VP1 coding region) of SaV were obtained from one diarrheic and 15 non-diarrheic porcine feces in Japan via a metagenomics approach. Phylogenetic analysis of the complete VP1 amino acid sequence (aa) revealed that 29 porcine SaVs were classified into seven genogroups; GIII (11 strains), GV (1 strain), GVI (3 strains), GVII (6 strains), GVIII (1 strain), GX (3 strains), and GXI (4 strains). This manuscript presents the first nearly complete genome sequences of GX and GXI, and demonstrates novel intergenogroup recombination events.
  2017年11月, 研究論文（学術雑誌）, 共同, 55, 1567-1348, DOI(公開)(r-map), 209, 217
• Discovery of fur seal feces-associated circular DNA virus in swine feces in Japan
  Oba, Mami; Katayama, Yukie; Naoi, Yuki; Tsuchiaka, Shinobu; Omatsu, Tsutomu; Okumura, Atsushi; Nagai, Makoto; Mizutani, Tetsuya
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  Fur seal feces-associated circular ssDNA virus (FSfaCV) was discovered in a pig for the first time in Japan using a next-generation sequencer with duplex-specific nuclease. Full genome of the virus showed approximately 92% similarity to FSfaCVs from New Zealand fur seals. Furthermore, we investigated the prevalence of the ssDNA virus in 85 piglets in Japan, and 65 piglets were positive (76%) for the virus.
  2017年10月, 研究論文（学術雑誌）, 共同, 79, 10, 0916-7250, DOI(公開)(r-map), 1664, 1666
• Generation of a novel live rabies vaccine strain with a high level of safety by introducing attenuating mutations in the nucleoprotein and glycoprotein
  Nakagawa, Keisuke; Nakagawa, Kento; Omatsu, Tsutomu; Katayama, Yukie; Oba, Mami; Mitake, Hiromichi; Okada, Kazuma; Yamaoka, SatOko; Takashima, Yasuhiro; Masatani, Tatsunori; Okadera, Kota; Ito, Naoto; Mizutani, Tetsuya; Sugiyama, Makoto
  VACCINE
  ELSEVIER SCI LTD
  The current live rabies vaccine SAG2 is attenuated by only one mutation (Arg-to-Glu) at position 333 in the glycoprotein (G333). This fact generates a potential risk of the emergence of a pathogenic revertant by a back mutation at this position during viral propagation in the body. To circumvent this risk, it is desirable to generate a live vaccine strain highly and stably attenuated by multiple mutations. However, the information on attenuating mutations other than that at G333 is very limited. We previously reported that amino acids at positions 273 and 394 in the nucleoprotein (N273/394) (Leu and His, respectively) of fixed rabies virus Ni-CE are responsible for the attenuated phenotype by enhancing interferon (IFN)/chemokine gene expressions in infected neural cells. In this study, we found that amino acid substitutions at N273/394 (Phe-to-Leu and Tyr-to-His, respectively) attenuated the pathogenicity of the oral live vaccine ERA, which has a virulent-type Arg at G333. Then we generated ERA-N273/394-G333 attenuated by the combination of the above attenuating mutations at G333 and N273/394, and checked its safety. Similar to the ERA-G333, which is attenuated by only the mutation at G333, ERA-N273/394-G333 did not cause any symptoms in adult mice after intracerebral inoculation, indicating a low level of residual pathogenicity of ERA-N273/394-G333. Further examination revealed that infection with ERA N273/394-G333 induces IFN-beta and CXCL10 mRNA expressions more strongly than ERA-G333 infection in a neuroblastoma cell line. Importantly, we found that the ERA-N273/394-G333 stain has a lower risk for emergence of a pathogenic revertant than does the ERA-G333. These results indicate that ERA N273/394-G333 has a potential to be a promising candidate for a live rabies vaccine strain with a high level of safety. (C) 2017 Elsevier Ltd. All rights reserved.
  2017年10月, 研究論文（学術雑誌）, 共同, 35, 42, 0264-410X, DOI(公開)(r-map), 5622, 5628
• Molecular characteristics and prevalence of small ruminant lentiviruses in goats in Japan
  Kokawa, Saki; Oba, Mami; Hirata, Teppei; Tamaki, Shiro; Omura, Miki; Tsuchiaka, Shinobu; Nagai, Makoto; Omatsu, Tsutomu; Mizutani, Tetsuya
  ARCHIVES OF VIROLOGY
  SPRINGER WIEN
  Small ruminant lentiviruses (SRLVs), which comprise caprine arthritis-encephalitis virus (CAEV) and maedi-visna virus (MVV), are prevalent in goats and sheep worldwide, including in Japan. However, little is known about the molecular characteristics of goat lentiviruses in Japan. In this study, a molecular and phylogenetic analysis of the long gag region was performed. The phylogenic tree demonstrated that all samples belonged to SRLV subtype B1. Two clusters were identified, with one cluster distinct from previously reported strains of subtype B1. In addition, several alterations in the amino acid sequence were detected in immunodominant epitopes of the gag region. To gain a deeper understanding of the genetic diversity of SRLVs in Japan, it will be necessary to increase the sample size and conduct a broader survey. The present report is important for establishing baseline information on the prevalence of SRLV in Japan and providing data to develop a new, more sensitive diagnostic test for effective control of SRLV.
  2017年10月, 研究論文（学術雑誌）, 共同, 162, 10, 0304-8608, DOI(公開)(r-map), 3007, 3015
• Complete genome analysis of porcine kobuviruses from the feces of pigs in Japan
  Akagami, Masataka; Ito, Mika; Niira, Kazutaka; Kuroda, Moegi; Masuda, Tsuneyuki; Haga, Kei; Tsuchiaka, Shinobu; Naoi, Yuki; Kishimoto, Mai; Sano, Kaori; Omatsu, Tsutomu; Aoki, Hiroshi; Katayama, Yukie; Oba, Mami; Oka, Tomoichiro; Ichimaru, Toru; Yamasato, Hiroshi; Ouchi, Yoshinao; Shirai, Junsuke; Katayama, Kazuhiko; Mizutani, Tetsuya; Nagai, Makoto
  VIRUS GENES
  SPRINGER
  Porcine kobuviruses (PoKoVs) are ubiquitously distributed in pig populations worldwide and are thought to be enteric viruses in swine. Although PoKoVs have been detected in pigs in Japan, no complete genome data for Japanese PoKoVs are available. In the present study, 24 nearly complete or complete sequences of the PoKoV genome obtained from 10 diarrheic feces and 14 non-diarrheic feces of Japanese pigs were analyzed using a metagenomics approach. Japanese PoKoVs shared 85.2-100% identity with the complete coding nucleotide (nt) sequences and the closest relationship of 85.1-98.3% with PoKoVs from other countries. Twenty of 24 Japanese PoKoVs carried a deletion of 90 nt in the 2B coding region. Phylogenetic tree analyses revealed that PoKoVs were not grouped according to their geographical region of origin and the phylogenetic trees of the L, P1, P2, and P3 genetic regions showed topologies different from each other. Similarity plot analysis using strains from a single farm revealed partially different similarity patterns among strains from identical farm origins, suggesting that recombination events had occurred. These results indicate that various PoKoV strains are prevalent and not restricted geographically on pig farms worldwide and the coexistence of multiple strains leads to recombination events of PoKoVs and contributes to the genetic diversity and evolution of PoKoVs.
  2017年08月, 研究論文（学術雑誌）, 共同, 53, 4, 0920-8569, DOI(公開)(r-map), 593, 602
• Characterization of cross-clade monoclonal antibodies against H5N1 highly pathogenic avian influenza virus and their application to the antigenic analysis of diverse H5 subtype viruses
  Gronsang, Dulyatad; Bui, Anh N.; Trinh, Dai Q.; Bui, Vuong N.; Nguyen, Khong V.; Can, Minh X.; Omatsu, Tsutomu; Mizutani, Tetsuya; Nagai, Makoto; Katayama, Yukie; Thampaisarn, Rapeewan; Ogawa, Haruko; Imai, Kunitoshi
  ARCHIVES OF VIROLOGY
  SPRINGER WIEN
  H5N1 highly pathogenic avian influenza viruses (HPAIVs) are a threat to both animal and public health and require specific and rapid detection for prompt disease control. We produced three neutralizing anti-hemagglutinin (HA) monoclonal antibodies (mAbs) using two clades (2.2 and 2.5) of the H5N1 HPAIV isolated in Japan. Blocking immunofluorescence tests showed that each mAb recognized different epitopes; 3B5.1 and 3B5.2 mAbs against the clade 2.5 virus showed cross-clade reactivity to all 26 strains from clades 1, 2.2, 2.3.2.1, 2.3.2.1a, b, c and 2.3.4, suggesting that the epitope(s) recognized are conserved. Conversely, the 1G5 mAb against the clade 2.2 virus showed reactivity to only clades 1, 2.3.4 and 2.5 strains. An analysis of escape mutants, and some clades of the H5N1 viruses recognized by 3B5.1 and 3B5.2 mAbs, suggested that the mAbs bind to an epitope, including amino acid residues at position 162 in the HA1 protein (R162 and K162). Unexpectedly, however, when five Eurasian-origin H5 low-pathogenic AIV (LPAIV) strains with R162 were examined (EA-nonGsGD clade) as well as two American-origin strains (Am-nonGsGD clade), the mAb recognized only EA-nonGsGD clade strains. The R162 and K162 residues in the HA1 protein were highly conserved among 36 of the 43 H5N1 clades reported, including clades 2.3.2.1a and 2.3.2.1c that are currently circulating in Asia, Africa and Europe. The amino acid residues (158-PTIKRSYNNTNQE-170) in the HA1 protein are probably an epitope responsible for the cross-clade reactivity of the mAbs, considering the epitopes reported elsewhere. The 3B5.1 and 3B5.2 mAbs may be useful for the specific detection of H5N1 HPAIVs circulating in the field.
  2017年08月, 研究論文（学術雑誌）, 共同, 162, 8, 0304-8608, DOI(公開)(r-map), 2257, 2269
• First isolation and characterization of pteropine orthoreoviruses in fruit bats in the Philippines
  Taniguchi, Satoshi; Maeda, Ken; Horimoto, Taisuke; Masangkay, Joseph S.; Puentespina, Roberto, Jr.; Alvarez, James; Eres, Eduardo; Cosico, Edison; Nagata, Noriyo; Egawa, Kazutaka; Singh, Harpal; Fukuma, Aiko; Yoshikawa, Tomoki; Tani, Hideki; Fukushi, Shuetsu; Tsuchiaka, Shinobu; Omatsu, Tsutomu; Mizutani, Tetsuya; Une, Yumi; Yoshikawa, Yasuhiro; Shimojima, Masayuki; Saijo, Masayuki; Kyuwa, Shigeru
  ARCHIVES OF VIROLOGY
  SPRINGER WIEN
  Pteropine orthoreovirus (PRV) causes respiratory tract illness (RTI) in humans. PRVs were isolated from throat swabs collected from 9 of 91 wild bats captured on the Mindanao Islands, The Philippines, in 2013. The nucleic acid sequence of the whole genome of each of these isolates was determined. Phylogenetic analysis based on predicted amino acid sequences indicated that the isolated PRVs were novel strains in which re-assortment events had occurred in the viral genome. Serum specimens collected from 76 of 84 bats were positive for PRV-neutralizing antibodies suggesting a high prevalence of PRV in wild bats in the Philippines. The bat-borne PRVs isolated in the Philippines were characterized in comparison to an Indonesian PRV isolate, Miyazaki-Bali/2007 strain, recovered from a human patient, revealing that the Philippine bat-borne PRVs had similar characteristics in terms of antigenicity to those of the Miyazaki-Bali/2007 strain, but with a slight difference (e.g., growth capacity in vitro). The impact of the Philippine bat-borne PRVs should be studied in human RTI cases in the Philippines.
  2017年06月, 研究論文（学術雑誌）, 共同, 162, 6, 0304-8608, DOI(公開)(r-map), 1529, 1539
• Whole genome analysis of porcine astroviruses detected in Japanese pigs reveals genetic diversity and possible intra-genotypic recombination
  Ito, Mika; Kuroda, Moegi; Masuda, Tsuneyuki; Akagami, Masataka; Haga, Kei; Tsuchiaka, Shinobu; Kishimoto, Mai; Naoi, Yuki; Sano, Kaori; Omatsu, Tsutomu; Katayama, Yukie; Oba, Mami; Aoki, Hiroshi; Ichimaru, Toru; Mukono, Itsuro; Ouchi, Yoshinao; Yamasato, Hiroshi; Shirai, Junsuke; Katayama, Kazuhiko; Mizutani, Tetsuya; Nagai, Makoto
  INFECTION GENETICS AND EVOLUTION
  ELSEVIER SCIENCE BV
  Porcine astroviruses (PoAstVs) are ubiquitous enteric virus of pigs that are distributed in several countries throughout the world. Since PoAstVs are detected in apparent healthy pigs, the clinical significance of infection is unknown. However, AstVs have recently been associated with a severe neurological disorder in animals, including humans, and zoonotic potential has been suggested. To date, little is known about the epidemiology of PoAstVs among the pig population in Japan. In this report, we present an analysis of nearly complete genomes of 36 PoAstVs detected by a metagenomics approach in the feces of Japanese pigs. Based on a phylogenetic analysis and pairwise sequence comparison, 10, 5,15, and 6 sequences were classified as PoAstV2, PoAstV3, PoAstV4, and PoAstV5, respectively. Co-infection with two or three strains was found in individual fecal samples from eight pigs. The phylogenetic trees of ORF1a, ORF1b, and ORF2 of PoAstV2 and PoAstV4 showed differences in their topologies. The PoAstV3 and PoAstV5 strains shared high sequence identities within each genotype in all ORFs; however, one PoAstV3 strain and one PoAstV5 strain showed considerable sequence divergence from the other PoAstV3 and PoAstV5 strains, respectively, in ORF2. Recombination analysis using whole genomes revealed evidence of multiple possible intra-genotype recombination events in PoAstV2 and PoAstV4, suggesting that recombination might have contributed to the genetic diversity and played an important role in the evolution of Japanese PoAstVs. (C) 2017 Elsevier B.V. All rights reserved.
  2017年06月, 研究論文（学術雑誌）, 共同, 50, 1567-1348, DOI(公開)(r-map), 38, 48
• Mycobacterium avium subsp hominissuis menigoencephalitis in a cat
  Madarame, Hiroo; Saito, Miyoko; Ogihara, Kikumi; Ochiai, Hideharu; Oba, Mami; Omatsu, Tsutomu; Tsuyuki, Yuzo; Mizutani, Tetsuya
  VETERINARY MICROBIOLOGY
  ELSEVIER SCIENCE BV
  A 33-month old, neutered female Abyssinian cat died. The cat had sudden onset of widespread neurologic signs about half a year after birth. Mycobacterium avium subsp. hominissuis (MAH) group was isolated and identified from the brain of a cat affected with pyogranulomatous meningoencephalitis. The central nervous system (CNS) was involved in the disseminated MAH infection. MAH infection should be considered in cats with neurologic signs in regard to zoonotic aspects. Comparatively, this is a first case of MAH infection observed in the brain in either humans or animals.
  2017年05月, 研究論文（学術雑誌）, 共同, 204, 0378-1135, DOI(公開)(r-map), 43, 45
• Diversity in VP3, NSP3, and NSP4 of rotavirus B detected from Japanese cattle
  Hayashi-Miyamoto, Michiko; Murakami, Toshiaki; Minami-Fukuda, Fujiko; Tsuchiaka, Shinobu; Kishimoto, Mai; Sano, Kaori; Naoi, Yuki; Asano, Keigo; Ichimaru, Toru; Haga, Kei; Omatsu, Tsutomu; Katayama, Yukie; Oba, Mami; Aoki, Hiroshi; Shirai, Junsuke; Ishida, Motohiko; Katayama, Kazuhiko; Mizutani, Tetsuya; Nagai, Makoto
  INFECTION GENETICS AND EVOLUTION
  ELSEVIER SCIENCE BV
  Bovine rotavirus B (RVB) is an etiological agent of diarrhea mostly in adult cattle. Currently, a few sequences of viral protein (VP) 1, 2, 4, 6, and 7 and nonstructural protein (NSP) 1, 2, and 5 of bovine RVB are available in the DDBJ/EMBL/GenBank databases, and none have been reported for VP3, NSP3, and NSP4. In order to fill this gap in the genetic characterization of bovine RVB strains, we used a metagenomics approach and sequenced and analyzed the complete coding sequences (CDS) of VP3, NSP3, andNSP4 genes, aswell as the partial or complete CDS of other genes of RVBs detected fromJapanese cattle. VP3, NSP3, and NSP4 of bovine RVBs shared lownucleotide sequence identities (63.3-64.9% for VP3, 65.9-68.2% for NSP3, and 52.6-56.2% for NSP4) with those of murine, human, and porcine RVBs, suggesting that bovine RVBs belong to a novel genotype. Furthermore, significantly low amino acid sequence identities were observed for NSP4 (36.1-39.3%) between bovine RVBs and the RVBs of other species. In contrast, hydrophobic plot analysis of NSP4 revealed profiles similar to those of RVBs of other species and rotavirus A (RVA) strains. Phylogenetic analyses of all gene segments revealed that bovine RVB strains formed a cluster that branched distantly from other RVBs. These results suggest that bovine RVBs have evolved independently fromother RVBs but in a similar manner to other rotaviruses. These findings provide insights into the evolution and diversity of RVB strains. (C) 2017 Elsevier B. V. All rights reserved.
  2017年04月, 研究論文（学術雑誌）, 共同, 49, 1567-1348, DOI(公開)(r-map), 97, 103
• Development of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex
  Kishimoto, Mai; Tsuchiaka, Shinobu; Rahpaya, Sayed Samim; Hasebe, Ayako; Otsu, Keiko; Sugimura, Satoshi; Kobayashi, Suguru; Komatsu, Natsumi; Nagai, Makoto; Omatsu, Tsutomu; Naoi, Yuki; Sano, Kaori; Okazaki-Terashima, Sachiko; Oba, Mami; Katayama, Yukie; Sato, Reiichiro; Asai, Tetsuo; Mizutani, Tetsuya
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  Bovine respiratory disease complex (BRDC) is frequently found in cattle worldwide. The etiology of BRDC is complicated by infections with multiple pathogens, making identification of the causal pathogen difficult. Here, we developed a detection system by applying TaqMan real-time PCR (Dembo respiratory-PCR) to screen a broad range of microbes associated with BRDC in a single run. We selected 16 bovine respiratory pathogens (bovine viral diarrhea virus, bovine coronavirus, bovine parainfluenza virus 3, bovine respiratory syncytial virus, influenza D virus, bovine rhinitis A virus, bovine rhinitis B virus, bovine herpesvirus 1, bovine adenovirus 3, bovine adenovirus 7, Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, Trueperella I pyogenes, Mycoplasma bovis and Ureaplasma diversum) as detection targets and designed novel specific primer-probe sets for nine of them. The assay performance was assessed using standard curves from synthesized DNA. In addition, the sensitivity of the assay was evaluated by spiking solutions extracted from nasal swabs that were negative by Dembo respiratory-PCR for nucleic acids of pathogens or synthesized DNA. All primer-probe sets showed high sensitivity. In this study, a total of 40 nasal swab samples from cattle on six farms were tested by Dembo respiratory-PCR. Dembo respiratory-PCR can be applied as a screening system with wide detection targets.
  2017年03月, 研究論文（学術雑誌）, 共同, 79, 3, 0916-7250, DOI(公開)(r-map), 517, 523
• The full genome sequences of 8 equine herpesvirus type 4 isolates from horses in Japan
  Izume, Satoko; Kirisawa, Rikio; Ohya, Kenji; Ohnuma, Aiko; Kimura, Takashi; Omatsu, Tsutomu; Katayama, Yukie; Mizutani, Tetsuya; Fukushi, Hideto
  JOURNAL OF VETERINARY MEDICAL SCIENCE
  JAPAN SOC VET SCI
  Equine herpesvirus type 4 (EHV-4) is one of the most important pathogens in horses. To clarify the key genes of the EHV-4 genome that cause abortion in female horses, we determined the whole genome sequences of a laboratory strain and 7 Japanese EHV-4 isolates that were isolated from 2 aborted fetuses and nasal swabs of 5 horses with respiratory disease. The full genome sequences and predicted amino acid sequences of each gene of these isolates were compared with of the reference EHV-4 strain NS80567 and Australian isolates that were reported in 2015. The EHV-4 isolates clustered in 2 groups which did not reflect their pathogenicity. A comparison of the predicted amino acid sequences of the genes did not reveal any genes that were associated with EHV-4-induced abortion.
  2017年01月, 研究論文（学術雑誌）, 共同, 79, 1, 0916-7250, DOI(公開)(r-map), 206, 212
• Identification of a novel bovine enterovirus possessing highly divergent amino acid sequences in capsid protein
  Tsuchiaka, Shinobu; Rahpaya, Sayed Samim; Otomaru, Konosuke; Aoki, Hiroshi; Kishimoto, Mai; Naoi, Yuki; Omatsu, Tsutomu; Sano, Kaori; Okazaki-Terashima, Sachiko; Katayama, Yukie; Oba, Mami; Nagai, Makoto; Mizutani, Tetsuya
  BMC MICROBIOLOGY
  BIOMED CENTRAL LTD
  Background: Bovine enterovirus (BEV) belongs to the species Enterovirus E or F, genus Enterovirus and family Picornaviridae. Although numerous studies have identified BEVs in the feces of cattle with diarrhea, the pathogenicity of BEVs remains unclear. Previously, we reported the detection of novel kobu-like virus in calf feces, by metagenomics analysis. In the present study, we identified a novel BEV in diarrheal feces collected for that survey. Complete genome sequences were determined by deep sequencing in feces. Secondary RNA structure analysis of the 5' untranslated region (UTR), phylogenetic tree construction and pairwise identity analysis were conducted. Results: The complete genome sequences of BEV were genetically distant from other EVs and the VP1 coding region contained novel and unique amino acid sequences. We named this strain as BEV AN12/Bos taurus/JPN/2014 (referred to as BEV-AN12). According to genome analysis, the genome length of this virus is 7414 nucleotides excluding the poly (A) tail and its genome consists of a 5' UTR, open reading frame encoding a single polyprotein, and 3'UTR. The results of secondary RNA structure analysis showed that in the 5' UTR, BEV-AN12 had an additional clover leaf structure and small stem loop structure, similarly to other BEVs. In pairwise identity analysis, BEV-AN12 showed high amino acid (aa) identities to Enterovirus F in the polyprotein, P2 and P3 regions (aa identity >= 82.4%). Therefore, BEV-AN12 is closely related to Enterovirus F. However, aa sequences in the capsid protein regions, particularly the VP1 encoding region, showed significantly low aa identity to other viruses in genus Enterovirus (VP1 aa identity <= 58.6%). In addition, BEV-AN12 branched separately from Enterovirus E and F in phylogenetic trees based on the aa sequences of P1 and VP1, although it clustered with Enterovirus F in trees based on sequences in the P2 and P3 genome region. Conclusions: We identified novel BEV possessing highly divergent aa sequences in the VP1 coding region in Japan. According to species definition, we proposed naming this strain as ”Enterovirus K”, which is a novel species within genus Enterovirus. Further genomic studies are needed to understand the pathogenicity of BEVs.
  2017年01月, 研究論文（学術雑誌）, 共同, 17, 1471-2180, DOI(公開)(r-map)
• Characterization of avian paramyxovirus serotype 14, a novel serotype, isolated from a duck fecal sample in Japan
  Thampaisarn, Rapeewan; Bui, Vuong N.; Trinh, Dai Q.; Nagai, Makoto; Mizutani, Tetsuya; Omatsu, Tsutomu; Katayama, Yukie; Gronsang, Dulyatad; Le, Duong H. T.; Ogawa, Haruko; Imai, Kunitoshi
  VIRUS RESEARCH
  ELSEVIER SCIENCE BV
  A hemagglutinating virus isolate designated 11OG0352, was obtained from a duck fecal sample. Genetic and virological analyses indicated that it might represent a novel serotype of avian paramyxovirus (APMV). Electron micrographs showed that the morphology of the virus particle was similar to that of APMV. The complete genome of this virus comprised 15,444 nucleotides complying with the paramyxovirus ”rule of six” and contains six open reading frames (3'-N-P-M-F-HN-L-5'). The phylogenetic analysis of the whole genome revealed that the virus was a member of the genus Avulavirus, but that it was distinct from APMV-1 to APMV-13. Although the F-protein cleavage site was TREGK down arrow L, which resembles a lentogenic strain of APMV-1, the K residue at position-1 of the cleavage site was first discovered in APMV members. The phosphoprotein gene of isolate 11OG0352 contains a putative RNA editing site, 3'-AUUUUCCC-5' (negative sense) which sequence differs from that of other APMVs. The intracerebral pathogenicity index test did not detect virulence in infected chicks. In hemagglutination inhibition (HI) tests, an antiserum against this virus did not detectably react with other APMVs (serotypes 1-4, 6-9) except for low reciprocal cross-reactivity with APMV-6. We designated this isolate, as APMV-14/duck/Japan/11OG0352/2011 and propose that it is a novel APMV serotype. The HI test may not be widely applicable for the classification of a new serotype because of the limited availability of reference antisera against all serotypes and cross-reactivity data. The nucleotide sequence identities of the whole genome of 11OG0352 and other APMVs ranged from 46.3% to 56.1%. Such comparison may provide a useful tool for classifying new APMV isolates. However, the nucleotide sequence identity between APMV-12 and APMV-13 was higher (64%), which was nearly identical to the lowest nucleotide identity (67%) reported in subgroups within the serotype. Therefore, consensus criteria for using whole genome analysis should be established. (C) 2016 Elsevier B.V. All rights reserved.
  2017年01月, 研究論文（学術雑誌）, 共同, 228, 0168-1702, DOI(公開)(r-map), 46, 57
• Characterization of avian paramyxovirus serotype 14, a novel serotype, isolated from a duck fecal sample in Japan.
  Thampaisarn R, Bui VN, Trinh DQ, Nagai M, Mizutani T, Omatsu T, Katayama Y, Gronsang D, Le DHT, Ogawa H, Imai K
  Virus Res
  2017年01月, 研究論文（学術雑誌）, 共同, 228, 46, 57
• Identification of further diversity among posaviruses.
  Sano K, Naoi Y, Kishimoto M, Masuda T, Tanabe H, Ito M, Niira K, Haga K, Asano K, Tsuchiaka S, Omatsu T, Furuya T, Katayama Y, Oba M, Ouchi Y, Yamasato H, Ishida M, Shirai J, Katayama K, Mizutani T, Nagai M
  Arch Virol
  2016年12月, 研究論文（学術雑誌）, 共同, 161, 12, 3541, 3548
• Molecular phylogeny of a genetically divergent hantavirus harbored by the Geoffroy's rousette (Rousettus amplexicaudatus), a frugivorous bat species in the Philippines.
  Arai S, Taniguchi S, Aoki K, Yoshikawa Y, Kyuwa S, Tanaka-Taya K, Masangkay JS, Omatsu T, Puentespina R Jr, Watanabe S, Alviola P, Alvarez J, Eres E, Cosico E, Quibod MNRM, Morikawa S, Yanagihara R, Oishi K
  Infect Genet Evol
  2016年11月, 研究論文（学術雑誌）, 共同, 45, 26, 32
• Whole genome sequences of Japanese porcine species C rotaviruses reveal a high diversity of genotypes of individual genes and will contribute to a comprehensive, generally accepted classification system.
  Niira K, Ito M, Masuda T, Saitou T, Abe T, Komoto S, Sato M, Yamasato H, Kishimoto M, Naoi Y, Sano K, Tuchiaka S, Okada T, Omatsu T, Furuya T, Aoki H, Katayama Y, Oba M, Shirai J, Taniguchi K, Mizutani T, Nagai M
  Infect Genet Evol
  2016年10月, 研究論文（学術雑誌）, 共同, 44, 106, 113
• Genome sequence of a novel mitovirus identified in the phytopathogenic fungus Alternaria arborescens.
  Komatsu K, Katayama Y, Omatsu T, Mizutani T, Fukuhara T, Kodama M, Arie T, Teraoka T, Moriyama H
  Arch Virol
  2016年09月, 研究論文（学術雑誌）, 共同, 161, 9, 2627, 2631
• Detection of Campylobacter jejuni in rectal swab samples from Rousettus amplexicaudatus in the Philippines.
  Hatta Y, Omatsu T, Tsuchiaka S, Katayama Y, Taniguchi S, Masangkay JS, Puentespina R Jr, Eres E, Cosico E, Une Y, Yoshikawa Y, Maeda K, Kyuwa S, Mizutani T
  J Vet Med Sci
  2016年09月, 研究論文（学術雑誌）, 共同, 78, 8, 1347, 1350
• Characterization of Venom and Oviduct Components of Parasitoid Wasp Asobara japonica.
  Furihata S, Matsumura T, Hirata M, Mizutani T, Nagata N, Kataoka M, Katayama Y, Omatsu T, Matsumoto H, Hayakawa Y
  PLoS One
  2016年07月, 研究論文（学術雑誌）, 共同, 11, 7, e0160210
• Characterization and phylogenetic analysis of a novel picornavirus from swine feces in Japan.
  Naoi Y, Kishimoto M, Masuda T, Ito M, Tsuchiaka S, Sano K, Yamasato H, Omatsu T, Aoki H, Furuya T, Katayama Y, Oba M, Okada T, Shirai J, Mizutani T, Nagai M
  Arch Virol
  2016年06月, 研究論文（学術雑誌）, 共同, 161, 6, 1685, 1690
• Genome sequence of a novel victorivirus identified in the phytopathogenic fungus Alternaria arborescens.
  Komatsu K, Katayama Y, Omatsu T, Mizutani T, Fukuhara T, Kodama M, Arie T, Teraoka T, Moriyama H
  Arch Virol
  2016年06月, 研究論文（学術雑誌）, 共同, 161, 6, 1701, 1704
• Detection of Japanese eel endothelial cells-infecting virus in Anguilla japonica elvers.
  Okazaki S, Yasumoto S, Koyama S, Tsuchiaka S, Naoi Y, Omatsu T, Ono S, Mizutani T
  J Vet Med Sci
  2016年03月, 研究論文（学術雑誌）, 共同, 78, 4, 705, 707
• Detection and molecular characterization of Cryptosporidium and Eimeria species in Philippine bats.
  Murakoshi F, Recuenco FC, Omatsu T, Sano K, Taniguchi S, Masangkay JS, Alviola P, Eres E, Cosico E, Alvarez J, Une Y, Kyuwa S, Sugiura Y, Kato K
  Parasitol Res
  2016年03月, 研究論文（学術雑誌）, 共同, 115, 5, 1863, 1869
• Whole genome analysis of Japanese bovine toroviruses reveals natural recombination between porcine and bovine toroviruses.
  Ito M, Tsuchiaka S, Naoi Y, Otomaru K, Sato M, Masuda T, Haga K, Oka T, Yamasato H, Omatsu T, Sugimura S, Aoki H, Furuya T, Katayama Y, Oba M, Shirai J, Katayama K, Mizutani T, Nagai M
  Infect Genet Evol
  2016年03月, 研究論文（学術雑誌）, 共同, 38, 90, 95
• Development of a novel detection system for microbes from bovine diarrhea by real-time PCR.
  Tsuchiaka S, Masuda T, Sugimura S, Kobayashi S, Komatsu N, Nagai M, Omatsu T, Furuya T, Oba M, Katayama Y, Kanda S, Yokoyama T, Mizutani T
  J Vet Med Sci
  2016年03月, 研究論文（学術雑誌）, 共同, 78, 3, 383, 389
• A novel Bunyavirus from the soft tick, Argas vespertilionis, in Japan.
  Oba M, Omatsu T, Takano A, Fujita H, Sato K, Nakamoto A, Takahashi M, Takada N, Kawabata H, Ando S, Mizutani T.
  J Vet Med Sci
  2016年03月, 研究論文（学術雑誌）, 共同, 78, 3, 443, 445
• Involvement of the 3' Untranslated Region in Encapsidation of the Hepatitis C Virus.
  Shi G, Ando T, Suzuki R, Matsuda M, Nakashima K, Ito M, Omatsu T, Oba M, Ochiai H, Kato T, Mizutani T, Sawasaki T, Wakita T, Suzuki T.
  PLoS Pathog.
  2016年02月, 研究論文（学術雑誌）, 共同, 12, 2, e1005441
• Detection of novel kobu-like viruses in Japanese black cattle in Japan.
  Otomaru K, Naoi Y, Haga K, Omatsu T, Uto T, Koizumi M, Masuda T, Yamasato H, Takai H, Aoki H, Tsuchiaka S, Sano K, Okazaki S, Katayama Y, Oba M, Furuya T, Shirai J, Katayama K, Mizutani T, Nagai M.
  J Vet Med Sci.
  2016年02月, 研究論文（学術雑誌）, 共同, 78, 2, 321, 324
• Development of one-step real-time reverse transcriptase-PCR-based assays for the rapid and simultaneous detection of four viruses causing porcine diarrhea.
  Masuda T, Tsuchiaka S, Ashiba T, Yamasato H, Fukunari K, Omatsu T, Furuya T, Shirai J, Mizutani T, Nagai M.
  Jpn J Vet Res.
  2016年02月, 研究論文（学術雑誌）, 共同, 64, 1, 5, 14
• Quantitative PCR detection of feline morbillivirus in cat urine samples.
  Furuya T, Wachi A, Sassa Y, Omatsu T, Nagai M, Fukushima R, Shibutani M, Yamaguchi T, Uematsu Y, Shirota K, Mizutani T.
  J Vet Med Sci.
  2016年01月, 研究論文（学術雑誌）, 共同, 77, 12, 1701, 1703
• Identification and complete genome analysis of a novel bovine picornavirus in Japan.
  Nagai M, Omatsu T, Aoki H, Kaku Y, Belsham GJ, Haga K, Naoi Y, Sano K, Umetsu M, Shiokawa M, Tsuchiaka S, Furuya T, Okazaki S, Katayama Y, Oba M, Shirai J, Katayama K, Mizutani T.
  Virus Res.
  2015年12月, 研究論文（学術雑誌）, 共同, 210, 205, 212
• Complete Genome Sequences of Two Japanese Eel Endothelial Cell-Infecting Virus Strains Isolated in Japan.
  Naoi Y, Okazaki S, Katayama Y, Omatsu T, Ono S, Mizutani T.
  Genome Announc.
  2015年11月, 研究論文（学術雑誌）, 共同, 3, 6, e01236, 15
• Full genome analysis of bovine astrovirus from fecal samples of cattle in Japan: identification of possible interspecies transmission of bovine astrovirus.
  Nagai M, Omatsu T, Aoki H, Otomaru K, Uto T, Koizumi M, Minami-Fukuda F, Takai H, Murakami T, Masuda T, Yamasato H, Shiokawa M, Tsuchiaka S, Naoi Y, Sano K, Okazaki S, Katayama Y, Oba M, Furuya T, Shirai J, Mizutani T.
  Arch Virol.
  2015年10月, 研究論文（学術雑誌）, 共同, 160, 10, 2491, 2501
• Parrot bornavirus-2 and -4 RNA detected in wild bird samples in Japan are phylogenetically adjacent to those found in pet birds in Japan.
  Sassa Y, Bui VN, Saitoh K, Watanabe Y, Koyama S, Endoh D, Horie M, Tomonaga K, Furuya T, Nagai M, Omatsu T, Imai K, Ogawa H, Mizutani T.
  Virus Genes.
  2015年10月, 研究論文（学術雑誌）, 共同, 51, 2, 234, 43
• Isolation and characterization of a novel Rhabdovirus from a wild boar (Sus scrofa) in Japan.
  Sakai K, Hagiwara K, Omatsu T, Hamasaki C, Kuwata R, Shimoda H, Suzuki K, Endoh D, Nagata N, Nagai M, Katayama Y, Oba M, Kurane I, Saijo M, Morikawa S, Mizutani T, Maeda K.
  Vet Microbiol.
  2015年09月, 研究論文（学術雑誌）, 共同, 179, 3-4, 197, 203
• Use of S1 nuclease in deep sequencing for detection of double-stranded RNA viruses.
  Shimada S, Nagai M, Moriyama H, Fukuhara T, Koyama S, Omatsu T, Furuya T, Shirai J, Mizutani T.
  J Vet Med Sci.
  2015年09月, 研究論文（学術雑誌）, 共同, 77, 9, 1163, 1166
• Genetic and antigenic analysis of Chlamydia pecorum strains isolated from calves with diarrhea.
  Ohtani A, Kubo M, Shimoda H, Ohya K, Iribe T, Ohishi D, Endoh D, Omatsu T, Mizutani T, Fukushi H, Maeda K.
  J Vet Med Sci.
  2015年07月, 研究論文（学術雑誌）, 共同, 77, 7, 777, 782
• Whole-genome sequence analysis of G3 and G14 equine group A rotaviruses isolated in the late 1990s and 2009-2010.
  Nemoto M, Nagai M, Tsunemitsu H, Omatsu T, Furuya T, Shirai J, Kondo T, Fujii Y, Todaka R, Katayama K, Mizutani T.
  Arch Virol.
  2015年05月, 研究論文（学術雑誌）, 共同, 160, 5, 1171, 1179
• H2 genotypes of G4P[6], G5P[7], and G9[23] porcine rotaviruses show super-short RNA electropherotypes.
  Nagai M, Shimada S, Fujii Y, Moriyama H, Oba M, Katayama Y, Tsuchiaka S, Okazaki S, Omatsu T, Furuya T, Koyama S, Shirai J, Katayama K, Mizutani T.
  Vet Microbiol.
  2015年04月, 研究論文（学術雑誌）, 共同, 176, 3-4, 250, 256
• Two types of genetic carrier, the IncP genomic island and the novel IncP-1β plasmid, for the aac(2')-IIa gene that confers kasugamycin resistance in Acidovorax avenae ssp. avenae.
  Yoshii A, Omatsu T, Katayama Y, Koyama S, Mizutani T, Moriyama H, Fukuhara T.
  Mol Plant Pathol.
  2015年04月, 研究論文（学術雑誌）, 共同, 16, 3, 288, 300
• Detection of a pneumonia virus of mice (PVM) in an African hedgehog (Atelerix arbiventris) with suspected wobbly hedgehog syndrome (WHS).
  Madarame H, Ogihara K, Kimura M, Nagai M, Omatsu T, Ochiai H, Mizutani T.
  Vet Microbiol.
  2014年09月, 研究論文（学術雑誌）, 共同, 173, 1-2, 136, 140
• Epidemiology of Elephant Endotheliotropic Herpesivirus 1 in Elephants in Japanese Zoos.
  Taniguchi M, Oba M, Okazaki S, Omatsu T, Teshima Y, Shiina O, Kinjou T, Azama K, Shimabukuro Y, Fukuhara M, Nakamoto H, Sochi C, Kiryu Y, Miyake I, Fujino M, Masatou H, Iida S, Shinohara H, Oouchi I, Takahara K, Kimura S, Nakamoto T, Nishiura N, Tsuchiaka S, Katayama Y, Sassa Y, Nagai M, Furuya T, Kawakami S, Mizutani T
  Jour. Jpn. Assoc. Zoo. Aqu.
  2014年08月, 研究論文（学術雑誌）, 共同, 55, 2
• Stability analysis of mathematical model of Caprine arthritis encephalitis
  T Hirata, Y Yonahara, F Asharif, S Tamaki, T Omatsu, T Mizutani
  American Journal of Theoretical and Applied Statistics
  2014年06月, 研究論文（学術雑誌）, 共同, 3, 3, 73, 80
• Estimation of Parameters related to SFTSV (Severe Febrile Thrombocytopenia Syndrome Virus) Transmission of Sheep in Shandong, China
  Hirata T, Kinjyo Y, Nishihira Y, Yonahara Y, Miyagi T, Omatsu T, Shiroma Y, Mizutani Y, Nagata Y, Tamaki S
  Science Journal of Public Health
  2014年06月, 研究論文（学術雑誌）, 共同, 2, 3, 177, 181
• Mathematical model of caprine arthritis encephalitis considering the seasonal breeding
  Hirata T, Yonahara Y, Asharif F, Miyagi T, Omatsu T, Shiroma Y, Mizutani T, Nagata Y, Tamaki S
  Animal and Veterinary Sciences
  2014年06月, 研究論文（学術雑誌）, 共同, 2, 3, 70, 74
• Identification of novel bovine group A rotavirus G15P[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer.
  Masuda T, Nagai M, Yamasato H, Tsuchiaka S, Okazaki S, Katayama Y, Oba M, Nishiura N, Sassa Y, Omatsu T, Furuya T, Koyama S, Shirai J, Taniguchi K, Fujii Y, Todaka R, Katayama K, Mizutani T.
  Vet Microbiol.
  2014年06月, 研究論文（学術雑誌）, 共同, 171, 1-2, 66, 73
• Detection of enterovirus genome sequence from diarrheal feces of goat.
  Omatsu T, Tsuchiaka S, Hirata T, Shiroma Y, Okazaki S, Katayama Y, Oba M, Nishiura N, Sassa Y, Furuya T, Nagai M, Ochiai H, Tamaki S, Mizutani T.
  Virus Genes.
  2014年06月, 研究論文（学術雑誌）, 共同, 48, 3, 550, 552
• Development of farmer support system on dairy and meat industry of goat utilizing ICT
  Hirata T, Miyagi T, Nagata Y, Tamaki S, Omatsu T, Mizutani T
  Agriculture, Forestry and Fisheries
  2014年04月, 研究論文（学術雑誌）, 共同, 3, 2, 121, 127
• Demonstration of marmosets (Callithrix jacchus) as a non-human primate model for secondary dengue virus infection: high levels of viraemia and serotype cross-reactive antibody responses consistent with secondary infection of humans.
  Moi ML, Takasaki T, Omatsu T, Nakamura S, Katakai Y, Ami Y, Suzaki Y, Saijo M, Akari H, Kurane I.
  J Gen Virol.
  2014年03月, 研究論文（学術雑誌）, 共同, 95, 3, 591, 600
• The first identification and retrospective study of Severe Fever with Thrombocytopenia Syndrome in Japan.
  Takahashi T, Maeda K, Suzuki T, Ishido A, Shigeoka T, Tominaga T, Kamei T, Honda M, Ninomiya D, Sakai T, Senba T, Kaneyuki S, Sakaguchi S, Satoh A, Hosokawa T, Kawabe Y, Kurihara S, Izumikawa K, Kohno S, Azuma T, Suemori K, Yasukawa M, Mizutani T, Omatsu T, Katayama Y, Miyahara M, Ijuin M, Doi K, Okuda M, Umeki K, Saito T, Fukushima K, Nakajima K, Yoshikawa T, Tani H, Fukushi S, Fukuma A, Ogata M, Shimojima M, Nakajima N, Nagata N, Katano H, Fukumoto H, Sato Y, Hasegawa H, Yamagishi T, Oishi K, Kurane I, Morikawa S, Saijo M.
  J Infect Dis.
  2014年03月, 研究論文（学術雑誌）, 共同, 209, 6, 816, 827
• Existence of feline morbillivirus infection in Japanese cat populations.
  Furuya T, Sassa Y, Omatsu T, Nagai M, Fukushima R, Shibutani M, Yamaguchi T, Uematsu Y, Shirota K, Mizutani T.
  Arch Virol.
  2014年02月, 研究論文（学術雑誌）, 共同, 159, 2, 371, 373
• Detection of bovine group a rotavirus using rapid antigen detection kits, rt-PCR and next-generation DNA sequencing.
  Minami-Fukuda F, Nagai M, Takai H, Murakami T, Ozawa T, Tsuchiaka S, Okazaki S, Katayama Y, Oba M, Nishiura N, Sassa Y, Omatsu T, Furuya T, Koyama S, Shirai J, Tsunemitsu H, Fujii Y, Katayama K, Mizutani T.
  J Vet Med Sci.
  2013年12月, 研究論文（学術雑誌）, 共同, 75, 12, 1651, 1655
• A Study on Mathematical Model of Caprine Arthritis Encephalitis (CAE) and Development of Animal Quarantine Information System Adapted for Small Island
  Hirata T, Yonahara Y, Asharif F, Omatsu T, Miyagi T, Nagata Y, Mizutani T, Tamaki S
  IEEK Transactions on Smart Processing and Computing
  2013年08月, 研究論文（学術雑誌）, 共同, 2, 4, 302, 308
• Molecular epidemiology of avian bornavirus from pet birds in Japan.
  Sassa Y, Horie M, Fujino K, Nishiura N, Okazaki S, Furuya T, Nagai M, Omatsu T, Kojima A, Mizugami M, Ueda K, Iki H, Ebisawa K, Tomonaga K, Mizutani T.
  Virus Genes.
  2013年08月, 研究論文（学術雑誌）, 共同, 47, 1, 173, 7
• Dynamics of cellular immune responses in the acute phase of dengue virus infection.
  Yoshida T, Omatsu T, Saito A, Katakai Y, Iwasaki Y, Kurosawa T, Hamano M, Higashino A, Nakamura S, Takasaki T, Yasutomi Y, Kurane I, Akari H.
  Arch Virol.
  2013年06月, 研究論文（学術雑誌）, 共同, 158, 6, 1209, 20
• Detection of dengue virus nonstructural protein 1 (NS1) by using ELISA as a useful laboratory diagnostic method for dengue virus infection of international travelers.
  Moi ML, Omatsu T, Tajima S, Lim CK, Kotaki A, Ikeda M, Harada F, Ito M, Saijo M, Kurane I, Takasaki T.
  J Travel Med.
  2013年05月, 研究論文（学術雑誌）, 共同, 20, 3, 185, 93
• Changes in hematological and serum biochemical parameters in common marmosets (Callithrix jacchus) after inoculation with dengue virus.
  Omatsu T, Moi ML, Takasaki T, Nakamura S, Katakai Y, Tajima S, Ito M, Yoshida T, Saito A, Akari H, Kurane I.
  J Med Primatol.
  2012年10月, 研究論文（学術雑誌）, 共同, 41, 5, 289, 96
• Detection of dengue virus genome in urine by real-time reverse transcriptase PCR: a laboratory diagnostic method useful after disappearance of the genome in serum.
  Hirayama T, Mizuno Y, Takeshita N, Kotaki A, Tajima S, Omatsu T, Sano K, Kurane I, Takasaki T.
  J Clin Microbiol.
  2012年06月, 研究論文（学術雑誌）, 共同, 50, 6, 2047, 52
• CD16(+) natural killer cells play a limited role against primary dengue virus infection in tamarins.
  Yoshida T, Omatsu T, Saito A, Katakai Y, Iwasaki Y, Iijima S, Kurosawa T, Hamano M, Nakamura S, Takasaki T, Yasutomi Y, Kurane I, Akari H.
  Arch Virol.
  2012年02月, 研究論文（学術雑誌）, 共同, 157, 2, 363, 8

研究発表、招待講演等

• フィリピンに生息する野生のコウモリを対象としたCoronavirusおよびPteropine Orthoreovirusの疫学的調査
  第63回獣医疫学会学術集会
  2024年03月17日, 口頭発表（一般）
• コウモリ由来コロナウイルス感染症の流行予測に向けた疫学調査
  第166回日本獣医学会学術集会
  2023年09月05日, 口頭発表（一般）
• 耐酸性微細藻類バイオ医薬品プラットフォームとしての社会実装の試み
  JST未来社会創造事業　本格研究キックオフシンポジウム
  2023年08月23日, シンポジウム・ワークショップ パネル（指名）
• 野生動物を対象とした耐性菌保有状況と地理特性との相関解析
  第165回日本獣医学会学術集会
  2022年09月06日, 口頭発表（一般）
• コウモリの小腸オルガノイド培養法の確立
  第163回日本獣医学会学術集会
  2020年09月14日, 口頭発表（一般）
• 衛生意識向上の動機づけとしての食鳥検査記録の利用の検討
  第56回獣医疫学会学術集会
  2020年09月05日, 口頭発表（一般）
• 新型コロナウイルスの自然宿主とされるコウモリについて
  第56回獣医疫学会学術集会
  2020年09月05日, シンポジウム・ワークショップ パネル（指名）
• 西アフリカの大型草食齧歯類グラスカッターと反芻家畜の腸内菌叢比較
  第161回日本獣医学会学術集会
  2019年09月12日, 口頭発表（一般）
• 牛ウイルス性下痢ウイルスのEND（－）は宿主の自然免疫誘導下でEND（+）の増殖を支持する
  第161回日本獣医学会学術集会
  2018年09月13日, 口頭発表（一般）
• Development of Detection System for Small Ruminant Lentivirus in Japan
  International Scientific Symposium "Enhancement of interactive communication and collaborative activities between researchers through sharing the current research in One Health and infectious disease"
  2017年11月24日, シンポジウム・ワークショップ パネル（公募）
• Epidemiology for Risk Assessment of Zoonoses from Bats
  Endemic Species Research Institute Workshop
  2017年10月16日, シンポジウム・ワークショップ パネル（指名）
• Epidemiology of small ruminant lentivirus in Japan
  International Young Scientists’ Symposium
  2016年11月29日, その他
• 日本国内農場における山羊関節炎・脳脊髄炎（CAEV）の浸潤状況について
  第18回全国山羊サミット
  2016年11月12日, その他
• 日本国内農場における山羊関節炎・脳脊髄炎ウイルス（CAEV）の浸潤状況について
  第159回日本獣医学会学術集会
  2016年09月08日, その他
• Development and validation of a novel system for detection and differentiation of bovine abortive pathogens by real-time PCR
  第159回日本獣医学会学術集会
  2016年09月07日, その他
• TaqManリアルタイムPCR法による牛呼吸器複合病関連病原体の網羅的検査系の開発
  第159回日本獣医学会学術集会
  2016年09月07日, その他
• 日本の牛エンテロウイルスの分子系統解析
  第159回日本獣医学会学術集会
  2016年09月07日, その他
• 野生水鳥由来のH5N2亜型低病原性鳥インフルエンザウイルス（AIV）のマウス継代感染による遺伝子変異と病原性の変化
  第159回日本獣医学会学術集会
  2016年09月07日, その他
• ネコパラミクソウイルスの国内初の検出
  第159回日本獣医学会学術集会
  2016年09月07日, その他
• 一本鎖DNAウイルスゲノムの迅速検出法の開発
  第159回日本獣医学会学術集会
  2016年09月07日, その他
• 日本の河川、沿岸域およびマリアナ海域におけるニホンウナギのJEECウイルス感染率
  第159回日本獣医学会学術集会
  2016年09月07日, その他
• ペットのフトアゴトカゲに感染しているアガマアデノウイルスの検出
  第159回日本獣医学会学術集会
  2016年09月07日, その他
• ヨツユビハリネズミ（Atelerix albiventris）の気管炎からのスカンクアデノウイルス（SkAdV-1）の検出
  第159回日本獣医学会学術集会
  2016年09月07日, その他
• ヤギの疾病および未知ウイルスの検出について
  平成27年度日本獣医師会獣医学術学会年次大会
  2016年02月, 口頭発表（招待・特別）
• 牛糞便サンプルの次世代シークエンスによるウイルス遺伝子解析
  平成27年度　関東・東京合同地区　獣医師大会・三学会
  2015年09月, その他
• フィリピンのコウモリから分離されたプテロパインオルソレオウイルスの性状解析及び疫学 的研究
  第158回日本獣医学会
  2015年09月, その他
• 次世代シークエンスを利用した牛下痢便サンプルのウイルス遺伝子全長解析による牛感染性下痢症の原因解析
  第82回家畜衛生学会
  2015年07月, その他
• デングウイルス感染症
  第82回家畜衛生学会
  2015年07月, 口頭発表（招待・特別）
• How to prevent and control rabies? –From the viewpoint of socio-epidemiology
  Tokyo-Chiang Mai World Rabies Day special conference
  2014年10月14日, 口頭発表（招待・特別）
• 山羊関節炎・脳脊髄炎ウイルスの国内浸潤状況について
  第157回日本獣医学会学術集会
  2014年09月12日, その他
• TaqManリアルタイムPCRを用いたウシ下痢関連病原体の網羅的診断系の開発
  第157回日本獣医学会学術集会
  2014年09月11日, その他
• フィリピンの野生コウモリから検出された新規ヘルペスウイルス遺伝子
  第157回日本獣医学会学術集会
  2014年09月11日, その他
• 動物園のライオンから分離されたレオウイルスの遺伝学的特徴
  第157回日本獣医学会学術集会
  2014年09月10日, その他
• 次世代シークエンサーを利用してのウエルシュ菌新型下痢毒素遺伝子の同定とゲノム解析の試み
  第157回日本獣医学会学術集会
  2014年09月09日, その他
• 次世代シークエンサーを用いた2本鎖RNAウイルス検出法の確立
  第157回日本獣医学会学術集会
  2014年09月09日, その他
• WOBBLY HEDGEHOG SYNDROMEが疑われたヨツユビハリネズミの脳炎からのマウス肺炎ウイルスの検出
  第157回日本獣医学会学術集会
  2014年09月09日, その他
• リアルタイムPCR検査による日本のネコモルビリウイルス陽性率と株間の比較
  第157回日本獣医学会学術集会
  2014年09月09日, その他
• 日本で分離されたG3およびG14型ウマロタウイルスの全ゲノム解析
  第157回日本獣医学会学術集会
  2014年09月09日, その他
• スーパーショートRNA泳動パターンが認められたH2 NSP5遺伝子型のブタロタウイルスの検出
  第157回日本獣医学会学術集会
  2014年09月09日, その他
• フィリピンのコウモリからのネルソンベイグループに分類されるオルソレオウイルスの分離
  第157回日本獣医学会学術集会
  2014年09月09日, その他
• アカバネウイルスによるアポトーシスメカニズムの解明
  第157回日本獣医学会学術集会
  2014年09月09日, その他
• A Study on Crisis Management of Animal Infectious Disease:Mathematical Model of Infectious Diseases of Goat Including Seasonal Variation of Reprodcution
  The International Conference on Applied and Theoretical Information Systems Research, 2013
  2013年11月22日, その他
• A Study of Mathematical Model of Infection Spread on Caprine Arthritis Encephalitis and Its Application
  The 45th ISCIE International Symposium on Stochastic Systems Theory and Its Applications
  2013年11月02日, その他
• ヤギの下痢便から検出された新規エンテロウイルスの次世代シークエンサーを用いたメタゲノム解析
  第156回日本獣医学会学術集会
  2013年09月21日, その他
• 動物園飼育大型ネコ科動物に発生した感染症における病原微生物の特定
  第156回日本獣医学会学術集会
  2013年09月21日, その他
• 糞便を材料とした次世代シークエンスによるウシA群ロタウイルスの遺伝子型
  第156回日本獣医学会学術集会
  2013年09月21日, その他
• 日本におけるネコモルビリウイルスの検出
  第156回日本獣医学会学術集会
  2013年09月21日, その他
• 野生イノシシから分離された新規ラブドウイルスの系統解析
  第156回日本獣医学会学術集会
  2013年09月21日, その他
• 北海道の野鳥におけるボルナウイルスの浸潤状況
  第156回日本獣医学会学術集会
  2013年09月21日, その他
• フィリピンのコウモリからのSFTSウイルスと交差する抗体の検出
  第156回日本獣医学会学術集会
  2013年09月20日, その他
• Design of Information Management System for Livestock Quarantine Adapted to Small Islands
  The 28th International Technical Conference on Circuits/Systems, Computers and Communications
  2013年06月01日, その他

外部研究資金等

• 遺伝子組み換えシゾンの防疫飼料としての利用
  受託研究, 自 2017年02月01日, 至 2017年03月31日

委員歴

• 獣医学教育支援機構
  CBT委員
  自 20240401, 至 20250331
• 獣医疫学会
  幹事（組織強化担当）
  自 20230401, 至 20240331
• 獣医疫学会
  幹事
  自 20180401, 至 20210331
• 獣医疫学会
  幹事
  自 20160401, 至 20180331
• 獣医疫学会
  幹事
  自 20140401, 至 20160331

メディア報道

• コウモリ　空飛ぶ”ウイルス貯蔵庫”
  静岡新聞
  自 2023年11月05日, 至 2023年11月05日
• 空飛ぶウイルス貯蔵庫
  埼玉新聞
  自 2023年11月01日, 至 2023年11月01日
• コウモリの免疫　研究進む
  中国新聞
  自 2023年11月01日, 至 2023年11月01日
• 空飛ぶウイルス貯蔵庫
  山陰中央新聞
  自 2023年10月30日, 至 2023年10月30日
• コウモリはウイルスの貯蔵庫
  大分合同新聞
  自 2023年10月30日, 至 2023年10月30日
• 空飛ぶウイルス貯蔵庫
  中部経済新聞
  自 2023年10月28日, 至 2023年10月28日
• ウイルス”貯蔵庫”　免疫注目
  東京新聞
  自 2023年10月26日, 至 2023年10月26日
• ウイルス"貯蔵庫" 免疫注目コロナ宿主コウモリ 感染症予防などに光
  ウイルス貯蔵庫ともたとえられるコウモリが、人の病気予防に生かせる可能性があるして、研究者の注目を集めている。コウモリの研究を行う大松先生の研究、コメントが紹介される。
  東京新聞
  自 2023年10月26日, 至 2023年10月26日
• コウモリはウイルスの貯蔵庫
  高知新聞
  自 2023年10月22日, 至 2023年10月22日
• コウモリはウイルス貯蔵庫新型コロナで世界揺るがす独特な免疫 研究者注目
  ウイルス貯蔵庫ともたとえられるコウモリが、人の病気予防に生かせる可能性があるして、研究者の注目を集めている。コウモリの研究を行う大松先生の研究、コメントが紹介される。
  高知新聞
  自 2023年10月22日, 至 2023年10月22日
• Why bats? The common secret behind COVID and Ebola
  デマレルーセットオオコウモリの性質について東京農工大学の大松勉准教授のコメントが紹介される。
  Nikkei Asia
  自 2021年12月31日, 至 2021年12月31日
• Intestine 'organoid' grown in lab to study why bats live with viruses but don't get sick
  デマレルーセットオオコウモリの性質について東京農工大学の大松勉准教授のコメントが紹介される。
  Medical Express／News Medical／Lab Manager／Tech Explorist
  自 2021年12月29日, 至 2021年12月31日
• コウモリはなぜウイルスの貯蔵庫　コロナで科学者注目
  デマレルーセットオオコウモリの性質について東京農工大学の大松勉准教授のコメントが紹介される。
  日本経済新聞／WEB
  自 2021年12月25日, 至 2021年12月26日
• コウモリの腸を再現
  東京農工大学の大松勉准教授らが、ルーセットオオコウモリの腸を再現したミニ臓器(ミニ腸)の作製に成功し、それを使用した研究の結果、オオコウモリの仲間が新型コロナウイルス媒介に関わっていない可能性が高いことが紹介される。
  日本経済新聞／WEB
  自 2021年12月05日, 至 2021年12月05日
• 新型コロナ　起源なお謎
  東京農工大学の大松勉准教授らが、フィリピンのチームと共同で現地のコウモリの分布・移動範囲・気象情報などを集め、ウイルスの広がりを予測するシステムの開発をめざしていることが紹介される。
  読売新聞
  自 2021年11月28日, 至 2021年11月28日
• コウモリが新型コロナの感染伝播に関与していない可能性、農工大がミニ臓器で確認
  東京農工大学の大松勉准教授らが、ルーセットオオコウモリの腸を再現したミニ臓器(ミニ腸)の作製に成功し、それを使用した研究の結果、ルーセットオオコウモリが新型コロナウイルス感染症(COVID-19)の感染伝播に関与していないことが示唆されたと発表したことが紹介される。
  マイナビニュース／gooニュース／ニコニコニュース／BIGLOBE／exciteニュース／マピオンニュース
  自 2021年11月18日, 至 2021年11月18日
• 感染症防げ　コウモリ研究
  東京農工大の大松勉准教授（人獣共通感染症学）の研究チームの取り組みが紹介される。
  産経新聞
  自 2021年04月11日, 至 2021年04月11日
• コロナ　-共闘と共生-
  東京農工大学の大松勉准教授（人獣共通感染症学）と水谷哲也教授（ウイルス学）のコメントが紹介される。
  産経新聞
  自 2020年06月04日, 至 2020年06月04日
• 解剖　先端拠点
  東京農工大学 国際家畜感染症防疫研究教育センター「不明」残さぬ検出法探る
  日経産業新聞
  自 2018年01月24日, 至 2018年01月24日

所属学協会

• 獣医疫学会
  自 2014年04月
• 日本獣医学会