研究者データベース
佐々木 一昭SASAKI Kazuakiササキ カズアキ
| ■所属部署名 | 農学研究院 動物生命科学部門 | |||
| ■職名 | 准教授 | |||
Last Updated :2023/03/24
業績情報
氏名・連絡先
氏名
ササキ カズアキ, 佐々木 一昭, SASAKI Kazuaki
主たる所属・職名
農学研究院 動物生命科学部門, 准教授
その他の所属
経歴
- -
（株）日立製作所基礎研究所
自 1992年04月01日, 至 1997年03月31日 - -
（株）日立製作所中央研究所研究員
自 1997年04月01日, 至 2004年09月30日 - -
（株）日立製作所 中央研究所 主任研究員
自 2004年10月01日, 至 2007年03月31日 - -
東京農工大学獣医薬理学研究室准教授
自 2007年04月01日
学歴
学位
免許・資格
研究テーマ
担当授業科目
- 基盤薬理学
2020年 - 臨床薬理学
2020年 - 薬理学実習
2020年 - 基盤薬理学
2019年 - 臨床薬理学
2019年 - 薬理学実習
2019年 - 基盤薬理学
2018年 - 臨床薬理学
2018年 - 薬理学実習
2018年 - 基盤薬理学
2017年 - 臨床薬理学
2017年 - 薬理学実習
2017年 - 統合薬理学
2017年 - 遺伝学
2017年 - 薬理学実習
2016年 - 統合薬理学
2016年 - 基盤薬理学
2016年 - 臨床薬理学
2016年 - 遺伝学
2016年 - 基盤薬理学
2015年 - 薬理学実習
2015年 - 遺伝学
2015年 - 融合科目４（人の生活における獣医学の役割）
2015年 - 遺伝学
2015年 - 農学基礎ゼミ
2015年 - 獣医臨床薬理学
2014年 - 融合科目（人の生活における獣医学の役割）
2014年 - 基盤薬理学
2014年 - 遺伝学
2014年 - 薬理学実習
2014年 - 獣医薬理学Ⅱ
2013年 - 獣医薬理学Ⅰ
2013年 - 融合科目5（人の生活における獣医学の役割）
2013年 - 獣医臨床薬理学
2013年 - 獣医薬理学実習
2013年 - 病態獣医学特論IIa
2012年 - 病態獣医学特別演習I
2012年 - 病態獣医学特論IIb
2012年 - 病態獣医学特論Ib
2012年 - 病態獣医学特別演習II
2012年 - 獣医薬理学Ⅱ
2012年 - 融合科目5（人の生活における獣医学の役割）
2012年 - 獣医薬理学実習
2012年 - 獣医臨床薬理学
2012年 - 獣医薬理学Ⅰ
2012年 - 放射線生物学
2012年 - 融合科目（人と獣医学の関わり）
2011年 - 融合科目（人と獣医学の関わり）
2011年 - 特別研究（基礎・応用）
2011年 - 農学基礎ゼミ
2011年 - 獣医臨床実習
2011年 - 獣医学概論
2011年 - 情報処理学
2011年 - 獣医臨床薬理学
2011年 - 獣医学演習（基礎・応用）
2011年 - 獣医学演習（臨床）
2011年 - 特別研究（基礎・応用）
2011年 - 特別研究（臨床）
2011年 - 特別研究（臨床）
2011年 - 病態獣医学特別演習I
2011年 - 病態獣医学特論IIa
2011年 - 病態獣医学特別演習II
2011年 - 病態獣医学特論Ib
2011年 - 病態獣医学特論IIb
2011年 - 特別研究
2010年 - 獣医薬理学実習
2010年 - 特別実験Ⅱ（基礎）
2010年 - 特別演習Ⅱ（基礎）
2010年 - 獣医臨床薬理学
2010年 - 獣医薬理学Ⅱ
2010年 - 特別研究（基礎・応用）
2010年 - 獣医薬理学Ⅰ
2010年 - 獣医学演習（基礎・応用）
2010年 - 獣医臨床薬理学
2009年 - 獣医薬理学Ⅰ
2009年 - 獣医薬理学Ⅱ
2009年 - 獣医薬理学実習
2009年 - 家畜臨床薬理学
2008年 - 基礎ゼミ
2008年 - 獣医薬理学Ⅰ
2008年 - 獣医薬理学Ⅱ
2008年 - 獣医薬理学実習
2008年 - 家畜薬理学Ⅰ
2007年 - 家畜臨床薬理学
2007年 - 家畜薬理学実験
2007年 - 獣医薬理学Ⅱ
2007年 - 家畜薬理学Ⅱ
2007年 - 獣医薬理学Ⅰ
2007年 - 獣医薬理学実習
2007年
科学研究費助成事業
- 基盤研究(B)
三次元培養法と薬物動態解析の融合による猫乳腺がん制御機構の解明
自 2021年, 至 2024年 - 基盤研究(C)
音響キャビテーション誘導による空間選択的抗癌剤取り込み技術の研究
自 2016年, 至 2018年
論文
- 健常犬におけるテルミサルタンの血中動態解析と薬用量の決定
動物の循環器
2021年03月28日, 研究論文（学術雑誌）, 54, 2, 61‒68 - The utility of electrocardiography and echocardiography in copper deficiency-induced cardiac damage in goats
Mandour, Ahmed S.; Elsayed, Roushdy F.; Ali, Asmaa O.; Mahmoud, Ahmed E.; Samir, Haney; Dessouki, Amina A.; Matsuura, Katsuhiro; Watanabe, Izumi; Sasaki, Kazuaki; Al-Rejaie, Salim; Yoshida, Tomohiko; Shimada, Kazumi; Tanaka, Ryou; Watanabe, Gen
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
SPRINGER HEIDELBERG
Copper deficiency (CuD) is a common mineral disorder in ruminants, which causes histomorphological changes in the heart due to disturbances in copper-dependent metalloenzymes. However, alterations in the measurable cardiac parameters during CuD have not been studied in ruminants, especially in goats. Therefore, the current study aimed to investigate longitudinally the potential role of electrocardiography (ECG) and echocardiography to detect the CuD-induced cardiac damage at different time intervals and concomitantly highlighting the impact of CuD on specific hemato-biochemical parameters and histological cardiac disruption in goats. Eight Shiba goats were included and divided into two equal groups; copper adequate (CuA) as a control and copper-deficient (CuD) that supplemented with copper-chelating agents (sulfur 3 g/kg DM and molybdenum 40 mg/kg DM). The hemato-biochemical analysis, ECG assessment at the base apex lead, and right-side echocardiography were performed just before the experimental onset (T0), and later on at two-time intervals after existing of CuD, at the fifth (T5) and seventh (T7) months. Necropsy and histopathological examination of the heart were performed at the end of the experiment. In the CuD group, the heart dimensions at T5 and T7 showed significant increase in QRS duration, ST-segment duration, the left atrial area in systole, left ventricular diameter and volume in diastole, stroke volume, and cardiac output compared with CuA (P< 0.05). Also, myocardial degeneration, necrosis, and fibrosis were evidenced with a concurrent increase of plasma creatine kinase, lactate dehydrogenase, aspartate aminotransferase, and cardiac troponin I (P< 0.05). In conclusion, CuD disturbs hemato-biochemical parameters and results in myocardial damage and cardiac dilatation that increases some ECG and echocardiographic parameters without development of systolic dysfunction. The ECG and echocardiography can potentially detect cardiac changes in long-lasting CuD in goats.
2021年02月, 研究論文（学術雑誌）, 共同, 28, 7, 0944-1344, DOI(公開)(r-map), 7815, 7827 - Anti-cancer activity of amorphous curcumin preparation in patient-derived colorectal cancer organoids
Elbadawy, Mohamed; Hayashi, Kimika; Ayame, Hiromi; Ishihara, Yusuke; Abugomaa, Amira; Shibutani, Makoto; Hayashi, Shim-Mo; Hazama, Shoichi; Takenouchi, Hiroko; Nakajima, Masao; Tsunedomi, Ryouichi; Suzuki, Nobuaki; Nagano, Hiroaki; Shinohara, Yuta; Kaneda, Masahiro; Yamawaki, Hideyuki; Usui, Tatsuya; Sasaki, Kazuaki
BIOMEDICINE & PHARMACOTHERAPY
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Despite its adverse effects, chemotherapy is generally used for the treatment of colorectal cancer (CRC). Development of supplement preparations targeting cancer stem cells (CSCs) that cause distant metastasis and drug resistance is required. Although curcumin is known to have anti-tumor, hepatoprotective, and hypoglycemic-like actions, its low water solubility, oral absorption, and bioavailability impede its therapeutic uses. Patient-derived organoid cultures can recapitulate heterogeneity, epithelial structures, and molecular imprints of their parental tissues. In the present study, anti-carcinogenic properties of amorphous curcumin (AC), a compound with improved solubility and bioavailability, were evaluated against human CRC organoids. Treatment with AC inhibited the cell viability of CRC organoids in a concentration-dependent manner. AC arrested the cell cycle of CRC organoids and induced apoptosis. AC inhibited phosphorylation of ERK. Expression of downstream signals of ERK, namely c-MYC and cyclin-D1, were inhibited. Expressions of CSC markers, CD44, LGR5, and CD133, were declined in the AC-treated CRC organoids. The combinational treatment of CRC organoids with AC and anti-cancer drugs, oxaliplatin, 5-FU, or irinotecan showed a synergistic activity. In vivo, AC decreased the tumor growth of CRC organoids in mice with the induction of necrotic lesions. In conclusion, AC diminished the cell viability of CRC organoids through the inhibition of proliferation-related signals and CSC marker expression in addition to arresting the cell cycle. Collectively, these data suggest the value of AC as a promising supplement that could be used in combination with anti-cancer drugs to prevent the recurrence and metastasis of CRC.
2021年10月, 研究論文（学術雑誌）, 共同, 142, 0753-3322, DOI(公開)(r-map) - Anti-tumor effect of trametinib in bladder cancer organoid and the underlying mechanism
Elbadawy, Mohamed; Sato, Yomogi; Mori, Takashi; Goto, Yuta; Hayashi, Kimika; Yamanaka, Megumi; Azakami, Daigo; Uchide, Tsuyoshi; Fukushima, Ryuji; Yoshida, Toshinori; Shibutani, Makoto; Kobayashi, Mio; Shinohara, Yuta; Abugomaa, Amira; Kaneda, Masahiro; Yamawaki, Hideyuki; Usui, Tatsuya; Sasaki, Kazuaki
CANCER BIOLOGY & THERAPY
TAYLOR & FRANCIS INC
Bladder cancer (BC), a main neoplasm of urinary tract, is usually inoperable and unresponsive to chemotherapy. As a novel experimental model for muscle-invasive BC, we previously established a culture method of dog BC organoids. In the present study, the detailed in vitro and in vivo anti-tumor effects of trametinib were investigated by using this model. In each BC organoid strain, epidermal growth factor receptor (EGFR)/ERK signaling was upregulated compared with normal bladder cells. Trametinib even at a low concentration inhibited the cell viability of BC organoids and the activation of ERK through decreasing expression of c-Myc, ELK1, SIK1, and PLA2G4A. Trametinib arrested cell cycle of BC with few apoptosis. Dual treatment of BC organoids with trametinib and YAP inhibitor, verteporfin extremely inhibited the cell viability with apoptosis induction. Moreover, trametinib induced basal to luminal differentiation of BC organoids by upregulating luminal markers and downregulating basal ones. In vivo, trametinib decreased the tumor growth of BC organoids in mice and the xenograft-derived organoids from trametinib-administered mice showed enhanced sensitivity to carboplatin due to MSH2 upregulation. Our data suggested a new strategy of trametinib-YAP inhibitor or trametinib-carboplatin combination as a promising treatment of BC.
2021年06月03日, 研究論文（学術雑誌）, 共同, 22, 5-6, 1538-4047, DOI(公開)(r-map), 357, 371 - Effects of dapagliflozin in combination with insulin on cytochrome P450 activities in a diabetes type 1 rat model
Sayed, Noha; Murata, Ikue; Abdalla, Osama; Kilany, Omnia; Dessouki, Amina; Sasaki, Kazuaki
JOURNAL OF VETERINARY MEDICAL SCIENCE
JAPAN SOC VET SCI
Previous studies reported that diabetes alters the activities of hepatic cytochrome P450 (CYP) enzymes, which, in turn, affects the disposition of some drugs. We herein examined and compared the effects of the combination of dapagliflozin with a low insulin dose, a full dose of insulin alone, and dapagliflozin alone for 3 and 8 weeks on CYP activities in a diabetes type 1 rat model. We induced type 1 diabetes in rats using a single intraperitoneal injection of 60 mg/ kg streptozotocin (STZ). Daily treatment with the full dose of insulin alone, dapagliflozin alone, or dapagliflozin in combination with a low dose of insulin was then initiated. STZ-induced rats developed marked hyperglycemia and altered CYP2E activities. Dapagliflozin in combination with a low dose of insulin stabilized hyperglycemia and CYP1A, 2D, 2E and 3A activities. However, dapagliflozin alone did not improve blood glucose levels or CYP activities. These results suggest that the effects of dapagliflozin in combination with a low dose of insulin are similar to those of a full dose of insulin, and stabilize CYP activities in type 1 diabetes.
2021年10月, 研究論文（学術雑誌）, 共同, 83, 10, 0916-7250, DOI(公開)(r-map), 1597, 1603 - Evaluation of the Safety and Feasibility of Apheresis in Dogs: For Application in Metastatic Cancer Research
Yamamoto, Haru; Elbadawy, Mohamed; Fujisaka, Koudai; Sato, Yomogi; Ohmori, Takahiro; Shinohara, Yuta; Hatano, Yui; Kobayashi, Daichi; Gomyo, Ayana; Sudo, Yuji; Azakami, Daigo; Uchide, Tsuyoshi; Fukushima, Ryuji; Morita, Shohei; Abugomaa, Amira; Yamawaki, Hideyuki; Kaneda, Masahiro; Usui, Tatsuya; Sasaki, Kazuaki
ANIMALS
MDPI
In cases of cancer metastasis, some tumor cells circulate in blood (CTCs) and function as a seed for metastasis. These cells are important for detailed study of the molecular basis of metastasis. However, their few numbers and difficult isolation hinder its analysis. Apheresis, a process used to isolate a specific component of blood, can efficiently separate peripheral blood monocyte (PBMC), whose density is closely like that of CTCs. In the present study we used the dogs as a precious model of human's cancers to check the safety and feasibility of apheresis and to separate PBMC and infused MCF7 cells using Spectra Optia apheresis machine (Terumo). The process was performed safely to capture PBMC and MCF7. The captured MCF7 cells were regrown up in vitro and characterized. In conclusion, Spectra Optia apheresis machine (Terumo) can be used safely to isolate CTCs from dogs blood with precautions to keep hemodynamic stability. In patients with solid tumors, circulating tumor cells (CTCs) spread in their blood and function as a seed for metastases. However, the study of CTCs has been limited by their rarity, low frequency, and heterogeneity. The efficient collection of CTCs will contribute to further research of metastatic cancers. Apheresis is a process in which the whole blood of an individual is passed through a machine that isolates a particular constituent and returns the remainder to the circulation. In the present study, we investigated the safety and feasibility of apheresis to separate peripheral blood monocytes (PBMCs), whose density is closely similar to that of CTCs, and to capture intravenously administered human breast cancer cells, MCF7s, from the dogs. No life-threatening events were observed in dogs during the apheresis process. The changes in the hemogram were transient and recovered gradually within a few days after apheresis. During apheresis, 50 mL of PBMCs could be collected from each dog. Notably, a thrombus was formed along the circuit wall during apheresis, which decreased the blood collection pressure. MCF7 cells were successfully captured by the apheresis machine. The captured cells were regrown in vitro and characterized compared with the original cells. In conclusion, apheresis could be safely performed in dogs to isolate CTCs with precautions to maintain hemodynamic stability.
2021年10月, 研究論文（学術雑誌）, 共同, 11, 10, 2076-2615, DOI(公開)(r-map) - Establishment of Intestinal Organoid from Rousettus leschenaultii and the Susceptibility to Bat-Associated Viruses, SARS-CoV-2 and Pteropine Orthoreovirus
Elbadawy, Mohamed; Kato, Yuki; Saito, Nagisa; Hayashi, Kimika; Abugomaa, Amira; Kobayashi, Mio; Yoshida, Toshinori; Shibutani, Makoto; Kaneda, Masahiro; Yamawaki, Hideyuki; Mizutani, Tetsuya; Lim, Chang-Kweng; Saijo, Masayuki; Sasaki, Kazuaki; Usui, Tatsuya; Omatsu, Tsutomu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
MDPI
Various pathogens, such as Ebola virus, Marburg virus, Nipah virus, Hendra virus, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle East Respiratory Syndrome Coronavirus (MERS-CoV), and SARS-CoV-2, are threatening human health worldwide. The natural hosts of these pathogens are thought to be bats. The rousette bat, a megabat, is thought to be a natural reservoir of filoviruses, including Ebola and Marburg viruses. Additionally, the rousette bat showed a transient infection in the experimental inoculation of SARS-CoV-2. In the current study, we established and characterized intestinal organoids from Leschenault's rousette, Rousettus leschenaultii. The established organoids successfully recapitulated the characteristics of intestinal epithelial structure and morphology, and the appropriate supplements necessary for long-term stable culture were identified. The organoid showed susceptibility to Pteropine orthoreovirus (PRV) but not to SARS-CoV-2 in experimental inoculation. This is the first report of the establishment of an expandable organoid culture system of the rousette bat intestinal organoid and its sensitivity to bat-associated viruses, PRV and SARS-CoV-2. This organoid is a useful tool for the elucidation of tolerance mechanisms of the emerging rousette bat-associated viruses such as Ebola and Marburg virus.
2021年10月, 研究論文（学術雑誌）, 共同, 22, 19, DOI(公開)(r-map) - Assessment of correlations and concentrations of salivary and plasma steroids, testicular morphometry, and semen quality in different climatic conditions in goats
Samir, Haney; Nyametease, Paul; Elbadawy, Mohamed; Fathi, Mohamed; Mandour, Ahmed S.; Radwan, Faten; Nagaoka, Kentaro; Sasaki, Kazuaki; Watanabe, Gen
THERIOGENOLOGY
ELSEVIER SCIENCE INC
The current study aimed to investigate whether steroids (testosterone, estradiol, and cortisol) in the saliva of goats reflects their concentrations in the plasma. Also, it aimed at ascertaining, for the first time, the effect of changes in climatic conditions (spring versus summer) on the aforementioned steroids, testicular volume, testicular echotexture (pixel intensity; PI, and integrated density; ID), and semen quality in goats. Saliva and plasma samples were collected from 7 male Shiba goats weekly in spring and summer for measurement of testosterone (T; ng/ml), estradiol (E2; pg/ml), and cortisol (ng/ml) using radioimmunoassay. The changes in testicular volumes (TV/ml) and echogenicity were monitored weekly using ultrasonographic assessments concomitantly with subjective evaluations of semen parameters. Results revealed a highly significant positive correlation (r = 0.72; P < 0.0001) between plasma and salivary cortisol. Mild significant positive correlation was found between salivary and plasma T levels (r = 0.31; P < 0.002). However, low non-significant negative correlation was reported between salivary and plasma E2 levels (r = -0.24; P > 0.05). Higher levels of salivary cortisol were found in summer than in the spring (3.8 +/- 0.6 ng/ml versus 1.4 +/- 0.3 ng/ml; P < 0.001). However, no differences in levels of T, E2, and plasma cortisol were recorded between spring and summer (P > 0.05). Values of testicular PI and ID were significantly (P < 0.001) higher in the summer (94.5 +/- 4.9, 258,388 +/- 13,190, respectively) than in the spring (74.0 +/- 2.1, 200,922 +/- 5704, respectively). Meanwhile, TV and semen parameters did not significantly differ between spring and summer. In conclusion, saliva can be considered as an alternative biological fluid for the measurement of cortisol and T, but not suitable for E2. Climatic conditions significantly impact the levels of cortisol in saliva and testicular echogenicity in goats. (C) 2020 Elsevier Inc. All rights reserved.
2020年11月, 研究論文（学術雑誌）, 共同, 157, 0093-691X, DOI(公開)(r-map), 238, 244 - Establishment of 2.5D organoid culture model using 3D bladder cancer organoid culture
Abugomaa, Amira; Elbadawy, Mohamed; Yamanaka, Megumi; Goto, Yuta; Hayashi, Kimika; Mori, Takashi; Uchide, Tsuyoshi; Azakami, Daigo; Fukushima, Ryuji; Yoshida, Toshinori; Shibutani, Makoto; Yamashita, Risako; Kobayashi, Mio; Yamawaki, Hideyuki; Shinohara, Yuta; Kaneda, Masahiro; Usui, Tatsuya; Sasaki, Kazuaki
SCIENTIFIC REPORTS
NATURE PUBLISHING GROUP
Three-dimensional (3D) organoid culture holds great promises in cancer precision medicine. However, Matrigel and stem cell-stimulating supplements are necessary for culturing 3D organoid cells. It costs a lot of money and consumes more time and effort compared with 2D cultured cells. Therefore, the establishment of cheaper and Matrigel-free organoid culture that can maintain the characteristics of a part of 3D organoids is demanded. In the previous study, we established a dog bladder cancer (BC) 3D organoid culture system by using their urine samples. Here, we successfully isolated cells named 2.5D organoid from multiple strains of dog BC 3D organoids using 2.5 organoid media. The cell proliferation speed of 2.5D organoids was faster than parental 3D organoid cells. The expression pattern of stem cell markers was close to 3D organoids. Injection of 2.5D organoid cells into immunodeficient mice formed tumors and showed the histopathological characteristics of urothelial carcinoma similar to the injection of dog BC 3D organoids. The 2.5D organoids had a similar sensitivity profile for anti-cancer drug treatment to their parental 3D organoids. These data suggest that our established 2.5D organoid culture method might become a reasonable and useful tool instead of 3D organoids in dog BC research and therapy.
2020年06月10日, 研究論文（学術雑誌）, 共同, 10, 1, 2045-2322, DOI(公開)(r-map) - Effects of several organophosphates on hepatic cytochrome P450 activities in rats
Abdou, Rania H.; Elbadawy, Mohamed; Khalil, Waleed F.; Usui, Tatsuya; Sasaki, Kazuaki; Shimoda, Minoru
JOURNAL OF VETERINARY MEDICAL SCIENCE
JAPAN SOC VET SCI
Four commonly used organophosphates (fenitrothion, dichlorvos, chlorpyrifos, and trichlorfon) were orally administered to male Sprague-Dawley rats for five days in order to explore their effects on the activities of liver cytochrome P450 (CYP). In addition, Michaelis-Menten kinetics of the metabolic reactions catalyzed by liver CYPs were analyzed following the addition of these compounds to the assay system to examine their potential inhibitory effects on liver CYPs activities. These reactions included ethoxyresorufin O-deethylation, midazolam 4-hydroxylation, tolbutamide hydroxylation, and bufuralol 1'-hydroxylation for CYP1A, 3A, 2C, and 2D activities, respectively. Total CYP content was also examined after oral administration of each organophosphate. Results revealed that oral giving of fenitrothion inhibited significantly CYP1A and 3A activities while elevated activity of CYP2C. Fenitrothion is a potent inhibitor for CYP1A and 2C with Ki values of 0.42 and 36.1 mu M, respectively but had a weak inhibitory effect on CYP2D and 3A with Ki values of 290 and 226 mu M, respectively. Chlorpyrifos is a potent inhibitor of CYP1A with Ki 0.24 mu M and moderately inhibited CYP2C or 3A with Ki values of 84.8 and 77.7 mu M, respectively. On the other hand, dichlorvos and trichlorfon caused extremely low or negligible inhibition of different CYP activities. From these results, it is concluded that both fenitrothion and chlorpyrifos may increase the toxicity of chemicals in environmental living organisms through their potent inhibitory effects on these CYP activities, but dichlorvos and trichlorfon may not.
2020年05月, 研究論文（学術雑誌）, 共同, 82, 5, 0916-7250, DOI(公開)(r-map), 598, 606 - Development of Prostate Cancer Organoid Culture Models in Basic Medicine and Translational Research
Elbadawy, Mohamed; Abugomaa, Amira; Yamawaki, Hideyuki; Usui, Tatsuya; Sasaki, Kazuaki
CANCERS
MDPI
Prostate cancer (PC) is the most prevalent cancer in men and the second main cause of cancer-related death in Western society. The lack of proper PC models that recapitulate the molecular and genomic landscape of clinical disease has hampered progress toward translational research to understand the disease initiation, progression, and therapeutic responses in each patient. Although several models have been developed, they hardly emulated the complicated PC microenvironment. Precision medicine is an emerging approach predicting appropriate therapies for individual cancer patients by means of various analyses of individual genomic profiling and targeting specific cancer pathways. In PC, precision medicine also has the potential to impose changes in clinical practices. Here, we describe the various PC models with special focus on PC organoids and their values in basic medicine, personalized therapy, and translational researches in vitro and in vivo, which could help to achieve the full transformative power of cancer precision medicine.
2020年04月, 研究論文（学術雑誌）, 共同, 12, 4, DOI(公開)(r-map) - Effect of dapagliflozin alone and in combination with insulin in a rat model of type 1 diabetes
Sayed, Noha; Abdalla, Osama; Kilany, Omnia; Dessouki, Amina; Yoshida, Toshinori; Sasaki, Kazuaki; Shimoda, Minoru
JOURNAL OF VETERINARY MEDICAL SCIENCE
JAPAN SOC VET SCI
Dapagliflozin is a selective sodium-glucose cotransporter 2 (SGLT2) inhibitor; it reduces glucose reabsorption via the kidney and increases the glucose excretion in urine. This inhibitor functions through a unique insulin-independent mechanism, and is therefore a potential new approach for the treatment of hyperglycemia in patients with diabetes. In this study, we evaluated the effectiveness of the SGLT2 inhibitor, dapagliflozin, by using a rat model of type 1 diabetes. Type 1 diabetes was induced by a single intraperitoneal injection of 60 mg/kg streptozotocin (STZ). The STZ-induced rats showed marked hyperglycemia and other metabolic abnormalities. We clarified the hypoglycemic effect of the combination treatment of dapagliflozin with a low dose of insulin compared with dapagliflozin alone and insulin alone in 3-week and 8-week studies. Our results showed that dapagliflozin in combination with a low dose of insulin significantly lowered hyperglycemia, hypercholesterolemia, and hypertriglyceridemia. Furthermore, the antioxidant status and body weight were improved. In contrast, treatment with dapagliflozin alone did not improve the blood glucose levels, lipid profile, antioxidant status, or body weight. These findings suggested that in type 1 diabetes, dapagliflozin was effective in combination with a low dose of insulin; however, the administration of dapagliflozin alone did not achieve a significant effect.
2020年04月, 研究論文（学術雑誌）, 共同, 82, 4, 0916-7250, DOI(公開)(r-map), 467, 474 - Efficacy of primary liver organoid culture from different stages of non-alcoholic steatohepatitis (NASH) mouse model
Elbadawy, Mohamed; Yamanaka, Megumi; Goto, Yuta; Hayashi, Kimika; Tsunedomi, Ryouichi; Hazama, Shoichi; Nagano, Hiroaki; Yoshida, Toshinori; Shibutani, Makoto; Ichikawa, Ryo; Nakahara, Junta; Omatsu, Tsutomu; Mizutani, Tetsuya; Katayama, Yukie; Shinohara, Yuta; Abugomaa, Amira; Kaneda, Masahiro; Yamawaki, Hideyuki; Usui, Tatsuya; Sasaki, Kazuaki
BIOMATERIALS
ELSEVIER SCI LTD
Non-alcoholic steatohepatitis (NASH) is associated with liver fibrosis and cirrhosis, which eventually leads to hepatocellular carcinoma. Although several animal models were developed to understand the mechanisms of NASH pathogenesis and progression, it remains obscure. A 3D organoid culture system can recapitulate organ structures and maintain gene expression profiles of original tissues. We therefore tried to generate liver organoids from different degrees [defined as mild (NASH A), moderate (NASH B) and severe (NASH C)] of methionine- and choline-deficient diet-induced NASH model mice and analyzed the difference of their architecture, cell components, organoid-forming efficacy, and gene expression profiles. Organoids from each stage of NASH model mice were successfully generated. Interestingly, epithelial-mesenchymal transition was observed in NASH C organoids. Expression of Collagen I and an activated hepatic stellite cell marker, alpha-sma was upregulated in the liver organoids from NASH B and C mice. The analysis of RNA sequencing revealed that several novel genes were upregulated in all NASH liver organoids. These results suggest that our generated liver organoids from different stages of NASH diseased mice might become a useful tool for in vitro studies of the molecular mechanism of NASH development and also for identifying novel biomarkers for early diagnosis of NASH disease.
2020年04月, 研究論文（学術雑誌）, 共同, 237, 0142-9612, DOI(公開)(r-map) - Passive immunization against inhibin increases testicular blood flow in male goats
Samir, Haney; El Sayed, Mohamed A. I.; Nagaoka, Kentaro; Sasaki, Kazuaki; Abo El-Maaty, Amal M.; Karen, Aly; Abou-Ahmed, Mostafa M.; Watanabe, Gen
THERIOGENOLOGY
ELSEVIER SCIENCE INC
The present study investigated whether or not passive immunization against inhibin modulates testicular blood flow in goats. Male Shiba goats were injected with either 10 ml of inhibin antiserum (INH group; n = 5) or 10 ml of normal castrated goat serum (NGS group; n = 4). Concentrations of FSH, LH, testosterone (T), and estradiol (E2) in the plasma were measured by radioimmunoassay. Blood flow within the supratesticular (STA) and marginal testicular arteries (MTA) were measured by color pulsed Doppler ultrasonography, and Doppler indices (resistive index; RI and pulsatility index; PI) were recorded. Results revealed significant increases in concentrations of FSH and E2 in the INH group compared to those in the NGS group (P < 0.05). Animals in the INH group had greater (P < 0.05) FSH concentrations than those in the NGS group in the period between 60 h and 144 h after treatment than at any other time. Estradiol concentrations were greater (P < 0.05) in the INH group than in the NGS group at 6 h (12.15 +/- 2.09 pg/ml vs 5.49 +/- 1.17 pg/mL), 12 h (8.27 +/- 1.29 pg/mL vs 3.05 +/- 0.38 pg/mL), and 36 h (935 +/- 1.31 pg/mL vs 5.09 +/- 0.46 pg/mL) after treatment than at any other time. Concentrations of LH and T did not significantly change between the two groups. Goats in the INH group had lesser (P < 0.05) RI of the STA than those in the NGS group and RI values were lesser at 24 h (0.37 +/- 0.031 vs 0.49 +/- 0.004) and 120 h (0.38 +/- 0.028 vs 0.55 +/- 0.048) after treatment than at any other time. Furthermore, values of RI and PI of the MTA were significantly lesser (P < 0.05) in the INH group compared to those in the control group at 48 h (RI of MTA: 0.21 +/- 0.014 vs 0.37 +/- 0.039; PI of MTA: 0.24 +/- 0.016 vs 0.46 +/- 0.058) after treatment than at any other time. In conclusion, passive immunization against inhibin has a stimulatory effect on testicular blood flow in goats by inducing decreases in the RI values of the STA and MTA. (C) 2020 Elsevier Inc. All rights reserved.
2020年04月15日, 研究論文（学術雑誌）, 共同, 147, 0093-691X, DOI(公開)(r-map), 85, 91 - Administration of melatonin improves testicular blood flow, circulating hormones, and semen quality in Shiba goats
Samir, Haney; Nyametease, Paul; Elbadawy, Mohamed; Nagaoka, Kentaro; Sasaki, Kazuaki; Watanabe, Gen
THERIOGENOLOGY
ELSEVIER SCIENCE INC
Despite the role of melatonin in the regulation of the sleep-wake cycle and seasonal-reproduction, the present study investigated, for the first time, the potential role of melatonin on testicular blood flow (TBF) in goats. Twelve sexually mature male Shiba goats were exposed to a single s.c. injection of either melatonin suspended in one ml of corn oil (melatonin group; 36 mg/goat; n = 5) or one ml of corn oil (control group; n = 7). Monitoring the changes in TBF was done one week before (W-1), at the time of injection (W0), and once a week for 8 weeks after injection using color-pulsed Doppler ultrasonography. Concentrations of FSH, LH, inhibin, testosterone (T), estradiol (E2), and insulin-like growth factor-1 (IGF-1) in plasma were determined by radioimmunoassay. Melatonin and nitric oxide (NO) concentrations were measured using enzyme immunoassay kits. Moreover, semen collection and evaluation of some sperm parameters were performed once a week. Results revealed decreases (P < 0.05) in the Doppler indices (resistive index, pulsatility index) of the testicular arteries from W2 till W6 in the melatonin group. FSH, LH, and inhibin concentrations did not change between the two groups, while T, E2, NO, and melatonin concentrations increased (P < 0.05) in the melatonin group compared to the control. Estradiol and NO concentrations increased (P < 0.05), coinciding with decreases in the values of Doppler indices. Notable (P < 0.05) improvements in most parameters of semen quality were seen in the melatonin group. In conclusion, melatonin induced a stimulatory effect on TBF in Shiba goats and possibly, it could be a potential to improve male goats fertility. (C) 2020 Elsevier Inc. All rights reserved.
2020年04月01日, 研究論文（学術雑誌）, 共同, 146, 0093-691X, DOI(公開)(r-map), 111, 119 - Emerging Roles of Cancer Stem Cells in Bladder Cancer Progression, Tumorigenesis, and Resistance to Chemotherapy: A Potential Therapeutic Target for Bladder Cancer
Abugomaa, Amira; Elbadawy, Mohamed; Yamawaki, Hideyuki; Usui, Tatsuya; Sasaki, Kazuaki
CELLS
MDPI
Bladder cancer (BC) is a complex and highly heterogeneous stem cell disease associated with high morbidity and mortality rates if it is not treated properly. Early diagnosis with personalized therapy and regular follow-up are the keys to a successful outcome. Cancer stem cells (CSCs) are the leading power behind tumor growth, with the ability of self-renewal, metastasis, and resistance to conventional chemotherapy. The fast-developing CSC field with robust genome-wide screening methods has found a platform for establishing more reliable therapies to target tumor-initiating cell populations. However, the high heterogeneity of the CSCs in BC disease remains a large issue. Therefore, in the present review, we discuss the various types of bladder CSC heterogeneity, important regulatory pathways, roles in tumor progression and tumorigenesis, and the experimental culture models. Finally, we describe the current stem cell-based therapies for BC disease.
2020年01月, 研究論文（学術雑誌）, 共同, 9, 1, DOI(公開)(r-map) - Efficacy of Juzen-taiho-to against vincristine-induced toxicity in dogs
Shinohara, Yuta; Nishino, Yoko; Yamanaka, Megumi; Ohmori, Keitaro; Elbadawy, Mohamed; Usui, Tatsuya; Sasaki, Kazuaki
JOURNAL OF VETERINARY MEDICAL SCIENCE
JAPAN SOC VET SCI
Vincristine, one of the anti-cancer drugs used in veterinary practice, has adverse hematological and gastrointestinal effects in dogs. Juzen-taiho-to is a traditional Chinese medicine used for patients with anorexia in human medicine. However, the protective effects of Juzen-taiho-to against anti-cancer drug-induced toxicity in dogs have not been investigated. We therefore examined whether the administration of Juzen-taiho-to to dogs affects gastric motility, and vincristine-induced gastrointestinal and hematological toxicity. The study was composed of three trials. In the first trial, Juzen-taiho-to (450 mg/kg/day) was orally administered to five dogs. In the second and third trials, vincristine (0.75 mg/m(2)) was intravenously administered to each dog in the absence or presence of Juzen-taiho-to (450 mg/kg/day). During these trials, gastric motility and blood parameters were assessed. Juzen-taiho-to increased gastric motility and improved vincristine-induced gastrointestinal, but not hematological, adverse effects in dogs. This study suggested that Juzen-taiho-to may be applicable for gastrointestinal care in dogs receiving chemotherapy.
2019年12月, 研究論文（学術雑誌）, 共同, 81, 12, 0916-7250, DOI(公開)(r-map), 1810, 1816 - Possible anti-oxidative effects of long-term administration of Juzen-taiho-to in dogs
Shinohara, Yuta; Oyama, Ayaka; Usui, Tatsuya; Sasaki, Kazuaki
JOURNAL OF VETERINARY MEDICAL SCIENCE
JAPAN SOC VET SCI
It is known that oxidative stress is related to disease in humans and dogs. Many traditional Chinese medicines have been reported to have anti-oxidative effects, but there are no reports that they have anti-oxidative effects in dogs. In this study, we examined the anti-oxidative effects of Juzen-taiho-to, a traditional Chinese medicine, in dogs. Five healthy female beagle dogs (38-41 months of age weighing 8.6-10.7 kg) were orally administered Juzen-taiho-to at 450 mg/kg with food for 28 days. Blood samples were taken from all five dogs on days 0, 7, 14, 21, and 28. Using the blood samples, improvement of the antioxidant level as assessed by the biological antioxidant potential (BAP), reduced oxidative stress level as assessed by derivatives of reactive oxygen metabolites (d-ROMs), and improvement of blood fluidity were examined. Regarding the antioxidant level and blood fluidity, no significant difference was observed, but the oxidative stress level on days 14, 21, and 28 was significantly lower than that on day 0. Thus, Juzen-taiho-to may have anti-oxidative effects in dogs by reducing oxidative stress and be useful for oxidative stress-related diseases in dogs.
2019年11月, 研究論文（学術雑誌）, 共同, 81, 11, 0916-7250, DOI(公開)(r-map), 1616, 1620 - Effects of a selective casein kinase 1 delta and epsilon inhibitor on Fc epsilon RI expression and IgE-mediated immediate-type cutaneous reactions in dogs
Ohno, Hikaru; Takahashi, Kaho; Yanuma, Nanako; Ogawa, Misato; Hasegawa, Ayana; Sugita, Koji; Kawano, Koji; Sasaki, Kazuaki; Shirai, Junsuke; Nagaoka, Kentaro; Ohmori, Keitaro
JOURNAL OF VETERINARY MEDICAL SCIENCE
JAPAN SOC VET SCI
The molecular clock network in mast cells has been shown to be a factor responsible for circadian regulation of allergic inflammation. PF670462 is a selective inhibitor of casein kinase 1 delta and epsilon (CK1 delta/epsilon) that control the posttranslational modification of clock proteins. The aims of this study were to evaluate the effects of PF670462 on gene and protein expression of Fc epsilon RI, the high-affinity IgE receptor, in canine mast cells and on IgE-mediated immediate-type cutaneous reactions in dogs. PF670462 decreased mRNA expression of Fc epsilon RI alpha and beta, but not gamma, and protein expression of Fc epsilon RI in a canine mast cell line. Furthermore, PF670462 suppressed IgE-mediated immediate-type cutaneous erythema in dogs. These findings indicate that CK1 delta/epsilon function as regulators for Fc epsilon RI expression and IgE-mediated cutaneous reactions in dogs.
2019年11月, 研究論文（学術雑誌）, 共同, 81, 11, 0916-7250, DOI(公開)(r-map), 1680, 1684 - Ovarian follicular changes and hemodynamics in Egyptian buffaloes under CIDR-PGF2 alpha and Ovsynch-CIDR estrus synchronization treatments
Samir, Haney; Kandiel, Mohamed M. M.; El-Maaty, Amal M. Abo; Sediqyar, Manila; Sasaki, Kazuaki; Watanabe, Gen
JOURNAL OF REPRODUCTION AND DEVELOPMENT
SOCIETY REPRODUCTION & DEVELOPMENT-SRD
This study aimed at investigating the efficacy of two protocols of estrous synchronization on follicular changes and hemodynamics. Pluriparous Egyptian buffaloes (n = 36) were synchronized either with controlled internal drug release (CIDR)-PGF2 alpha (7-days CIDR insert with PGF2 alpha injected on the 6th day; n = 18) or Ovsynch-CIDR (Ovsynch protocol concurrent with 7-days CIDR insert; n = 18). Blood sampling and ovarian ultrasound examinations (Grayscale, color and power Doppler modes) were conducted on the Day of CIDR removal, estrus, and luteal phase. Mean follicle diameter (MFD), first (1st-LF) and second (2nd-LF) largest follicle diameters, and E2 levels significantly increased in the CIDR-PGF2 alpha group at CIDR withdrawal. Ovsynch-CIDR markedly fortified higher follicle population, MFD, and 1st-LF diameter at estrus and corpus luteum (CL) volume at the luteal phase in concomitant with increases (P < 0.05) in E2 (at estrus) and P4 (at luteal phase). At CIDR removal, the blue pixels in the dominant follicle (DF) were higher (1.5 times; P = 0.054) in the Ovsynch-CIDR than in the CIDR-PGF2 alpha. At estrus, total blood flow (TBF) and power Doppler pixels (PDP) to DF(s) were noticeably higher (seven and 1.6 times; respectively) in the Ovsynch-CIDR than in CIDR-PGF2 alpha (5906 +/- 237 vs. 830 +/- 60 pixels, P < 0.01 and 5479 +/- 322 vs. 3377 +/- 19 pixels, P < 0.05; respectively). At the luteal phase, TBF and PDP to the CL increased in the Ovsynch-CIDR group than in the CIDR-PGF2 alpha group (11060 +/- 965 vs. 7963 +/- 480 pixels, 1.4 times, P = 0.05 and 18900 +/- 1350 vs. 13220 +/- 568 pixels, 1.1 times, P = 0.005; respectively). In conclusion, based on the improvement in synchronized follicular activity and hemodynamics, the Ovsynch-CIDR regimen is persuaded in Egyptian buffaloes.
2019年10月, 研究論文（学術雑誌）, 共同, 65, 5, 0916-8818, DOI(公開)(r-map), 451, 457 - Establishment of a novel experimental model for muscle-invasive bladder cancer using a dog bladder cancer organoid culture
Elbadawy, Mohamed; Usui, Tatsuya; Mori, Takashi; Tsunedomi, Ryouichi; Hazama, Shoichi; Nabeta, Rina; Uchide, Tsuyoshi; Fukushima, Ryuji; Yoshida, Toshinori; Shibutani, Makoto; Tanaka, Takaharu; Masuda, Sosuke; Okada, Rena; Ichikawa, Ryo; Omatsu, Tsutomu; Mizutani, Tetsuya; Katayama, Yukie; Noguchi, Shunsuke; Iwai, Satomi; Nakagawa, Takayuki; Shinohara, Yuta; Kaneda, Masahiro; Yamawaki, Hideyuki; Sasaki, Kazuaki
CANCER SCIENCE
WILEY
In human and dogs, bladder cancer (BC) is the most common neoplasm affecting the urinary tract. Dog BC resembles human muscle-invasive BC in histopathological characteristics and gene expression profiles, and could be an important research model for this disease. Cancer patient-derived organoid culture can recapitulate organ structures and maintains the gene expression profiles of original tumor tissues. In a previous study, we generated dog prostate cancer organoids using urine samples, however dog BC organoids had never been produced. Therefore we aimed to generate dog BC organoids using urine samples and check their histopathological characteristics, drug sensitivity, and gene expression profiles. Organoids from individual BC dogs were successfully generated, expressed urothelial cell markers (CK7, CK20, and UPK3A) and exhibited tumorigenesis in vivo. In a cell viability assay, the response to combined treatment with a range of anticancer drugs (cisplatin, vinblastine, gemcitabine or piroxicam) was markedly different in each BC organoid. In RNA-sequencing analysis, expression levels of basal cell markers (CK5 and DSG3) and several novel genes (MMP28, CTSE, CNN3, TFPI2, COL17A1, and AGPAT4) were upregulated in BC organoids compared with normal bladder tissues or two-dimensional (2D) BC cell lines. These established dog BC organoids might be a useful tool, not only to determine suitable chemotherapy for BC diseased dogs but also to identify novel biomarkers in human muscle-invasive BC. In the present study, for the 1st time, dog BC organoids were generated and several specifically upregulated organoid genes were identified. Our data suggest that dog BC organoids might become a new tool to provide fresh insights into both dog BC therapy and diagnostic biomarkers.
2019年09月, 研究論文（学術雑誌）, 共同, 110, 9, 1347-9032, DOI(公開)(r-map), 2806, 2821 - Emerging Roles of C-Myc in Cancer Stem Cell-Related Signaling and Resistance to Cancer Chemotherapy: A Potential Therapeutic Target Against Colorectal Cancer
Elbadawy, Mohamed; Usui, Tatsuya; Yamawaki, Hideyuki; Sasaki, Kazuaki
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
MDPI
Myc is a nuclear transcription factor that mainly regulates cell growth, cell cycle, metabolism, and survival. Myc family proteins contain c-Myc, n-Myc, and l-Myc. Among them, c-Myc can become a promising therapeutic target molecule in cancer. Cancer stem cells (CSCs) are known to be responsible for the therapeutic resistance. In the previous study, we demonstrated that c-Myc mediates drug resistance of colorectal CSCs using a patient-derived primary three-dimensional (3D) organoid culture. In this review, we mainly focus on the roles of c-Myc-related signaling in the regulation of CSCs, chemotherapy resistance, and colorectal cancer organoids. Finally, we introduce the various types of c-Myc inhibitors and propose the possibility of c-Myc as a therapeutic target against colorectal cancer.
2019年05月01日, 研究論文（学術雑誌）, 共同, 20, 9, DOI(公開)(r-map) - Novel Functions of Death-Associated Protein Kinases through Mitogen-Activated Protein Kinase-Related Signals.
Elbadawy M, Usui T, Yamawaki H, Sasaki K.
Int J Mol Sci.
2018年10月04日, 研究論文（学術雑誌）, 共同, 19, 10, DOI(公開)(r-map) - Effect of interleukin-1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease.
61. Ogawa M, Osada H, Hasegawa A, Ohno H, Yanuma N, Sasaki K, Shimoda M, Shirai J, Kondo H, Ohmori K.
J Vet Intern Med.
2018年05月, 研究論文（学術雑誌）, 共同, 32, 3, DOI(公開)(r-map), 1019, 1025 - Development of an Experimental Model for Analyzing Drug Resistance in Colorectal Cancer.
Elbadawy M, Usui T, Yamawaki H, Sasaki K.
Cancers (Basel)
2018年05月28日, 研究論文（学術雑誌）, 共同, 10, 6, DOI(公開)(r-map) - Effects of leucovorin (folinic acid) in the methotrexate-treated rat brain.
Ogushi N, Sasaki K, Shimoda M.
J. Vet. Med. Sci.
J. Vet. Med. Sci.
2018年05月18日, 研究論文（学術雑誌）, 共同, 80, 5, DOI(公開)(r-map), 760, 765 - Hedgehog signals mediate anti-cancer drug resistance in three-dimensional primary colorectal cancer organoid culture.
64. Tatsuya Usui, Masashi Sakurai, Koji Umata, Mohamed Elbadawy, Takashi Ohama, Hideyuki Yamawaki, Shoichi Hazama, Hiroko Takenouchi, Masao Nakajima, Ryouichi Tsunedomi, Nobuaki Suzuki, Hiroaki Nagano, Koichi Sato, Masahiro Kaneda, Kazuaki Sasaki.
Int J Mol Sci.
2018年04月06日, 研究論文（学術雑誌）, 共同, 19, 4, DOI(公開)(r-map) - Influence of glucocorticoids on time-of-day-dependent variations in IgE-, histamine-, and platelet-activating factor-mediated systemic anaphylaxis in different mouse strains
Hasegawa, Ayana; Watanabe, Miwa; Osada, Hironari; Ogawa, Misato; Ohno, Hikaru; Yanuma, Nanako; Sasaki, Kazuaki; Shimoda, Minoru; Shirai, Junsuke; Ohmori, Keitaro
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ACADEMIC PRESS INC ELSEVIER SCIENCE
A time-of-day-dependent variation in IgE-mediated passive systemic anaphylaxis was previously reported in ICR mice. In the present study, we investigated time-of-day-dependent variations in IgE-, histamine-, and platelet-activating factor (PAF)-mediated systemic anaphylaxis in C57BL/6, BALB/c, and NC/Nga mice at 9:00 h and 21:00 h, and evaluated the potential influence of glucocorticoids (GCs) on these variations. We found significant time-of-day-dependent variations in IgE-mediated systemic anaphylaxis in C57BL/6 mice, and in histamine- and PAF-mediated systemic anaphylaxis in BALB/c mice. Significant daily variations in IgE-, histamine-, and PAF-mediated systemic anaphylaxis were not observed in NC/Nga mice. Pretreatment with dexamethasone and adrenalectomy abolished the daily variations in IgE-mediated systemic anaphylaxis in C57BL/6 mice and in PAF-mediated systemic anaphylaxis in BALB/c mice, suggesting that GCs from adrenal glands are pivotal in regulating these variations. In contrast, pretreatment with dexamethasone and adrenalectomy did not abolish the daily variation in histamine-mediated systemic anaphylaxis in BALB/c mice, suggesting that GC-independent and adrenal gland-independent mechanisms are important for the variation. The present study demonstrated that time-of-day-dependent variations in systemic anaphylaxis differed among inbred mouse strains and with anaphylaxis-inducing substances. Thus, mouse strains, time of experiment, and anaphylaxis-inducing substances used must be considered to obtain appropriate experimental results. (C) 2017 Elsevier Inc. All rights reserved.
2018年01月15日, 研究論文（学術雑誌）, 共同, 495, 3, 0006-291X, DOI(公開)(r-map), 2184, 2188 - Effectiveness of the liver micronucleus assay using juvenile mice
Nagasue, Ritsuko; Murata, Ikue; Sasaki, Kazuaki; Sakai, Rina; Miyajima, Hirofumi; Shimoda, Minoru
JOURNAL OF VETERINARY MEDICAL SCIENCE
JAPAN SOC VET SCI
This study investigated the effectiveness of the liver micronucleus (MN) assay using juvenile mice. Therefore, we analyzed various hepatic cytochrome P450 (CYP)-mediated activities of ethoxyresorufin O-deethylation, pentoxyresorufin O-dealkylation, tolbutamide hydroxylation, bufuralol 1'-hydroxylation, aniline hydroxylation and midazolam 4-hydroxylation by CYP1A, CYP2B, CYP2C, CYP2D, CYP2E and CYP3A, respectively, in non-treated male ICR mice aged between 3 and 8 weeks. The enzyme efficiency levels in 3-and 4-week-old mice were approximately similar to or higher than those in 8-week-old mice, except for CYP1A and CYP2E in 3-and 4-week-old mice, respectively. Since these results suggest that juvenile mice have sufficient activities for most CYP enzymes, we also conducted a liver MN assay using diethylnitrosamine (DEN), a rodent hepatocarcinogen, on male ICR mice aged between 3 and 6 weeks. A peripheral blood (PB) MN assay was performed simultaneously in 4-week-old mice. Assays incorporating DEN produced positive results in 3-and 4-week-old mice and showed a dose-dependent increase in the micronucleated hepatocyte frequencies at 4 weeks. Both the liver MN assay in 5-and 6-week-old mice and the PB MN assay had negative results when using DEN. These results suggest that 3-and 4-week-old mice have micronuclei-inducing potential in the liver to detect genotoxic compounds using the liver MN assay.
2017年07月, 研究論文（学術雑誌）, 共同, 79, 7, 0916-7250, DOI(公開)(r-map), 1310, 1317 - CAN a P-gp modulater assit in the control of methotrexate concentrations in the rat brain? -inhibitory effects of rhodamine 123, a specific substrate for P-gp, on methotrexate execretion from the rat brain and its optimal route of administration
Ogushi, Naofumi; Sasaki, Kazuaki; Shimoda, Minoru
JOURNAL OF VETERINARY MEDICAL SCIENCE
JAPAN SOC VET SCI
Although methotrexate (MTX) is mainly transported by reduced folate carrier, P-gp and MRP1 may also be involved in its transport. In our previous study, a potent P-gp and MRP1 modulator, Cyclosporine A, potentiated MTX concentration in rat brain. Since it is important for MTX therapy for brain tumor to clarify which transporter is dominant, we herein determined whether the specific P-gp substrate, rhodamine123 (Rhol23), potentiates the transport and retention of MTX in the brain. Rhol 23 was injected intravenously or intrathecally into rats immediately after injection of MTX. 6 or 12 hr after the MTX injection, brains were isolated just after the sampling of cerebrospinal fluid (CSF). Blood was also collected intermittently. MTX concentrations were determined in plasma, CSF and the brain using high-performance liquid chromatography with UV detection. When MTX was intravenously injected, Rho123 didn't affect MTX concentrations in the brain. However, Rho123 resulted in significantly higher MTX concentrations in the brain at 12 hr after injection when MTX was intrathecally injected. It is suggested that Rho123 inhibits the excretion of MTX from the brain, but does not potentiate its distribution from the blood into the brain. This reveals that P-gp can be one of the major transporters of MTX in rat brain. Therefore, treatments with P-gp modulators may contribute to intrathecal MIX therapy for brain tumor. Since plasma concentration-time curves of MTX were not affected by Rhol 23, treatments with P-gp modulators may not potentiate the adverse effects of MTX.
2017年02月, 研究論文（学術雑誌）, 共同, 79, 2, 0916-7250, DOI(公開)(r-map) - Expression of epithelial cell-derived cytokine genes in the duodenal and colonic mucosae of dogs with chronic enteropathy
Osada, Hironari; Ogawa, Misato; Hasegawa, Ayana; Nagai, Makoto; Shirai, Junsuke; Sasaki, Kazuaki; Shimoda, Minoru; Itoh, Hiroshi; Kondo, Hirotaka; Ohmori, Keitaro
JOURNAL OF VETERINARY MEDICAL SCIENCE
JAPAN SOC VET SCI
It remains unclear whether epithelial cell-derived cytokines, including interleukin (IL)-25, IL-33 and thymic stromal lymphopoietin (TSLP), contribute to development of canine chronic enteropathy (CE), which includes antibiotic-responsive enteropathy (ARE), food-responsive enteropathy (FRE) and inflammatory bowel disease (IBD). In the present study, we examined mRNA expression of il-25,il-33 and tslp in the duodenal and colonic mucosae of dogs with ARE, FRE and IBD. Real-time PCR analysis revealed that mRNA expression of il-33 was significantly lower in the duodenum in dogs with FRE than in healthy dogs. The results suggest that epithelial cell derived cytokines may not be an inducer of Th2-type immunity in the gut of dogs with CE, and decreased expression of IL-33 may be involved in induction of FRE. Further studies are required to clarify roles of epithelial cell-derived cytokines, especially IL-33, in the pathogenesis of canine CE.
2017年02月, 研究論文（学術雑誌）, 共同, 79, 2, 0916-7250, DOI(公開)(r-map) - Can a P-gp modulator assist in the control of methotrexate concentrations in the rat brain?: inhibitory effects of rhodamine 123, a specific substrate for P-gp, on methotrexate excretion from the rat brain and its optimal route of administration.
Ogushi N, Sasaki K, Shimoda M.
J Vet Med Sci
2016年12月, 研究論文（学術雑誌）, 共同, Dec 5 - Expression of epithelial cell-derived cytokine genes in the duodenal and colonic mucosae of dogs with chronic enteropathy.
Osada H, Ogawa M, Hasegawa A, Nagai M, Shirai J, Sasaki K, Shimoda M, Itoh H, Kondo H, Ohmori K.
J Vet Med Sci.
2016年12月, 研究論文（学術雑誌）, 共同, Dec 29 - Rapid absorption of diclofenac and acetaminophen after their oral administration to cattle.
Sawaguchi A, Sasaki K, Miyanaga K, Nakayama M, Nagasue M, Shimoda M.
J Vet Med Sci.
2016年10月, 研究論文（学術雑誌）, 共同, 78, 9, 1481, 1485 - Characterization of platelet-activating factor-induced cutaneous edema and erythema in dogs.
Watanabe M, Osada H, Shimizu S, Goto S, Nagai M, Shirai J, Sasaki K, Shimoda M, Itoh H, Ohmori K.
Am J Vet Res.
2016年09月, 研究論文（学術雑誌）, 共同, 77, 9, 969, 975 - Influence of glucocorticoids on a time-of-day-dependent variation in intradermal reactivity to histamine in dogs.
Goto S, Shimizu S, Watanabe M, Osada H, Sasaki K, Shimoda M, Nagai M, Shirai J, Itoh H, Ohmori K.
Vet J.
2016年08月, 研究論文（学術雑誌）, 共同, 214, 86, 90 - Oral absorption profiles of sulfonamides in Shiba goats: a comparison among sulfadimidine, sulfadiazine and sulfanilamide.
Elbadawy M, Ishihara Y, Aboubakr M, Sasaki K, Shimoda M.
J Vet Med Sci.
2016年07月, 研究論文（学術雑誌）, 共同, 78, 6, 1025, 1029 - Oral pharmacokinetics of acetaminophen to evaluate gastric emptying profiles of Shiba goats.
Elbadawy M, Sasaki K, Miyazaki Y, Aboubakr M, Khalil WF, Shimoda M.
J Vet Med Sci.
2015年10月, 研究論文（学術雑誌）, 共同, 77, 10, 1331, 4 - Effect of cyclosporin on distribution of methotrexate into the brain of rats
Ogushi N, Sasaki K, Shimoda M
J Vet Med Sci
2015年10月, 研究論文（学術雑誌）, 共同, 77, 9, 1171, 3 - Oral pharmacokinetics of the acidic drugs, diclofenac and sulfamonomethoxine in male Shiba Goats
Elbadawy M, Sakiyama T, Abohatab R, Sasaki K, Shimoda M
J. Vet. Med. Sci.
2015年07月, 研究論文（学術雑誌）, 共同, 77, 1, 21, 6. - Effect of a single injection of gonadotropin-releasing hormone (GnRH) and human chorionic gonadotropin (hCG) on testicular blood flow measured by color doppler ultrasonography in male Shiba goats.
Samir H, Sasaki K, Ahmed E, Karen A, Nagaoka K, El Sayed M, Taya K, Watanabe G.
J Vet Med Sci.
2015年05月, 研究論文（学術雑誌）, 共同, 77, 5, 549, 56 - Possible drug-drug interaction in dogs and cats resulted from alteration in drug metabolism: A mini review.
Sasaki K, Shimoda M
J Adv Res
2015年05月, 共同, 6, 3, 383, 92 - 犬肝細胞癌の診断におけるソナゾイドによる造影超音波検査の有用性—造影超音波所見と病理組織所見との対比—
小林正行, 坂東秀紀, 山本慎也, 中尾周, 佐々木一昭, 伊藤博, 町田登
動物臨床医学 巻：21 号：2 ページ：054-059
2012年06月, 研究論文（学術雑誌）, 共同, 21, 2, 054, 059 - Ultrasound safety with midfrequency transcranial sonothrombolysis: preliminary study on normal macaca monkey brain.
Shimizu J, Fukuda T, Abe T, Ogihara M, Kubota J, Sasaki A, Azuma T, Sasaki K, Shimizu K, Oishi T, Umemura S, Furuhata H.
Ultrasound Med Biol.
2012年06月, 研究論文（学術雑誌）, 共同, 38, 6, 1040, 1050 - Sonodynamic therapy with 5-aminolevulinic acid and focused ultrasound for deep-seated intracranial glioma in rat.
Ohmura T, Fukushima T, Shibaguchi H, Yoshizawa S, Inoue T, Kuroki M, Sasaki K, Umemura S.
Anticancer Res.
2011年07月, 研究論文（大学，研究機関紀要）, 共同, 31, 7, 2527, 2533 - Magnetocardiogram of Medicine-Induced Rabbit: ST-T Change in Current-Arrow Map of Hypoxia
Akihiko Kandori, Yusuke Seki, Kazuaki Sasaki, Koji Hirata, Tsuyoshi Miyashita
日本生体磁気学会論文誌
2010年05月, 研究論文（学術雑誌）, 共同, 22, 2, 11, 19 - Effects of several pyrethroids on hepatic cytochrome p450 activities in rats.
Abdou R, Sasaki K, Khalil W, Shah S, Murasawa Y, Shimoda M.
J Vet Med Sci..
2010年05月, 研究論文（学術雑誌）, 共同, 72, 4, 425, 433 - Inhibitory effects of ketoconazole, cimetidine and erythromycin on hepatic CYP3A activities in cats.
Shah SS, Sasaki K, Hayashi Y, Motoyama S, Helmi AR, Khalil WF, Shimoda M.
J Vet Med Sci..
2009年09月, 研究論文（学術雑誌）, 共同, 71, 9, 1151, 1159. - Effects of focused ultrasound sonodynamic treatment on the rat blood-brain barrier.
Yoshino S, Fukushima T, Hayashi S, Nonaka M, Ogawa K, Sasaki K, Umemura S.
Anticancer Res.
2009年03月, 研究論文（学術雑誌）, 共同, 29, 3, 889, 895 - Sonodynamic therapy consisting of focused ultrasound and a photosensitizer causes a selective antitumor effect in a rat intracranial glioma model.
Nonaka M, Yamamoto M, Yoshino S, Umemura S, Sasaki K, Fukushima T.
Anticancer Res.
2009年03月, 研究論文（学術雑誌）, 共同, 29, 3, 943, 950 - Sequential change in rat femoral artery blood flow and tissue degeneration after exposure to high-intensity focused ultrasound
Ishikawa T, Okai T, Sasaki K, Umemura S, Miyazaki N, Kushima M,Aoki H, Ichihara M, Ichizuka K.
J Med Ultrasonics
2008年12月, 研究論文（学術雑誌）, 共同, 35, 4, 177, 182 - Sonodynamic therapy on chemically induced mammary tumor: pharmacokinetics, tissue distribution and sonodynamically induced antitumor effect of gallium-porphyrin complex ATX-70.
Yumita, N., Okuyama, N., Sasaki, K., Umemura, S.
Cancer Chemother. Pharmacol.
2007年11月, 研究論文（学術雑誌）, 共同, 60, 6, 891, 897 - Characterization of cytochrome P450-mediated drug metabolism in cats
Shah, S. S., Sanda, S., Regmi, N. L., Sasaki, K., Shimoda, M.
J. vet. Pharmacol. Therap.
2007年10月, 研究論文（学術雑誌）, 共同, 30, 422, 428 - Motion-compensated frame-accumulating method to improve quality of carotid artery images.
Yoshikawa, H., Azuma, T., Sasaki, K., Kawabata, K., and Umemura, S.
Jpn. J. Appl. Phys.
2007年07月, 研究論文（学術雑誌）, 共同, 46, 7B, 4834, 4840 - Application of high-intensity focused ultrasound for umbilical artery occlusion in a rabbit model.
Ichizuka K, Ando S, Ichihara M, Ishikawa T, Uchiyama N, Sasaki K, Umemura S, Matsuoka R, Sekizawa A, Okai T, Akabane T, Kushima M.
Ultrasound Obstet Gynecol.
2007年07月, 研究論文（学術雑誌）, 共同, 30, 1, 47, 51 - Synthesis of amphiphilic derivatives of rose bengal and their tumor accumulation.
Sugita N, Kawabata KI, Sasaki K, Sakata I, Umemura S.
Bioconjug Chem
2007年03月, 研究論文（学術雑誌）, 共同, 18, 3, 866, 873 - Blood flow occlusion via ultrasound image-guided high-intensity focused ultrasound and its effect on tissue perfusion.
Ichihara M, Sasaki K, Umemura S, Kushima M, Okai T.
Ultrasound Med Biol
2007年03月, 研究論文（学術雑誌）, 共同, 33, 3, 452, 459. - Effect of echo-guided high-intensity focused ultrasound ablation on localized experimental tumor.
Sasaki K, Medan MS, Azuma T, Kawabata K, Shimoda M, Umemura S.
J Vet Med Sci
2006年10月, 研究論文（学術雑誌）, 共同, 68, 10, 1069, 1074 - Chronic effect of transrectal split-focus ultrasonic ablation on canine prostatic tissue.
Sasaki K, Azuma T, Kawabata K, Umemura S, Tanaka R, Yamane Y, Shimoda M.
J Vet Med Sci
2006年08月, 研究論文（学術雑誌）, 共同, 68, 8, 839, 845 - Dynamic and Precise Visualization of Contrast Agent in Blood Vessels with Motion Correction.
Yoshikawa H, Azuma T, Sasaki K, Kawabata K, Umemura S.
Jpn. J. Appl. Phys.
2006年05月, 研究論文（学術雑誌）, 共同, 45, 4754, 4760 - Immunization of goats against Inhibin increased follicular development and ovulation rate.
Sasaki K, Medan MS, Watanabe G, Sharawy S, Taya K.
J. Reprod. Dev.
2006年04月, 研究論文（学術雑誌）, 共同, 52, 4, 543, 550 - 医用超音波における微小気泡の非線形挙動の応用
梅村晋一郎 川畑健一 佐々木一昭
電子情報通信学会論文誌
2006年01月, 研究論文（学術雑誌）, 共同, J89-A, 1 - Acceleration of ultrasonic tissue heating by microbubble agent.
Umemura S, Kawabata K, Sasaki K
IEEE Trans. Ultrason. Ferroelect. Freq. Control.
2005年10月, 研究論文（学術雑誌）, 共同, 52, 10, 1690, 1698 - Follicular and hormonal dynamics during the estrous cycle in goats.
Medan MS, Watanabe G, Sasaki K, Groom NP, Sharawy S, Taya K.
J. Reprod. Dev.
2005年08月, 研究論文（学術雑誌）, 共同, 51, 4 - 強出力集束超音波を用いた栄養血管閉塞による子宮筋腫の無侵襲治療装置の開発と臨床手技の確立を目指した基礎的研究
市塚清健、岡井崇、藤原礼、石川哲也、市原三義、梅村晋一郎、佐々木一昭、川畑健一、馬場一憲
日本超音波医学会誌
2005年06月, 共同, 32, 3, 339, 346 - Overexpression of heat shock proteins in pallido-nigral axonal spheroids of nonhuman aged primates.
Fukuda T, Shimizu J, Furuhata H, Abe T, Shimizu K, Oishi T, Ogihara M, Kubota J, Sasaki A, Sasaki K, Azuma T, Umemura S
Acta Neuropathol (Berl)
2005年06月, 研究論文（学術雑誌）, 共同, 110, 2, 145, 150 - HIFU照射による動脈閉塞に対する超音波造影剤の効果及びそれによる血管内皮の組織学的変化.
内山心美、石川哲也、安藤智、市原三義、松岡隆、市塚清隆、岡井崇、佐々木一昭、梅村晋一郎、九島巳樹
昭和医学会雑誌
2005年01月, 研究論文（大学，研究機関紀要）, 共同, 65, 165, 173 - Sonodynamic therapy on chemically induced mammary tumor: Pharmacokinetics, tissue distribution and sonodynamically induced antitumor effect of porfimer sodium.
Yumita N, Okuyama N, Sasaki K, Umemura S
Cancer Sci.
2004年09月, 研究論文（学術雑誌）, 共同, 95, 9, 765, 769 - Sonodynamic treatment of murine tumor through second-harmonic superimposition.
Sasaki K, Kawabata K, Yumita N, Umemura S.
Ultrasound Med Biol
2004年09月, 研究論文（学術雑誌）, 共同, 30, 9, 1233, 1238 - Early pregnancy diagnosis by means of ultrasonography as a method of improving reproductive efficiency in goats.
Medan MS, Watanabe G, ABSY G, Sasaki K, Sharawy S, Taya K
J. Reprod. Dev.
2004年08月, 研究論文（学術雑誌）, 共同, 50, 4, 391, 397 - Transrectal ultrasonic diagnosis of ovarian follicular cysts in goats and treatment with GnRH.
Medan MS, Watanabe G, Sasaki K, Taya K.
Domestic Animal Endocrinology
2004年08月, 研究論文（学術雑誌）, 共同, 27, 2, 115, 124 - HIFUを用いた栄養血管閉塞による子宮筋腫の無侵襲治療に関する基礎研究.
石川哲也、岡井崇、佐々木一昭 梅村晋一郎
別刷：医学の歩み 超音波医学最前線 新技術と臨床応用
2004年01月, 共同, 187, 191 - 集束超音波治療とその課題を克服するアプローチ.
梅村晋一郎、川畑健一、佐々木一昭、 杉田奈巳
別刷：医学の歩み 超音波医学最前線 新技術と臨床応用
2004年01月, 共同, 182, 186 - Functional and histological changes in rat femoral arteries by HIFU exposure.
Ishikawa T, Okai T, Sasaki K, Umemura S, Fujiwara R, Kushima M, Ichihara M, Ichizuka K.
Ultrasound Med Biol
2003年10月, 研究論文（学術雑誌）, 共同, 29, 10, 1471, 1477 - Effects of passive immunization of goats against inhibin on follicular development, hormone profile and ovulation rate
Medan MS, Watanabe G, Sasaki K, Nagura Y, Sakaime H, Fujita M, Sharawy S, Taya K.
Reproduction
2003年05月, 研究論文（学術雑誌）, 共同, 125, 5, 751, 757 - Ovarian and hormonal response of female goats to active immunization against inhibin.
Medan MS, Watanabe G, Sasaki K, Nagura Y, Sakaime H, Fujita M, Sharawy S, Taya K.
J Endocrinol
2003年05月, 研究論文（学術雑誌）, 共同, 177, 2, 287, 294 - Effect of split-focus approach on producing larger coagulation in swine liver.
Sasaki K, Azuma T, Kawabata K, Shimoda M, Kokue E, Umemura S.
Ultrasound Med Biol
2003年04月, 研究論文（学術雑誌）, 共同, 29, 4, 591, 599 - Ovarian Dynamics and Their Associations with Peripheral Concentrations of Gonadotrophins, Ovarian Steroids and Inhibin During the Estrous Cycle in Goats.
Medan MS, Watanabe G, Sasaki K, Sharawy S, Groome NP, Taya K.
Biol Reprod
2003年02月, 研究論文（学術雑誌）, 共同, 69, 1, 57, 63 - HIFU照射の動脈血流に及ぼす影響に関する研究.
石川哲也、藤原礼、市原三義、市塚清健、岡井崇、佐々木一昭、梅村晋一郎、九島巳樹
昭和医学会雑誌
2003年01月, 研究論文（学術雑誌）, 共同, 63, 279, 285 - Sonodynamic effect of erythrosin B on sarcoma 180 cells in vitro.
Yumita N, Kawabata K, Sasaki K, Umemura S.
Ultrasonics Sonochemistry
2002年10月, 研究論文（学術雑誌）, 共同, 9, 5, 259, 265 - Artery blood flow occlusion by high intensity focused ultrasound and histological evaluation of its effect on arteries and surrounding tissues.
Fujiwara R, Sasaki K, Ishikawa T, Suzuki M, Umemura S, Kushima M, Okai T.
J Med Ultrasonics
2002年09月, 研究論文（学術雑誌）, 共同, 29, 85, 90 - MRI-compatible ultrasound therapeutic system.
Azuma T, Sasaki K, Kawabata K, Osada A, Itagaki H, Komura K, Takahashi T, Ishida K, Sato Y, Umemura S.
Jpn. J. Appl. Phys.
2002年05月, 研究論文（学術雑誌）, 共同, 41, 3308, 3312 - Pharmacokinetic study of a Ga-porphyrin complex photo- and sono-sensitizer, ATX-70, in tumor bearing mice.
Sasaki K, Yumita N, Nishigaki R, Sakata I, Nakajima S, Umemura S.
Jpn J Cancer Res.( Cancer Sci.)
2001年09月, 研究論文（学術雑誌）, 共同, 92, 9, 989, 995 - 集束超音波治療技術.
梅村晋一郎、川畑健一、佐々木一昭、杉田奈巳、東隆、弓田長彦
医用物理
2001年01月, 共同, 12, 150, 156 - 実験乳癌に対する音響化学療法の効果
奥山伸男、笹本修一、加藤信秀、高本啓吾、山崎史朗、辻本志朗、弓田長彦、西垣隆一郎、佐々木一昭、梅村晋一郎
日本超音波医学会誌
2000年12月, 研究論文（学術雑誌）, 共同, 27, 12, 1465, 1469 - Intracorporeal imaging and differentiation of living tissue with an ultra-high-frequency ultrasound probe.
Yokosawa K, Sasaki K, Umemura S, Shinomura R, Ishikawa S, Sano S, Ito Y.
Ultrasound Med Biol
2000年05月, 研究論文（学術雑誌）, 共同, 26, 4, 503, 507 - Binding characteristics of folate to high affinity folate binding protein purified from porcine serum.
Natsuhori M, Okada M, Ida R, Sasaki K, Shimoda M, Kokue E.
J Vet Med Sci.
1999年07月, 研究論文（学術雑誌）, 共同, 61, 7, 743, 748 - 低侵襲治療への音響化学的アプローチ.
梅村晋一郎、川畑健一、佐々木一昭、弓田長彦、西垣隆一郎、梅村甲子郎
MEDICAL IMAGING TECHNOLOGY
1998年12月, 共同, 16, 353, 358 - Antitumor effect sonodynamically induced by focused ultrasound in combination with Ga-porphyrin complex.
Sasaki K, Yumita N, Nishigaki R, Umemura S.
Jpn J Cancer Res.( Cancer Sci.)
1998年04月, 研究論文（学術雑誌）, 共同, 89, 4, 452, 456 - In vitro and in vivo enhancement of sonodynamically active cavitation by second-harmonic superimposition.
Umemura S, Kawabata K, Sasaki K.
J Acoust Soc Am.
1997年01月, 研究論文（学術雑誌）, 共同, 101, 1, 569, 577 - Sonodynamically induced antitumor effect of gallium-porphyrin complex by focused ultrasound on experimental kidney tumor.
Yumita N, Sasaki K, Umemura S, Yukawa A, Nishigaki R.
Cancer Lett.
1997年01月, 研究論文（学術雑誌）, 共同, 112, 1, 79, 86 - Recent advances in sonodynamic approach to cancer therapy.
Umemura S, Kawabata K, Sasaki K, Yumita N, Umemura K, Nishigaki R.
Ultrasonics Sonochemistry
1996年11月, 研究論文（学術雑誌）, 共同, 3, 3, S187, S191 - Enhancement of sonodynamic tissue damage production by second-harmonic superimposition: Theoretical analysis of its mechanism.
Umemura S, Kawabata K, Sasaki K.
IEEE Trans. Ultrason. Ferroelect. Freq. Control.
1996年06月, 研究論文（学術雑誌）, 共同, 43, 6, 1055, 1062 - Sonodynamically induced antitumor effect of a gallium-porphyrin complex, ATX-70.
Yumita N, Sasaki K, Umemura S, Nishigaki R
Jpn J Cancer Res.( Cancer Sci.)
1996年03月, 研究論文（学術雑誌）, 共同, 87, 3, 310, 316 - ガン治療へ音響化学的アプローチ.
梅村晋一郎、川畑健一、佐々木一昭、弓田長彦、岡野幸浩、金内正浩、厚川俊明、岸晴美、谷川志美、梅村甲子郎、西垣隆一郎
化学工業
1996年01月, 共同, 47, 8, 638, 643 - Role of high-affinity folate-binding protein in the plasma distribution of tetrahydrofolate in pigs.
Sasaki K, Natsuhori M, Shimoda M, Saima Y, Kokue E.
Am J Physiol.
1996年01月, 研究論文（学術雑誌）, 共同, 270, 1Pt2, R105, 110 - 実験肝腫瘍に対する音響化学療法の試み
青柳徹二、飯田和成、松尾賢二、野中博子、佐々木一昭、川畑健一、梅村晋一郎、弓田長彦、西垣隆一郎、梅村甲子郎
日本超音波医学会誌
1993年07月, 研究論文（学術雑誌）, 共同, 20, 436, 444 - Contribution of plasma protein binding to the stability of tetrahydrofolate in pig plasma.
Sasaki, K., Shimoda, M., Aoki, Y., Natsuhori, M., and Kokue, E.
Adv. Exp. Med. Biol.
1993年01月, 共同, 338, 749, 752
研究発表、招待講演等
- Pharmacokinetics of orally administered itraconazole in king penguins (Aptenodytes patagonicus) after single doses
2021年09月22日, 口頭発表（一般） - Oral absorption profiles of sulfonamides in Shiba goats
The 14th International EAVPT Congress
2018年06月25日, ポスター発表 - イヌの全身麻酔時における吸入気酸素濃度（FiO2）が肺に与える影響
第156回日本獣医学会学術集会
2013年09月20日, 口頭発表（一般） - Evaluation of gastric emptying profiles of Shiba goats by oral pharmacokinetics of acetaminophen
第156回日本獣医学会学術集会
2013年09月20日, 口頭発表（一般） - ウシにおけるアセトアミノフェン経口投与後の薬物吸収
第156回日本獣医学会学術集会
2013年09月20日, 口頭発表（一般） - 発熱ウシにおけるジクロフェナク経口投与後の血漿中濃度と解熱効果
第156回日本獣医学会学術集会
2013年09月20日, 口頭発表（一般） - ウシにおけるジクロフェナク経口投与後の薬物動態
第156回日本獣医学会学術集会
2013年09月20日, 口頭発表（一般） - ウサギ実験的腱損傷の治癒過程に及ぼすGSK-3阻害剤の影響
第156回日本獣医学会学術集会
2013年09月20日, 口頭発表（一般） - イヌにおける徐放性製剤経口投与の意義
第154回日本獣医学会学術集会
2012年09月15日, 口頭発表（一般） - ウサギ実験的腱損傷の治癒過程のおける腱関連遺伝子発現の解析
第154回日本獣医学会学術集会
2012年09月15日, 口頭発表（一般） - 肝臓の薬物代謝酵素研究の展望
第18回日本野生動物医学会大会
2012年08月26日, 口頭発表（一般） - Pharmacokinetics of Acidic Drugs, Diclofenac And Sulfamonomethoxine in Ruminants
The 12th International EAVPT Congress
2012年07月12日, ポスター発表 - Biliary Excretion of Several Fluoroquinolones After Intravenously Injection in Rabbits
The 12th International EAVPT Congress
2012年07月12日, ポスター発表 - ヤギのおけるジクロフェナクの経口投与後の薬物吸収
第152回日本獣医学会学術集会
2011年09月20日, 口頭発表（一般） - 犬肝細胞癌の診断におけるソナゾイド造影超音波検査の有用性
第30回動物臨床医学会
2009年11月21日, 口頭発表（一般） - 造影超音波による腫瘍流入時間差評価の基礎的検討
第30回動物臨床医学会
2009年11月21日, 口頭発表（一般） - 犬におけるステロイド系抗炎症薬の投与が肝ＣＹＰ酵素活性に及ぼす影響について
第148回日本獣医学会学術集会
2009年09月25日, 口頭発表（一般） - EFFECTS OF SEVERAL PYRETHROIDS ON THE ACTIVITIES OF CYTOCHROME P450 IN RATS
EAVPT2009
2009年07月12日, ポスター発表 - 動きベクトル場の解析による境界検出イメージング
日本超音波医学会 第82回学術集会
2009年05月22日, その他 - 相変化ナノ液滴の腫瘍内分布における粒子径の影響
日本超音波医学会 第82回学術集会
2009年05月22日, その他 - 超音波造影剤の流入時間差に基づく新規映像法と肝腫瘍鑑別への有効性検討
日本超音波医学会 第82回学術集会
2009年05月22日, その他 - 相変化ナノ液滴による分子診断と治療の可能性
日本超音波医学会 第82回学術集会
2009年05月22日, その他 - Tissue boundary imaging based on eigenvalue decomposition.
The 29th Symposium on Ultrasonic Electronics(USE2008)
2008年11月11日, ポスター発表 - Inflow-Time Mapping of Ultrasonic Contrast Agents for Differential Diagnosis of Liver Tumor
2008 IEEE International Ultrasonics Symposium (IUS)
2008年11月02日, ポスター発表 - 犬におけるステロイドのCYP活性に対する阻害作用
第146回日本獣医学会学術集会
2008年09月24日, 口頭発表（一般） - ラットにおける消炎ステロイド投与の肝CYP酵素活性に対する影響
第146回日本獣医学会学術集会
2008年09月24日, 口頭発表（一般） - Influence of ethoxyquin on activities of cytochrome P450 (CYP)
29th WVC2008
2008年07月30日, ポスター発表 - 体動補正画像加算技術による動脈相血管画像の高画質化
日本超音波医学会 第81回学術集会
2008年05月23日, 口頭発表（一般） - うさぎ肝臓がんモデルを用いる相変化ナノ造影剤の生体効果検証
日本超音波医学会 第81回学術集会
2008年05月23日, 口頭発表（一般） - 動きベクトルを用いた境界検出イメージング
日本超音波医学会 第81回学術集会
2008年05月23日, その他 - Inhibitory effects of several CYP3A inhibitors on CYP3A activity in cats
2nd Asian Pacific Regional ISSX Meeting
2008年05月11日, ポスター発表 - エトキシキンが肝ＣＹＰ酵素活性におよぼす影響
第28回動物臨床医学会
2007年11月17日, 口頭発表（一般） - Boundary Imaging Based on Tissue Motion Vector 0.2
The 28th Symposium on Ultrasonic Electronics(USE2007)
2007年11月16日, ポスター発表 - High-Contrast Imaging with Microbubble by Accumulating Frames with Motion Compensation on Pulse-Inversion
2007 IEEE International Ultrasonics Symposium
2007年10月28日, ポスター発表 - ネコにおけるフルオロキノロンのCYP1A にお よぼす影響 0.1
第144回日本獣医学会学術集会
2007年09月03日, 口頭発表（一般） - Characterization of cytochrome P450-mediated drug metabolism in cats 0.1
第144回日本獣医学会学術集会
2007年09月03日, 口頭発表（一般） - HIFU照射による子宮筋腫栄養血管の閉塞
日本超音波医学会 第80回学術集会
2007年05月18日, 口頭発表（一般） - 体動補正を用いる像加算技術のアーチファクト低減に関する検討
日本超音波医学会 第80回学術集会
2007年05月18日, 口頭発表（一般） - 3パルス法の造影剤/生体非線形成分峻別比に関する検討 0.1
日本超音波医学会 第80回学術集会
2007年05月18日, 口頭発表（一般）
外部研究資金等
- ①-4 相変化要超音波及び照射システムの中・大動物による効果検証②-3 治療用超音波及び照射システムの中・大動物による治療効果検証
受託研究, 自 2009年04月01日, 至 2010年02月28日 - 次世代超音波組織性状イメージング技術開発のための動物臨床データ取得方法の検討及び動物臨床データベース作成
共同研究, 自 2008年04月01日, 至 2009年03月31日
委員歴
- 内閣府
食品委員会専門委員
自 20220401, 至 20240331 - 農林水産省
獣医事審議会専門委員
自 20220401, 至 20230331 - 農林水産省
動物医薬品検査所プロジェクト研究等評価委員会 評価委員
自 20210401, 至 20230331 - 農林水産省
獣医事審議会専門委員
自 20210401, 至 20220331 - 内閣府
食品安全委員会専門委員
自 20200401, 至 20220331 - 農林水産省
動物医薬品検査所プロジェクト研究等評価委員会 評価委員
自 20190401, 至 20210330 - 厚労省
薬事・食品衛生審議会専門委員
自 20190201, 至 20210131 - 内閣府
食品安全委員会専門委員
自 20180401, 至 20200331 - 農林水産省
動物医薬品検査所プロジェクト研究等評価委員会 評価委員
自 20170401, 至 20190330 - 厚労省
薬事・食品衛生審議会専門委員
自 20170201, 至 20190131 - 内閣府
食品安全委員会専門委員
自 20160401, 至 20180331 - 厚労省
薬事・食品衛生審議会専門委員
自 20150201, 至 20170131 - 農林水産省
獣医事審議会専門委員
自 20140401, 至 20170331 - 農林水産省
薬事・食品衛生審議会専門委員
自 20130201, 至 20150131 - 農林水産省
薬事・食品衛生審議会専門委員
自 20110201, 至 20130131
メディア報道
- 高速培養可能で3Dオルガノイドの特徴をもつ「2.5次元培養細胞」の作出に成功－東京農工大
東京農工大学大学院共同獣医学専攻のアミラ・アブゴマ大学院生（博士課程1年）、同大農学研究院の佐々木一昭准教授、臼井達哉特任講師らの研究チームが、膀胱がんの3次元（3D）オルガノイド培養組織から細胞を分離し、新たな培養液成分を用いてゲルフリーの2次元環境下（平面上）で高速培養が可能かつ、3Dオルガノイド培養組織の特徴を維持する新たな2.5次元培養細胞（2.5Dオルガノイド）を作出することに成功したことが紹介される。
QLifePro
自 2020年06月12日, 至 2020年06月12日